BLASTP 2.12.0+
Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics: Alejandro A. Schaffer,
L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri
I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids
Res. 29:2994-3005.
Database: f999eb65aea7f2c27ceb8309f1f680f8.SwissProt.fasta
6 sequences; 7,387 total letters
Query= ACIAD3316
Length=1451
Score E
Sequences producing significant alignments: (Bits) Value
Q02GC2 Type IV pilus biogenesis factor PilY1 [Pseudomonas aerugin... 76.3 7e-17
Q9HVM8 Type IV pilus biogenesis factor PilY1 [Pseudomonas aerugin... 72.0 1e-15
S0HPF7 Type IV pilus biogenesis factor PilY1 [Pseudomonas aerugin... 70.1 5e-15
Q87B49 Type IV pilus biogenesis factor PilY1 homolog PD_1611 [Xyl... 58.9 1e-11
Q87FA5 Type IV pilus biogenesis factor PilY1 homolog PD_0023 [Xyl... 41.6 3e-06
Q87E25 Type IV pilus biogenesis factor PilY1 homolog PD_0502 [Xyl... 41.6 3e-06
>Q02GC2 Type IV pilus biogenesis factor PilY1 [Pseudomonas aeruginosa
(strain UCBPP-PA14)]
Length=1158
Score = 76.3 bits (186), Expect = 7e-17
Identities = 120/538 (22%), Positives = 205/538 (38%), Gaps = 136/538 (25%)
Query 695 DVKNLARWGVLGKGGWYNSASSENIANSVYSFYSDLGVTKRETFLGAPVIPT---DPLTP 751
+V +L + +G ++++ S +++ + + +++R T P + + D T
Sbjct 448 NVYDLWHAAINSRGDFFSAESPDSLVQAFNKILTR--ISERNTSSSKPAMTSALQDDGTG 505
Query 752 YNLNNYVFQNMFVPDDKQTWFGNVKKYMTDTGETIQTKTFQDVWSNQNIDQTNILSGGVI 811
L Y +Q+ F D + W G++ +Y ++ T TKT + WS +G ++
Sbjct 506 DKLIRYSYQSSFASD--KNWAGDLIRYKVESTSTGSTKTQE--WS----------AGALL 551
Query 812 DKLPIASGNNASTRQLYINRTCDT-KAKKYDVSNAISAVGNNYYTQLCAGQTTDPRRNDL 870
D A TR +YI T + K + SN + QL +P +++
Sbjct 552 DNRAPA------TRNIYIASNSGTNRLKPFTWSNIEGS-------QLATWLNRNPDKDNQ 598
Query 871 MNLLGYQ-IQSVNNQETLVA-KPEYRKIGMVLHSTPIKI--TQSATVKDDGSLTRDDYLV 926
+ G Q + + Q+ + + +G ++HS+P + Q T + DY
Sbjct 599 ADTKGAQRVDFIRGQQNMDGFRQRQAVLGDIVHSSPAVVGPAQYLTYLANPIEPSGDYGT 658
Query 927 F-------------GSAEGLLHVVNADTGVEKLAFLPNEMLENSKQRKAFTGNGLEGHDS 973
F GS +G+LH N TGVE+ AF+P + E + TG +G
Sbjct 659 FKTEADQRSPRVYVGSNDGMLHGFNIKTGVEEFAFIPTAVFEKLNK---LTGISYQG--- 712
Query 974 FSNMQYGVDGPWTAYTEYVWNSKDNKLTVGKSTTDAVCIKDGVFTGACGKQYLYGGLRMG 1033
QY VD + D F GA L G L G
Sbjct 713 -GAHQYFVDA-------------------------TPVVSDAFFDGA-WHTVLIGTLGAG 745
Query 1034 GRSYYALDLNDLAAPKLKFYIDPASGRVYSDAYPGGKSFDAIKNMGQSWSKPTIAWINWQ 1093
GR +ALD+ KL + D ++ ++G ++SKPT+A ++
Sbjct 746 GRGLFALDVTKPDDVKLLWEYDSSTD----------------SDLGYTFSKPTVARLH-- 787
Query 1094 GKRKLVMFVGGGYDAGGTDGNSNNGGYELANYNQTNKRGAGVYMFDAENGDLLWWANNLA 1153
+ + G GY G+D + A + + D + G L+ L
Sbjct 788 -SGQWAVVTGNGY---GSDNDK-----------------AALLLIDLKKGTLI---KKLE 823
Query 1154 TTSNDVSNSALNTNMQFSVVGRINTVDRDGDGLIDHLYFGDLGGQLWRVDI--NNRVD 1209
S + L+T D + DG+ D+ Y GDL G +WR D+ N R D
Sbjct 824 VQSERGIANGLSTP---------RLADNNSDGIADYAYAGDLQGNIWRFDLIGNTRND 872
Score = 20.0 bits (40), Expect = 9.4
Identities = 11/21 (52%), Positives = 14/21 (67%), Gaps = 1/21 (5%)
Query 252 RAGR-ILAPACKLSTTKASDT 271
RAG+ +LA C LS ASD+
Sbjct 7 RAGKSLLAAGCTLSILFASDS 27
>Q9HVM8 Type IV pilus biogenesis factor PilY1 [Pseudomonas aeruginosa
(strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847
/ LMG 12228 / 1C / PRS 101 / PAO1)]
Length=1161
Score = 72.0 bits (175), Expect = 1e-15
Identities = 117/525 (22%), Positives = 207/525 (39%), Gaps = 114/525 (22%)
Query 692 DAEDVKNLARWGVLGKGGWYNSASSENIANSVYSFYSDLG-VTKRETFLGAPVIPT---D 747
D+ +V +L V +G ++++ S + + V +F L ++ ++ P I + +
Sbjct 451 DSNNVYDLWHAAVNSRGEFFSADSPDQL---VAAFQDILNRISGKDLPASRPAISSSLQE 507
Query 748 PLTPYNLNNYVFQNMFVPDDKQTWFGNVKKYMTDTGE--TIQTKTFQDVWSNQNIDQTNI 805
T L + +Q F D + W G++ +Y T + T+QTK +WS Q+I +
Sbjct 508 DDTGDKLTRFAYQTSFASD--KNWAGDLTRYSLTTQDKATVQTK----LWSAQSILDA-M 560
Query 806 LSGGVIDKLPIASGNNASTRQLYINRTCDTKAKKYDVSNAISAVGNNYYTQLCAGQTTDP 865
+GG K+ +A + ++ + ++ + + V + A D
Sbjct 561 PNGGAGRKIMMAGSGTSGLKEFTWGSLSADQQRQLNRDPDRNDVADTKGQDRVAFLRGDR 620
Query 866 RRNDLMN------LLGYQIQSVNNQETLVAKPEYRKIGMVLHSTPIKITQSATVKDDGSL 919
R+ + N +LG +N+ V K +Y + + PI+ + + + +
Sbjct 621 RKENSDNFRTRNSILG---DIINSSPATVGKAQY----LTYLAQPIEPSGNYSTFAEAQK 673
Query 920 TRDDYLVFGSAEGLLHVVNADTGVEKLAFLPNEMLENSKQRKAFTGNGLEGHDSFSNMQY 979
TR + G+ +G+LH + D G E AF+P+ + E + T G +G Q+
Sbjct 674 TRAPRVYVGANDGMLHGFDTD-GNETFAFIPSAVFEKLHK---LTARGYQG----GAHQF 725
Query 980 GVDGPWTAYTEYVWNSKDNKLTVGKSTTDAVCIKDGVFTGACGKQYLYGGLRMGGRSYYA 1039
VDG + + D F GA L G LR GG+ +A
Sbjct 726 YVDG-------------------------SPVVADAFFGGA-WHTVLIGSLRAGGKGLFA 759
Query 1040 LDLNDLAAPKLKFYIDPASGRVYSDAYPGGKSFDAIKNMGQSWSKPTIAWINWQGKRKLV 1099
LD+ D A KL + I D ++G S+ KPT+A ++ GK +V
Sbjct 760 LDVTDPANIKLLWEI----------------GVDQEPDLGYSFPKPTVARLH-NGKWAVV 802
Query 1100 MFVGGGYDAGGTDGNSNNGGYELANYNQTNKRGAGVYMFDAENGDLLWWANNLATTSNDV 1159
+ NG Y+ N + A + + D E G + L T
Sbjct 803 ---------------TGNG------YSSLNDK-AALLIIDLETGAI---TRKLEVTGRTG 837
Query 1160 SNSALNTNMQFSVVGRINTVDRDGDGLIDHLYFGDLGGQLWRVDI 1204
+ L++ D + DG+ D+ Y GDL G LWR D+
Sbjct 838 VPNGLSSP---------RLADNNSDGVADYAYAGDLQGNLWRFDL 873
Score = 21.6 bits (44), Expect = 3.2
Identities = 16/59 (27%), Positives = 24/59 (41%), Gaps = 4/59 (7%)
Query 93 SGGGLGILGDKDALELVTDTVGAVDQVV----LQGLLGGSLDPLLKITTESPSSKYNYA 147
+G G L DK AL ++ GA+ + + G+ G P L + Y YA
Sbjct 803 TGNGYSSLNDKAALLIIDLETGAITRKLEVTGRTGVPNGLSSPRLADNNSDGVADYAYA 861
Score = 20.0 bits (40), Expect = 9.4
Identities = 14/48 (29%), Positives = 19/48 (40%), Gaps = 2/48 (4%)
Query 982 DGPWTAYTEYVWN--SKDNKLTVGKSTTDAVCIKDGVFTGACGKQYLY 1027
DG W + V N S L GKS + +DG F + + Y
Sbjct 318 DGLWNNDSANVGNADSTARNLPDGKSYSSQTPYRDGTFDTLADQAFHY 365
>S0HPF7 Type IV pilus biogenesis factor PilY1 [Pseudomonas aeruginosa
(strain PAK)]
Length=1163
Score = 70.1 bits (170), Expect = 5e-15
Identities = 105/464 (23%), Positives = 176/464 (38%), Gaps = 117/464 (25%)
Query 754 LNNYVFQNMFVPDDKQTWFGNVKKYMTDTGE--TIQTKTFQDVWSNQNIDQTNILSGGVI 811
L + +Q F D + W G++ +Y T + T+QTK +WS Q+I + +GG
Sbjct 516 LTRFAYQTSFASD--KNWAGDLTRYSLTTQDKATVQTK----LWSAQSILDA-MPNGGAG 568
Query 812 DKLPIAS-----------GNNASTRQLYINRTCDTKAKKYDVSNAISAVGNNYYTQLCAG 860
K+ +A G+ ++ +Q +NR D N ++ G
Sbjct 569 RKIMMAGSGTSGLKEFTWGSLSADQQRQLNRDPDR--------NDVADTKGQDRVAFLRG 620
Query 861 QTTDPRRNDLMNLLGYQIQSVNNQETLVAKPEYRKIGMVLHSTPIKITQSATVKDDGSLT 920
+ ++ +N+ V K +Y + + PI+ + + + + T
Sbjct 621 DRSKENSDNFRTRNSILGDIINSSPATVGKAQY----LTYLAQPIEPSGNYSTFAEAQKT 676
Query 921 RDDYLVFGSAEGLLHVVNADTGVEKLAFLPNEMLENSKQRKAFTGNGLEGHDSFSNMQYG 980
R + G+ +G+LH + D G E AF+P+ + E + T G +G Q+
Sbjct 677 RAPRVYVGANDGMLHGFDTD-GNETFAFIPSAVFEKLHK---LTARGYQG----GAHQFY 728
Query 981 VDGPWTAYTEYVWNSKDNKLTVGKSTTDAVCIKDGVFTGACGKQYLYGGLRMGGRSYYAL 1040
VDG + + D F GA L G LR GG+ +AL
Sbjct 729 VDG-------------------------SPVVADAFFGGA-WHTVLIGSLRAGGKGLFAL 762
Query 1041 DLNDLAAPKLKFYIDPASGRVYSDAYPGGKSFDAIKNMGQSWSKPTIAWINWQGKRKLVM 1100
D+ D A KL + I D ++G S+ KPT+A ++ GK +V
Sbjct 763 DVTDPANIKLLWEI----------------GVDQEPDLGYSFPKPTVARLH-NGKWAVV- 804
Query 1101 FVGGGYDAGGTDGNSNNGGYELANYNQTNKRGAGVYMFDAENGDLLWWANNLATTSNDVS 1160
+ NG Y+ N + A + + D E G + L T
Sbjct 805 --------------TGNG------YSSLNDK-AALLIIDLETGAI---TRKLEVTGRTGV 840
Query 1161 NSALNTNMQFSVVGRINTVDRDGDGLIDHLYFGDLGGQLWRVDI 1204
+ L++ D + DG+ D+ Y GDL G LWR D+
Sbjct 841 PNGLSSP---------RLADNNSDGVADYAYAGDLQGNLWRFDL 875
Score = 21.6 bits (44), Expect = 3.2
Identities = 16/59 (27%), Positives = 24/59 (41%), Gaps = 4/59 (7%)
Query 93 SGGGLGILGDKDALELVTDTVGAVDQVV----LQGLLGGSLDPLLKITTESPSSKYNYA 147
+G G L DK AL ++ GA+ + + G+ G P L + Y YA
Sbjct 805 TGNGYSSLNDKAALLIIDLETGAITRKLEVTGRTGVPNGLSSPRLADNNSDGVADYAYA 863
>Q87B49 Type IV pilus biogenesis factor PilY1 homolog PD_1611
[Xylella fastidiosa (strain Temecula1 / ATCC 700964)]
Length=1472
Score = 58.9 bits (141), Expect = 1e-11
Identities = 73/298 (24%), Positives = 118/298 (40%), Gaps = 76/298 (26%)
Query 910 SATVKDDGSLTRDDYLVFGSAEGLLHVVNADTGVEKLAFLPNEMLENSKQRKAFTGNGLE 969
SA + + T +++ G+ +G+LH + D G E+ A++PN +L + F N
Sbjct 976 SAFINARQNSTAAEHIFVGANDGMLHAFD-DKGTERFAYVPNGVLHHL----GFLANPEY 1030
Query 970 GHDSFSNMQYGVDGPWTAYTEYVWNSKDNKLTVGKSTTDAVCIKDGVFTGACGKQYLYGG 1029
H Y VDG T Y+ DG + + L GG
Sbjct 1031 QH------HYYVDGKSTLSDAYL---------------------DGSW-----RSVLVGG 1058
Query 1030 LRMGGRSYYALDLNDLAAPKLKFYIDPASGRVYSDAYPGGKSFDAIKNMGQSWSKPTIAW 1089
GGRS +ALD+ P++ + + D +MG + KP I
Sbjct 1059 TGAGGRSMFALDVT-----------SPSTFNESNVLWEMNSEND--DDMGYTMGKPYI-- 1103
Query 1090 INWQGKRKLVMFVGGGYDAGGTDGNSNNGGYELANYNQTNKRGAGVYMFDAENGDLLWWA 1149
V G + A +G YN TN R A +++ + G L+
Sbjct 1104 ---------VPLQNGSWAAIFGNG-----------YNSTNGR-AVLFIVNLATGQLIRKI 1142
Query 1150 NNLATTSNDVSNSALNTNMQFSVVGRINTVDRDGDGLIDHLYFGDLGGQLWRVDINNR 1207
D S+ A NM ++ +G + +D DGDGL+D +Y DL G LW+ +++ +
Sbjct 1143 EARDGIDPDGSDPA---NMGYNGLGNLAVLDTDGDGLVDMVYGADLHGNLWKFNLSGK 1197
Score = 20.8 bits (42), Expect = 5.5
Identities = 29/113 (26%), Positives = 40/113 (35%), Gaps = 7/113 (6%)
Query 1013 KDGVFTGACGKQYLYGGLRMGGRSYYALDLNDLAAPKLKFYIDPASGRVYSDAYPGGKSF 1072
K G + A +Y +MGG + LA+P KF + RV P KS
Sbjct 494 KGGTYYSAAVARY-GANHKMGGSKFVRTYAVALASPLPKFEFPVGNARV--SLVPFAKSV 550
Query 1073 DAIKNMGQSWSKPTIAWINWQGKRKLVMFVGGGYDAGGTDGNSNNGGYELANY 1125
+PT I++ +R M G D T NGG A +
Sbjct 551 RGFGISATGNFQPTNQIIDFYVQRVANMAGSSGADYDAT----INGGRPYAEF 599
>Q87FA5 Type IV pilus biogenesis factor PilY1 homolog PD_0023
[Xylella fastidiosa (strain Temecula1 / ATCC 700964)]
Length=1214
Score = 41.6 bits (96), Expect = 3e-06
Identities = 68/274 (25%), Positives = 98/274 (36%), Gaps = 89/274 (32%)
Query 928 GSAEGLLHVVNADTGVEKLAFLPNEMLENSKQRKAFTGNGLEGHDSFSNMQYGVDGPWTA 987
G+ +G+LH +A+TG E AF+P +A LE D QY VDG TA
Sbjct 787 GANDGMLHAFDANTGKEIFAFVP----------RAAMPKLLEYTDQNYGHQYYVDGELTA 836
Query 988 YTEYVWNSKDNKLTVGKSTTDAVCIKDGVFTGACGKQYLYGGLRMGGRSYYALDLNDLAA 1047
Y D KL + L G L GG+ +ALD+
Sbjct 837 ADIY-----DTKLG--------------------WRSVLVGTLGRGGKGLFALDVT---- 867
Query 1048 PKLKFYIDPASGRVYSDAYPGGKSFDAIKNMGQSWSKPTIAWINWQGKRKLVMFVGGGYD 1107
DP++ R+ D K+ I +G + SKP IA + G +++
Sbjct 868 -------DPSNIRLLWD-----KTSADIGGLGNTLSKPMIAQTS-DGTWSVLL------- 907
Query 1108 AGGTDGNSNNGGYELANYNQTNKRGAGVYMFDAENGDLLWWANNLATTSNDVSNSALNTN 1167
G NS +L N A V + + SN+ L+
Sbjct 908 --GNGPNSTADNAQLIVMNLLTGHAAQVPV-------------------SKTSNNGLSGV 946
Query 1168 MQFSVVGRINTVDRDGDGLIDHLYFGDLGGQLWR 1201
+S +G+ D +Y GDL G LWR
Sbjct 947 FPWS---------SQSNGITDRVYAGDLLGTLWR 971
Score = 23.5 bits (49), Expect = 0.83
Identities = 31/125 (25%), Positives = 50/125 (40%), Gaps = 14/125 (11%)
Query 613 YSKKLLDKNVSTGVIGVGREFITIPSTKDSAQLE-------ASMSNLNNSLLGQTVPNLV 665
Y KL ++V G +G G + + D + + A + L N+L +
Sbjct 840 YDTKLGWRSVLVGTLGRGGKGLFALDVTDPSNIRLLWDKTSADIGGLGNTLSKPMIAQTS 899
Query 666 KDTLNVLLG-GP--LVNGLTNLLGNLFPTDAED--VKNLARWGVLGKGGWYNSASSENIA 720
T +VLLG GP + ++ NL A V + G+ G W S+ S I
Sbjct 900 DGTWSVLLGNGPNSTADNAQLIVMNLLTGHAAQVPVSKTSNNGLSGVFPW--SSQSNGIT 957
Query 721 NSVYS 725
+ VY+
Sbjct 958 DRVYA 962
Score = 22.3 bits (46), Expect = 1.9
Identities = 18/60 (30%), Positives = 29/60 (48%), Gaps = 10/60 (17%)
Query 567 GRSLDTQATDLSRLDLQSVFQ-------CDESAGLKSTTLS--SRDYATWSCIGNYSKKL 617
G+ D+ L+R D + V++ CD SAGL+S ++ Y I YSK++
Sbjct 218 GQPYDSSRHPLNRND-RGVYEVSVRVKVCDPSAGLESNCVAYPRGSYKPEGLIQEYSKRI 276
>Q87E25 Type IV pilus biogenesis factor PilY1 homolog PD_0502
[Xylella fastidiosa (strain Temecula1 / ATCC 700964)]
Length=1219
Score = 41.6 bits (96), Expect = 3e-06
Identities = 68/274 (25%), Positives = 98/274 (36%), Gaps = 89/274 (32%)
Query 928 GSAEGLLHVVNADTGVEKLAFLPNEMLENSKQRKAFTGNGLEGHDSFSNMQYGVDGPWTA 987
G+ +G+LH +A+TG E AF+P +A LE D QY VDG TA
Sbjct 793 GANDGMLHAFDANTGKEIFAFVP----------RAAMPKLLEYTDQNYGHQYYVDGELTA 842
Query 988 YTEYVWNSKDNKLTVGKSTTDAVCIKDGVFTGACGKQYLYGGLRMGGRSYYALDLNDLAA 1047
Y D KL + L G L GG+ +ALD+
Sbjct 843 ADIY-----DTKLG--------------------WRSVLVGTLGRGGKGLFALDVT---- 873
Query 1048 PKLKFYIDPASGRVYSDAYPGGKSFDAIKNMGQSWSKPTIAWINWQGKRKLVMFVGGGYD 1107
DP++ R+ D K+ I +G + SKP IA + G +++
Sbjct 874 -------DPSNIRLLWD-----KTSADIGGLGNTLSKPMIAQTS-DGTWSVLL------- 913
Query 1108 AGGTDGNSNNGGYELANYNQTNKRGAGVYMFDAENGDLLWWANNLATTSNDVSNSALNTN 1167
G NS +L N A V + + SN+ L+
Sbjct 914 --GNGPNSTADNAQLIVMNLLTGHAAQVPV-------------------SKTSNNGLSGV 952
Query 1168 MQFSVVGRINTVDRDGDGLIDHLYFGDLGGQLWR 1201
+S +G+ D +Y GDL G LWR
Sbjct 953 FPWS---------SQSNGITDRVYAGDLLGTLWR 977
Score = 23.5 bits (49), Expect = 0.83
Identities = 31/125 (25%), Positives = 50/125 (40%), Gaps = 14/125 (11%)
Query 613 YSKKLLDKNVSTGVIGVGREFITIPSTKDSAQLE-------ASMSNLNNSLLGQTVPNLV 665
Y KL ++V G +G G + + D + + A + L N+L +
Sbjct 846 YDTKLGWRSVLVGTLGRGGKGLFALDVTDPSNIRLLWDKTSADIGGLGNTLSKPMIAQTS 905
Query 666 KDTLNVLLG-GP--LVNGLTNLLGNLFPTDAED--VKNLARWGVLGKGGWYNSASSENIA 720
T +VLLG GP + ++ NL A V + G+ G W S+ S I
Sbjct 906 DGTWSVLLGNGPNSTADNAQLIVMNLLTGHAAQVPVSKTSNNGLSGVFPW--SSQSNGIT 963
Query 721 NSVYS 725
+ VY+
Sbjct 964 DRVYA 968
Score = 22.7 bits (47), Expect = 1.4
Identities = 24/82 (29%), Positives = 33/82 (40%), Gaps = 18/82 (22%)
Query 554 EGLGTKNAIERLMGRSLDT-----QATDLSRLDLQS-----------VFQCDESAGLKST 597
+GLG + + G S D Q D SR L S V CD S GL+S
Sbjct 199 DGLGNRMRFTQFPGLSTDPLNTEGQPYDPSRHPLNSNDRGVYEVSVRVKVCDASVGLESN 258
Query 598 TLS--SRDYATWSCIGNYSKKL 617
++ S Y + YSK++
Sbjct 259 CVAYPSGFYKPEGLVQEYSKRV 280
Lambda K H a alpha
0.316 0.134 0.394 0.792 4.96
Gapped
Lambda K H a alpha sigma
0.267 0.0410 0.140 1.90 42.6 43.6
Effective search space used: 9759190
Database: f999eb65aea7f2c27ceb8309f1f680f8.SwissProt.fasta
Posted date: May 20, 2024 11:36 PM
Number of letters in database: 7,387
Number of sequences in database: 6
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 11
Window for multiple hits: 40
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Data ...
