BLASTP 2.12.0+
Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics: Alejandro A. Schaffer,
L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri
I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids
Res. 29:2994-3005.
Database: d0e3f0408600160beb9c8e9d771c8ef9.TrEMBL.fasta
18 sequences; 7,678 total letters
Query= ACIAD3431
Length=449
Score E
Sequences producing significant alignments: (Bits) Value
A0A7G2SGF0 Type I restriction endonuclease subunit S [Acinetobact... 883 0.0
Q6F778 Putative type I restriction-modification system specificit... 883 0.0
N8U6C4 Type I restriction modification DNA specificity domain-con... 581 0.0
N8W0G5 Type I restriction modification DNA specificity domain-con... 581 0.0
A0A4R0FM54 Restriction endonuclease subunit S [Acinetobacter sp. ... 551 0.0
A0A369ZCS6 Restriction endonuclease subunit S [Haemophilus paraha... 388 2e-135
A0A261KUV0 Type I restriction modification DNA specificity domain... 341 9e-117
A0A1V3K353 Type I restriction modification DNA specificity domain... 340 3e-116
A0A1H9I768 Type I restriction enzyme, S subunit [Giesbergeria anu... 329 3e-112
A0A378IN04 Type I restriction-modification system (Methylase_S) [... 327 2e-111
A9YUI0 Probable type I restriction-modification system specificit... 136 7e-39
Q8GAY0 ORF5 protein [Staphylococcus aureus] 128 4e-36
Q47280 HsdS protein [Escherichia coli] 82.0 4e-20
O68172 HsdS subunit [Lactococcus lactis] 58.5 2e-12
Q8KLM8 Restriction-modification enzyme type I S subunit [Streptoc... 56.2 1e-11
O54676 Type I restriction modification DNA specificity domain-con... 50.8 4e-10
D7EZK5 FclI S subunit [Flavobacterium columnare] 48.1 4e-09
O52274 Specificity subunit [Lactococcus lactis subsp. lactis bv. ... 42.4 3e-07
>A0A7G2SGF0 Type I restriction endonuclease subunit S [Acinetobacter
sp]
Length=448
Score = 883 bits (2282), Expect = 0.0
Identities = 448/448 (100%), Positives = 448/448 (100%), Gaps = 0/448 (0%)
Query 1 MSQLPCYESYKNSGVQWLGEIPSHWEVKRMKFLLSEKLKYGANESAESEDKDQPRYIRIT 60
MSQLPCYESYKNSGVQWLGEIPSHWEVKRMKFLLSEKLKYGANESAESEDKDQPRYIRIT
Sbjct 1 MSQLPCYESYKNSGVQWLGEIPSHWEVKRMKFLLSEKLKYGANESAESEDKDQPRYIRIT 60
Query 61 DINDSGTLREDTFKSLEIEKAQEYLLNDLDILLARSGATVGKSYLHKKDKVNVACYAGYL 120
DINDSGTLREDTFKSLEIEKAQEYLLNDLDILLARSGATVGKSYLHKKDKVNVACYAGYL
Sbjct 61 DINDSGTLREDTFKSLEIEKAQEYLLNDLDILLARSGATVGKSYLHKKDKVNVACYAGYL 120
Query 121 IRARFNKENYDPQFINLFLQSKAYWSWIESVNIQATIQNVSAEKYNDLALSIPSLAEQKI 180
IRARFNKENYDPQFINLFLQSKAYWSWIESVNIQATIQNVSAEKYNDLALSIPSLAEQKI
Sbjct 121 IRARFNKENYDPQFINLFLQSKAYWSWIESVNIQATIQNVSAEKYNDLALSIPSLAEQKI 180
Query 181 IADFLDKRLAQVDALIAKQETLLEKLAEQRVALISHAVTKGLNPDVEMKESDVVLLGNIP 240
IADFLDKRLAQVDALIAKQETLLEKLAEQRVALISHAVTKGLNPDVEMKESDVVLLGNIP
Sbjct 181 IADFLDKRLAQVDALIAKQETLLEKLAEQRVALISHAVTKGLNPDVEMKESDVVLLGNIP 240
Query 241 NTWNIKRLKFLLSEKLKYGANESAESEDKENPRYIRITDIDDSGNLKDETFKSLESEKAQ 300
NTWNIKRLKFLLSEKLKYGANESAESEDKENPRYIRITDIDDSGNLKDETFKSLESEKAQ
Sbjct 241 NTWNIKRLKFLLSEKLKYGANESAESEDKENPRYIRITDIDDSGNLKDETFKSLESEKAQ 300
Query 301 EYLLDDLDILLARSGATVGKSYLYKAESVGIACYAGYLIRARLDQENYNPEFVNYFLQSK 360
EYLLDDLDILLARSGATVGKSYLYKAESVGIACYAGYLIRARLDQENYNPEFVNYFLQSK
Sbjct 301 EYLLDDLDILLARSGATVGKSYLYKAESVGIACYAGYLIRARLDQENYNPEFVNYFLQSK 360
Query 361 QYWDWISSINIQATIQNVSAEKYNDLTLAIPSLEEQKQLIEYLKNEDEKFNRAISKGKKL 420
QYWDWISSINIQATIQNVSAEKYNDLTLAIPSLEEQKQLIEYLKNEDEKFNRAISKGKKL
Sbjct 361 QYWDWISSINIQATIQNVSAEKYNDLTLAIPSLEEQKQLIEYLKNEDEKFNRAISKGKKL 420
Query 421 VHLLNEYRSTLITQVVTGKIDVQNLKVN 448
VHLLNEYRSTLITQVVTGKIDVQNLKVN
Sbjct 421 VHLLNEYRSTLITQVVTGKIDVQNLKVN 448
>Q6F778 Putative type I restriction-modification system specificity
determinant for hsdM and hsdR (HsdS) [Acinetobacter baylyi
(strain ATCC 33305 / BD413 / ADP1)]
Length=448
Score = 883 bits (2282), Expect = 0.0
Identities = 448/448 (100%), Positives = 448/448 (100%), Gaps = 0/448 (0%)
Query 1 MSQLPCYESYKNSGVQWLGEIPSHWEVKRMKFLLSEKLKYGANESAESEDKDQPRYIRIT 60
MSQLPCYESYKNSGVQWLGEIPSHWEVKRMKFLLSEKLKYGANESAESEDKDQPRYIRIT
Sbjct 1 MSQLPCYESYKNSGVQWLGEIPSHWEVKRMKFLLSEKLKYGANESAESEDKDQPRYIRIT 60
Query 61 DINDSGTLREDTFKSLEIEKAQEYLLNDLDILLARSGATVGKSYLHKKDKVNVACYAGYL 120
DINDSGTLREDTFKSLEIEKAQEYLLNDLDILLARSGATVGKSYLHKKDKVNVACYAGYL
Sbjct 61 DINDSGTLREDTFKSLEIEKAQEYLLNDLDILLARSGATVGKSYLHKKDKVNVACYAGYL 120
Query 121 IRARFNKENYDPQFINLFLQSKAYWSWIESVNIQATIQNVSAEKYNDLALSIPSLAEQKI 180
IRARFNKENYDPQFINLFLQSKAYWSWIESVNIQATIQNVSAEKYNDLALSIPSLAEQKI
Sbjct 121 IRARFNKENYDPQFINLFLQSKAYWSWIESVNIQATIQNVSAEKYNDLALSIPSLAEQKI 180
Query 181 IADFLDKRLAQVDALIAKQETLLEKLAEQRVALISHAVTKGLNPDVEMKESDVVLLGNIP 240
IADFLDKRLAQVDALIAKQETLLEKLAEQRVALISHAVTKGLNPDVEMKESDVVLLGNIP
Sbjct 181 IADFLDKRLAQVDALIAKQETLLEKLAEQRVALISHAVTKGLNPDVEMKESDVVLLGNIP 240
Query 241 NTWNIKRLKFLLSEKLKYGANESAESEDKENPRYIRITDIDDSGNLKDETFKSLESEKAQ 300
NTWNIKRLKFLLSEKLKYGANESAESEDKENPRYIRITDIDDSGNLKDETFKSLESEKAQ
Sbjct 241 NTWNIKRLKFLLSEKLKYGANESAESEDKENPRYIRITDIDDSGNLKDETFKSLESEKAQ 300
Query 301 EYLLDDLDILLARSGATVGKSYLYKAESVGIACYAGYLIRARLDQENYNPEFVNYFLQSK 360
EYLLDDLDILLARSGATVGKSYLYKAESVGIACYAGYLIRARLDQENYNPEFVNYFLQSK
Sbjct 301 EYLLDDLDILLARSGATVGKSYLYKAESVGIACYAGYLIRARLDQENYNPEFVNYFLQSK 360
Query 361 QYWDWISSINIQATIQNVSAEKYNDLTLAIPSLEEQKQLIEYLKNEDEKFNRAISKGKKL 420
QYWDWISSINIQATIQNVSAEKYNDLTLAIPSLEEQKQLIEYLKNEDEKFNRAISKGKKL
Sbjct 361 QYWDWISSINIQATIQNVSAEKYNDLTLAIPSLEEQKQLIEYLKNEDEKFNRAISKGKKL 420
Query 421 VHLLNEYRSTLITQVVTGKIDVQNLKVN 448
VHLLNEYRSTLITQVVTGKIDVQNLKVN
Sbjct 421 VHLLNEYRSTLITQVVTGKIDVQNLKVN 448
>N8U6C4 Type I restriction modification DNA specificity domain-containing
protein [Acinetobacter sp. CIP 102159]
Length=435
Score = 581 bits (1498), Expect = 0.0
Identities = 315/449 (70%), Positives = 356/449 (79%), Gaps = 15/449 (3%)
Query 1 MSQLPCYESYKNSGVQWLGEIPSHWEVKRMKFLLSEKLKYGANESAESEDKDQPRYIRIT 60
MSQL YESYK+SGVQWLGEIPSHW+VKRMKF+++ + ++++ D RY +
Sbjct 1 MSQLSRYESYKDSGVQWLGEIPSHWDVKRMKFVVAN-----VGDKIDAKESDL-RYFGLE 54
Query 61 DIND-SGTLREDTFKSLEIEKAQEYLLNDLDILLARSGATVGKSYLHKKDKVNVACYAGY 119
+I +G L + LE E D D+L + + K +L +++ ++
Sbjct 55 NIESFTGKLLDSV--ELESEGIGHRFQKD-DVLFGKLRPYLAKVFLAQEE--GLSSTEAL 109
Query 120 LIRARFNKENYDPQFINLFLQSKAYWSWIESVNIQATIQNVSAEKYNDLALSIPSLAEQK 179
+ R+ E DP+F++ + S+ + + + + + S + + + PSL EQK
Sbjct 110 VFRS---SEVIDPKFLSYYCLSQDFINEVNGTTFGSKMPRASWDDISSFRMVYPSLDEQK 166
Query 180 IIADFLDKRLAQVDALIAKQETLLEKLAEQRVALISHAVTKGLNPDVEMKESDVVLLGNI 239
IADFLDKRLAQVDALIAKQETLLEKLAEQRVALISHAVTKGLNPDVEMKESDVVLLGNI
Sbjct 167 TIADFLDKRLAQVDALIAKQETLLEKLAEQRVALISHAVTKGLNPDVEMKESDVVLLGNI 226
Query 240 PNTWNIKRLKFLLSEKLKYGANESAESEDKENPRYIRITDIDDSGNLKDETFKSLESEKA 299
PNTWN+KRLKFLLSEKLKYGANESAESEDKENPRYIRITDIDDSGNLKDETFKSLESEKA
Sbjct 227 PNTWNVKRLKFLLSEKLKYGANESAESEDKENPRYIRITDIDDSGNLKDETFKSLESEKA 286
Query 300 QEYLLDDLDILLARSGATVGKSYLYKAESVGIACYAGYLIRARLDQENYNPEFVNYFLQS 359
QEYLLDDLDILLARSGATVGKSYLYKAESVGIACYAGYLIRARLDQENYNPEFVNYFLQS
Sbjct 287 QEYLLDDLDILLARSGATVGKSYLYKAESVGIACYAGYLIRARLDQENYNPEFVNYFLQS 346
Query 360 KQYWDWISSINIQATIQNVSAEKYNDLTLAIPSLEEQKQLIEYLKNEDEKFNRAISKGKK 419
KQYWDWISSINIQATIQNVSAEKYNDL LAIP LEEQKQLIEYLKNEDEKFN AI KG+K
Sbjct 347 KQYWDWISSINIQATIQNVSAEKYNDLPLAIPPLEEQKQLIEYLKNEDEKFNSAIGKGQK 406
Query 420 LVHLLNEYRSTLITQVVTGKIDVQNLKVN 448
LV LLNEYRSTLITQVVTGKIDV+NLKVN
Sbjct 407 LVQLLNEYRSTLITQVVTGKIDVRNLKVN 435
>N8W0G5 Type I restriction modification DNA specificity domain-containing
protein [Acinetobacter sp. CIP 102529]
Length=435
Score = 581 bits (1498), Expect = 0.0
Identities = 315/449 (70%), Positives = 356/449 (79%), Gaps = 15/449 (3%)
Query 1 MSQLPCYESYKNSGVQWLGEIPSHWEVKRMKFLLSEKLKYGANESAESEDKDQPRYIRIT 60
MSQL YESYK+SGVQWLGEIPSHW+VKRMKF+++ + ++++ D RY +
Sbjct 1 MSQLSRYESYKDSGVQWLGEIPSHWDVKRMKFVVAN-----VGDKIDAKESDL-RYFGLE 54
Query 61 DIND-SGTLREDTFKSLEIEKAQEYLLNDLDILLARSGATVGKSYLHKKDKVNVACYAGY 119
+I +G L + LE E D D+L + + K +L +++ ++
Sbjct 55 NIESFTGKLLDSV--ELESEGIGHRFQKD-DVLFGKLRPYLAKVFLAQEE--GLSSTEAL 109
Query 120 LIRARFNKENYDPQFINLFLQSKAYWSWIESVNIQATIQNVSAEKYNDLALSIPSLAEQK 179
+ R+ E DP+F++ + S+ + + + + + S + + + PSL EQK
Sbjct 110 VFRS---SEVIDPKFLSYYCLSQDFINEVNGTTFGSKMPRASWDDISSFRMVYPSLDEQK 166
Query 180 IIADFLDKRLAQVDALIAKQETLLEKLAEQRVALISHAVTKGLNPDVEMKESDVVLLGNI 239
IADFLDKRLAQVDALIAKQETLLEKLAEQRVALISHAVTKGLNPDVEMKESDVVLLGNI
Sbjct 167 TIADFLDKRLAQVDALIAKQETLLEKLAEQRVALISHAVTKGLNPDVEMKESDVVLLGNI 226
Query 240 PNTWNIKRLKFLLSEKLKYGANESAESEDKENPRYIRITDIDDSGNLKDETFKSLESEKA 299
PNTWN+KRLKFLLSEKLKYGANESAESEDKENPRYIRITDIDDSGNLKDETFKSLESEKA
Sbjct 227 PNTWNVKRLKFLLSEKLKYGANESAESEDKENPRYIRITDIDDSGNLKDETFKSLESEKA 286
Query 300 QEYLLDDLDILLARSGATVGKSYLYKAESVGIACYAGYLIRARLDQENYNPEFVNYFLQS 359
QEYLLDDLDILLARSGATVGKSYLYKAESVGIACYAGYLIRARLDQENYNPEFVNYFLQS
Sbjct 287 QEYLLDDLDILLARSGATVGKSYLYKAESVGIACYAGYLIRARLDQENYNPEFVNYFLQS 346
Query 360 KQYWDWISSINIQATIQNVSAEKYNDLTLAIPSLEEQKQLIEYLKNEDEKFNRAISKGKK 419
KQYWDWISSINIQATIQNVSAEKYNDL LAIP LEEQKQLIEYLKNEDEKFN AI KG+K
Sbjct 347 KQYWDWISSINIQATIQNVSAEKYNDLPLAIPPLEEQKQLIEYLKNEDEKFNSAIGKGQK 406
Query 420 LVHLLNEYRSTLITQVVTGKIDVQNLKVN 448
LV LLNEYRSTLITQVVTGKIDV+NLKVN
Sbjct 407 LVQLLNEYRSTLITQVVTGKIDVRNLKVN 435
>A0A4R0FM54 Restriction endonuclease subunit S [Acinetobacter
sp. ANC 4173]
Length=442
Score = 551 bits (1420), Expect = 0.0
Identities = 302/452 (67%), Positives = 348/452 (77%), Gaps = 14/452 (3%)
Query 1 MSQLPCYESYKNSGVQWLGEIPSHWE---VKRMKFLLSEKLKYGANESAESEDKDQPRYI 57
MSQLP YESYK+SGVQWLGEIPSHW+ +KR+ + + +D+ + ++
Sbjct 1 MSQLPRYESYKDSGVQWLGEIPSHWKCLSIKRLTLVKRGASPRPIDNPEYFDDEGEYAWV 60
Query 58 RITDINDSGT-LREDTFKSLEIEKAQEYLLNDLDILLARSGATVGKSYLHKKDKVNVACY 116
RI D+ S L E T + E+ + + I L+ +G+ VGK + KV +
Sbjct 61 RIADVTASDKYLLETTQRLSELGNSLSVKMQPDSIFLSIAGS-VGKPII---TKVKACIH 116
Query 117 AGYLIRARFNKENYDPQFINLFLQSKAYWSWIESVNIQATIQNVSAEKYNDLALSIPSLA 176
G++ + KE+ + + +F + Y + T N++ + ++ +++P
Sbjct 117 DGFVYFPDW-KESINFLYY-IFASGQPYLG----LGKLGTQLNLNTDTVGNIKIALPPSD 170
Query 177 EQKIIADFLDKRLAQVDALIAKQETLLEKLAEQRVALISHAVTKGLNPDVEMKESDVVLL 236
EQ+ IADFLDKRLAQVD+LI KQ TLLEKLAEQRVALISHAVTKGLNPDVEMKES + +L
Sbjct 171 EQEAIADFLDKRLAQVDSLITKQATLLEKLAEQRVALISHAVTKGLNPDVEMKESGIEIL 230
Query 237 GNIPNTWNIKRLKFLLSEKLKYGANESAESEDKENPRYIRITDIDDSGNLKDETFKSLES 296
GNIP W IKRLKFLLSEKLKYGANESAESEDKENPRYIRITDIDDSGNLKDETFKSLE
Sbjct 231 GNIPQQWKIKRLKFLLSEKLKYGANESAESEDKENPRYIRITDIDDSGNLKDETFKSLEF 290
Query 297 EKAQEYLLDDLDILLARSGATVGKSYLYKAESVGIACYAGYLIRARLDQENYNPEFVNYF 356
EKA+EYLLDDLDILLARSGATVGKSYLYKAES+GIACYAGYLIRARLDQENYNPEFVNYF
Sbjct 291 EKAEEYLLDDLDILLARSGATVGKSYLYKAESIGIACYAGYLIRARLDQENYNPEFVNYF 350
Query 357 LQSKQYWDWISSINIQATIQNVSAEKYNDLTLAIPSLEEQKQLIEYLKNEDEKFNRAISK 416
LQSKQYWDWISSINIQATIQNVSAEKYNDLTLAIP LEEQKQLIEYLKNEDEKFN AI K
Sbjct 351 LQSKQYWDWISSINIQATIQNVSAEKYNDLTLAIPPLEEQKQLIEYLKNEDEKFNSAIGK 410
Query 417 GKKLVHLLNEYRSTLITQVVTGKIDVQNLKVN 448
GKKLVHLLNEYRSTLITQVVTGKIDV+NLK+N
Sbjct 411 GKKLVHLLNEYRSTLITQVVTGKIDVRNLKLN 442
>A0A369ZCS6 Restriction endonuclease subunit S [Haemophilus parahaemolyticus]
Length=439
Score = 388 bits (997), Expect = 2e-135
Identities = 219/447 (49%), Positives = 285/447 (64%), Gaps = 25/447 (6%)
Query 7 YESYKNSGVQWLGEIPSHWEVKRMKFLLSEKLKYGANESAESEDKDQPRYIRIT--DIND 64
YE YK+SG+ WLGE+P HW+ +K++ ++ G +++ + + Y +T DI++
Sbjct 4 YERYKDSGIPWLGEVPEHWDTMSLKYICD--VRDGTHDTPQYIYESDNSYPLVTSKDISN 61
Query 65 SGTLREDTFKSLEIEKAQEYL-------LNDLDILLARSGATVGKSYLHKKDKVNVACYA 117
F+S + ++YL +N D+L+ G TVG S L V Y
Sbjct 62 GNI----NFESAKFISEEDYLNISKRSNVNFGDLLMPMIG-TVGGSVL-------VETYR 109
Query 118 GYLIR--ARFNKENYDPQFINLFLQSKAYWSWIESVNIQATIQNVSAEKYNDLALSIPSL 175
+ I+ A F + +++ FL S+ E V +L + L
Sbjct 110 KFTIKNVALFKAGQFCAKWLKYFLDSELSKIQFELEKNGGVQDFVGLTTLRNLIILKLPL 169
Query 176 AEQKIIADFLDKRLAQVDALIAKQETLLEKLAEQRVALISHAVTKGLNPDVEMKESDVVL 235
EQ IA +LD +L ++DALI KQ+TL+EKLAEQR A+I+HAVTKGLNP MK+S V
Sbjct 170 KEQTTIAHYLDTKLGEIDALIDKQQTLIEKLAEQRTAVITHAVTKGLNPAAPMKKSGVEW 229
Query 236 LGNIPNTWNIKRLKFLLSEKLKYGANESAESEDKENPRYIRITDIDDSGNLKDETFKSLE 295
LG++P W++KRLKF+LSEKLKYGANESAES+D E PRYIRITDID+ GNLKD+TFKSLE
Sbjct 230 LGDVPAHWDVKRLKFMLSEKLKYGANESAESDDPEQPRYIRITDIDEQGNLKDDTFKSLE 289
Query 296 SEKAQEYLLDDLDILLARSGATVGKSYLYKAESVGIACYAGYLIRARLDQENYNPEFVNY 355
EKA++YLL+ DILLARSGATVGKSYLYK ACYAGYLIRARL+Q+ +N FVNY
Sbjct 290 FEKAKDYLLNVNDILLARSGATVGKSYLYKENLKNFACYAGYLIRARLEQKKFNANFVNY 349
Query 356 FLQSKQYWDWISSINIQATIQNVSAEKYNDLTLAIPSLEEQKQLIEYLKNEDEKFNRAIS 415
+ QS YWDWI S+NIQATIQNVSAEKYND L+IP L+EQ + +YL E K +R
Sbjct 350 YFQSTSYWDWIKSVNIQATIQNVSAEKYNDFLLSIPDLQEQNSITDYLDQETAKIDRLCE 409
Query 416 KGKKLVHLLNEYRSTLITQVVTGKIDV 442
+ + L EYR+ LITQ VTGKI V
Sbjct 410 TVNQTIGRLKEYRTVLITQAVTGKIKV 436
>A0A261KUV0 Type I restriction modification DNA specificity domain-containing
protein [Hydrocoleum sp. CS-953]
Length=453
Score = 341 bits (874), Expect = 9e-117
Identities = 192/446 (43%), Positives = 287/446 (64%), Gaps = 18/446 (4%)
Query 7 YESYKNSGVQWLGEIPSHWEVKRMKFLLSEKLKYGANESAESEDKDQPRYIRITDINDSG 66
Y YK+SG++WLG+IP HWEVK++K +++ K G D+ + RITDI+D G
Sbjct 9 YPVYKSSGIEWLGDIPEHWEVKKIK-RITQTHKQGYYTENPYIDEGV-KLARITDIDDFG 66
Query 67 TLREDTFKSLEIEKAQEYL--LNDLDILLARSGATVGK----SYLHKKDKVNVACYAGYL 120
+ + ++I E L L D L ARSG T+G+ SYL + + +A YL
Sbjct 67 NVSFENMPFVQISDKDEKLFRLKKGDFLFARSG-TIGRFGLVSYLER------SVFASYL 119
Query 121 IRARFNKENYDPQFINLFLQSKAYWSWIESVNIQATIQNVSAEKYNDLALSIPSLAEQKI 180
IR RF ++ P+F+ S + + S +NV AE + L IP ++EQ+
Sbjct 120 IRFRF--KHVKPEFLKFAFSSSFFKESLISTLHGGANKNVHAENIKEQILVIPPISEQQT 177
Query 181 IADFLDKRLAQVDALIAKQETLLEKLAEQRVALISHAVTKGLNPDVEMKESDVVLLGNIP 240
IA+FLD++LA++D +AK++ ++E L EQ+ +I+ AVTKGLN DV +K S + LG IP
Sbjct 178 IANFLDEKLARIDEYLAKKQRIIELLKEQKTVIINQAVTKGLNLDVPIKYSGIEWLGEIP 237
Query 241 NTWNIKRLKFLLSEKLKYGANESAESEDKENPRYIRITDIDDSGNLKDETFKSLESEKAQ 300
W K+LK+L+ KLKYGANE A+S DK PRYIRITD D G L+DETF+SLE A+
Sbjct 238 EHWEAKKLKYLVEGKLKYGANEEAKSADKSQPRYIRITDFDGDGKLRDETFRSLEINLAK 297
Query 301 EYLLDDLDILLARSGATVGKSYLYKAESVGIACYAGYLIRARLDQENYNPEFVNYFLQSK 360
+YLL + DIL ARSGATVGK++ +K S G+AC+AGYLI+A + +++ F +S+
Sbjct 298 DYLLSEGDILFARSGATVGKTFQFKDYS-GLACFAGYLIKAIPKESIILSDYLYLFTKSE 356
Query 361 QYWDWISSINIQATIQNVSAEKYNDLTLAIPSLEEQKQLIEYLKNEDEKFNRAISKGKKL 420
Y W +SI IQATIQN+ A+KY+ L++++P + EQK+++ +++ + EK R+++ +K
Sbjct 357 VYDIWKTSIFIQATIQNIGADKYSCLSVSLPPISEQKEIVSFIETKSEKIERSLATIEKE 416
Query 421 VHLLNEYRSTLITQVVTGKIDVQNLK 446
+ L+ EYR+TLI++ VTGKIDV+ +
Sbjct 417 IKLIQEYRTTLISETVTGKIDVRKYR 442
>A0A1V3K353 Type I restriction modification DNA specificity domain-containing
protein [Rodentibacter pneumotropicus]
Length=477
Score = 340 bits (873), Expect = 3e-116
Identities = 208/481 (43%), Positives = 284/481 (59%), Gaps = 49/481 (10%)
Query 7 YESYKNSGVQWLGEIPSHWEVKRMKFLLSEKLKYGANESAESEDKDQPRYIRITDINDSG 66
YE YK SGV WLG++P HWEVKR+K+LL+EKLKYGANESAE +DKD PRYIRITDI + G
Sbjct 4 YEQYKPSGVAWLGDVPEHWEVKRLKYLLAEKLKYGANESAELDDKDLPRYIRITDIREDG 63
Query 67 TLREDTFKSLEIEKAQEYLLNDLDILLARSGATVGKSYLHKKDKVNVACYAGYLIRARFN 126
L+E+TFKSLE EKA+EYLL++ DILLARSGATVGKSYL+KK V VACYAGYLIRARF
Sbjct 64 KLKEETFKSLEYEKAEEYLLDNFDILLARSGATVGKSYLYKKSLVGVACYAGYLIRARFK 123
Query 127 KENYDPQFINLFLQSKAYWSWIESVNIQATIQNVSAEKYNDLALSIPSLAEQKIIADFLD 186
+E +P+FIN QS Y +W+++VNIQATIQNVSAEKYN+ L++P L EQ IA +LD
Sbjct 124 QEISNPEFINYVFQSNFYSAWVKAVNIQATIQNVSAEKYNEFTLALPPLEEQIAIATYLD 183
Query 187 KRLAQVDALIAKQETLLEKLAEQRVALISHAVTKG--LNPDVEMKESDVVLLGN------ 238
+R A +D LIAKQ+ L EKL+E+R ALI+ AV ++PD+ +++ +VL GN
Sbjct 184 ERTAYLDRLIAKQQALSEKLSEKRTALITEAVCGRVKISPDLCLQKKGIVLSGNQITEQK 243
Query 239 ----------------------------IPNTWNIKRLKFLLSEKLKYGANESAESEDKE 270
IP W +KRLKF+ + G ++
Sbjct 244 SIKKSLLFSKEGLGEIGFKDSGIQWLGKIPEHWEVKRLKFVGELQGGIGFPHDYQNIQDL 303
Query 271 NPRYIRITDIDDSGNLKD-----ETFKSLESEKAQEYLLDDLDILLARSGATVGKSYLYK 325
+ ++ D+ S N +D T ++K ++ + IL A+ GA + L +
Sbjct 304 EFGFYKVGDLGLSENNRDMPKAQHTVSIEIADKLGAKIIPENSILYAKVGAAL---LLNR 360
Query 326 AESVGIACYAGYLIRARL-DQENYNPEFVNYFLQSKQYWDWISSINIQATIQNVSAEKYN 384
G C + A + D + P + Y L + ++ I ++S
Sbjct 361 RRITGEKCCIDNNMTAFIPDVKQILPIWAFYTLSIIDFKILVNP----GAIPSLSEGYQK 416
Query 385 DLTLAIPSLEEQKQLIEYLKNEDEKFNRAISKGKKLVHLLNEYRSTLITQVVTGKIDVQN 444
+L++ +P+LEEQ + +L E K + +K + + L EYRS LITQVVTGKI VQ
Sbjct 417 NLSIPMPNLEEQTAIAAFLDKETAKIDCLQAKIAESIARLKEYRSALITQVVTGKIKVQG 476
Query 445 L 445
+
Sbjct 477 V 477
>A0A1H9I768 Type I restriction enzyme, S subunit [Giesbergeria
anulus]
Length=456
Score = 329 bits (844), Expect = 3e-112
Identities = 199/456 (44%), Positives = 274/456 (60%), Gaps = 24/456 (5%)
Query 1 MSQLPCYESYKNSGVQWLGEIPSHWEVKRMKFLLSEKLKYGANESAESEDKDQPRYIRIT 60
M P Y YK + WL EIP HW+ KR+K + + N+ E D I+
Sbjct 1 MENYPAYRKYKQTRCAWLKEIPEHWDFKRLKNVATH------NDETLDERADPDLEIKYV 54
Query 61 DINDSGTLRE-DTFKSLEIEKAQEYL---LNDLDILLARSGATVGKSYLHKKDKV----- 111
DI+ + + +++ EK+ + D DI+++ TV ++YL V
Sbjct 55 DISSVSLVNGIEKIEAMPFEKSPSRARRKVKDGDIIVS----TV-RTYLKAIAPVCEPPE 109
Query 112 NVACYAGYLIRARFNKENYDPQFINLFLQSKAYWSWIESVNIQATIQNVSAEKYNDLALS 171
N+ G+ + KEN F LQS + + + ++ + ++A + +
Sbjct 110 NMVVSTGFAVVRP--KENLFSGFAGYLLQSNGFVGDVVANSVGVSYPAINASDLARIPVI 167
Query 172 IPSLAEQKIIADFLDKRLAQVDALIAKQETLLEKLAEQRVALISHAVTKGLNPDVEMKES 231
P L EQK IA FLD + AQ+DALIAK++ LL+KLAE+R ALISHAVTKGL+ V MK+S
Sbjct 168 EPPLEEQKAIARFLDFKTAQIDALIAKKKALLDKLAEKRTALISHAVTKGLDSSVPMKDS 227
Query 232 DVVLLGNIPNTWNIKRLKFLLSEKLKYGANESAESEDKENPRYIRITDIDDSGNLKDETF 291
LG+IP W KRLKF++SE LKYGANE+AE D E PRYIRITD++D G+L+++TF
Sbjct 228 KAAWLGHIPRHWEAKRLKFVISEPLKYGANEAAELTDPELPRYIRITDVNDDGSLREDTF 287
Query 292 KSLESEKAQEYLLDDLDILLARSGATVGKSYLYKAESVGIACYAGYLIRARLDQENYNPE 351
+SL E A +YLL D DILLARSGATVGKS++YK S G+A YAGYLIRAR+ + NP+
Sbjct 288 RSLPQEIADDYLLSDGDILLARSGATVGKSFIYK-PSWGMAAYAGYLIRARM-SDAMNPD 345
Query 352 FVNYFLQSKQYWDWISSINIQATIQNVSAEKYNDLTLAIPSLEEQKQLIEYLKNEDEKFN 411
F FLQS YW W+SS+ IQATIQNVSAEKY +L + +P + EQ ++ L + + +
Sbjct 346 FAYLFLQSSCYWQWLSSVFIQATIQNVSAEKYANLVIPVPPINEQLSIVSRLLVQLKSID 405
Query 412 RAISKGKKLVHLLNEYRSTLITQVVTGKIDVQNLKV 447
+ ++ L EYRS LIT VTGKIDV+ +V
Sbjct 406 VQRERVGLVIDRLIEYRSALITNAVTGKIDVRGFQV 441
>A0A378IN04 Type I restriction-modification system (Methylase_S)
[Legionella cincinnatiensis]
Length=456
Score = 327 bits (838), Expect = 2e-111
Identities = 200/445 (45%), Positives = 275/445 (62%), Gaps = 15/445 (3%)
Query 7 YESYKNSGVQWLGEIPSHWEVKRMKFLLSEKLKYGANESAESEDKDQPRYIRITDINDSG 66
+E+ K++ V W E P W +KR++F+L+ L +N+ +D +I + + ++G
Sbjct 6 HENEKHTNVAWFDETPVTWCIKRLRFVLTTNLNK-SNKLLRPDDIVS--FIPMEAVGENG 62
Query 67 TLREDTFKSL-EIEKAQEYLLNDLDILLARSGATV--GKSYLHK--KDKVNVACYAGYLI 121
LR D K + EI Y + D+++A+ K L K K+ V Y++
Sbjct 63 GLRLDIEKQVSEIGDGYTYF-EEHDVVVAKITPCFENAKGALAKGLKNGVGYGTTELYVL 121
Query 122 RARFNKENYDPQFINLFLQSKAYWSWIESVNIQATIQNVSAEKY-NDLALSIPSLAEQKI 180
R N +++ S + E+ A Q E + + A +IPSL EQK
Sbjct 122 RPF---TNITSEYLFYLTISYPFRKLGEAEMYGAGGQKRVPENFIKNFATAIPSLLEQKT 178
Query 181 IADFLDKRLAQVDALIAKQETLLEKLAEQRVALISHAVTKGLNPDVEMKESDVVLLGNIP 240
I DFLDK+ ++D LIAK+ L++ L E+R+A I+ AVTKGLN + MK+S + +G IP
Sbjct 179 ITDFLDKKTTEIDVLIAKKYALIKLLEEKRIAFITQAVTKGLNKNTLMKDSYIPYIGKIP 238
Query 241 NTWNIKRLKFLLSEKLKYGANESAESEDKENPRYIRITDIDDSGNLKDETFKSLESEKAQ 300
W +KR KFL+SE KYGANESAE +D+ PRYIRITDI D+G LK++TFKSL EKA
Sbjct 239 VHWGVKRFKFLVSEPFKYGANESAEDDDRSQPRYIRITDIKDNGLLKEDTFKSLSEEKAI 298
Query 301 EYLLDDLDILLARSGATVGKSYLYKAESVGIACYAGYLIRARLDQENYNPEFVNYFLQSK 360
YLL D DILLARSGATVGKS++Y+A +G ACYAGYLIRAR+ NPEF+ FL S
Sbjct 299 PYLLKDGDILLARSGATVGKSFIYRAH-LGRACYAGYLIRARI-HSMINPEFIFRFLNSN 356
Query 361 QYWDWISSINIQATIQNVSAEKYNDLTLAIPSLEEQKQLIEYLKNEDEKFNRAISKGKKL 420
YWDW+++I IQATIQNVSAEKY +L + P LEEQ++++ L N+ +K I K +
Sbjct 357 SYWDWLNNIFIQATIQNVSAEKYANLCIPTPPLEEQEKILNVLDNKLKKIEETIFKITEA 416
Query 421 VHLLNEYRSTLITQVVTGKIDVQNL 445
+ LNEYRSTLIT VTGKI+V +L
Sbjct 417 IGYLNEYRSTLITHAVTGKINVNSL 441
Score = 228 bits (582), Expect = 4e-73
Identities = 121/220 (55%), Positives = 157/220 (71%), Gaps = 2/220 (1%)
Query 11 KNSGVQWLGEIPSHWEVKRMKFLLSEKLKYGANESAESEDKDQPRYIRITDINDSGTLRE 70
K+S + ++G+IP HW VKR KFL+SE KYGANESAE +D+ QPRYIRITDI D+G L+E
Sbjct 227 KDSYIPYIGKIPVHWGVKRFKFLVSEPFKYGANESAEDDDRSQPRYIRITDIKDNGLLKE 286
Query 71 DTFKSLEIEKAQEYLLNDLDILLARSGATVGKSYLHKKDKVNVACYAGYLIRARFNKENY 130
DTFKSL EKA YLL D DILLARSGATVGKS++++ + ACYAGYLIRAR +
Sbjct 287 DTFKSLSEEKAIPYLLKDGDILLARSGATVGKSFIYRA-HLGRACYAGYLIRARIH-SMI 344
Query 131 DPQFINLFLQSKAYWSWIESVNIQATIQNVSAEKYNDLALSIPSLAEQKIIADFLDKRLA 190
+P+FI FL S +YW W+ ++ IQATIQNVSAEKY +L + P L EQ+ I + LD +L
Sbjct 345 NPEFIFRFLNSNSYWDWLNNIFIQATIQNVSAEKYANLCIPTPPLEEQEKILNVLDNKLK 404
Query 191 QVDALIAKQETLLEKLAEQRVALISHAVTKGLNPDVEMKE 230
+++ I K + L E R LI+HAVT +N + ++E
Sbjct 405 KIEETIFKITEAIGYLNEYRSTLITHAVTGKINVNSLLQE 444
>A9YUI0 Probable type I restriction-modification system specificity
protein [Haemophilus influenzae]
Length=433
Score = 136 bits (343), Expect = 7e-39
Identities = 125/458 (27%), Positives = 207/458 (45%), Gaps = 50/458 (11%)
Query 7 YESYKNSGVQWLGEIPSHWEVKRMKFLLSEKLKYGANESAESEDKDQPRYIRI------- 59
YE YK+SGV+WLGEIP+HWE ++ + + NE D + I
Sbjct 4 YERYKDSGVEWLGEIPTHWECVPLRSIFKFR-----NEKNNPIKTDNILSLSIANGVTEY 58
Query 60 TDINDSGTLREDTFKSLEIEKAQEYLLNDLDILLARSGATVGKSYLHKKDKVNVACYAGY 119
+D N G R+D S ++ + +LN +++++ G V K + ++ YA
Sbjct 59 SDENRGGNKRKDDLSSYKLAYPNDIVLNSMNVIVGAVG--VSKYF----GAISPVYYALS 112
Query 120 LIRARFNKENYDPQFINLFLQ-------SKAYWSWIESVNIQATIQNVSAEKYNDLALSI 172
L R N Y+ F N Q + E+ + +S + L I
Sbjct 113 LHNQRANLSYYESIFKNENFQRGLLRFGKGILIKFGENGKMNTIRMKISQDDLKKLYFPI 172
Query 173 PSLAEQKIIADFLDKRLAQVDALIAKQETLLEKLAEQRVALISHAVTKGLNPDVEMKESD 232
L EQ+ IA FLD + A++D + E + L E + LI +AVT+GLNPDV +K+S
Sbjct 173 SPLDEQQKIAQFLDDKTAKIDRAVELAEKQIALLKEHKQILIQNAVTRGLNPDVPLKDSG 232
Query 233 VVLLGNIPNTWNIKRLKFLLSEKLKYGANESAESEDKENPRYIRITDIDDS-GNL---KD 288
V +G +P W +L G N E++ ++N T + S G + +
Sbjct 233 VEWIGQVPEHW-----------ELTIGMNVFRENK-RDNKGMKENTVLSLSYGKIIIKPE 280
Query 289 ETFKSLESEKAQEY-LLDDLDILLARSGATVGKSYLYKAESVGIACYAGYLIRARLDQE- 346
E L E + Y +++ DI++ + ++ L G+A G + A L+ +
Sbjct 281 EKLFGLVPESFETYQIVEPNDIIIRCTDLQNDQTSL----RTGLAQDKGIITSAYLNLKV 336
Query 347 --NYNPEFVNYFLQSKQYWDWISSINIQATIQNVSAEKYNDLTLAIPSLEEQKQLIEYLK 404
NY+ +F++Y+L + + QN+S + L + SL EQ+Q+ +YL
Sbjct 337 INNYSAKFLHYYLHALDITKVLYKFG-SGLRQNLSFLDFKRLPIIDISLAEQQQIADYLD 395
Query 405 NEDEKFNRAISKGKKLVHLLNEYRSTLITQVVTGKIDV 442
+ K ++AI+ + L EY+S LI VVTGK+ V
Sbjct 396 KQTSKIDQAIALKTAHIEKLKEYKSVLINDVVTGKVRV 433
>Q8GAY0 ORF5 protein [Staphylococcus aureus]
Length=406
Score = 128 bits (322), Expect = 4e-36
Identities = 101/353 (29%), Positives = 178/353 (50%), Gaps = 28/353 (8%)
Query 82 QEYLLNDLDILLARSGATVGKSYLHKKDKVNV-ACYAGYLIRARFNKENYDPQFINLFLQ 140
Q + N DIL+ R+G T K NV + + +F+K YD F+ L
Sbjct 75 QSVIANKEDILMTRTGNT-------GKVVTNVFGAFHNNFFKIKFDKNLYDRLFLVEVLN 127
Query 141 SKAYWSWIESVNIQATIQNVSAEKYNDLALSIPSLAEQKIIADFLDKRLAQVDALIAKQE 200
S + I S+ +TI +++ + ++ S P L EQ+ I DF K +D I QE
Sbjct 128 SSKIQNKILSLAGSSTIPDLNHSDFYSISSSYPLLREQQKIGDFFSK----IDRQIELQE 183
Query 201 TLLEKLAEQRVALISHAVTKGLNPDVEMKESDVVLLGNIPNTWNIKRLKFLLSEKLKYGA 260
LE L +Q+ + K + ++ K+ + G W K+L + +E+ YG
Sbjct 184 QKLELLQQQKKGYMQ----KIFSQELRFKDEN----GEDYPDWKEKKLGDI-TEQSMYGI 234
Query 261 NESAESEDKENPRYIRITDIDDSGNLKDETFKSLESEKAQEYLLDDLDILLARSGATVGK 320
SA D +N YIRITDID+ + + E +Y L DIL AR+GA+ GK
Sbjct 235 GASATRFDSKNI-YIRITDIDEKSRKLNYQNLTTPDELNNKYKLKRNDILFARTGASTGK 293
Query 321 SYLYKAE-SVGIACYAGYLIRARLDQENYNPEFVNYFLQSKQYWDWISSINIQATIQNVS 379
SY++K E + +AG+LI+ +D++N NP F+ F + ++ W+ +++++ ++
Sbjct 294 SYIHKEEKDIYNYYFAGFLIKFEIDEQN-NPLFIYQFTLTSKFNKWVKVMSVRSGQPGIN 352
Query 380 AEKYNDLTLAIPSLEEQKQLIEYLKNEDEKFNRAISKGKKLVHLLNEYRSTLI 432
+E+Y L L +P+ EQ+++ ++L ++F+R I K+ + +L + + L+
Sbjct 353 SEEYAKLPLVLPNKLEQQKIAKFL----DRFDRQIELEKQKIEILQQQKKGLL 401
Score = 90.1 bits (222), Expect = 9e-23
Identities = 64/199 (32%), Positives = 113/199 (57%), Gaps = 12/199 (6%)
Query 19 GEIPSHWEVKRMKFLLSEKLKYGANESAESEDKDQPRYIRITDINDSGTLREDTFKSLEI 78
GE W+ K++ + +E+ YG SA D YIRITDI++ R+ +++L
Sbjct 212 GEDYPDWKEKKLGDI-TEQSMYGIGASATRFDSKNI-YIRITDIDEKS--RKLNYQNLTT 267
Query 79 --EKAQEYLLNDLDILLARSGATVGKSYLHKKDK-VNVACYAGYLIRARFNKENYDPQFI 135
E +Y L DIL AR+GA+ GKSY+HK++K + +AG+LI+ +++N +P FI
Sbjct 268 PDELNNKYKLKRNDILFARTGASTGKSYIHKEEKDIYNYYFAGFLIKFEIDEQN-NPLFI 326
Query 136 NLFLQSKAYWSWIESVNIQATIQNVSAEKYNDLALSIPSLAEQKIIADFLDKRLAQVDAL 195
F + + W++ +++++ +++E+Y L L +P+ EQ+ IA FLD+ D
Sbjct 327 YQFTLTSKFNKWVKVMSVRSGQPGINSEEYAKLPLVLPNKLEQQKIAKFLDR----FDRQ 382
Query 196 IAKQETLLEKLAEQRVALI 214
I ++ +E L +Q+ L+
Sbjct 383 IELEKQKIEILQQQKKGLL 401
>Q47280 HsdS protein [Escherichia coli]
Length=406
Score = 82.0 bits (201), Expect = 4e-20
Identities = 90/382 (24%), Positives = 169/382 (44%), Gaps = 62/382 (16%)
Query 42 ANESAESEDKDQPRYIRITDINDSGTLREDTFKSLEIEKAQEYLLNDLDILLARSGATVG 101
+N ESE+ P Y+R +I L+ DTF+ +++ I++ G +G
Sbjct 32 SNRQDESENGIYPFYVRSKNI-----LKSDTFE-----------FDEVAIVIPGEGG-IG 74
Query 102 KSYLHKKDKVNVACYAGYLIRARFNKENYDPQFINLFLQSKAYWSWIESVNIQATIQNVS 161
+ + + K + A R R D +F+ F+ S ++ +I + ++ AT ++
Sbjct 75 DIFHYVEGKYALHQRA---YRIRITTNAVDTKFLYYFMSS-SFKQYILTKSVGATAISIR 130
Query 162 AEKYNDLALSIPSLAEQKIIADFLDKRLAQVDALIAKQETLLEKLAEQRVALISHAVTKG 221
+ IPS D +K LA ++ +T AE +T
Sbjct 131 KPMLEGFKVPIPS-------PDNPEKSLAIQSEIVRILDTFTALTAE---------LTAE 174
Query 222 LNPDVEMKESDVVLLGNIPNTWNIKRLKF--------LLSEKLKYGANESAESEDKENPR 273
L ++ M++ N + + L F L E + +A++ D + R
Sbjct 175 LTAELNMRKKQY-------NYYRDQLLSFKEGEVEWKTLGEIGNFTYGYAAKAMDSGDAR 227
Query 274 YIRITDIDDSGNLKDETFKSLE-SEKAQEYLLDDLDILLARSGATVGKSYLYKAESVGIA 332
++RITDI+ G L E +E +E+ ++Y LD D+L+AR+GAT GK+ +++ + A
Sbjct 228 FVRITDINKDGKLSKENPMYVELNEENEKYTLDKNDLLMARTGATFGKTMIFEEDYP--A 285
Query 333 CYAGYLIRARLDQENYNPEFVNYFLQSKQYWDWISSINIQATIQNVSAEKYNDLTLAIP- 391
YAG+LI+ L++ N ++ +F QS +W+ + + +A + + IP
Sbjct 286 VYAGFLIKLNLNETIINAKYYWHFAQSDFFWEQANKLVSGGGQPQFNANALKQVRVPIPY 345
Query 392 ------SLEEQKQLIEYLKNED 407
SL+EQ ++++ L D
Sbjct 346 PSHPQKSLDEQGRIVDILDKFD 367
>O68172 HsdS subunit [Lactococcus lactis]
Length=425
Score = 58.5 bits (140), Expect = 2e-12
Identities = 66/313 (21%), Positives = 137/313 (44%), Gaps = 37/313 (12%)
Query 134 FINLFLQSKAYWSWIESVNIQATIQNVSAEKYNDLALSIP-SLAEQKIIADFLDKRLAQV 192
F+ + L+S ++ + +++ T + VS + L++++P + EQ+ I F +
Sbjct 137 FLAVLLKSPLVFNNLSALSSGGTAKGVSQKSLKGLSITVPLDIDEQQKIGSFFK----HL 192
Query 193 DALIAKQETLLEKLAEQRVALISHAVTKGLNPDVEMKESDVVLLGNIPNTWNIKRLKFLL 252
D IA + L+ L EQ+ + K E++ + W ++L +
Sbjct 193 DDTIALHQRKLDLLKEQKKGYLQKMFPKNGAKVPELR------FAGFADDWEERKLGDIA 246
Query 253 SEKLKYGANESAESEDKENPRYIRITDIDDSGNLKDETFKSLESEKAQEYLLDDLDILLA 312
L+ G + K +RI++I SG + + E +K +Y+L D +LA
Sbjct 247 P--LRGGYAFKSSKFRKTGVPIVRISNILSSGEVGGDFAYYDEQDKDDKYILPDKSAVLA 304
Query 313 RSGATVGK---------SYLYKAESVGIACYAGYLIRARLDQENYNPEFVNYFLQSKQYW 363
SGAT GK +Y+ + VG Y+ +Y F++ ++S+ +
Sbjct 305 MSGATTGKVSILSQTDYDKVYQNQRVGYFQSVDYI--------DYG--FISTIVRSELFM 354
Query 364 DWISSINIQATIQNVSAEKYNDLTLAIPSL-EEQKQLIEYLKNEDEKFNRAISKGKKLVH 422
+ S+ + NVS+++ + IP L +EQ+++ + K D+ I+ ++ +
Sbjct 355 MQLESVLVSGAQPNVSSKEIDSFNFMIPILVQEQQKIGSFFKQLDD----TIALHQRKLD 410
Query 423 LLNEYRSTLITQV 435
LL E + + ++
Sbjct 411 LLKEQKKGFLQKM 423
Score = 56.6 bits (135), Expect = 8e-12
Identities = 51/159 (32%), Positives = 78/159 (49%), Gaps = 14/159 (9%)
Query 57 IRITDINDSGTLREDTFKSLEIEKAQEYLLNDLDILLARSGATVGKSYLHKK---DKVNV 113
+RI++I SG + D E +K +Y+L D +LA SGAT GK + + DKV
Sbjct 267 VRISNILSSGEVGGDFAYYDEQDKDDKYILPDKSAVLAMSGATTGKVSILSQTDYDKVYQ 326
Query 114 ACYAGYLIRARFNKENY-DPQFINLFLQSKAYWSWIESVNIQATIQNVSAEKYNDLALSI 172
GY F +Y D FI+ ++S+ + +ESV + NVS+++ + I
Sbjct 327 NQRVGY-----FQSVDYIDYGFISTIVRSELFMMQLESVLVSGAQPNVSSKEIDSFNFMI 381
Query 173 PSLA-EQKIIADFLDKRLAQVDALIAKQETLLEKLAEQR 210
P L EQ+ I F Q+D IA + L+ L EQ+
Sbjct 382 PILVQEQQKIGSF----FKQLDDTIALHQRKLDLLKEQK 416
>Q8KLM8 Restriction-modification enzyme type I S subunit [Streptococcus
thermophilus]
Length=407
Score = 56.2 bits (134), Expect = 1e-11
Identities = 98/435 (23%), Positives = 185/435 (43%), Gaps = 70/435 (16%)
Query 25 WEVKRMKFLLSEKLKYGANESAE----SEDKDQPRYIRITDINDSGTLREDT-FKSLEIE 79
WE +++ L S+K+ G S+ S+D P +I+ DI ++ ++ + S E +
Sbjct 17 WEQRKLGEL-SQKISVGIATSSSKYFSSQDHGVP-FIKNQDIKENRINTKNLEYISKEFD 74
Query 80 -KAQEYLLNDLDILLARSGATVGKSYLHKKDKVNVACYAGYLIRARFNKENYDPQFINLF 138
K + + DI+ AR+G G S + K+ + + R E ++I++F
Sbjct 75 NKNKNKRVKQGDIITARTGYP-GLSAVVPKELEGAQTFTTLITRPI--SEMILSEYISIF 131
Query 139 LQSKAYWSWIESVNIQATIQNVSAEKYNDLALSIPSLAEQKIIADFLDKRLAQVDALIAK 198
+ S I + +NV+A +L + +PSL EQK I++F+ K +D IA
Sbjct 132 INSPYGMKQISGMEAGGAQKNVNAGIVQNLLIPLPSLDEQKKISNFILK----LDDTIAL 187
Query 199 QETLLEKLAEQRVALISHAVTKGLNPDVEMKESDVVLLGNIPNTWNIKRLKFLLSEKLKY 258
+ L+ L EQ+ + K E++ + W +++L
Sbjct 188 HQRKLDLLKEQKKGYLQKMFPKNGAKVPELR------FAGFADDWEVRKL---------- 231
Query 259 GANESAESED--KENPRYIRITDIDDSG--NLKDETFKSLESEK--AQEYLLDDL----- 307
NE ++ D + P+Y D+G L E K+L S K ++E D+
Sbjct 232 --NEVSDIYDGTHQTPKY------QDNGVMFLSVENIKTLTSNKFISREAFEDEFKIRPQ 283
Query 308 --DILLARSGATVGKSYLYKAESVGIACYAGYLIRARLDQENYNPEFVNYFLQSKQYWDW 365
D+L+ R G +G + + + + A Y+ A E NP YFLQ+ Y +
Sbjct 284 RGDVLMTRIG-DIGTANVVETDE----DLAYYVSLALFKSEELNP----YFLQASIYAPF 334
Query 366 ISSINIQATIQNVSAEKYN-----DLTLAIPSLEEQKQLIEYLKNEDEKFNRAISKGKKL 420
+ + T+ +K N + + +P+L EQ ++ + K D + I+ ++
Sbjct 335 VQDQIWKRTLHIAFPKKINKNEIGQVPINVPTLAEQTKIGSFFKQLD----KTIALHQRK 390
Query 421 VHLLNEYRSTLITQV 435
+ LL E + + ++
Sbjct 391 LDLLKEQKKGFLQKM 405
Score = 41.2 bits (95), Expect = 6e-07
Identities = 51/206 (25%), Positives = 95/206 (46%), Gaps = 15/206 (7%)
Query 229 KESDVVLLGNIPNTWNIKRLKFLLSEKLKYGANESAE----SEDKENPRYIRITDIDDSG 284
K+S + + W ++L L S+K+ G S+ S+D P +I+ DI ++
Sbjct 3 KKSPQLRFEGFTDDWEQRKLGEL-SQKISVGIATSSSKYFSSQDHGVP-FIKNQDIKENR 60
Query 285 -NLKDETFKSLESE-KAQEYLLDDLDILLARSGATVGKSYLYKAESVGIACYAGYLIRAR 342
N K+ + S E + K + + DI+ AR+G G S + E G + + R
Sbjct 61 INTKNLEYISKEFDNKNKNKRVKQGDIITARTGYP-GLSAVVPKELEGAQTFTTLITRPI 119
Query 343 LDQENYNPEFVNYFLQSKQYWDWISSINIQATIQNVSAEKYNDLTLAIPSLEEQKQLIEY 402
E E+++ F+ S IS + +NV+A +L + +PSL+EQK++ +
Sbjct 120 --SEMILSEYISIFINSPYGMKQISGMEAGGAQKNVNAGIVQNLLIPLPSLDEQKKISNF 177
Query 403 LKNEDEKFNRAISKGKKLVHLLNEYR 428
+ D+ I+ ++ + LL E +
Sbjct 178 ILKLDD----TIALHQRKLDLLKEQK 199
>O54676 Type I restriction modification DNA specificity domain-containing
protein [Lactococcus lactis subsp. cremoris]
Length=310
Score = 50.8 bits (120), Expect = 4e-10
Identities = 37/149 (25%), Positives = 70/149 (47%), Gaps = 9/149 (6%)
Query 62 INDSGTLREDTFKSLEIEKAQEYLLNDLDILLARSGATVGKSYLHKKDKVNVACYAGYLI 121
+N + ++T E ++++ L ++D +L T+G + ++V + A YL
Sbjct 61 VNGKIVITKETKLVTESNQSKDDKLLNMDTILMSINGTIGNLAWYNNERVMLGKSAAYL- 119
Query 122 RARFNKENYDPQFINLFLQSKAYWSWIESVNIQATIQNVSAEKYNDLALSIPSLAEQKII 181
N+D +FI +LQ+ ++ + TI+N+ + D L +P+L EQ+ I
Sbjct 120 ----TVSNFDKKFIFSYLQTSTIKNYFLNNLTGTTIKNLGLKTIRDTTLFVPTLEEQQKI 175
Query 182 ADFLDKRLAQVDALIAKQETLLEKLAEQR 210
F Q+D IA + L+ L EQ+
Sbjct 176 GSF----FKQLDDTIALHQRKLDLLKEQK 200
Score = 50.1 bits (118), Expect = 7e-10
Identities = 36/140 (26%), Positives = 70/140 (50%), Gaps = 9/140 (6%)
Query 289 ETFKSLESEKAQEYLLDDLDILLARSGATVGKSYLYKAESVGIACYAGYLIRARLDQENY 348
ET ES ++++ L ++D +L T+G Y E V + A YL + N+
Sbjct 70 ETKLVTESNQSKDDKLLNMDTILMSINGTIGNLAWYNNERVMLGKSAAYLTVS-----NF 124
Query 349 NPEFVNYFLQSKQYWDWISSINIQATIQNVSAEKYNDLTLAIPSLEEQKQLIEYLKNEDE 408
+ +F+ +LQ+ ++ + TI+N+ + D TL +P+LEEQ+++ + K D+
Sbjct 125 DKKFIFSYLQTSTIKNYFLNNLTGTTIKNLGLKTIRDTTLFVPTLEEQQKIGSFFKQLDD 184
Query 409 KFNRAISKGKKLVHLLNEYR 428
I+ ++ + LL E +
Sbjct 185 ----TIALHQRKLDLLKEQK 200
>D7EZK5 FclI S subunit [Flavobacterium columnare]
Length=393
Score = 48.1 bits (113), Expect = 4e-09
Identities = 87/437 (20%), Positives = 194/437 (44%), Gaps = 61/437 (14%)
Query 10 YKNSGVQWLGEIPSHWEVKRMKFLLSEKLKYGANESAESEDKDQPRYIRITDINDSGTLR 69
+KN+ + G IP WEVK++ +++ + A E + R +R+ + S
Sbjct 6 FKNTDI---GLIPEDWEVKQLGEVIT-LINGRAYSQNELLFNGKYRVLRVGNFFSS---- 57
Query 70 EDTFKSLEIEKAQEYLLNDLDILLARSGATVGKSYLHKKDKVNVACYAGYLIRARFNKEN 129
D + +E A ++ +N D++ A S + K + ++K + + +
Sbjct 58 -DKWYWSNLELASKFYVNKGDLMYAWSASFGPKFWKNEKTIYHYHIW-------KIELSE 109
Query 130 YDPQFINLFLQSKAYWSWIESVNIQATIQNVSAEKYNDLALSIPSLAEQKIIADFLDKRL 189
Y +F ++ K + I + + T+ +++ E + IPSL EQ+ IA+ L
Sbjct 110 YLDKFYLFYVLEKDKEN-ILNQSQGGTMFHITKESMEKRKIPIPSLKEQQAIAEVLSDTD 168
Query 190 AQVDALIAKQETLLEKLAEQRVALISHAVTKGLNPDVEMKESDVVLLGNIPNTWN-IKRL 248
A +++ LEKL ++ + A+ + L P ++ +V LG I + +
Sbjct 169 AWIES--------LEKLITKKRLVKQGAMQQLLTP---KEDWEVKKLGEIAEVRDGTHQT 217
Query 249 KFLLSEKLKYGANESAESEDKENPRYIRITDIDDSGNLKDETFKSLESEKAQEYLLDDLD 308
+ + + + ES D +N +YI ++ K L + + ++ D
Sbjct 218 PTYVESGIPFYSVESVTKNDFKNTKYI-----------SEQEHKIL----TKSFRIEKGD 262
Query 309 ILLARSGATVGKSYLYKAESVGIACYAGY-LIRARLDQENYNPEFVNYFLQSKQYWDWIS 367
IL+ R G ++G L + V + Y L++ + ++ ++ ++ +++ + I
Sbjct 263 ILMTRIG-SIGDCKLIDWD-VNASFYVSLALLKVK---PIFSANYLCHYSKTENFKKEID 317
Query 368 SINIQATI-QNVSAEKYNDLTLAIPSLEEQKQLIEYLKNED---EKFNRAISKGKKLVHL 423
++Q+ I + ++ +++ + PSL+EQ+++ L + D E + ++K K+L
Sbjct 318 INSLQSAIPKKINLGPISNVKIEFPSLDEQQRIATILSDMDAEIEHLEKKLNKAKQL--- 374
Query 424 LNEYRSTLITQVVTGKI 440
+ ++ Q++TGKI
Sbjct 375 ----KQGIMQQLLTGKI 387
>O52274 Specificity subunit [Lactococcus lactis subsp. lactis
bv. diacetylactis]
Length=409
Score = 42.4 bits (98), Expect = 3e-07
Identities = 63/273 (23%), Positives = 122/273 (45%), Gaps = 31/273 (11%)
Query 172 IPSLAEQKIIADFLDKRLAQVDALIAKQETLLEKLAEQRVALISHAVTKGLNPDVEMKES 231
I +L EQ+ I F Q+D I + L+ L EQ+ + K E++ +
Sbjct 157 IKNLEEQQKIGSFFK----QLDNTITLHQRKLDLLKEQKKGYLQKMFPKNGAKVPELRFA 212
Query 232 DVVLLGNIPNTWNIKRLKFLLSEKLKYGANESAESEDKENPRYIRITDIDDSGNLKDETF 291
V + W ++L +L + A S S D + +I T GN+
Sbjct 213 GFV------DDWEQRKLGEML---VNLEAGVSVNSSDYDTGYFILKTSAIKMGNIDLLEV 263
Query 292 KSLESE---KAQEYLLDDLDILLARSGAT--VGKSYLYKAESVGIACYAGYLIRARLDQE 346
KS+ SE +A+ L+ + I+++R VG S L + ES+ L + ++
Sbjct 264 KSIVSEEVARAKTPLIKN-SIIISRMNTPELVGASGLVR-ESIDNIFLPDRLWQGQV-AG 320
Query 347 NYNPEF----VNYFLQSKQYWDWISSINIQATIQNVSAEKYNDLTLAIPSLEEQKQLIEY 402
N++PE+ +N K+ D + + +++N+S + DL + +P+LEEQ+++ +
Sbjct 321 NFSPEWLIQSINIAANIKKIRDLATGTS--GSMKNISKKSMLDLIINVPTLEEQQKIGSF 378
Query 403 LKNEDEKFNRAISKGKKLVHLLNEYRSTLITQV 435
K D+ I+ ++ + LL E + + ++
Sbjct 379 FKQLDD----VIALHQRKLDLLKEQKKGFLQKM 407
Score = 36.6 bits (83), Expect = 2e-05
Identities = 50/207 (24%), Positives = 89/207 (43%), Gaps = 45/207 (22%)
Query 25 WEVKRMKFLLSEKLKYGANESAESEDKDQPRYIRITDINDSGTLREDTFKSL---EIEKA 81
WE +++ +L + A S S D D +I T G + KS+ E+ +A
Sbjct 218 WEQRKLGEML---VNLEAGVSVNSSDYDTGYFILKTSAIKMGNIDLLEVKSIVSEEVARA 274
Query 82 QEYLL-NDLDILLARSGATVGKSYLHKKDKVNVACYAGYLIRARFNKE---NYDPQFINL 137
+ L+ N + I + VG S L ++ N+ +L + + N+ P+++
Sbjct 275 KTPLIKNSIIISRMNTPELVGASGLVRESIDNI-----FLPDRLWQGQVAGNFSPEWL-- 327
Query 138 FLQSKAYWSWIESVNIQATIQ--------------NVSAEKYNDLALSIPSLAEQKIIAD 183
I+S+NI A I+ N+S + DL +++P+L EQ+ I
Sbjct 328 ----------IQSINIAANIKKIRDLATGTSGSMKNISKKSMLDLIINVPTLEEQQKIGS 377
Query 184 FLDKRLAQVDALIAKQETLLEKLAEQR 210
F Q+D +IA + L+ L EQ+
Sbjct 378 F----FKQLDDVIALHQRKLDLLKEQK 400
Lambda K H a alpha
0.315 0.132 0.372 0.792 4.96
Gapped
Lambda K H a alpha sigma
0.267 0.0410 0.140 1.90 42.6 43.6
Effective search space used: 2676574
Database: d0e3f0408600160beb9c8e9d771c8ef9.TrEMBL.fasta
Posted date: May 11, 2024 9:18 AM
Number of letters in database: 7,678
Number of sequences in database: 18
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 11
Window for multiple hits: 40
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Data ...
