BLASTP 2.12.0+
Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics: Alejandro A. Schaffer,
L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri
I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids
Res. 29:2994-3005.
Database: c70766d9fe65f69657b4b8a63a21bbdf.SwissProt.fasta
18 sequences; 5,972 total letters
Query= ACIAD0032
Length=291
Score E
Sequences producing significant alignments: (Bits) Value
Q0QLF5 2-(hydroxymethyl)glutarate dehydrogenase [Eubacterium bark... 158 5e-50
O33730 Uncharacterized oxidoreductase Sfri_1503 [Shewanella frigi... 142 4e-44
P0ABQ3 2-hydroxy-3-oxopropionate reductase [Escherichia coli O6:H... 133 2e-40
P0ABQ2 2-hydroxy-3-oxopropionate reductase [Escherichia coli (str... 133 2e-40
Q54CX6 Probable 3-hydroxyisobutyrate dehydrogenase, mitochondrial... 131 2e-39
P77161 2-hydroxy-3-oxopropionate reductase [Escherichia coli (str... 127 2e-38
O34948 Uncharacterized oxidoreductase YkwC [Bacillus subtilis (st... 125 1e-37
Q9I5I6 NAD-dependent L-serine dehydrogenase [Pseudomonas aerugino... 122 4e-36
Q9SZE1 Probable 3-hydroxyisobutyrate dehydrogenase-like 1, mitoch... 120 2e-35
P9WNY5 Probable 3-hydroxyisobutyrate dehydrogenase [Mycobacterium... 116 4e-34
P9WNY4 Probable 3-hydroxyisobutyrate dehydrogenase [Mycobacterium... 116 4e-34
P63936 Probable 3-hydroxyisobutyrate dehydrogenase [Mycobacterium... 116 4e-34
Q9V8M5 Probable 3-hydroxyisobutyrate dehydrogenase, mitochondrial... 115 3e-33
F4I907 Glyoxylate/succinic semialdehyde reductase 2, chloroplasti... 106 9e-30
P28811 3-hydroxyisobutyrate dehydrogenase [Pseudomonas aeruginosa... 105 1e-29
P0A9V8 3-sulfolactaldehyde reductase [Escherichia coli (strain K12)] 95.5 3e-26
Q49A26 Cytokine-like nuclear factor N-PAC [Homo sapiens] 94.4 6e-25
Q922P9 Cytokine-like nuclear factor N-PAC [Mus musculus] 92.8 2e-24
>Q0QLF5 2-(hydroxymethyl)glutarate dehydrogenase [Eubacterium
barkeri]
Length=301
Score = 158 bits (400), Expect = 5e-50
Identities = 103/288 (36%), Positives = 158/288 (55%), Gaps = 8/288 (3%)
Query 1 MRIGFIGLGAMGWHMAGHLTQLDATVMVWNRTQHKAEAHAMQFGSLAVELEQLVQA--DL 58
++IGFIGLGAMG MA +L + TV ++ + A Q G+ A E Q V A D+
Sbjct 5 IKIGFIGLGAMGKPMAINLLKEGVTVYAFDLMEANVAAVVAQ-GAQACENNQKVAAASDI 63
Query 59 IFSCLPTSAEVEAVI-----AAYPPRSGSVWIDCTSGVPASAQQLSRHLKQHGVDFLDAP 113
IF+ LP + VE V+ ++G+V +D +S P+S ++++ + G+D++DAP
Sbjct 64 IFTSLPNAGIVETVMNGPGGVLSACKAGTVIVDMSSVSPSSTLKMAKVAAEKGIDYVDAP 123
Query 114 VSGQTIGAEKGTLTVMIGGERTAFERAQPIIQAFAGLIEHVGDSGAGFAVKAVNNILMAA 173
VSG T GAE GTLT+M+G FE+ QP++ I HVGD+GAG AVK VNN+L+
Sbjct 124 VSGGTKGAEAGTLTIMVGASEAVFEKIQPVLSVIGKDIYHVGDTGAGDAVKIVNNLLLGC 183
Query 174 NLWALSEGMSVLKAHAVHLDAALACINHSSGKSTMSETIMPQKILSRGFHKTFALNLLQK 233
N+ +L+E + + + + I SSG+S E M + I+S F FA++L K
Sbjct 184 NMASLAEALVLGVKCGLKPETMQEIIGKSSGRSYAMEAKMEKFIMSGDFAGGFAMDLQHK 243
Query 234 DIGIAIDLIKAVGQPADVLHCVQQMYSEMDLERATQVDFSAAIQTLEQ 281
D+G+A++ K P + Q++ + D SA I+ EQ
Sbjct 244 DLGLALEAGKEGNVPLPMTAMATQIFEGGRAMGLGREDMSAVIKVWEQ 291
>O33730 Uncharacterized oxidoreductase Sfri_1503 [Shewanella frigidimarina
(strain NCIMB 400)]
Length=291
Score = 142 bits (359), Expect = 4e-44
Identities = 89/288 (31%), Positives = 153/288 (53%), Gaps = 10/288 (3%)
Query 2 RIGFIGLGAMGWHMAGHLTQLDATVMVWNRTQHKAEAHAMQFGSLAVEL--EQLVQADLI 59
++ FIGLG MG+ MAGHL + V V+NRT KA Q+G + + D++
Sbjct 3 KVAFIGLGVMGFPMAGHLVKQGHDVTVYNRTGAKATQWVEQYGGKKADTPKDAAQGQDIV 62
Query 60 FSCLPTSAEVEAVIAA-----YPPRSGSVWIDCTSGVPASAQQLSRHLKQHGVDFLDAPV 114
F+C+ ++ V+ + +G++ +D T+ A++++ +++ + FLDAPV
Sbjct 63 FTCVGNDDDLRQVVLGEHGIVHGMHAGAILVDHTTASADVAREIAAYIEPLNIAFLDAPV 122
Query 115 SGQTIGAEKGTLTVMIGGERTAFERAQPIIQAFAGLIEHVGDSGAGFAVKAVNNILMAAN 174
SG GAE G LTVM+GG++ F+ +P+I A++ E +G GAG K VN I +A
Sbjct 123 SGGQAGAENGALTVMMGGDQAHFDTVKPVISAYSRCAELLGPVGAGQLTKMVNQICIAGV 182
Query 175 LWALSEGMSVLKAHAVHLDAALACINHSSGKSTMSETIMPQKILSRG-FHKTFALNLLQK 233
+ L+EG+ K+ + + I+ + +S E K + +G + FA++ ++K
Sbjct 183 VQGLAEGLHFAKSAGLDGLKVIEVISKGAAQSWQMEN--RYKTMWQGQYDFGFAIDWMRK 240
Query 234 DIGIAIDLIKAVGQPADVLHCVQQMYSEMDLERATQVDFSAAIQTLEQ 281
D+GIA+D + G V V Q YSE+ + + D S+ + LE+
Sbjct 241 DLGIALDEARRNGSHLPVAALVDQFYSEVQAMKGNRWDTSSLLARLEK 288
>P0ABQ3 2-hydroxy-3-oxopropionate reductase [Escherichia coli
O6:H1 (strain CFT073 / ATCC 700928 / UPEC)]
Length=294
Score = 133 bits (335), Expect = 2e-40
Identities = 87/281 (31%), Positives = 145/281 (52%), Gaps = 9/281 (3%)
Query 1 MRIGFIGLGAMGWHMAGHLTQLDATVMVWNRTQHKAEAHAMQFGSLAVELEQLV--QADL 58
M++GFIGLG MG M+ +L + +++V +R +A A + G+ + + Q D+
Sbjct 1 MKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNP-EAIADVIAAGAETASTAKAIAEQCDV 59
Query 59 IFSCLPTSAEVEAVIAAYP-----PRSGSVWIDCTSGVPASAQQLSRHLKQHGVDFLDAP 113
I + LP S V+ V + G+V ID +S P +++++S LK G+D LDAP
Sbjct 60 IITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLASREISEALKAKGIDMLDAP 119
Query 114 VSGQTIGAEKGTLTVMIGGERTAFERAQPIIQAFAGLIEHVGDSGAGFAVKAVNNILMAA 173
VSG A GTL+VM+GG++ F++ +++A AG + H G+ GAG K N +++A
Sbjct 120 VSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVVHTGEIGAGNVTKLANQVIVAL 179
Query 174 NLWALSEGMSVLKAHAVHLDAALACINHSSGKSTMSETIMPQKILSRGFHKTFALNLLQK 233
N+ A+SE +++ V+ D I ST+ + P ++ R F F ++L K
Sbjct 180 NIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLDAKAPM-VMDRNFKPGFRIDLHIK 238
Query 234 DIGIAIDLIKAVGQPADVLHCVQQMYSEMDLERATQVDFSA 274
D+ A+D VG + V +M + + D SA
Sbjct 239 DLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGTADHSA 279
>P0ABQ2 2-hydroxy-3-oxopropionate reductase [Escherichia coli
(strain K12)]
Length=294
Score = 133 bits (335), Expect = 2e-40
Identities = 87/281 (31%), Positives = 145/281 (52%), Gaps = 9/281 (3%)
Query 1 MRIGFIGLGAMGWHMAGHLTQLDATVMVWNRTQHKAEAHAMQFGSLAVELEQLV--QADL 58
M++GFIGLG MG M+ +L + +++V +R +A A + G+ + + Q D+
Sbjct 1 MKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNP-EAIADVIAAGAETASTAKAIAEQCDV 59
Query 59 IFSCLPTSAEVEAVIAAYP-----PRSGSVWIDCTSGVPASAQQLSRHLKQHGVDFLDAP 113
I + LP S V+ V + G+V ID +S P +++++S LK G+D LDAP
Sbjct 60 IITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLASREISEALKAKGIDMLDAP 119
Query 114 VSGQTIGAEKGTLTVMIGGERTAFERAQPIIQAFAGLIEHVGDSGAGFAVKAVNNILMAA 173
VSG A GTL+VM+GG++ F++ +++A AG + H G+ GAG K N +++A
Sbjct 120 VSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVVHTGEIGAGNVTKLANQVIVAL 179
Query 174 NLWALSEGMSVLKAHAVHLDAALACINHSSGKSTMSETIMPQKILSRGFHKTFALNLLQK 233
N+ A+SE +++ V+ D I ST+ + P ++ R F F ++L K
Sbjct 180 NIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLDAKAPM-VMDRNFKPGFRIDLHIK 238
Query 234 DIGIAIDLIKAVGQPADVLHCVQQMYSEMDLERATQVDFSA 274
D+ A+D VG + V +M + + D SA
Sbjct 239 DLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGTADHSA 279
>Q54CX6 Probable 3-hydroxyisobutyrate dehydrogenase, mitochondrial
[Dictyostelium discoideum]
Length=321
Score = 131 bits (329), Expect = 2e-39
Identities = 88/291 (30%), Positives = 145/291 (50%), Gaps = 12/291 (4%)
Query 3 IGFIGLGAMGWHMAGHLTQLDATVMVWNRTQHKAEAHAMQFGSLAVELEQLV-QADLIFS 61
+GFIGLG MG H A +L + ++V++ ++ + +A ++ +AD+I +
Sbjct 25 VGFIGLGNMGGHQAINLIKKGHNLIVFDMSKDNMNRLKEKGAKIANSPAEVAKEADVIVT 84
Query 62 CLPTSAEVEAVIAA-----YPPRSGSVWIDCTSGVPASAQQLSRHLKQHGVDFLDAPVSG 116
LP SA V+ V R G++ +D ++ PA+A++++ K+H LD PVSG
Sbjct 85 MLPASAHVKNVYCGENGIFQTVRPGTLLLDSSTIDPATAREVASIAKKHQSTMLDCPVSG 144
Query 117 QTIGAEKGTLTVMIGGERTAFERAQPIIQAFAGLIEHVGDSGAGFAVKAVNNILMAANLW 176
T GAE GTLT M+GG F A+ ++ I H GD G G K NN+++ ++
Sbjct 145 GTGGAEAGTLTFMVGGSEQDFNTAKTYLECMGKNIVHCGDVGTGQVAKVCNNLVLGISMI 204
Query 177 ALSEGMSVLKAHAVHLDAALACINHSSGKSTMSETIMP------QKILSRGFHKTFALNL 230
A+SE M++ + N SS + SE P SRG+ F L
Sbjct 205 AVSEAMNLGVKQGMDPKKLAGIFNTSSARCWTSELYNPCPGVIETSPASRGYTGGFGSAL 264
Query 231 LQKDIGIAIDLIKAVGQPADVLHCVQQMYSEMDLERATQVDFSAAIQTLEQ 281
+ KD+G+A+D K++G+P + + Q+Y+ + + Q DFS L +
Sbjct 265 MTKDLGLAVDSAKSIGEPLLLGNSAHQLYTLLVAKGDGQKDFSVVYDFLNK 315
>P77161 2-hydroxy-3-oxopropionate reductase [Escherichia coli
(strain K12)]
Length=292
Score = 127 bits (320), Expect = 2e-38
Identities = 89/290 (31%), Positives = 147/290 (51%), Gaps = 16/290 (6%)
Query 1 MRIGFIGLGAMGWHMAGHLTQLDATVMVWNRTQHKAEAHAMQFGSLAVELEQLVQ--ADL 58
M++GFIGLG MG MA +L + + V T + G+++VE + V +D+
Sbjct 1 MKLGFIGLGIMGTPMAINLARAGHQLHV--TTIGPVADELLSLGAVSVETARQVTEASDI 58
Query 59 IFSCLPTSAEVEAVI-----AAYPPRSGSVWIDCTSGVPASAQQLSRHLKQHGVDFLDAP 113
IF +P + +VE V+ G +D +S P ++ +R + + G D+LDAP
Sbjct 59 IFIMVPDTPQVEEVLFGENGCTKASLKGKTIVDMSSISPIETKRFARQVNELGGDYLDAP 118
Query 114 VSGQTIGAEKGTLTVMIGGERTAFERAQPIIQAFAGLIEHVGDSGAGFAVKAVNNILMAA 173
VSG IGA +GTL++M+GG+ FER +P+ + I VG +G G K N I++A
Sbjct 119 VSGGEIGAREGTLSIMVGGDEAVFERVKPLFELLGKNITLVGGNGDGQTCKVANQIIVAL 178
Query 174 NLWALSEGM---SVLKAHAVHLDAALACINHSSGKSTMSETIMPQKILSRGFHKTFALNL 230
N+ A+SE + S A V + AL S+ + ++++ R F+ F + L
Sbjct 179 NIEAVSEALLFASKAGADPVRVRQALM----GGFASSRILEVHGERMIKRTFNPGFKIAL 234
Query 231 LQKDIGIAIDLIKAVGQPADVLHCVQQMYSEMDLERATQVDFSAAIQTLE 280
QKD+ +A+ KA+ Q++++ +Q+D SA +Q LE
Sbjct 235 HQKDLNLALQSAKALALNLPNTATCQELFNTCAANGGSQLDHSALVQALE 284
>O34948 Uncharacterized oxidoreductase YkwC [Bacillus subtilis
(strain 168)]
Length=288
Score = 125 bits (315), Expect = 1e-37
Identities = 82/267 (31%), Positives = 140/267 (52%), Gaps = 9/267 (3%)
Query 3 IGFIGLGAMGWHMAGHLTQLDATVMVWNRTQHKAEAHAMQFGSLAVELEQLV--QADLIF 60
IGFIGLG MG MA H+ V+V+ RT+ KAE+ +Q G++ + + + +AD+I
Sbjct 5 IGFIGLGVMGKSMASHILNDGHPVLVYTRTKEKAES-ILQKGAIWKDTVKDLSKEADVII 63
Query 61 SCLPTSAEVEAVIAAYP-----PRSGSVWIDCTSGVPASAQQLSRHLKQHGVDFLDAPVS 115
+ + ++VE V + G+ ID T+ P+ A++++ K+ + LDAPVS
Sbjct 64 TMVGYPSDVEEVYFGSNGIIENAKEGAYLIDMTTSKPSLAKKIAEAAKEKALFALDAPVS 123
Query 116 GQTIGAEKGTLTVMIGGERTAFERAQPIIQAFAGLIEHVGDSGAGFAVKAVNNILMAANL 175
G IGA+ GTL +M+GGE+ AFE PI I++ G +G+G K N I +AA +
Sbjct 124 GGDIGAQNGTLAIMVGGEKEAFEACMPIFSLMGENIQYQGPAGSGQHTKMCNQIAIAAGM 183
Query 176 WALSEGMSVLKAHAVHLDAALACINHSSGKSTMSETIMPQKILSRGFHKTFALNLLQKDI 235
++E M+ + + + L I + S + P ++L F F + KD+
Sbjct 184 IGVAEAMAYAQKSGLEPENVLKSITTGAAGSWSLSNLAP-RMLQGNFEPGFYVKHFIKDM 242
Query 236 GIAIDLIKAVGQPADVLHCVQQMYSEM 262
GIA++ + +G+ L + +Y ++
Sbjct 243 GIALEEAELMGEEMPGLSLAKSLYDKL 269
>Q9I5I6 NAD-dependent L-serine dehydrogenase [Pseudomonas aeruginosa
(strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847
/ LMG 12228 / 1C / PRS 101 / PAO1)]
Length=298
Score = 122 bits (305), Expect = 4e-36
Identities = 87/286 (30%), Positives = 137/286 (48%), Gaps = 16/286 (6%)
Query 2 RIGFIGLGAMGWHMAGHLTQLDATVMVWNRTQHKAEAHAMQFGSLAVELEQLVQ-ADLIF 60
+I FIGLG MG MA +L + + V++ Q + S A VQ AD++
Sbjct 3 QIAFIGLGHMGAPMATNLLKAGYLLNVFDLVQSAVDGLVAAGASAARSARDAVQGADVVI 62
Query 61 SCLPTSAEVEAV-------IAAYPPRSGSVWIDCTSGVPASAQQLSRHLKQHGVDFLDAP 113
S LP S VE + +A P G++ ++C++ P SA+++ ++ G+ LDAP
Sbjct 63 SMLPASQHVEGLYLDDDGLLAHIAP--GTLVLECSTIAPTSARKIHAAARERGLAMLDAP 120
Query 114 VSGQTIGAEKGTLTVMIGGERTAFERAQPIIQAFAGLIEHVGDSGAGFAVKAVNNILMAA 173
VSG T GA GTLT M+GG+ A E+A+P+ +A I H G GAG K NN L+A
Sbjct 121 VSGGTAGAAAGTLTFMVGGDAEALEKARPLFEAMGRNIFHAGPDGAGQVAKVCNNQLLAV 180
Query 174 NLWALSEGMSVLKAHAVHLDAALACINHSSGKSTMSET------IMPQKILSRGFHKTFA 227
+ +E M++ A+ + + SSG + E +M SR + F
Sbjct 181 LMIGTAEAMALGVANGLEAKVLAEIMRRSSGGNWALEVYNPWPGVMENAPASRDYSGGFM 240
Query 228 LNLLQKDIGIAIDLIKAVGQPADVLHCVQQMYSEMDLERATQVDFS 273
L+ KD+G+A + +A + +Y + + + DFS
Sbjct 241 AQLMAKDLGLAQEAAQASASSTPMGSLALSLYRLLLKQGYAERDFS 286
>Q9SZE1 Probable 3-hydroxyisobutyrate dehydrogenase-like 1, mitochondrial
[Arabidopsis thaliana]
Length=334
Score = 120 bits (302), Expect = 2e-35
Identities = 87/293 (30%), Positives = 144/293 (49%), Gaps = 13/293 (4%)
Query 2 RIGFIGLGAMGWHMAGHLTQLDATVMVWNRTQHKAEAHAMQFGSLAVELEQLV--QADLI 59
+IG+IG G MG M GHL + TV V+NRT KA+ + G+ + V Q+D++
Sbjct 39 KIGWIGTGVMGRSMCGHLIKAGYTVTVFNRTISKAQT-LIDMGANVADSPNSVAEQSDVV 97
Query 60 FSCLPTSAEVEAVI------AAYPPRSGSVWIDCTSGVPASAQQLSRHLKQHGVDFLDAP 113
F+ + ++V V+ A R G V +D T+ P+ A+++++ +DAP
Sbjct 98 FTIVGYPSDVRHVLLDPKSGALSGLRQGGVLVDMTTSEPSLAEEIAKAASFKNCFSIDAP 157
Query 114 VSGQTIGAEKGTLTVMIGGERTAFERAQPIIQAFAGLIEHVGDSGAGFAVKAVNNILMAA 173
VSG +GA+ G L++ GG+ T +R P+ + G + +G SG G K N I +A+
Sbjct 158 VSGGDLGAKNGKLSIFAGGDETTVKRLDPLF-SLMGKVNFMGTSGKGQFAKLANQITIAS 216
Query 174 NLWALSEGMSVLKAHAVHLDAALACINHSSGKS-TMSETIMPQKILSRGFHKTFALNLLQ 232
+ L EG+ + AH LD S+G + + S + +IL R F F +N
Sbjct 217 TMLGLVEGL--IYAHKAGLDVKKFLEAISTGAAGSKSIDLYGDRILKRDFDPGFYVNHFV 274
Query 233 KDIGIAIDLIKAVGQPADVLHCVQQMYSEMDLERATQVDFSAAIQTLEQRNQL 285
KD+GI ++ + +G L QQ+Y + + A + LE+ N +
Sbjct 275 KDLGICLNECQRMGLALPGLALAQQLYLSLKAHGEGDLGTQALLLALERLNNV 327
>P9WNY5 Probable 3-hydroxyisobutyrate dehydrogenase [Mycobacterium
tuberculosis (strain ATCC 25618 / H37Rv)]
Length=294
Score = 116 bits (291), Expect = 4e-34
Identities = 87/293 (30%), Positives = 142/293 (48%), Gaps = 18/293 (6%)
Query 3 IGFIGLGAMGWHMAGHLTQLDATVMVWNRTQHKAE---AHAMQFGSLAVELEQLVQADLI 59
I F+GLG MG M+ +L V ++ A AH + A E + +AD++
Sbjct 4 IAFLGLGNMGAPMSANLVGAGHVVRGFDPAPTAASGAAAHGVAVFRSAPEA--VAEADVV 61
Query 60 FSCLPTSAEVEA----VIAAYPPRSGSVWIDCTSGVPASAQQLSRHLKQHGVDFLDAPVS 115
+ LPT V V+AA P +++ID ++ A+++ + HG+ LDAPVS
Sbjct 62 ITMLPTGEVVRRCYTDVLAAARP--ATLFIDSSTISVTDAREVHALAESHGMLQLDAPVS 119
Query 116 GQTIGAEKGTLTVMIGGERTAFERAQPIIQAFAGLIEHVGDSGAGFAVKAVNNILMAANL 175
G GA TL M+GG+ + RA+P+++ AG I H G +GAG A K NN+++A
Sbjct 120 GGVKGAAAATLAFMVGGDESTLRRARPVLEPMAGKIIHCGAAGAGQAAKVCNNMVLAVQQ 179
Query 176 WALSEGMSVLKAHAVHLDAALACINHSSGKSTMSET------IMPQKILSRGFHKTFALN 229
A++E + + + + I ++G T +P + F F+
Sbjct 180 IAIAEAFVLAEKLGLSAQSLFDVITGATGNCWAVHTNCPVPGPVPTSPANNDFKPGFSTA 239
Query 230 LLQKDIGIAIDLIKAVGQPADVLHCVQQMYSEMDLERATQVDFSAAIQTLEQR 282
L+ KD+G+A+D + A G A + +Y++ + A +DFSA I TL R
Sbjct 240 LMNKDLGLAMDAVAATGATAPLGSHAADIYAKFAADHA-DLDFSAVIHTLRAR 291
>P9WNY4 Probable 3-hydroxyisobutyrate dehydrogenase [Mycobacterium
tuberculosis (strain CDC 1551 / Oshkosh)]
Length=294
Score = 116 bits (291), Expect = 4e-34
Identities = 87/293 (30%), Positives = 142/293 (48%), Gaps = 18/293 (6%)
Query 3 IGFIGLGAMGWHMAGHLTQLDATVMVWNRTQHKAE---AHAMQFGSLAVELEQLVQADLI 59
I F+GLG MG M+ +L V ++ A AH + A E + +AD++
Sbjct 4 IAFLGLGNMGAPMSANLVGAGHVVRGFDPAPTAASGAAAHGVAVFRSAPEA--VAEADVV 61
Query 60 FSCLPTSAEVEA----VIAAYPPRSGSVWIDCTSGVPASAQQLSRHLKQHGVDFLDAPVS 115
+ LPT V V+AA P +++ID ++ A+++ + HG+ LDAPVS
Sbjct 62 ITMLPTGEVVRRCYTDVLAAARP--ATLFIDSSTISVTDAREVHALAESHGMLQLDAPVS 119
Query 116 GQTIGAEKGTLTVMIGGERTAFERAQPIIQAFAGLIEHVGDSGAGFAVKAVNNILMAANL 175
G GA TL M+GG+ + RA+P+++ AG I H G +GAG A K NN+++A
Sbjct 120 GGVKGAAAATLAFMVGGDESTLRRARPVLEPMAGKIIHCGAAGAGQAAKVCNNMVLAVQQ 179
Query 176 WALSEGMSVLKAHAVHLDAALACINHSSGKSTMSET------IMPQKILSRGFHKTFALN 229
A++E + + + + I ++G T +P + F F+
Sbjct 180 IAIAEAFVLAEKLGLSAQSLFDVITGATGNCWAVHTNCPVPGPVPTSPANNDFKPGFSTA 239
Query 230 LLQKDIGIAIDLIKAVGQPADVLHCVQQMYSEMDLERATQVDFSAAIQTLEQR 282
L+ KD+G+A+D + A G A + +Y++ + A +DFSA I TL R
Sbjct 240 LMNKDLGLAMDAVAATGATAPLGSHAADIYAKFAADHA-DLDFSAVIHTLRAR 291
>P63936 Probable 3-hydroxyisobutyrate dehydrogenase [Mycobacterium
bovis (strain ATCC BAA-935 / AF2122/97)]
Length=294
Score = 116 bits (291), Expect = 4e-34
Identities = 87/293 (30%), Positives = 142/293 (48%), Gaps = 18/293 (6%)
Query 3 IGFIGLGAMGWHMAGHLTQLDATVMVWNRTQHKAE---AHAMQFGSLAVELEQLVQADLI 59
I F+GLG MG M+ +L V ++ A AH + A E + +AD++
Sbjct 4 IAFLGLGNMGAPMSANLVGAGHVVRGFDPAPTAASGAAAHGVAVFRSAPEA--VAEADVV 61
Query 60 FSCLPTSAEVEA----VIAAYPPRSGSVWIDCTSGVPASAQQLSRHLKQHGVDFLDAPVS 115
+ LPT V V+AA P +++ID ++ A+++ + HG+ LDAPVS
Sbjct 62 ITMLPTGEVVRRCYTDVLAAARP--ATLFIDSSTISVTDAREVHALAESHGMLQLDAPVS 119
Query 116 GQTIGAEKGTLTVMIGGERTAFERAQPIIQAFAGLIEHVGDSGAGFAVKAVNNILMAANL 175
G GA TL M+GG+ + RA+P+++ AG I H G +GAG A K NN+++A
Sbjct 120 GGVKGAAAATLAFMVGGDESTLRRARPVLEPMAGKIIHCGAAGAGQAAKVCNNMVLAVQQ 179
Query 176 WALSEGMSVLKAHAVHLDAALACINHSSGKSTMSET------IMPQKILSRGFHKTFALN 229
A++E + + + + I ++G T +P + F F+
Sbjct 180 IAIAEAFVLAEKLGLSAQSLFDVITGATGNCWAVHTNCPVPGPVPTSPANNDFKPGFSTA 239
Query 230 LLQKDIGIAIDLIKAVGQPADVLHCVQQMYSEMDLERATQVDFSAAIQTLEQR 282
L+ KD+G+A+D + A G A + +Y++ + A +DFSA I TL R
Sbjct 240 LMNKDLGLAMDAVAATGATAPLGSHAADIYAKFAADHA-DLDFSAVIHTLRAR 291
>Q9V8M5 Probable 3-hydroxyisobutyrate dehydrogenase, mitochondrial
[Drosophila melanogaster]
Length=324
Score = 115 bits (287), Expect = 3e-33
Identities = 75/289 (26%), Positives = 141/289 (49%), Gaps = 10/289 (3%)
Query 3 IGFIGLGAMGWHMAGHLTQLDATVMVWNRTQHKAEAHAMQFGSLAVELEQLVQ-ADLIFS 61
IGF+GLG MG +MA +L + + V++ ++ + A + ++ + +L + +D + +
Sbjct 31 IGFVGLGNMGANMASNLIKAGHKLHVFDISKPACDGLAAKGATVYAKTSELAKNSDFVIT 90
Query 62 CLPTSAEVEAV---IAAYPPRSGSVWIDCTSGVPASAQQLSRHLKQHGVDFLDAPVSGQT 118
LP +A V+A + A +++ID ++ P + L + + G F+DAPVSG
Sbjct 91 MLPNNAIVDASYDEMTADGVNKDTIFIDSSTISPDLVKSLQKKISAKGARFIDAPVSGGV 150
Query 119 IGAEKGTLTVMIGGERTAFERAQPIIQAFAGLIEHVGDSGAGFAVKAVNNILMAANLWAL 178
GAE+ TLT M+GG + + +++ I H G G G A K NN+++A ++ +
Sbjct 151 PGAEQATLTFMVGGTEAEYNAVKAVLECMGKKITHCGVYGMGQAAKLCNNMMLAISMIGV 210
Query 179 SEGMSVLKAHAVHLDAALACINHSSGKSTMSET------IMPQKILSRGFHKTFALNLLQ 232
SE M++ + + IN S+G+ SE + P +R + F+ L+
Sbjct 211 SEAMNLAVRQGLDANVFAEIINSSTGRCWASEIYNPVPGVCPSAPANRDYAGGFSSALIT 270
Query 233 KDIGIAIDLIKAVGQPADVLHCVQQMYSEMDLERATQVDFSAAIQTLEQ 281
KD+G+A + A P + ++Y + + DFS +++
Sbjct 271 KDLGLASGVANASNSPIPLGSLAHKVYQSLCDKGLGNKDFSVVYDLMKK 319
>F4I907 Glyoxylate/succinic semialdehyde reductase 2, chloroplastic
[Arabidopsis thaliana]
Length=358
Score = 106 bits (264), Expect = 9e-30
Identities = 79/287 (28%), Positives = 138/287 (48%), Gaps = 13/287 (5%)
Query 3 IGFIGLGAMGWHMAGHLTQLDATVMVWNRTQHKAEAHAMQFGSLAVELEQLVQA-DLIFS 61
IGF+G+G MG MA +L + V VWNRT+ K + E++ DL F+
Sbjct 69 IGFLGMGIMGSPMAQNLIKAGCDVTVWNRTKSKCDPLVGLGAKYKSSPEEVTATCDLTFA 128
Query 62 CLPTSAEVEAVI--------AAYPPRSGSVWIDCTSGVPASAQQLSRHLKQHGVDFLDAP 113
L A+ E+ I A + SG ++D ++ AS+ +S+ +K G FL+AP
Sbjct 129 ML---ADPESAIDVACGKNGAIFGISSGKGYVDVSTVDVASSILISKQIKDTGALFLEAP 185
Query 114 VSGQTIGAEKGTLTVMIGGERTAFERAQPIIQAFAGLIEHVGDSGAGFAVKAVNNILMAA 173
VSG AE G L + G++ +E+A P + ++G+ G G A+K V N++M +
Sbjct 186 VSGSKKPAEDGQLIFLTAGDKPLYEKAAPFLDIMGKSKFYLGEVGNGAAMKLVVNMIMGS 245
Query 174 NLWALSEGMSVLKAHAVHLDAALACINHSSGKSTMSETIMPQKILSRGFHKTFALNLLQK 233
+ + +EG+ + + + + + ++ + + M P I S + F L QK
Sbjct 246 MMASFAEGILLSQKVGLDPNVLVEVVSQGAINAPMYSLKGPSMIKS-VYPTAFPLKHQQK 304
Query 234 DIGIAIDLIKAVGQPADVLHCVQQMYSEMDLERATQVDFSAAIQTLE 280
D+ +A+ L ++V Q + ++Y + DFSA I+ L+
Sbjct 305 DMRLALGLAESVSQSTPIAAAANELYKVAKSYGLSDEDFSAVIEALK 351
>P28811 3-hydroxyisobutyrate dehydrogenase [Pseudomonas aeruginosa
(strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847
/ LMG 12228 / 1C / PRS 101 / PAO1)]
Length=298
Score = 105 bits (261), Expect = 1e-29
Identities = 77/261 (30%), Positives = 128/261 (49%), Gaps = 24/261 (9%)
Query 3 IGFIGLGAMGWHMAGHLTQLDATVMVWNRTQHKAEAHAMQFGSLAVE--LEQLVQADLIF 60
I F+GLG MG MA +L + V V++ Q KA ++ G+ + L+ A+++
Sbjct 4 IAFLGLGNMGGPMAANLLKAGHRVNVFD-LQPKAVLGLVEQGAQGADSALQCCEGAEVVI 62
Query 61 SCLPTSAEVEAV----------IAAYPPRSGSVWIDCTSGVPASAQQLSRHLKQHGVDFL 110
S LP VE++ +A P + IDC++ P +A++++ G+ L
Sbjct 63 SMLPAGQHVESLYLGDDGLLARVAGKP-----LLIDCSTIAPETARKVAEAAAAKGLTLL 117
Query 111 DAPVSGQTIGAEKGTLTVMIGGERTAFERAQPIIQAFAGLIEHVGDSGAGFAVKAVNNIL 170
DAPVSG GA GTL+ ++GG F RA+P+++ I H GD GAG K NN+L
Sbjct 118 DAPVSGGVGGARAGTLSFIVGGPAEGFARARPVLENMGRNIFHAGDHGAGQVAKICNNML 177
Query 171 MAANLWALSEGMSVLKAHAVHLDAALACINHSSGKSTMSET------IMPQKILSRGFHK 224
+ + +E +++ + + + SSG + +MPQ S G+
Sbjct 178 LGILMAGTAEALALGVKNGLDPAVLSEVMKQSSGGNWALNLYNPWPGVMPQAPASNGYAG 237
Query 225 TFALNLLQKDIGIAIDLIKAV 245
F + L+ KD+G+A+ +AV
Sbjct 238 GFQVRLMNKDLGLALANAQAV 258
>P0A9V8 3-sulfolactaldehyde reductase [Escherichia coli (strain
K12)]
Length=298
Score = 95.5 bits (236), Expect = 3e-26
Identities = 79/283 (28%), Positives = 131/283 (46%), Gaps = 10/283 (4%)
Query 3 IGFIGLGAMGWHMAGHLTQLDATVMVWNRTQHKAEAHAMQFGSL--AVELEQLVQADLIF 60
I FIGLG MG MA +L Q + V++ +A H + G+ A + A+ I
Sbjct 4 IAFIGLGQMGSPMASNLLQQGHQLRVFD-VNAEAVRHLVDKGATPAANPAQAAKDAEFII 62
Query 61 SCLPTSAEVEAVI-----AAYPPRSGSVWIDCTSGVPASAQQLSRHLKQHGVDFLDAPVS 115
+ LP V V+ + ++ ID ++ P +L ++ G +D PV
Sbjct 63 TMLPNGDLVRNVLFGENGVCEGLSTDALVIDMSTIHPLQTDKLIADMQAKGFSMMDVPVG 122
Query 116 GQTIGAEKGTLTVMIGGERTAFERAQPIIQAFAGLIEHVGDSGAGFAVKAVNNILMAANL 175
+ A GTL ++ GG ERA PI+ A + + G G G VK +NN + A L
Sbjct 123 RTSANAITGTLLLLAGGTAEQVERATPILMAMGSELINAGGPGMGIRVKLINNYMSIA-L 181
Query 176 WALSEGMSVL-KAHAVHLDAALACINHSSGKSTMSETIMPQKILSRGFHKTFALNLLQKD 234
ALS +VL +A + D A+ ++ ++ T P K+LS F ++L KD
Sbjct 182 NALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTTSWPNKVLSGDLSPAFMIDLAHKD 241
Query 235 IGIAIDLIKAVGQPADVLHCVQQMYSEMDLERATQVDFSAAIQ 277
+GIA+D+ + P + +++YS+ + D+SA ++
Sbjct 242 LGIALDVANQLHVPMPLGAASREVYSQARAAGRGRQDWSAILE 284
>Q49A26 Cytokine-like nuclear factor N-PAC [Homo sapiens]
Length=553
Score = 94.4 bits (233), Expect = 6e-25
Identities = 68/279 (24%), Positives = 122/279 (44%), Gaps = 7/279 (3%)
Query 2 RIGFIGLGAMGWHMAGHLTQLDATVMVWNRTQHKAEAHAMQFGSLAVELEQLVQA-DLIF 60
+IGF+GLG MG + +L ++ TV VWNRT K + + L ++V D+ F
Sbjct 269 KIGFLGLGLMGSGIVSNLLKMGHTVTVWNRTAEKCDLFIQEGARLGRTPAEVVSTCDITF 328
Query 61 SCLPTSAEVEAVIAAYPP-----RSGSVWIDCTSGVPASAQQLSRHLKQHGVDFLDAPVS 115
+C+ + ++ R G ++D ++ + +L++ + G FL+APVS
Sbjct 329 ACVSDPKAAKDLVLGPSGVLQGIRPGKCYVDMSTVDADTVTELAQVIVSRGGRFLEAPVS 388
Query 116 GQTIGAEKGTLTVMIGGERTAFERAQPIIQAFAGLIEHVGDSGAGFAVKAVNNILMAANL 175
G + G L ++ G+R +E QA +G+ G + + N++ + +
Sbjct 389 GNQQLSNDGMLVILAAGDRGLYEDCSSCFQAMGKTSFFLGEVGNAAKMMLIVNMVQGSFM 448
Query 176 WALSEGMSVLKAHAVHLDAALACINHSSGKSTMSETIMPQKILSRGFHKTFALNLLQKDI 235
++EG+++ + L +N S + Q IL F F L +QKD+
Sbjct 449 ATIAEGLTLAQVTGQSQQTLLDILNQGQLASIFLDQ-KCQNILQGNFKPDFYLKYIQKDL 507
Query 236 GIAIDLIKAVGQPADVLHCVQQMYSEMDLERATQVDFSA 274
+AI L AV P + ++Y + D SA
Sbjct 508 RLAIALGDAVNHPTPMAAAANEVYKRAKALDQSDNDMSA 546
>Q922P9 Cytokine-like nuclear factor N-PAC [Mus musculus]
Length=546
Score = 92.8 bits (229), Expect = 2e-24
Identities = 69/278 (25%), Positives = 122/278 (44%), Gaps = 11/278 (4%)
Query 2 RIGFIGLGAMGWHMAGHLTQLDATVMVWNRTQHKAEAHAMQFGSLAVELEQLVQADLIFS 61
+IGF+GLG MG + +L ++ TV VWNRT AE + G E+ + D+ F+
Sbjct 268 KIGFLGLGLMGSGIVSNLLKMGHTVTVWNRT---AEKEGARLGRTPAEV--VSTCDITFA 322
Query 62 CLPTSAEVEAVIAAYPP-----RSGSVWIDCTSGVPASAQQLSRHLKQHGVDFLDAPVSG 116
C+ + ++ R G ++D ++ + +L++ + G FL+APVSG
Sbjct 323 CVSDPKAAKDLVLGPSGVLQGIRPGKCYVDMSTVDADTVTELAQVIVSRGGRFLEAPVSG 382
Query 117 QTIGAEKGTLTVMIGGERTAFERAQPIIQAFAGLIEHVGDSGAGFAVKAVNNILMAANLW 176
+ G L ++ G+R +E QA +G+ G + + N++ + +
Sbjct 383 NQQLSNDGMLVILAAGDRGLYEDCSSCFQAMGKTSFFLGEVGNAAKMMLIVNMVQGSFMA 442
Query 177 ALSEGMSVLKAHAVHLDAALACINHSSGKSTMSETIMPQKILSRGFHKTFALNLLQKDIG 236
++EG+++ + L +N S + Q IL F F L +QKD+
Sbjct 443 TIAEGLTLAQVTGQSQQTLLDILNQGQLASIFLDQ-KCQNILQGNFKPDFYLKYIQKDLR 501
Query 237 IAIDLIKAVGQPADVLHCVQQMYSEMDLERATQVDFSA 274
+AI L AV P + ++Y + D SA
Sbjct 502 LAIALGDAVNHPTPMAAAANEVYKRAKALDQSDNDMSA 539
Lambda K H a alpha
0.323 0.134 0.393 0.792 4.96
Gapped
Lambda K H a alpha sigma
0.267 0.0410 0.140 1.90 42.6 43.6
Effective search space used: 1250744
Database: c70766d9fe65f69657b4b8a63a21bbdf.SwissProt.fasta
Posted date: Jun 2, 2024 1:32 AM
Number of letters in database: 5,972
Number of sequences in database: 18
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 11
Window for multiple hits: 40
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Data ...
