BLASTP 2.12.0+
Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics: Alejandro A. Schaffer,
L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri
I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids
Res. 29:2994-3005.
Database: 3efd95f8ca2f68fc410532da04f5f59b.SwissProt.fasta
13 sequences; 2,772 total letters
Query= ACIAD0017
Length=205
Score E
Sequences producing significant alignments: (Bits) Value
Q52828 Protein GstA [Rhizobium leguminosarum] 107 1e-32
Q93112 Glutathione S-transferase 1, isoform C [Anopheles gambiae] 81.3 1e-22
P57108 Glutathione S-transferase zeta class [Euphorbia esula] 80.1 4e-22
P77526 Disulfide-bond oxidoreductase YfcG [Escherichia coli (stra... 79.3 6e-22
Q9VG97 Inactive glutathione S-transferase D3 [Drosophila melanoga... 76.6 4e-21
Q93113 Glutathione S-transferase 1, isoform D [Anopheles gambiae] 75.9 1e-20
B3FWR8 Glutathione S-transferase hmp2 [Hypomyces subiculosus] 72.8 2e-19
O77473 Glutathione S-transferase 1, isoform B [Anopheles gambiae] 71.6 4e-19
Q9LZI9 Glutathione S-transferase F13 [Arabidopsis thaliana] 69.7 2e-18
Q94999 Glutathione S-transferase 2 [Anopheles gambiae] 68.6 5e-18
Q9VG95 Glutathione S-transferase D5 [Drosophila melanogaster] 68.2 8e-18
A0A6J4B5J2 Glutathione S-transferase-like protein LUC7 [Fusarium sp] 67.8 1e-17
P28342 Glutathione S-transferase 1 [Dianthus caryophyllus] 67.4 2e-17
>Q52828 Protein GstA [Rhizobium leguminosarum]
Length=203
Score = 107 bits (266), Expect = 1e-32
Identities = 62/201 (31%), Positives = 113/201 (56%), Gaps = 13/201 (6%)
Query 1 MKVYGDIQSGNCYKVKLLLNFLELAHEWIHIDVLKAETHTPEFLKMNPNAKIPVVQLEDG 60
MK+Y SG+ ++ L L+ L + +E + +D+ P+FLK+NP ++PV+ ++G
Sbjct 1 MKLYHHPLSGHAHRAHLFLSLLGVPYELVEVDLAAGAHKAPDFLKLNPFGQVPVLD-DNG 59
Query 61 QLLAESNAILGYLAEG---THFIPSDRYKKAKMYEWMFFE--QYSHEPCIAVARFIQKYQ 115
++A+S+AIL YLA T ++P + A++ +W+ + ++ PC AR + +
Sbjct 60 TVIADSSAILVYLARKYGRTDWLPEEAVAAARIQKWLSVAAGEIAYGPC--AARLVTVFG 117
Query 116 GMPEARLEEYQKLQPKGHKVLAILEQALDGHHYLLGEEVSLADISLYAYTHVADEGGFDL 175
+ R +E + + H++LA++E L +LLG+ ++ADI+LY+Y A EG D
Sbjct 118 A--DFRTDE---VIARAHRILALVEAELGARRFLLGDNATIADIALYSYIANAPEGNVDT 172
Query 176 NLYPNIQRWCQRIANSAGYVG 196
+ Y ++ W RI G+VG
Sbjct 173 SAYQAVRAWLARIEALPGFVG 193
>Q93112 Glutathione S-transferase 1, isoform C [Anopheles gambiae]
Length=209
Score = 81.3 bits (199), Expect = 1e-22
Identities = 56/171 (33%), Positives = 85/171 (50%), Gaps = 10/171 (6%)
Query 32 DVLKAETHTPEFLKMNPNAKIPVVQLEDGQLLAESNAILGYLAE----GTHFIPSDRYKK 87
D++K E PEFLK+NP IP + +++G L ES AI YLAE P D K+
Sbjct 32 DLMKGEHMKPEFLKINPQHCIPTL-VDNGFALWESRAICTYLAEKYGKDDKLYPKDPQKR 90
Query 88 AKMYEWMFFEQYSHEPCIAVARFIQKYQGMPEARLEEYQKLQPKGHKVLAILEQALDGHH 147
A + + M+F+ + A + Q + P A E QK++ + L LDGH
Sbjct 91 AVVNQRMYFDMGTLYQRFADYYYPQIFAKQP-ANPENEQKMKD----AVGFLNSFLDGHK 145
Query 148 YLLGEEVSLADISLYAYTHVADEGGFDLNLYPNIQRWCQRIANSAGYVGMN 198
Y+ G+ +++AD+S+ A D GFDL Y ++ W + I A +N
Sbjct 146 YVAGDSLTIADLSILATISTYDVAGFDLAKYQHVAAWYENIRKEAPGAAIN 196
>P57108 Glutathione S-transferase zeta class [Euphorbia esula]
Length=225
Score = 80.1 bits (196), Expect = 4e-22
Identities = 64/192 (33%), Positives = 103/192 (54%), Gaps = 15/192 (8%)
Query 1 MKVYGDIQSGNCYKVKLLLNFLELAHEWIHIDVLKAETHTPEFLKMNPNAKIPVVQLEDG 60
+K+Y +S ++V++ LN L +E++ +++LK E TPEFLK+NP +P L DG
Sbjct 12 LKLYSYFRSSCSFRVRIALNLKGLDYEYVPVNLLKGEQFTPEFLKINPIGYVPA--LVDG 69
Query 61 Q-LLAESNAILGYLAE---GTHFIPSDRYKKAKMYEWMFFEQYSHEPC--IAVARFIQKY 114
+ ++++S AIL YL E +P+D +KKA Y+ S +P +AV FI +
Sbjct 70 EDVISDSFAILMYLEEKYPEHPILPADIHKKAINYQAANIVSSSIQPLQNLAVLNFIGE- 128
Query 115 QGMPEARLEEYQKLQPKGHKVLAILEQALDGH--HYLLGEEVSLADISLYAYTHVA-DEG 171
+ P+ ++ Q+ KG A LE+ L GH + G+EV LAD+ L H A
Sbjct 129 KVSPDEKVPWVQRHISKG---FAALEKLLQGHAGRFATGDEVYLADLFLEPQIHAAITRF 185
Query 172 GFDLNLYPNIQR 183
D+ +P + R
Sbjct 186 NVDMTQFPLLLR 197
>P77526 Disulfide-bond oxidoreductase YfcG [Escherichia coli (strain
K12)]
Length=215
Score = 79.3 bits (194), Expect = 6e-22
Identities = 53/185 (29%), Positives = 93/185 (50%), Gaps = 9/185 (5%)
Query 11 NCYKVKLLLNFLELAHEWIHIDVLKAETHTPEFLKMNPNAKIPVV----QLEDGQLLA-- 64
N +K+ L L EL + I +D+ K PEFL+++PN KIP + + G+ L+
Sbjct 11 NGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFLRISPNNKIPAIVDHSPADGGEPLSLF 70
Query 65 ESNAILGYLAEGTH-FIPSDRYKKAKMYEWMFFEQYSHEPCIAVARFIQKYQGMPEARLE 123
ES AIL YLAE T F+ + ++A +W+F++ P + P+
Sbjct 71 ESGAILLYLAEKTGLFLSHETRERAATLQWLFWQVGGLGPMLGQNHHFN--HAAPQTIPY 128
Query 124 EYQKLQPKGHKVLAILEQALDGHHYLLGEEVSLADISLYAYTHVADEGGFDLNLYPNIQR 183
++ Q + ++ +L + L+ +L GE S+ADI+ + + + DL +YP ++
Sbjct 129 AIERYQVETQRLYHVLNKRLENSPWLGGENYSIADIACWPWVNAWTRQRIDLAMYPAVKN 188
Query 184 WCQRI 188
W +RI
Sbjct 189 WHERI 193
Score = 20.8 bits (42), Expect = 0.18
Identities = 7/13 (54%), Positives = 9/13 (69%), Gaps = 0/13 (0%)
Query 130 PKGHKVLAILEQA 142
P GHK+ LE+A
Sbjct 10 PNGHKITLFLEEA 22
>Q9VG97 Inactive glutathione S-transferase D3 [Drosophila melanogaster]
Length=199
Score = 76.6 bits (187), Expect = 4e-21
Identities = 50/162 (31%), Positives = 84/162 (52%), Gaps = 10/162 (6%)
Query 31 IDVLKAETHTPEFLKMNPNAKIPVVQLEDGQLLAESNAILGYLAE----GTHFIPSDRYK 86
I+ LK E P+F+K+NP IP + +++G + ES AIL YL E P D K
Sbjct 15 INTLKGEQMNPDFIKINPQHSIPTL-VDNGFTIWESRAILVYLVEKYGKDDALYPKDIQK 73
Query 87 KAKMYEWMFFEQYSHEPCIAVARFIQKYQGMPEARLEEYQKLQPKGHKVLAILEQALDGH 146
+A + + ++F+ P +A + G + E+Y+K+Q + L L+G
Sbjct 74 QAVINQRLYFDMALMYPTLANYYYKAFTTGQFGSE-EDYKKVQ----ETFDFLNTFLEGQ 128
Query 147 HYLLGEEVSLADISLYAYTHVADEGGFDLNLYPNIQRWCQRI 188
Y+ G++ ++ADI++ A D GFD++ YPN+ RW +
Sbjct 129 DYVAGDQYTVADIAILANVSNFDVVGFDISKYPNVARWYDHV 170
>Q93113 Glutathione S-transferase 1, isoform D [Anopheles gambiae]
Length=209
Score = 75.9 bits (185), Expect = 1e-20
Identities = 55/168 (33%), Positives = 84/168 (50%), Gaps = 11/168 (7%)
Query 32 DVLKAETHTPEFLKMNPNAKIPVVQLEDGQLLAESNAILGYLAE----GTHFIPSDRYKK 87
D++K E PEFLK+NP IP + +++G L ES AI YLAE P D K+
Sbjct 32 DLMKGEHMKPEFLKLNPQHCIPTL-VDNGFALWESRAIQIYLAEKYGKDDKLYPKDPQKR 90
Query 88 AKMYEWMFFEQYSHEPCIAVARFIQKYQGMPEARLEEYQKLQPKGHKVLAILEQALDGHH 147
A + + ++F+ + A + Q + P A E +K++ + L L+G
Sbjct 91 AVVNQRLYFDMGTLYQRFADYHYPQIFAKQP-ANPENEKKMKD----AVGFLNTFLEGQE 145
Query 148 YLLGEEVSLADISLYAYTHVADEGGFDLNLYPNIQRWCQRI-ANSAGY 194
Y G ++++AD+SL A + GFD YPN+ W R AN+ GY
Sbjct 146 YAAGNDLTIADLSLAATIATYEVAGFDFAPYPNVAAWFARCKANAPGY 193
>B3FWR8 Glutathione S-transferase hmp2 [Hypomyces subiculosus]
Length=212
Score = 72.8 bits (177), Expect = 2e-19
Identities = 56/197 (28%), Positives = 91/197 (46%), Gaps = 18/197 (9%)
Query 1 MKVYGDIQSGNCYKVKLLLNFLELAHEWIHIDVLKAETHTPEFLKMNPNAKIPVVQLEDG 60
+K+YG S V LL EL +E I +D+ K + + E+LK+ P K+PV+ ++G
Sbjct 3 IKLYGSAMSTARVLVTLLEK--ELPYEHILVDISKGDQNKEEYLKLQPFGKVPVLD-DNG 59
Query 61 QLLAESNAILGYLA----EGTHFIPS-------DRYKKAKMYEWMFFEQYSHEPCIAVAR 109
++ ES AI YLA GT IP R+++ E+ +F + +
Sbjct 60 FIMYESRAICRYLARKYDSGTKLIPDVDDHEAYGRFEQGCSIEYSYFAAAAE--TLGTEL 117
Query 110 FIQKYQGMPEARLEEYQKLQPKGHKVLAILEQALDGHHYLLGEEVSLADISLYAYTHVAD 169
I+KY+G+ E + + + KV ++ L YL G+E+SL D+
Sbjct 118 VIKKYKGLGEPDMTRVAQAEADFDKVFFEYDKILAKQKYLTGDEISLVDLFHLPNASALK 177
Query 170 EGGFD--LNLYPNIQRW 184
G+ YPN+ RW
Sbjct 178 AFGYQGTFEKYPNVNRW 194
>O77473 Glutathione S-transferase 1, isoform B [Anopheles gambiae]
Length=216
Score = 71.6 bits (174), Expect = 4e-19
Identities = 51/161 (32%), Positives = 82/161 (51%), Gaps = 14/161 (9%)
Query 32 DVLKAETHTPEFLKMNPNAKIPVVQLEDGQLLAESNAILGYLAE-------GTHFIPSDR 84
D++K E PEFLK+NP +P + +++G L ES AI+ YL E P+D
Sbjct 32 DLMKGEHMKPEFLKLNPQHCVPTL-VDNGFALWESRAIMCYLVEKYGKPCNNDSLYPTDP 90
Query 85 YKKAKMYEWMFFEQYSHEPCIAVARFIQKYQGMPEARLEEYQKLQPKGHKVLAILEQALD 144
K+A + + ++F+ + + Q ++G P A + K+ + LA L+ L+
Sbjct 91 QKRAIVNQRLYFDMGTLYQRFGDYYYPQIFEGAP-ANETNFAKIG----EALAFLDTFLE 145
Query 145 GHHYLLGEE-VSLADISLYAYTHVADEGGFDLNLYPNIQRW 184
G ++ G SLADISLYA + G+D + Y N+ RW
Sbjct 146 GERFVAGGNGYSLADISLYATLTTFEVAGYDFSAYVNVLRW 186
>Q9LZI9 Glutathione S-transferase F13 [Arabidopsis thaliana]
Length=219
Score = 69.7 bits (169), Expect = 2e-18
Identities = 61/215 (28%), Positives = 99/215 (46%), Gaps = 17/215 (8%)
Query 1 MKVYGDIQSGNCYKVKLLLNFLELAHEWIHIDVLKAETHTPEFLKMNPNAKIPVVQLEDG 60
MK+YGD S +V L L+ E + +++ P FL MNP K+P +Q +D
Sbjct 3 MKLYGDEMSACVARVLLCLHEKNTEFELVPVNLFACHHKLPSFLSMNPFGKVPALQ-DDD 61
Query 61 QLLAESNAILGYLAE-----GTHFIPSDRYKKAKMYE-WMFFEQYSHEPCIAVA---RFI 111
L ES AI Y+AE GT + K+A + + W E + P I+ +
Sbjct 62 LTLFESRAITAYIAEKHRDKGTDLTRHEDPKEAAIVKLWSEVEAHHFNPAISAVIHQLIV 121
Query 112 QKYQG-MPEARLEEYQKLQPKGHKVLAILEQALDGHHYLLGEEVSLADISLYAYTHV--- 167
QG P A + E + L+ G K+L + E+ L YL G+ +LAD+ YT+
Sbjct 122 VPLQGESPNAAIVE-ENLENLG-KILDVYEERLGKTKYLAGDTYTLADLHHVPYTYYFMK 179
Query 168 ADEGGFDLNLYPNIQRWCQRIANSAGYVGMNTQTT 202
G +N PN++ W + + + ++ ++ T
Sbjct 180 TIHAGL-INDRPNVKAWWEDLCSRPAFLKVSPGLT 213
>Q94999 Glutathione S-transferase 2 [Anopheles gambiae]
Length=209
Score = 68.6 bits (166), Expect = 5e-18
Identities = 54/188 (29%), Positives = 87/188 (46%), Gaps = 25/188 (13%)
Query 9 SGNCYKVKLLLNFLELAHEWIHIDVLKAETHTPEFLKMNPNAKIPVVQLEDGQL-LAESN 67
S C V+++ + + ++D++ +P+F K+NP IP L DG L L+ES
Sbjct 10 SAPCRAVQMVAEAVHVKLNLKYLDLMAGAHRSPQFTKLNPQRTIPT--LVDGSLILSESR 67
Query 68 AILGYLA-----EGTHFIPSDRYKKAKMYEWMFFEQYSHEPCIAVARFIQKYQGMPEARL 122
A L YL E + P D ++A + + +FF+ C+ RF Y
Sbjct 68 AALIYLCDQYGDEDNDWYPRDTIQRAIVNQRLFFDA-----CVLYPRFADFYHP------ 116
Query 123 EEYQKLQPKGHKVLA------ILEQALDGHHYLLGEEVSLADISLYAYTHVADEGGFDLN 176
+ + P G K LA +L L H ++ G ++++ADISL+A A GF L
Sbjct 117 QVFGNAAPDGRKRLAFEKAVELLNIFLSEHEFVAGSKMTIADISLFATLATACTLGFILR 176
Query 177 LYPNIQRW 184
Y ++ RW
Sbjct 177 PYVHVDRW 184
>Q9VG95 Glutathione S-transferase D5 [Drosophila melanogaster]
Length=216
Score = 68.2 bits (165), Expect = 8e-18
Identities = 49/188 (26%), Positives = 91/188 (48%), Gaps = 10/188 (5%)
Query 1 MKVYGDIQSGNCYKVKLLLNFLELAHEWIHIDVLKAETHTPEFLKMNPNAKIPVVQLEDG 60
M Y + C V ++ L + ++ L+ + PEF+K+NP IP + +++G
Sbjct 1 MDFYYSPRGSGCRTVIMVAKALGVKLNMKLLNTLEKDQLKPEFVKLNPQHTIPTL-VDNG 59
Query 61 QLLAESNAILGYLAE----GTHFIPSDRYKKAKMYEWMFFEQYSHEPCIAVARFIQKYQG 116
+ ES AI YL E P D K+A + + ++F+ + A + + G
Sbjct 60 FSIWESRAIAVYLVEKYGKDDTLFPKDPKKQALVNQRLYFDMGTLYDSFAKYYYPLFHTG 119
Query 117 MPEARLEEYQKLQPKGHKVLAILEQALDGHHYLLGEEVSLADISLYAYTHVADEGGFDLN 176
P + E+++K++ L L+G +Y+ G+ +++ADI++ + + FDLN
Sbjct 120 KPGSD-EDFKKIESS----FEYLNIFLEGQNYVAGDHLTVADIAILSTVSTFEIFDFDLN 174
Query 177 LYPNIQRW 184
YPN+ RW
Sbjct 175 KYPNVARW 182
>A0A6J4B5J2 Glutathione S-transferase-like protein LUC7 [Fusarium
sp]
Length=219
Score = 67.8 bits (164), Expect = 1e-17
Identities = 60/206 (29%), Positives = 97/206 (47%), Gaps = 36/206 (17%)
Query 2 KVYGDIQSGNCYKV--KLLLNFLELA-HEWIHIDVLKAETHTPEFLKMNPNAKIPVVQLE 58
++Y + +G +K+ LN LE+ + H+ + TPEF P K+P +
Sbjct 6 RLYSFMPNGRVFKILAAATLNGLEIEITPYQHM----VDNKTPEFRAKFPAGKVPAFEGA 61
Query 59 DGQLLAESNAILGYLAE----GTHFIPSDRYKKAKMYEWMFF---EQYSHEP-----CIA 106
DG LL ES+AI YLA+ + D AK+ +W+ F E Y H +
Sbjct 62 DGLLLPESDAIAQYLAQSGPYSEQLLGRDAATSAKIRQWISFFDGEVYPHMLDLVIWRVG 121
Query 107 VARFIQKYQGMPEARLEEYQKLQPKGHKVLAILEQALDGHHYLLGEEVSLADIS-----L 161
+A F Q + ARLE L +LE+ LDG +L+G+E++LAD++ L
Sbjct 122 IAPFDQSTETKALARLE----------FALDVLEKHLDGRKWLVGDELTLADLTGASSLL 171
Query 162 YAYTHVADEGGFDLNLYPNIQRWCQR 187
+A+ H+ D + +P++ W R
Sbjct 172 WAFMHIIDAS--ERKRFPSVVAWYLR 195
>P28342 Glutathione S-transferase 1 [Dianthus caryophyllus]
Length=221
Score = 67.4 bits (163), Expect = 2e-17
Identities = 64/214 (30%), Positives = 102/214 (48%), Gaps = 34/214 (16%)
Query 1 MKVYGDIQSGNCYKVKLLLNFLELAHEWIHIDVLKAETHTPEFLKMNPNAKIPVVQLEDG 60
M++Y S ++V++ L+ L E+ +D+ K E TPEFLK+NP +PV+ +
Sbjct 9 MQLYSFSLSSCAWRVRIALHLKGLDFEYKAVDLFKGEHLTPEFLKLNPLGYVPVL-VHGD 67
Query 61 QLLAESNAILGYLAE---GTHFIPSDRYKKAKMYEWMFFEQYSHEPC--IAVARFIQKYQ 115
++A+S AI+ YL E +P D K+A Y+ + +P +AV +I++
Sbjct 68 IVIADSLAIIMYLEEKFPENPLLPQDLQKRALNYQAANIVTSNIQPLQNLAVLNYIEEKL 127
Query 116 GMPEARLEEYQKLQPKGH---KVLAILEQALDGH--HYLLGEEVSLADISLYAYTHVADE 170
G E KL H K + LE+ L GH Y G+EV LAD+ L A +A
Sbjct 128 GSDE-------KLSWAKHHIKKGFSALEKLLKGHAGKYATGDEVGLADLFL-APQIIASI 179
Query 171 GGFDLNL---------------YPNIQRWCQRIA 189
GF +++ Y + + CQRI+
Sbjct 180 TGFGMDMAEFPLLKSLNDAYLKYQHFRMRCQRIS 213
Lambda K H a alpha
0.323 0.138 0.425 0.792 4.96
Gapped
Lambda K H a alpha sigma
0.267 0.0410 0.140 1.90 42.6 43.6
Effective search space used: 380426
Database: 3efd95f8ca2f68fc410532da04f5f59b.SwissProt.fasta
Posted date: May 20, 2024 12:45 AM
Number of letters in database: 2,772
Number of sequences in database: 13
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 11
Window for multiple hits: 40
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Data ...
