BLASTP 2.12.0+
Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics: Alejandro A. Schaffer,
L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri
I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids
Res. 29:2994-3005.
Database: 0bc3461723e75f4e0db7b86576c541ef.SwissProt.fasta
10 sequences; 8,011 total letters
Query= ACIAD0167
Length=1107
Score E
Sequences producing significant alignments: (Bits) Value
Q9I6M7 Type VI secretion system spike protein VgrG2b [Pseudomonas... 183 2e-50
Q9I3K1 Type VI secretion system spike protein VgrG2a [Pseudomonas... 182 3e-50
Q9I741 Type VI secretion system spike protein VgrG1a [Pseudomonas... 142 4e-38
Q9KN42 Type VI secretion system spike protein VgrG3 [Vibrio chole... 132 3e-34
Q9KNE7 Type VI secretion system spike protein VgrG2 [Vibrio chole... 125 2e-32
A0A0H3AIG7 Actin cross-linking toxin VgrG1 [Vibrio cholerae serot... 125 3e-32
E0SIS4 Putative type VI secretion system protein VgrGB [Dickeya d... 123 5e-32
E0SAL0 Putative type VI secretion system protein VgrGA [Dickeya d... 117 5e-30
Q9I0F3 Type VI secretion system spike protein VgrG1c [Pseudomonas... 102 2e-25
Q9I737 Type VI secretion system spike protein VgrG1b [Pseudomonas... 102 2e-25
>Q9I6M7 Type VI secretion system spike protein VgrG2b [Pseudomonas
aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116
/ JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)]
Length=1019
Score = 183 bits (465), Expect = 2e-50
Identities = 121/327 (37%), Positives = 168/327 (51%), Gaps = 47/327 (14%)
Query 351 GYWFELQGHPEIDQHDLADRQFLIYEKRFFNQNNLPKDIQQQFEYLLMHSALKTSNQQER 410
G+ F L+GHP D FN P + +H + + Q+E
Sbjct 299 GHAFALEGHPRAD----------------FNAWWRPVRV--------VHRGTQYAGQEEE 334
Query 411 QGNELTLIRRQVK--VVPEY-----QPLMHRPVVYPQRARVVGPENEEIYVDEWGRIKVR 463
+ + ++ +VPE PL + PQ A VVGP EEI+ DEWGR+KV+
Sbjct 335 SADAPLGVSYDLRAELVPEDVEWRPAPLPRPRIDGPQIATVVGPAGEEIHCDEWGRVKVQ 394
Query 464 FLFTRTEDHAHDGGAGSNDNDTDSAWVDVLTPWAGEGYGARFLPRVGELVVIDFFDGHVD 523
F + R H ++ + W+ V WAG +G +PR+G+ V++D+ DG D
Sbjct 395 FPWDREGRH----------DEFSTCWIRVAQNWAGADWGHMAIPRIGQEVIVDYLDGDCD 444
Query 524 RPFATGRIHEAQRSPTKFDIRGQLPATRNLSGIRSQEIAGSGFNQLRFDDTTGQISTQLQ 583
+P TGR + A P LP + LS I+S+E GS N+LR DDTT QIS L
Sbjct 445 QPIVTGRTYRATNRPPY-----ALPDHKILSTIKSKEYKGSRANELRIDDTTAQISAALM 499
Query 584 SSHGASQLNLGNLSHPKNEETSQGRGEGFELRTDHYGAVRAGKGMLISTYAQEQAIADHL 643
S HGAS L+LG L+HP+ E + RGEGFELRTD +GAVRA KG+L+ST Q +A A HL
Sbjct 500 SDHGASALHLGYLTHPR-PEGGKPRGEGFELRTDEHGAVRAAKGLLLSTEEQLRAGAGHL 558
Query 644 EAAQAQSLLAQSHENMKTLSDIALKQQ 670
+ +L + E + L D A + Q
Sbjct 559 DRGVVVQVLEAALELARELGDYAGEHQ 585
Score = 65.1 bits (157), Expect = 1e-13
Identities = 34/144 (24%), Positives = 75/144 (52%), Gaps = 0/144 (0%)
Query 861 LASPNGIALTTPENIILQASQDIAESASGSINLSAQKNIIGHAQDKVSLFAAQKGLSAYA 920
L+ P GIA TP ++ L A + + A + ++A + ++ +A + LFA L
Sbjct 624 LSGPAGIAAATPASLTLAAGEHVDSVARQNQQVTAGQKVVINAGSDIGLFAQGGELRQIT 683
Query 921 AKGAVKIQAQDDLVDIIAKKVIKLISTEDKIELTSTKEIDIKAGGSQLIVNGNGVFIKTG 980
+G + +QAQ + + + AK+ +++ +++ + +T+ + I + GG+ L + G + +
Sbjct 684 HQGPMLLQAQKNDIRLEAKQSVEVSASQQHVLVTAKEHITLMCGGAYLTLKGGNIELGMP 743
Query 981 GKFEVRSGQHVFTSGEKVSYEVPE 1004
G F V++ +H + E+P+
Sbjct 744 GNFVVKAAKHSHVGAASLEAELPQ 767
Score = 35.8 bits (81), Expect = 1e-04
Identities = 30/103 (29%), Positives = 45/103 (44%), Gaps = 11/103 (11%)
Query 88 GIITRATQGQSDGALTLYTLNIQDSTALWHKRRNSRVFMNKSVAEISEILFKEWQNRSSL 147
GI++ TQG S T Y L ++ A N R+F KSV EI + L KE +
Sbjct 73 GIVSHFTQGSSGFRRTRYELLLEPQLARLELCCNWRIFQEKSVPEILQALLKEHR----- 127
Query 148 FAASLTLDLSGLAQNYDI-RPFTMQINETDYEFLTRLWRSEGI 189
LD + + R + +Q ++D+ RL EG+
Sbjct 128 -----VLDYEQRIYHEHLPREYCVQAGDSDHYLHDRLAFEEGL 165
Score = 23.1 bits (48), Expect = 0.87
Identities = 12/35 (34%), Positives = 23/35 (66%), Gaps = 1/35 (3%)
Query 354 FELQGHPEIDQHDLADRQFLIYEKRFFNQNNLPKD 388
F+ + PEI Q L + + L YE+R +++ +LP++
Sbjct 110 FQEKSVPEILQALLKEHRVLDYEQRIYHE-HLPRE 143
Score = 20.0 bits (40), Expect = 7.6
Identities = 8/34 (24%), Positives = 18/34 (53%), Gaps = 0/34 (0%)
Query 36 LQRIDGKHTLNEGLEVELTCLSINAHIALKQFIG 69
+ ++D KHT + V ++ A +A+ + +G
Sbjct 823 IAKVDMKHTKSPASAVAGIAAAVGAAVAVGKLLG 856
>Q9I3K1 Type VI secretion system spike protein VgrG2a [Pseudomonas
aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116
/ JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)]
Length=842
Score = 182 bits (461), Expect = 3e-50
Identities = 120/327 (37%), Positives = 168/327 (51%), Gaps = 47/327 (14%)
Query 351 GYWFELQGHPEIDQHDLADRQFLIYEKRFFNQNNLPKDIQQQFEYLLMHSALKTSNQQER 410
G+ F L+GHP D FN P + +H + + Q+E
Sbjct 299 GHAFALEGHPRAD----------------FNAWWRPVRV--------VHRGTQYAGQEEE 334
Query 411 QGNELTLIRRQVK--VVPEY-----QPLMHRPVVYPQRARVVGPENEEIYVDEWGRIKVR 463
+ + ++ +VPE PL + PQ A VVGP EEI+ DEWGR+KV+
Sbjct 335 SADAPLGVSYDLRAELVPEDVEWRPAPLPRPRIDGPQIATVVGPAGEEIHCDEWGRVKVQ 394
Query 464 FLFTRTEDHAHDGGAGSNDNDTDSAWVDVLTPWAGEGYGARFLPRVGELVVIDFFDGHVD 523
F + R H ++ + W+ V WAG +G +PR+G+ V++D+ DG D
Sbjct 395 FPWDREGRH----------DEFSTCWIRVAQNWAGADWGHMAIPRIGQEVIVDYLDGDCD 444
Query 524 RPFATGRIHEAQRSPTKFDIRGQLPATRNLSGIRSQEIAGSGFNQLRFDDTTGQISTQLQ 583
+P TGR + A P LP + LS I+S+E GS N+LR DDTT QIS L
Sbjct 445 QPIVTGRTYRATNRPPY-----ALPDHKILSTIKSKEYKGSRANELRIDDTTAQISAALM 499
Query 584 SSHGASQLNLGNLSHPKNEETSQGRGEGFELRTDHYGAVRAGKGMLISTYAQEQAIADHL 643
S HGAS L+LG L+HP+ E + RGEGFELRTD +GAVRA KG+L+ST Q +A A HL
Sbjct 500 SDHGASALHLGYLTHPR-PEGGKPRGEGFELRTDEHGAVRAAKGLLLSTEEQLRAGAGHL 558
Query 644 EAAQAQSLLAQSHENMKTLSDIALKQQ 670
+ +L + + + L D A + Q
Sbjct 559 DRGVVVQVLEAALKLARELGDYAGEHQ 585
Score = 59.7 bits (143), Expect = 5e-12
Identities = 31/130 (24%), Positives = 70/130 (54%), Gaps = 0/130 (0%)
Query 861 LASPNGIALTTPENIILQASQDIAESASGSINLSAQKNIIGHAQDKVSLFAAQKGLSAYA 920
L+ P GIA TP ++ L A + + A + ++A + ++ +A + LFA L
Sbjct 624 LSGPAGIAAATPASLTLAAGEHVDSVARQNQQVTAGQKVVINAGSDIGLFAQGGELRQIT 683
Query 921 AKGAVKIQAQDDLVDIIAKKVIKLISTEDKIELTSTKEIDIKAGGSQLIVNGNGVFIKTG 980
+G + +QAQ + + + A++ +++ +++ + +T+ + I + GG+ L + G + +
Sbjct 684 HQGPMLLQAQKNDIRLEAEQSVEVSASQQHVLVTAKEHITLMCGGAYLTLKGGNIELGMP 743
Query 981 GKFEVRSGQH 990
G F V++ +H
Sbjct 744 GNFVVKAAKH 753
Score = 35.8 bits (81), Expect = 1e-04
Identities = 30/103 (29%), Positives = 45/103 (44%), Gaps = 11/103 (11%)
Query 88 GIITRATQGQSDGALTLYTLNIQDSTALWHKRRNSRVFMNKSVAEISEILFKEWQNRSSL 147
GI++ TQG S T Y L ++ A N R+F KSV EI + L KE +
Sbjct 73 GIVSHFTQGSSGFRRTRYELLLEPQLARLELCCNWRIFQEKSVPEILQALLKEHR----- 127
Query 148 FAASLTLDLSGLAQNYDI-RPFTMQINETDYEFLTRLWRSEGI 189
LD + + R + +Q ++D+ RL EG+
Sbjct 128 -----VLDYEQRIYHEHLPREYCVQAGDSDHYLHDRLAFEEGL 165
Score = 23.1 bits (48), Expect = 0.86
Identities = 12/35 (34%), Positives = 23/35 (66%), Gaps = 1/35 (3%)
Query 354 FELQGHPEIDQHDLADRQFLIYEKRFFNQNNLPKD 388
F+ + PEI Q L + + L YE+R +++ +LP++
Sbjct 110 FQEKSVPEILQALLKEHRVLDYEQRIYHE-HLPRE 143
>Q9I741 Type VI secretion system spike protein VgrG1a [Pseudomonas
aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116
/ JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)]
Length=643
Score = 142 bits (358), Expect = 4e-38
Identities = 159/661 (24%), Positives = 281/661 (43%), Gaps = 80/661 (12%)
Query 23 VQFSNPLLNTQIFLQRIDGKHTLNEGLEVELTCLSINAHIALKQFIGCQVAIDQVTDTGQ 82
VQ PL + LQR++G+ L EL +S N ++ L+Q +G +++ G
Sbjct 7 VQVDCPLGPDVLLLQRMEGREELGRLFAYELHLVSENPNLPLEQLLGKPMSLSLELPGGS 66
Query 83 LARTSGIITRATQGQSDGALTLYTLNIQDSTALWHKRRNSRVFMNKSVAEISEILFKEWQ 142
GI+ R +Q G Y ++ L + + R+F N+SV EI + +F+
Sbjct 67 RRFFHGIVARCSQVAGHGQFAGYQATLRPWPWLLTRTSDCRIFQNQSVPEIIKQVFRNLG 126
Query 143 NRSSLFAASLTLDLSGLAQNYDIRPFTMQINETDYEFLTRLWRSEGIN-WLVDEKNTVVT 201
S F +LT + Y + +Q ET ++F++RL EGI W E
Sbjct 127 --FSDFEDALT-------RPYREWEYCVQYRETSFDFISRLMEQEGIYYWFRHE------ 171
Query 202 HPLQPIAPQKLRLIDDNQQFLALSRGLIRFHRSHATEHFDSMTHLVAHRQLQSTAAYVQR 261
QK ++ L+ + G R +A+ + T + HR+ +
Sbjct 172 --------QKRHIL-----VLSDAYGAHRSPGGYASVPYYPPT--LGHRERD----HFFD 212
Query 262 WQADGLLQDEGSGSVLSAHQHSDQQSNESLSLEQAWSIGSAWTTDLTGADQATASSSQQI 321
WQ + ++ GS L+ + + Q+ L + + A SQ
Sbjct 213 WQ---MAREVQPGS-LTLNDYDFQRPGARLEVRSNIARPHAAADYPLYDYPGEYVQSQDG 268
Query 322 EKLNQNLTAYQERQAKYFIAQTTVRDAQVGYWFELQGHPEIDQHDLADRQFLIYEKRFFN 381
E+ +N + Q + + VR G+ F L G+P DQ +R++L+ +
Sbjct 269 EQYARNRIEAIQAQHERVRLRGVVRGIGAGHLFRLSGYPRDDQ----NREYLVVGAEY-- 322
Query 382 QNNLPKDIQQQFEYLLMHSALKTSNQQERQGNELTLIRRQVKVVPEYQPLMHRPVVY-PQ 440
+ +Q+ +E + + Q + + +++P+ P+ PVV PQ
Sbjct 323 -----RVVQELYE-----TGSGGAGSQFESELDCIDASQSFRLLPQ-TPV---PVVRGPQ 368
Query 441 RARVVGPENEEIYVDEWGRIKVRFLFTRTEDHAHDGGAGSNDNDTDSAWVDVLTPWAGEG 500
A VVGP+ EEI+ D++GR+KV F + R HD N+ S W+ V WAG+
Sbjct 369 TAVVVGPKGEEIWTDQYGRVKVHFHWDR-----HD-----QSNENSSCWIRVSQAWAGKN 418
Query 501 YGARFLPRVGELVVIDFFDGHVDRPFATGRIHEAQRSPTKFDIRGQLPATRNLSGIRSQE 560
+G+ +PR+G+ V++ F +G DRP TGR++ A+++ + +LPA SG++S+
Sbjct 419 WGSMQIPRIGQEVIVSFLEGDPDRPIITGRVYNAEQT-----VPYELPANATQSGMKSRS 473
Query 561 IAG---SGFNQLRFDDTTGQISTQLQSSHGASQL--NLGNLSHPKNEETSQGRGEGFELR 615
G + FN++R +D G + + L N +LS + S G E +
Sbjct 474 SKGGTPANFNEIRMEDKKGAEQLYIHAERNQDNLVENDASLSVGHDRNKSIGHDELARIG 533
Query 616 TDHYGAVRAGKGMLISTYAQEQAIADHLEAAQAQSLLAQSHENMKTLSDIALKQQTDALN 675
+ AV+ +L+ + +L A AQ L ++ +D + A N
Sbjct 534 NNRTRAVKLNDTLLVGGAKSDSVTGTYLIEAGAQIRLVCGKSVVEFNADGTINISGSAFN 593
Query 676 V 676
+
Sbjct 594 L 594
>Q9KN42 Type VI secretion system spike protein VgrG3 [Vibrio cholerae
serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)]
Length=1017
Score = 132 bits (331), Expect = 3e-34
Identities = 131/543 (24%), Positives = 217/543 (40%), Gaps = 100/543 (18%)
Query 48 GLEVELTCLSINAHIALKQFIGCQVAIDQVTDTGQLARTSGIITRATQGQSDGALTLYTL 107
G ++ S +++ +QF+ + + D + + +GI+ + ++G + T Y+L
Sbjct 41 GFRYQVDLASALSNLTAEQFVDQTAHLTILRDGQVVQQINGIVRQLSKGDTGHRHTFYSL 100
Query 108 NIQDSTALWHKRRNSRVFMNKSVAEISEILFKEWQNRSSLFAASLTLDLSGLAQNYDIRP 167
+ + R NSR+F +SV EI IL +E FA L + R
Sbjct 101 TLVPALERLSLRSNSRIFQQQSVPEIISILLQEMGIEDYAFA---------LKRECAQRE 151
Query 168 FTMQINETDYEFLTRLWRSEGINWL---VDEKNTVVTHPLQPIAPQKLRLIDDNQQFLAL 224
F +Q ETD +FL R+ EG+ + +K+T++ P+ + + N AL
Sbjct 152 FCVQYRETDLQFLHRIAAEEGLVYSHLHEAQKHTLLFTDSSDSQPKLAKPVPYN----AL 207
Query 225 SRGLIRFHRSHATEHFDSMTHLVAHRQLQSTA------AYVQRWQADGLLQDEGSGSVLS 278
+ G I + + T V+H +L+ + + QR Q + + +
Sbjct 208 AGGEINL--PYVVDLQFKTTAQVSHTELKDYSFKKPAYGFTQRTQGKDIAYQQPN----- 260
Query 279 AHQHSDQQSNESLSLEQAWSIGSAWTTDLTGADQATASSSQQIEKLNQNLTAYQERQAKY 338
++H D D A + QI Y R A
Sbjct 261 -YEHFDAPGRYK--------------------DDANGKAFSQIR------LEYLRRDALL 293
Query 339 FIAQTTVRDAQVGYWFELQGHPEIDQHDLADRQFLIYEKRFFNQNNLPKDIQQQFEYLLM 398
A++ G F+LQ H + +R +L+ +
Sbjct 294 ADAKSDEPLLLAGVRFDLQDHLD----HAMNRDWLVVQAN-------------------- 329
Query 399 HSALKTSNQQERQGNELTLIRRQVKVVPEYQPLMHRPVVYPQ-----RARVVGPENEEIY 453
H + QE G+ T Q+K++P + RP PQ A VVGP+ EEIY
Sbjct 330 HQGTQPQALQEEGGSGATTYSNQLKLIPAHITWRARPCAKPQVDGPMIATVVGPQGEEIY 389
Query 454 VDEWGRIKVRFLFTRTEDHAHDGGAGSNDNDTDSAWVDVLTPWAGEGYGARFLPRVGELV 513
D +GR+KV F + R S+ N+ S WV V WAG YG+ +PRVG V
Sbjct 390 CDNFGRVKVHFPWDRY----------SSSNEKSSCWVRVAQEWAGSQYGSMAIPRVGHEV 439
Query 514 VIDFFDGHVDRPFATGRIHEAQRSPTKFDIRGQLPATRNLSGIRSQEIAGSGFNQLRFDD 573
++ F +G D+P TGR + A + LP + + +R++ G G+N+L F+D
Sbjct 440 IVSFLNGDPDQPIITGRTYHATNTAPY-----ALPDHKTKTVLRTETHQGQGYNELSFED 494
Query 574 TTG 576
G
Sbjct 495 QAG 497
Score = 25.8 bits (55), Expect = 0.13
Identities = 11/34 (32%), Positives = 20/34 (59%), Gaps = 0/34 (0%)
Query 950 KIELTSTKEIDIKAGGSQLIVNGNGVFIKTGGKF 983
+I + + KEI +K+G ++ G + +K GG F
Sbjct 572 RIAVEAGKEISLKSGAKIVVEAGAELTLKAGGSF 605
Score = 23.5 bits (49), Expect = 0.67
Identities = 11/28 (39%), Positives = 18/28 (64%), Gaps = 0/28 (0%)
Query 950 KIELTSTKEIDIKAGGSQLIVNGNGVFI 977
KI + + E+ +KAGGS + V+ GV +
Sbjct 588 KIVVEAGAELTLKAGGSFVKVDAGGVHL 615
Score = 21.9 bits (45), Expect = 2.0
Identities = 11/43 (26%), Positives = 18/43 (42%), Gaps = 2/43 (5%)
Query 984 EVRSGQHVFTSGEKVSYEVPELPNTSIF--SNRLDIYDLFWAS 1024
E R QH F ++ L + NR ++DL W++
Sbjct 897 EFREEQHQFIKRTHYDIQIGHLRGKGLLFEHNRAAVHDLIWST 939
Score = 20.8 bits (42), Expect = 4.4
Identities = 13/43 (30%), Positives = 20/43 (47%), Gaps = 4/43 (9%)
Query 950 KIELTSTKEIDI----KAGGSQLIVNGNGVFIKTGGKFEVRSG 988
KI L S+ E+ K G + G + +K+G K V +G
Sbjct 552 KIALDSSHEVGASLQHKVGQRIAVEAGKEISLKSGAKIVVEAG 594
>Q9KNE7 Type VI secretion system spike protein VgrG2 [Vibrio cholerae
serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)]
Length=694
Score = 125 bits (313), Expect = 2e-32
Identities = 132/538 (25%), Positives = 214/538 (40%), Gaps = 88/538 (16%)
Query 48 GLEVELTCLSINAHIALKQFIGCQVAIDQVTDTGQLARTSGIITRATQGQSDGALTLYTL 107
G ++ S +++ Q + + + ++ + R GI+ +QG T Y L
Sbjct 41 GFRYQMQLASRVSNLTADQMVDKRAELKLYRNSQLVQRVHGIVRAFSQGDIGHHHTFYQL 100
Query 108 NIQDSTALWHKRRNSRVFMNKSVAEISEILFKEWQNRSSLFAASLTLDLSGLAQNYDIRP 167
+ + R NSR+F ++V EI IL +E FA L ++ R
Sbjct 101 TLVPALERLSLRHNSRIFQKQTVPEILSILLQEMGIHDYAFA---------LKRDCVQRE 151
Query 168 FTMQINETDYEFLTRLWRSEGINW-LVDE--KNTVVTHPLQPIAPQKLRLIDDNQQFLAL 224
F +Q E+D +FL RL EG+ + V E K+T+ A L + + + AL
Sbjct 152 FCVQYRESDIDFLHRLAAEEGLVYSFVHEAGKHTLYFSD----ASDSLSKLPEPIPYNAL 207
Query 225 SRGLIRFHRSHATEHFDSMTHLVAHRQLQSTAAYVQRWQADGLLQDEGSGSVLSAHQHSD 284
+ G + T + +T+ T A V Q + + S L Q ++
Sbjct 208 AGGTMD------TPYIHGLTY--------RTQAEVSEVQLKDYSFKKPAYSFLQTVQGTE 253
Query 285 QQSNESLSLEQAWSIGSAWTTDLTGADQATASSSQQIEKLNQNLTAYQERQAKYFIAQTT 344
++ Q + + D+ GA +Q Y R A Q+
Sbjct 254 LDYQQTRY--QHFDAPGRYKDDVNGA------------AFSQIRLDYLRRHAHTATGQSN 299
Query 345 VRDAQVGYWFELQGHPEIDQHDLADRQFLIYEKRFFNQNNLPKDIQQQFEYLLMHSALKT 404
+ GY F+LQ H + +R +++ + H +
Sbjct 300 EPLLRAGYKFDLQEHLD----PAMNRDWVVVS--------------------INHQGEQP 335
Query 405 SNQQERQGNELTLIRRQVKVVPEY-----QPLMHRPVVYPQRARVVGPENEEIYVDEWGR 459
QE G+ T Q ++P + +P V P A VVGPE EEI+ DE GR
Sbjct 336 QALQEEGGSGATTYNNQFSLIPGHLHWRAEPQPKPQVDGPMIATVVGPEGEEIFCDEHGR 395
Query 460 IKVRFLFTRTEDHAHDGGAGSNDNDTDSAWVDVLTPWAGEGYGARFLPRVGELVVIDFFD 519
+K+ F + R SN N+ S WV V WAG YG +PR+G V++ F +
Sbjct 396 VKIHFPWDRY----------SNGNEQSSCWVRVSQGWAGSQYGFIAIPRIGHEVIVSFLN 445
Query 520 GHVDRPFATGRIHEAQRSPTKFDIRGQLPATRNLSGIRSQEIAGSGFNQLRFDDTTGQ 577
G D+P TGR + A +P LP + + +R++ G GFN+L F+D G+
Sbjct 446 GDPDQPIITGRTYHATNTPPY-----TLPEHKTKTVLRTETHQGEGFNELSFEDQAGK 498
Score = 29.3 bits (64), Expect = 0.011
Identities = 20/58 (34%), Positives = 30/58 (52%), Gaps = 2/58 (3%)
Query 949 DKIELTSTKEIDIKAGGSQLIVNGNGVFIKTGGKFEVRSGQHVFTSGEKVSYEVPELP 1006
+K+ L + E+ IKAGGS + V+ GV + G + SG SG ++ ELP
Sbjct 587 NKLVLEAGNELTIKAGGSFIKVDAGGVHV-VGSAINLNSGGSA-GSGSGYGGKMAELP 642
Score = 26.6 bits (57), Expect = 0.073
Identities = 11/26 (42%), Positives = 16/26 (62%), Gaps = 0/26 (0%)
Query 958 EIDIKAGGSQLIVNGNGVFIKTGGKF 983
E+ +KAG ++ GN + IK GG F
Sbjct 580 EVHLKAGNKLVLEAGNELTIKAGGSF 605
>A0A0H3AIG7 Actin cross-linking toxin VgrG1 [Vibrio cholerae serotype
O1 (strain ATCC 39541 / Classical Ogawa 395 / O395)]
Length=1095
Score = 125 bits (315), Expect = 3e-32
Identities = 134/538 (25%), Positives = 215/538 (40%), Gaps = 88/538 (16%)
Query 48 GLEVELTCLSINAHIALKQFIGCQVAIDQVTDTGQLARTSGIITRATQGQSDGALTLYTL 107
G E+ S +++ +Q + + + ++ + R GI+ +QG T Y L
Sbjct 41 GFRYEVQLASRVSNLTAEQMVDKRAELKLYRNSQLVQRVHGIVRAFSQGDIGHHHTFYQL 100
Query 108 NIQDSTALWHKRRNSRVFMNKSVAEISEILFKEWQNRSSLFAASLTLDLSGLAQNYDIRP 167
+ + R NSR+F ++V EI IL +E FA L ++ R
Sbjct 101 TLVPALERLSLRHNSRIFQKQTVPEILSILLQEMGINDYAFA---------LKRDGVQRE 151
Query 168 FTMQINETDYEFLTRLWRSEGINW-LVDE--KNTVVTHPLQPIAPQKLRLIDDNQQFLAL 224
F +Q E+D +FL RL EG+ + V E K+T+ A L + + + AL
Sbjct 152 FCVQYRESDIDFLHRLAAEEGLVYSFVHEAGKHTLYFSD----ASDSLSKLPEPIPYNAL 207
Query 225 SRGLIRFHRSHATEHFDSMTHLVAHRQLQSTAAYVQRWQADGLLQDEGSGSVLSAHQHSD 284
G I T + +T+ T A V Q + + S L Q ++
Sbjct 208 VGGAID------TPYIHGLTY--------RTQAEVSEVQLKDYSFKKPAYSFLQTVQGTE 253
Query 285 QQSNESLSLEQAWSIGSAWTTDLTGADQATASSSQQIEKLNQNLTAYQERQAKYFIAQTT 344
++ Q + + D+ GA +Q Y R A Q+
Sbjct 254 LDYQQTRY--QHFDAPGRYKDDVNGA------------AFSQIRLDYLRRHAHTATGQSN 299
Query 345 VRDAQVGYWFELQGHPEIDQHDLADRQFLIYEKRFFNQNNLPKDIQQQFEYLLMHSALKT 404
+ GY F+LQ H + +R +++ + H +
Sbjct 300 EPLLRAGYKFDLQEHLD----PAMNRDWVVVS--------------------INHQGEQP 335
Query 405 SNQQERQGNELTLIRRQVKVVPEY-----QPLMHRPVVYPQRARVVGPENEEIYVDEWGR 459
QE G+ T Q ++P + +P V P A VVGPE EEI+ DE GR
Sbjct 336 QALQEDGGSGATTYSNQFSLIPGHLHWRAEPQPKPQVDGPMIATVVGPEGEEIFCDEHGR 395
Query 460 IKVRFLFTRTEDHAHDGGAGSNDNDTDSAWVDVLTPWAGEGYGARFLPRVGELVVIDFFD 519
+K+ F + R SN N+ S WV V WAG YG +PR+G V+++F +
Sbjct 396 VKIHFPWDRY----------SNGNEQSSCWVRVSQGWAGSQYGFIAIPRIGHEVIVEFLN 445
Query 520 GHVDRPFATGRIHEAQRSPTKFDIRGQLPATRNLSGIRSQEIAGSGFNQLRFDDTTGQ 577
G D+P TGR + A +P LP + + +R++ G GFN+L F+D G+
Sbjct 446 GDPDQPIITGRTYHATNTPPY-----TLPEHKTKTVLRTETHQGEGFNELSFEDQAGK 498
Score = 26.6 bits (57), Expect = 0.077
Identities = 15/42 (36%), Positives = 21/42 (50%), Gaps = 0/42 (0%)
Query 958 EIDIKAGGSQLIVNGNGVFIKTGGKFEVRSGQHVFTSGEKVS 999
EI +KAG +I G+ + +K GG F V SG V+
Sbjct 564 EIHVKAGQKVVIEAGSEITVKAGGSFVKVDPAGVHLSGALVN 605
Score = 26.6 bits (57), Expect = 0.077
Identities = 19/62 (31%), Positives = 30/62 (48%), Gaps = 2/62 (3%)
Query 945 ISTEDKIELTSTKEIDIKAGGSQLIVNGNGVFIKTGGKFEVRSGQHVFTSGEKVSYEVPE 1004
+ K+ + + EI +KAGGS + V+ GV + +G + SG SG +P
Sbjct 567 VKAGQKVVIEAGSEITVKAGGSFVKVDPAGVHL-SGALVNLNSGGSA-GSGSGFGGAMPA 624
Query 1005 LP 1006
LP
Sbjct 625 LP 626
>E0SIS4 Putative type VI secretion system protein VgrGB [Dickeya
dadantii (strain 3937)]
Length=617
Score = 123 bits (308), Expect = 5e-32
Identities = 149/602 (25%), Positives = 239/602 (40%), Gaps = 108/602 (18%)
Query 85 RTSGIITRATQGQSDGALTLYTLNIQDSTALWHK--RRNSRVFMNKSVAEISEILFKEWQ 142
R SGI++R QG + T Y ++ ALW R N+R+F + I L +E
Sbjct 72 RVSGIVSRFAQGDTGFRRTRYQAEVRP--ALWRLGLRTNARIFQTQKPDAIISTLLEE-- 127
Query 143 NRSSLFAASLTLDLSGLAQNYDIRPFTMQINETDYEFLTRLWRSEGINWLVDEKNTVVTH 202
A +T L ++ +R + +Q E+D F IN L E+ H
Sbjct 128 -------AGITDYAFALRHDHAVREYCVQYRESDLAF---------INRLAAEEGLFYFH 171
Query 203 PLQPIAPQKLRLIDDNQQFLALSRGLIRFHRSHATEHFDSMTHLVAHRQLQSTAA----- 257
+ ++ DD AL++G F AT+ ++ R ++ +
Sbjct 172 EFEA-GKHRVVFADDAG---ALAKGPELFFNL-ATQGLSEGAYVRRFRYAEAVSTAEVAL 226
Query 258 --YVQRWQADGLLQDEGSGSVLSAHQHSDQQSNESLSLEQAWSIGSAWTTDLTGADQATA 315
Y + A GLL ++ S + AHQ Q + + G A+T A +A A
Sbjct 227 KDYSFKTPAYGLLHNKMSSEL--AHQRESYQHFDYPGRFKQDPSGKAFTGYRLDALRAGA 284
Query 316 SSSQQIEKLNQNLTAYQERQAKYFIAQTTVRDAQVGYWFELQGHPEIDQHDLADRQFLIY 375
+ ++ + + G F L HP
Sbjct 285 MTGN---------------------GESNAAELRPGSSFTLTEHPN-------------- 309
Query 376 EKRFFNQNNLPKDIQQQFEYLLMHSALKTSNQQERQGNELTLIRRQVKVVPEYQP----L 431
NL + + HS + +E G E T + +VV L
Sbjct 310 -----PAFNLAWQV-----VAVAHSGQQPQALEEESGGEPTTLSNSFEVVKATTTWRAAL 359
Query 432 MHRPVVY-PQRARVVGPENEEIYVDEWGRIKVRFLFTRTEDHAHDGGAGSNDNDTDSAWV 490
++P+V PQ A VVGP EEIY DE+GR+K++F + R G++D D S WV
Sbjct 360 PYKPMVDGPQIATVVGPAGEEIYCDEFGRVKLQFPWDRY---------GASD-DQSSCWV 409
Query 491 DVLTPWAGEGYGARFLPRVGELVVIDFFDGHVDRPFATGR-IHEAQRSPTKFDIRGQLPA 549
V WAG YG +PR+G VV+ F +G D+P TGR H SP LPA
Sbjct 410 RVSQGWAGGQYGLIAIPRIGHEVVVSFLEGDPDQPIVTGRTFHATNPSPY------PLPA 463
Query 550 TRNLSGIRSQEIAGSGFNQLRFDDTTGQISTQLQSSHGASQLNLGNLSHPKNEETSQGRG 609
++ + +R+ G+GFN+LRF+D GQ + + + + L N S N ++ G
Sbjct 464 SKTRTSLRTSTHKGAGFNELRFEDQAGQEEVFIHAQKDMNTVVLNNRSTSVNASHTENVG 523
Query 610 EGFELRTDHYGAVRAGKGMLISTYAQEQAIADHLEAAQAQSLLAQSHENMKTLSDIALKQ 669
+ H V K ++ EQ +A + + +E+++ +IA++
Sbjct 524 GDQTVVVQHNQTVSV-KENQVTEIQGEQTVA----VTKNRHTTVDDNESLQVKKNIAIQS 578
Query 670 QT 671
Q+
Sbjct 579 QS 580
>E0SAL0 Putative type VI secretion system protein VgrGA [Dickeya
dadantii (strain 3937)]
Length=617
Score = 117 bits (292), Expect = 5e-30
Identities = 86/279 (31%), Positives = 134/279 (48%), Gaps = 27/279 (10%)
Query 399 HSALKTSNQQERQGNELTLIRRQVKVVPEYQP----LMHRPVVY-PQRARVVGPENEEIY 453
HS + +E G E T + +VV + ++P+V PQ A VVGP EEIY
Sbjct 323 HSGQQPQALEEESGGEPTTLSNSFEVVKGTTTWRAAMPYKPMVDGPQIATVVGPAGEEIY 382
Query 454 VDEWGRIKVRFLFTRTEDHAHDGGAGSNDNDTDSAWVDVLTPWAGEGYGARFLPRVGELV 513
DE+GRIK++F + R G++D D S WV V WAG YG +PR+G V
Sbjct 383 CDEFGRIKLQFPWDRY---------GASD-DQSSCWVRVSQGWAGGQYGLIAIPRIGHEV 432
Query 514 VIDFFDGHVDRPFATGR-IHEAQRSPTKFDIRGQLPATRNLSGIRSQEIAGSGFNQLRFD 572
V+ F +G D+P TGR H SP LPA++ + +R+ G+GFN+LRF+
Sbjct 433 VVSFLEGDPDQPIVTGRTFHATNPSPY------PLPASKTRTSLRTSTHKGAGFNELRFE 486
Query 573 DTTGQISTQLQSSHGASQLNLGNLSHPKNEETSQGRGEGFELRTDHYGAVRAGKGMLIST 632
D GQ + + + + L N S N ++ G + H V K ++
Sbjct 487 DQAGQEEVFIHAQKDMNTVVLNNRSTSVNASHTENVGGDQTVVVQHNQTVSV-KENQVTE 545
Query 633 YAQEQAIADHLEAAQAQSLLAQSHENMKTLSDIALKQQT 671
EQ +A Q ++ +E+++ ++IA++ Q+
Sbjct 546 IQGEQTVA----VTQNRNTTVNDNESLQVKNNIAIQSQS 580
Score = 42.0 bits (97), Expect = 1e-06
Identities = 31/107 (29%), Positives = 48/107 (45%), Gaps = 13/107 (12%)
Query 85 RTSGIITRATQGQSDGALTLYTLNIQDSTALWH--KRRNSRVFMNKSVAEISEILFKEWQ 142
R SGI++R QG + T Y ++ ALW R N+R+F + I L +E
Sbjct 72 RVSGIVSRFAQGDTGFRRTRYQAEVR--PALWRLGLRTNARIFQTQKPDAIIGTLLEE-- 127
Query 143 NRSSLFAASLTLDLSGLAQNYDIRPFTMQINETDYEFLTRLWRSEGI 189
A +T L + +R + +Q E+D F+ RL EG+
Sbjct 128 -------AGITDFAFALRHEHAVREYCVQYRESDLAFINRLAAEEGL 167
>Q9I0F3 Type VI secretion system spike protein VgrG1c [Pseudomonas
aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116
/ JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)]
Length=726
Score = 102 bits (255), Expect = 2e-25
Identities = 68/212 (32%), Positives = 109/212 (51%), Gaps = 35/212 (17%)
Query 380 FNQNNLPKDIQQQFEYLLMHSALKTSNQQERQGN---------ELTLIRRQVKVVPEYQP 430
F+ +N P+ + Q EYL++ + + + GN EL I ++P
Sbjct 341 FHLSNYPR-LDQNREYLVVGAEYRVVQELYETGNGGGGAQFESELDCI----DAGQAFRP 395
Query 431 LMHRPVVY---PQRARVVGPENEEIYVDEWGRIKVRFLFTRTEDHAHDGGAGSNDNDTDS 487
L PV PQ A VVGP+ EEI+ D++GR+KV F + R HD N+ S
Sbjct 396 LPSTPVPVVRGPQTAVVVGPKGEEIWTDQYGRVKVHFHWDR-----HD-----QSNENSS 445
Query 488 AWVDVLTPWAGEGYGARFLPRVGELVVIDFFDGHVDRPFATGRIHEAQRSPTKFDIRGQL 547
W+ V WAG+ +G+ +PR+G+ V++ F +G DRP TGR++ A+++ + +L
Sbjct 446 CWMRVSQAWAGKNWGSIQIPRIGQEVIVSFLEGDPDRPIITGRVYNAEQT-----VPYEL 500
Query 548 PATRNLSGIRSQEIAG---SGFNQLRFDDTTG 576
PA SG +S+ G + FN++R +D G
Sbjct 501 PANATQSGTKSRSSKGGTPANFNEIRMEDKKG 532
Score = 59.7 bits (143), Expect = 5e-12
Identities = 46/184 (25%), Positives = 82/184 (45%), Gaps = 12/184 (7%)
Query 14 FGALKRAIHVQFSNPLLNTQIFLQRIDGKHTLNEGLEVELTCLSINAHIALKQFIGCQVA 73
F R +HV +PL + LQR++G+ L EL +S N + L +G ++
Sbjct 40 FSQHTRLVHVD--SPLGPEVLQLQRLEGREELGRLFSHELELVSSNPALPLDALLGKPMS 97
Query 74 IDQVTDTGQLARTSGIITRATQGQSDGALTLYTLNIQDSTALWHKRRNSRVFMNKSVAEI 133
+ G GI+ R +QG G Y + ++ L + + R+F N+ V +I
Sbjct 98 LALELPGGSRRYFHGIVARCSQGAGAGQFASYQVTLRPWLWLLTRTSDCRIFQNQKVPDI 157
Query 134 SEILFKEWQNRSSLFAASLTLDLSGLAQNYDIRPFTMQINETDYEFLTRLWRSEGI-NWL 192
+ +F++ + L+++Y + +Q ET ++F++RL EGI W
Sbjct 158 IKQVFRD---------LGFSDFEDALSRSYREWEYCVQYRETSFDFVSRLMEQEGIYYWF 208
Query 193 VDEK 196
EK
Sbjct 209 RHEK 212
Score = 21.9 bits (45), Expect = 1.9
Identities = 12/45 (27%), Positives = 21/45 (47%), Gaps = 4/45 (9%)
Query 507 PRVGELVVIDFFDGHVDRPFATGRI----HEAQRSPTKFDIRGQL 547
P +G FFD H+ R G + ++ QR T+ ++R +
Sbjct 240 PSLGHRERDHFFDWHMAREVQPGSLSLNDYDFQRPGTRLEVRSNV 284
Score = 20.0 bits (40), Expect = 7.5
Identities = 8/19 (42%), Positives = 14/19 (74%), Gaps = 0/19 (0%)
Query 950 KIELTSTKEIDIKAGGSQL 968
+IELT+ +EI ++ G S +
Sbjct 690 RIELTADREILLRCGQSTI 708
>Q9I737 Type VI secretion system spike protein VgrG1b [Pseudomonas
aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116
/ JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)]
Length=741
Score = 102 bits (255), Expect = 2e-25
Identities = 56/155 (36%), Positives = 89/155 (57%), Gaps = 21/155 (14%)
Query 428 YQPLMHRPVVY---PQRARVVGPENEEIYVDEWGRIKVRFLFTRTEDHAHDGGAGSNDND 484
Y+PL P+ PQ A VVGP+ EEI+ D++GR+KV F + R HD N+
Sbjct 356 YRPLPTTPLPIVRGPQTAVVVGPKGEEIWTDQYGRVKVHFHWDR-----HD-----QSNE 405
Query 485 TDSAWVDVLTPWAGEGYGARFLPRVGELVVIDFFDGHVDRPFATGRIHEAQRSPTKFDIR 544
S W+ V WAG+ +G+ +PR+G+ V++ F +G DRP TGR++ A+++ +
Sbjct 406 NSSCWIRVSQAWAGKNWGSMQIPRIGQEVIVSFLEGDPDRPIITGRVYNAEQT-----VP 460
Query 545 GQLPATRNLSGIRSQEIAG---SGFNQLRFDDTTG 576
+LPA SG++S+ G + FN++R +D G
Sbjct 461 YELPANATQSGMKSRSSKGGTPANFNEIRMEDKKG 495
Score = 58.2 bits (139), Expect = 1e-11
Identities = 39/167 (23%), Positives = 75/167 (45%), Gaps = 9/167 (5%)
Query 23 VQFSNPLLNTQIFLQRIDGKHTLNEGLEVELTCLSINAHIALKQFIGCQVAIDQVTDTGQ 82
V+ +PL + LQR++G+ L EL +S N + L +G ++ G
Sbjct 10 VRVDSPLGAEVLQLQRMEGREELGRPFAYELELISENPDLPLDGLLGKPASLALELHDGS 69
Query 83 LARTSGIITRATQGQSDGALTLYTLNIQDSTALWHKRRNSRVFMNKSVAEISEILFKEWQ 142
GI+ +QG +G Y + ++ L + + R+F N+ V +I + +F++
Sbjct 70 RRHFHGIVAACSQGSGNGQFASYQVTLRPWLWLLTRTSDCRIFQNQKVPDIIKQVFRD-- 127
Query 143 NRSSLFAASLTLDLSGLAQNYDIRPFTMQINETDYEFLTRLWRSEGI 189
+ L+++Y + +Q ET ++F++RL EGI
Sbjct 128 -------LGFSDFEDALSRSYREWEYCVQYRETSFDFVSRLMEQEGI 167
Score = 23.5 bits (49), Expect = 0.65
Identities = 9/25 (36%), Positives = 17/25 (68%), Gaps = 0/25 (0%)
Query 876 ILQASQDIAESASGSINLSAQKNII 900
I+ + ++I SG+IN+ A KN++
Sbjct 708 IVISGKNITIDGSGAINVKADKNVV 732
Lambda K H a alpha
0.316 0.132 0.376 0.792 4.96
Gapped
Lambda K H a alpha sigma
0.267 0.0410 0.140 1.90 42.6 43.6
Effective search space used: 7759317
Database: 0bc3461723e75f4e0db7b86576c541ef.SwissProt.fasta
Posted date: May 13, 2024 8:56 PM
Number of letters in database: 8,011
Number of sequences in database: 10
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 11
Window for multiple hits: 40
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Data ...
