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Alvinella pompejana

cDNA library

Project: Collaboratif
State of the project: En cours



Collaborations :
Laboratoire de Biologie et Génomique Intégratives IGBMC
Illkirch, France Centre d’Etudes d’Océanographie et de Biologie Marine Station Biologique Roscoff, France

Within the framework of this project, three cDNA libraries will be produced at Genoscope :

  • a library from the whole animal,
  • a second one from the gills
  • and a third from the ventral tissue of the animal, which has the property of being free of bacteria.

The cDNAs obtained from RNAs by the “oligo-capping” method will be cloned in the plasmid vector pMe18S-f13.

About 50,000 clones from each library will be sequenced at the 5’ end, and then the clones which contain complete ORFs will be rearranged and sequenced from the 3’ end.

Contacts:
Patrick Wincker (Genoscope) - Olivier Poch (IGBMC)

Last update on 15 January 2008

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