Hemiascomycetes

The identification of molecular evolutionary mechanisms in eukaryotes is approached by a comparative genomics study of a homogeneous group of 13 species classified as Hemiascomycetes. Detailed information is provided by the bioinformatic analysis of 50 Mb of genomic sequences. All data are available in the 21 articles published in the FEBS Letters special issue n° 487, issue n°1, december 22, 2000. Major conclusions:

• identification of 20,000 new genes homologs of Saccharomyces cerevisiae;<
• analysis of Ascomycetes specific genes;
• analysis of gene redundancy in the Hemiascomycetes class;
• comparative analysis of chromosomal maps and conservation of synteny versus Saccharomyces cerevisiae;
• detailed analysis of the 13 Hemiascomycetes species.

Genomic DNA Sequences as protein alignments used for comparative studies are available on the URL site Génolevures.
A link to the CLIB’s site (the yeast collection for Biotechnology of INRA).

The selected species are (with the number of STS) [ and the prefix for sequence identification]:

• Candida tropicalis (2500) [BD]
• Debaromyces hansenii (2500) [BC]
• Hansenula polymorpha (5000) [BB]
• Kluyveromyces lactis (5000) [BA]
• Kluyveromyces marxianus var. marxianus (2500) [AZ]
• Kluyveromyces thermotolerans (2500) [AY]
• Pichia sorbitophila (5000) [AX]
• Saccharomyces exiguus (2500) [AV]
• Saccharomyces kluyveri (2500) [AU]
• Saccharomyces sevazzi (2500) [AT]
• Saccharomyces uvarum (5000) [AS]
• Yarrowia lipolytica (5000) [AW]
• Zygosaccharomyces rouxii (5000) [AR]

This is a collaborative project between :

• Génétique et Microbiologie UPRES-A 7010 ULP/CNRS, Strasbourg
• Laboratoire de Génétique Moléculaire, IGM, Orsay
• Centre de Génétique Moléculaire et Cellulaire, UMR 5534 CNRS, Université Claude Bernard, Lyon
• Unité de Génétique Moléculaire des Levures, Département des Biotechnologies, Institut Pasteur
• Génétique Moléculaire et Cellulaire INRA-CNRS, Grignon
• Laboratoire de Biologie Cellulaire de la levure, IBGC CNRS, Bordeaux