BLASTP 2.12.0+
Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics: Alejandro A. Schaffer,
L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri
I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids
Res. 29:2994-3005.
Database: 5d2df089b73f2679daadfde192f791ac.SwissProt.fasta
10 sequences; 2,431 total letters
Query= ACIAD0318
Length=237
Score E
Sequences producing significant alignments: (Bits) Value
O93779 Aspartate racemase [Thermococcus sp. (strain KS-8)] 134 1e-42
O58403 Aspartate racemase [Pyrococcus horikoshii (strain ATCC 700... 134 1e-42
P32968 Uncharacterized protein in pnlA 3'region [Pectobacterium c... 109 6e-34
P32960 Broad specificity amino-acid racemase RacX [Bacillus subti... 80.1 7e-22
B4UA20 Glutamate racemase [Hydrogenobaculum sp. (strain Y04AAS1)] 47.0 5e-10
Q9PM24 Glutamate racemase [Campylobacter jejuni subsp. jejuni ser... 46.6 7e-10
A8FFY8 Glutamate racemase [Bacillus pumilus (strain SAFR-032)] 38.5 4e-07
Q02YQ3 Glutamate racemase [Lactococcus lactis subsp. cremoris (st... 37.0 1e-06
A2RKH0 Glutamate racemase [Lactococcus lactis subsp. cremoris (st... 36.6 2e-06
Q9CG28 Glutamate racemase [Lactococcus lactis subsp. lactis (stra... 33.9 1e-05
>O93779 Aspartate racemase [Thermococcus sp. (strain KS-8)]
Length=232
Score = 134 bits (338), Expect = 1e-42
Identities = 75/224 (33%), Positives = 123/224 (55%), Gaps = 4/224 (2%)
Query 3 KAIGILGGMGPQATVDAMNKIIKNTSAHSDQEHIPVIIVSIPDIPDRTKSIINHDDKPFE 62
+ IGILGGMGP AT D +I++ T A DQ+H +II + P IPDRT I+ + P
Sbjct 4 RVIGILGGMGPLATADLFRRIVEKTPAKRDQDHPRIIIYNNPKIPDRTAFILGNGPDPRP 63
Query 63 KMREYLKILENANVGCIIIPCNTAHFWFDQLQQQTHTKMISIIEATVRFIQKSNAQEICI 122
++ + LE II+PCNTAHF+ +Q ++S+IE T + I++ +++ +
Sbjct 64 ELITSARKLEECGADFIIMPCNTAHFFAGTIQSSVIYPLVSMIEETAKRIEEMGLRKVGL 123
Query 123 LATSATINTELYQKELANHQIKFTLPELEIQQLVMQSIY-SYKSGNIEEAKNIMINIINL 181
LAT TI +Y + L I +P + Q LVM++IY K+GN+E + +++ +
Sbjct 124 LATDGTIKGMVYHRALLKRGIHIAVPNKKDQGLVMKAIYEGVKAGNLELGRELLLKVAKK 183
Query 182 YEYNPARKFLLACTEIPLILEDWIIEKPELFIDATDVLIRNAIE 225
E + + CTE+ ++L+ + P ID DV+ A++
Sbjct 184 LE-RRSEGIIAGCTEVSVVLKPGDLTVP--LIDPMDVIAERAVK 224
>O58403 Aspartate racemase [Pyrococcus horikoshii (strain ATCC
700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)]
Length=228
Score = 134 bits (337), Expect = 1e-42
Identities = 75/224 (33%), Positives = 127/224 (57%), Gaps = 3/224 (1%)
Query 3 KAIGILGGMGPQATVDAMNKIIKNTSAHSDQEHIPVIIVSIPDIPDRTKSIINHDDKPFE 62
K IGILGGMGP AT + +I+ T A DQEH VII + P IPDRT I+ + P
Sbjct 2 KTIGILGGMGPLATAELFRRIVIKTPAKRDQEHPKVIIFNNPQIPDRTAYILGKGEDPRP 61
Query 63 KMREYLKILENANVGCIIIPCNTAHFWFDQLQQQTHTKMISIIEATVRFIQKSNAQEICI 122
++ K LE II+PCNTAH + + +++ +IS+IE T + +++ ++ +
Sbjct 62 QLIWTAKRLEECGADFIIMPCNTAHAFVEDIRKAIKIPIISMIEETAKKVKELGFKKAGL 121
Query 123 LATSATINTELYQKELANHQIKFTLPELEIQQLVMQSIY-SYKSGNIEEAKNIMINIINL 181
LAT+ TI + +Y+KE + + ++ P + Q+ VM+ IY K+GN++ + +++ +
Sbjct 122 LATTGTIVSGVYEKEFSKYGVEIMTPTEDEQKDVMRGIYEGVKAGNLKLGRELLLKTAKI 181
Query 182 YEYNPARKFLLACTEIPLILEDWIIEKPELFIDATDVLIRNAIE 225
E A + CTE+ ++L+ ++ P ID DV+ A++
Sbjct 182 LEERGAECIIAGCTEVSVVLKQDDLKVP--LIDPMDVIAEVAVK 223
>P32968 Uncharacterized protein in pnlA 3'region [Pectobacterium
carotovorum subsp. carotovorum]
Length=150
Score = 109 bits (273), Expect = 6e-34
Identities = 54/139 (39%), Positives = 84/139 (60%), Gaps = 0/139 (0%)
Query 89 WFDQLQQQTHTKMISIIEATVRFIQKSNAQEICILATSATINTELYQKELANHQIKFTLP 148
WF+ L+QQ +MISII+ T + I+ +N + +LAT+AT+ +YQ L I P
Sbjct 1 WFNDLKQQCRAEMISIIDVTCQAIKHANTTRVGLLATTATVKARIYQDNLITDNIDCYTP 60
Query 149 ELEIQQLVMQSIYSYKSGNIEEAKNIMINIINLYEYNPARKFLLACTEIPLILEDWIIEK 208
+ Q VM+SIY+YKSG+I A +++ + + K +L CTE+PLILE +
Sbjct 61 DDADQHQVMESIYAYKSGDIAGAYSLLSPVKDRLLQAGVEKIILGCTELPLILEQEVRTS 120
Query 209 PELFIDATDVLIRNAIEWY 227
P+ ++DAT+ LI+ +EWY
Sbjct 121 PQHYVDATEELIKKTVEWY 139
Score = 19.2 bits (38), Expect = 0.54
Identities = 8/15 (53%), Positives = 10/15 (67%), Gaps = 0/15 (0%)
Query 13 PQATVDAMNKIIKNT 27
PQ VDA ++IK T
Sbjct 121 PQHYVDATEELIKKT 135
>P32960 Broad specificity amino-acid racemase RacX [Bacillus subtilis
(strain 168)]
Length=227
Score = 80.1 bits (196), Expect = 7e-22
Identities = 66/227 (29%), Positives = 113/227 (50%), Gaps = 10/227 (4%)
Query 5 IGILGGMGPQATVDAMNKII----KNTSAHSDQEHIPVIIVSIPDIPDRTKSIINHDDKP 60
IGIL GMGP++T ++K+I K A +D ++ ++I S P P I+HD+
Sbjct 2 IGILAGMGPKSTSPFIDKVIDYCQKLYGASNDIDYPHMMIYSCPT-PFYADRPIDHDEMK 60
Query 61 FEKMREYLKILENANVGCIIIPCNTAHFWFDQLQQQTHTKMISIIEATVRFIQKSNAQEI 120
+ +K LE V I +PCNTAH +++++QQ M+ I+E T++ I A++
Sbjct 61 KAIIDGAVK-LEKTGVDFIALPCNTAHVYYEEIQQALSVPMLHIVEETIKEIPHP-AKKA 118
Query 121 CILATSATINTELYQKELANHQIKFTLPELEIQQLVMQSIYSYKSGNIEEAKNIMINIIN 180
+L T TI + +YQK L + + + + QQ V Q I + K N + + +
Sbjct 119 VVLGTEPTIQSAIYQKVLKGNG-QEVIHKDHWQQAVNQLIAAIKQPNHMQHTQALWQTLY 177
Query 181 LYEYNPARKFLLACTEIPLILEDWIIEKPELFIDATDVLIRNAIEWY 227
A + ACT++ +L+ E P ID++ L ++ + Y
Sbjct 178 EEISQHADIIISACTDLNAVLDHIQSEIP--IIDSSACLAKSTVSTY 222
>B4UA20 Glutamate racemase [Hydrogenobaculum sp. (strain Y04AAS1)]
Length=259
Score = 47.0 bits (110), Expect = 5e-10
Identities = 43/160 (27%), Positives = 70/160 (44%), Gaps = 8/160 (5%)
Query 65 REYLKILENANVGCIIIPCNTAHF-WFDQLQQQTHTKMISIIEATVRFIQKSN-AQEICI 122
+E L N NV I+I CN+ + LQ+ ++ +IEA V KS+ + + I
Sbjct 52 KESANFLINQNVDVILIACNSVSANAMEALQEMFDIPIVGVIEAGVEAALKSSKTKHVGI 111
Query 123 LATSATINTELYQKELANHQIKFTLPELEIQQLVMQSIYSYKSGNIEEAKN-IMINIINL 181
+ TSATIN+ YQ+ L IK + I K N + +K + +
Sbjct 112 IGTSATINSNKYQERLEAFNIKTYAKACPL----FVPIVEEKLINTDISKKAVEFYLKEF 167
Query 182 YEYNPARKFLLACTEIPLILEDWIIEKPEL-FIDATDVLI 220
+ +L CT PL+ ED P + + ++D +I
Sbjct 168 KDLKDIDTLILGCTHYPLLEEDIKAFLPHINLVSSSDAII 207
>Q9PM24 Glutamate racemase [Campylobacter jejuni subsp. jejuni
serotype O:2 (strain ATCC 700819 / NCTC 11168)]
Length=250
Score = 46.6 bits (109), Expect = 7e-10
Identities = 29/78 (37%), Positives = 43/78 (55%), Gaps = 6/78 (8%)
Query 66 EYLKILENANVGCIIIPCNTAHFW-FDQLQQQTHTKMISII----EATVRFIQKSNAQEI 120
E L E + +II CNTA + D L+ + H + +I EAT++ + N +EI
Sbjct 53 EALDFFEQFQIDMLIIACNTASAYALDALRAKAHFPVYGVIDAGVEATIKALHDKN-KEI 111
Query 121 CILATSATINTELYQKEL 138
++AT ATI +E YQK L
Sbjct 112 LVIATKATIKSEEYQKRL 129
Score = 16.2 bits (30), Expect = 6.1
Identities = 5/12 (42%), Positives = 8/12 (67%), Gaps = 0/12 (0%)
Query 84 NTAHFWFDQLQQ 95
NTA W + L++
Sbjct 239 NTAKIWLNLLRK 250
>A8FFY8 Glutamate racemase [Bacillus pumilus (strain SAFR-032)]
Length=272
Score = 38.5 bits (88), Expect = 4e-07
Identities = 34/144 (24%), Positives = 68/144 (47%), Gaps = 10/144 (7%)
Query 63 KMREYLKILENANVGCIIIPCNTAH-FWFDQLQQQTHTKMISIIEATVRF-IQKSNAQEI 120
+M YL L + ++ ++I CNTA D+++ +I +I+ R I+ +N Q+I
Sbjct 56 EMAHYL--LRHHHIKMLVIACNTATAIALDEIKATLDIPVIGVIQPGARTAIKVTNNQQI 113
Query 121 CILATSATINTELYQKELANHQIKFTLPELEIQQLVMQSIYSYKSGNI--EEAKNIMINI 178
++ T+ TI +E Y++ L + + + L LV +SG + A ++ +
Sbjct 114 GVIGTANTIKSEAYKEALLSLKAGLAVQSLACPLLVP----FVESGTFLDQTADEVVKDS 169
Query 179 INLYEYNPARKFLLACTEIPLILE 202
+ + +L CT P++ E
Sbjct 170 LEPMKETGIDTLILGCTHYPILKE 193
Score = 16.5 bits (31), Expect = 4.6
Identities = 13/37 (35%), Positives = 16/37 (43%), Gaps = 3/37 (8%)
Query 35 HIPVIIVSIPDIPDRTKSIINHDDKPFEKMREYLKIL 71
H P++ SI SII+ D E RE IL
Sbjct 187 HYPILKESIQRFMGSDVSIISSGD---ETAREASTIL 220
>Q02YQ3 Glutamate racemase [Lactococcus lactis subsp. cremoris
(strain SK11)]
Length=271
Score = 37.0 bits (84), Expect = 1e-06
Identities = 33/137 (24%), Positives = 60/137 (44%), Gaps = 5/137 (4%)
Query 66 EYLKILENANVGCIIIPCNTAH-FWFDQLQQQTHTKMISII-EATVRFIQKSNAQEICIL 123
+ + L + NV I++ CNTA + ++++ +I +I IQK+ +I ++
Sbjct 56 DMVNFLLSKNVKMIVMACNTATAMTLEIVKEELDIPVIGVILPGASSAIQKTKTNKIGVI 115
Query 124 ATSATINTELYQKELANHQIKFTLPELEIQQLVMQSIYSYKSGNIEEAKNIMINIINLYE 183
AT A+I ++ Y K +A + L + V SI E AK ++ +
Sbjct 116 ATQASIRSDEYHKTIARKSSAIEVLSLACPKFV--SIVESNEMESEIAKRVVSESLAPL- 172
Query 184 YNPARKFLLACTEIPLI 200
+L CT PL+
Sbjct 173 VGKIDTLILGCTHYPLL 189
>A2RKH0 Glutamate racemase [Lactococcus lactis subsp. cremoris
(strain MG1363)]
Length=271
Score = 36.6 bits (83), Expect = 2e-06
Identities = 33/137 (24%), Positives = 60/137 (44%), Gaps = 5/137 (4%)
Query 66 EYLKILENANVGCIIIPCNTAHFW-FDQLQQQTHTKMISII-EATVRFIQKSNAQEICIL 123
+ + L + NV I++ CNTA + ++++ +I +I IQK+ +I ++
Sbjct 56 DMVNFLLSKNVKMIVMACNTATAMALEIVKEELDIPVIGVILPGASSAIQKTKTNKIGVI 115
Query 124 ATSATINTELYQKELANHQIKFTLPELEIQQLVMQSIYSYKSGNIEEAKNIMINIINLYE 183
AT A+I ++ Y K +A + L + V SI E AK ++ +
Sbjct 116 ATQASIRSDEYHKTIARKSSAIEVLSLACPKFV--SIVESNEMESEIAKRVVSESLAPL- 172
Query 184 YNPARKFLLACTEIPLI 200
+L CT PL+
Sbjct 173 VGKIDTLILGCTHYPLL 189
>Q9CG28 Glutamate racemase [Lactococcus lactis subsp. lactis (strain
IL1403)]
Length=271
Score = 33.9 bits (76), Expect = 1e-05
Identities = 32/141 (23%), Positives = 62/141 (44%), Gaps = 13/141 (9%)
Query 66 EYLKILENANVGCIIIPCNTAHFW-FDQLQQQTHTKMISII-EATVRFIQKSNAQEICIL 123
+ + L + +V I++ CNTA + ++++ +I +I IQK+ +I ++
Sbjct 56 DMVNFLLSKDVKMIVMACNTATAMALEIVKEKLDIPVIGVILPGASSAIQKTKTNKIGVI 115
Query 124 ATSATINTELYQKELANHQIKFTLPELEIQQLVMQSIYSYKSGNIEEA----KNIMINII 179
AT A+I ++ Y K +A +E+ L S N E+ K + +++
Sbjct 116 ATQASIRSDEYHKTIARKS-----SAVEVYSLACPKFVSIVESNEMESEIARKVVSESLV 170
Query 180 NLYEYNPARKFLLACTEIPLI 200
L +L CT PL+
Sbjct 171 PL--IGKVDTLILGCTHYPLL 189
Lambda K H a alpha
0.322 0.137 0.406 0.792 4.96
Gapped
Lambda K H a alpha sigma
0.267 0.0410 0.140 1.90 42.6 43.6
Effective search space used: 404118
Database: 5d2df089b73f2679daadfde192f791ac.SwissProt.fasta
Posted date: May 31, 2024 6:51 PM
Number of letters in database: 2,431
Number of sequences in database: 10
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 11
Window for multiple hits: 40
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Data ...
