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The phenotyping which we have undertaken consists of detecting variations in growth characteristics on semisolid media between different mutants from our collection. This medium is a so-called “minimal” medium which has been supplemented with one or more metabolites which have been identified as intermediates in a given biosynthetic pathway. High throughput phenotyping of the mutant collection on 24 carbon sources is currently in progress.
On the left : Spotting of 1152 A. baylyi mutants per plate. Each plate of gelose contains a particular source of carbon.
On the right : One of the plate of gelose after 24h of growth.
This analysis will allow classification of mutants which present a phenotype which differs from wild type in the same metabolic context (i.e. metabolic pathways). An example concerning genes implicated in the glucose assimilation pathway is illustrated in the figure below.