Leptopilina boulardi Filamentous Virus

The project has two sides : a genomic side, and a transcriptomic side.

The genomic part aims at sequencing the genome of the virus LbFV. Starting from a partial purification of the virus, a whole genome amplification (WGA) will be performed. This library will be sequenced using 454-FLX technology, leading to a presumed depth of 100x.

The transcriptomic part will consist of one normalized cDNA library, to generate the first and most comprehensive draft of the transcriptome of L. boulardi, and four non-normalized cDNA libraries (head+thorax or abdomen, infected or uninfected). The normalized library will be sequenced using 454-Titanium technology (1/4 or ½ run) and should generate a depth of around 2-3. The four non-normalized libraries will be sequenced using Solexa (1 lane each) or Solid Applied Biosystem technology (equivalent reads amount). The 75bp sequences generated by Solexa or Solid technologies will be mapped to genes obtained from the normalized library or to gene predictions from public databases (obtained on related species: Nasonia, Apis).

Contact: Julie Poulain (Genoscope) - Julien Varaldi (Université Claude Bernard Lyon 1)