Legionella pneumophila

Two L. pneumophila isolates of serogoup 1 are being sequenced in Genoscope : One strain is a newly emerging epidemic strain in France and the other one is a L. pneumophila serogroup 1 isolate present since several years in the water system of a French hospital. The genome size for each strain is estimated at 3.5 Mb.

Sequence coverage for each genome is 15X and is being performed by the pyrosequencing technology. In addition, one library is being sequenced via the Sanger technology: 10,000 clones with 10 kb inserts cloned in the low copy vector pCNS.

A manual finishing stage will be performed at Genoscope.

Contacts: Valérie Barbe (Genoscope) - Carmen Buchrieser (Institut Pasteur)