BLASTP 2.12.0+
Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics: Alejandro A. Schaffer,
L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri
I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids
Res. 29:2994-3005.
Database: aadeec86424a8bdfdfbc5d02f7bb10ab.SwissProt.fasta
5 sequences; 1,378 total letters
Query= ACIAD0345
Length=203
Score E
Sequences producing significant alignments: (Bits) Value
Q32L00 Alpha-ketoglutarate-dependent dioxygenase alkB homolog 3 [... 119 1e-36
Q6P6J4 DNA oxidative demethylase ALKBH2 [Mus musculus] 115 7e-36
Q58DM4 DNA oxidative demethylase ALKBH2 [Bos taurus] 115 1e-35
Q6NS38 DNA oxidative demethylase ALKBH2 [Homo sapiens] 112 2e-34
Q9SIE0 DNA oxidative demethylase ALKBH2 [Arabidopsis thaliana] 94.4 3e-27
>Q32L00 Alpha-ketoglutarate-dependent dioxygenase alkB homolog
3 [Bos taurus]
Length=286
Score = 119 bits (297), Expect = 1e-36
Identities = 69/182 (38%), Positives = 91/182 (50%), Gaps = 12/182 (7%)
Query 27 FISTAEAEQYFQYFLKHLAWRHDEARLYGKHFITPRQVAWYGDDHYRYAYSGIMRDSLP- 85
F+ EA+ F+ + + W+ + PR AWYG+ Y YS I + P
Sbjct 96 FVDLKEADSVFEQLCRDVPWKQRTGIRDDVTYQQPRLTAWYGE--LPYTYSRITMEPNPH 153
Query 86 WDPQLLILKQQIEQTLAERFNSCLANLYQEGTQGMAWHSDGDMSLAKQTTIASLSLGATR 145
W P LL+LK QIE+ FNS L NLY+ + WHSD + SL + IASLS GATR
Sbjct 154 WHPVLLMLKNQIEENTGHSFNSLLCNLYRNEKDSVDWHSDDEPSLGRCPIIASLSFGATR 213
Query 146 KFCFR---------HLRTKEKIEMQLQSGQLIVMRGETQQHWQHAIMKSQKVIHPRINLT 196
F R E++++ L G L++M G TQ WQH + K PRINLT
Sbjct 214 MFEMRKKPPPEDNGDYTYVERVKIPLDHGTLLIMEGATQADWQHRVPKEYHSREPRINLT 273
Query 197 FR 198
FR
Sbjct 274 FR 275
>Q6P6J4 DNA oxidative demethylase ALKBH2 [Mus musculus]
Length=239
Score = 115 bits (288), Expect = 7e-36
Identities = 67/194 (35%), Positives = 100/194 (52%), Gaps = 19/194 (10%)
Query 23 DYGCFISTAEAEQYF-------QYFLKHLAWRHDEARLYGKHFITPRQVAWYGDDHYRYA 75
DY AEA++ F +YF LA + +++GK PR+ A YGD Y
Sbjct 46 DYTVLFGKAEADKIFRELEQEVEYFTGALA----KVQVFGKWHSVPRKQATYGDAGLTYT 101
Query 76 YSGIMRDSLPWDPQLLILKQQIEQTLAERFNSCLANLYQEGTQGMAWHSDGDMSLAKQTT 135
+SG+ PW P L ++ ++ + + FN L N Y++G + H D + LA +
Sbjct 102 FSGLTLTPKPWVPVLERVRDRVCEVTGQTFNFVLVNRYKDGCDHIGEHRDDERELAPGSP 161
Query 136 IASLSLGATRKFCFRH--------LRTKEKIEMQLQSGQLIVMRGETQQHWQHAIMKSQK 187
IAS+S GA R F FRH RT E + +QL G L++M T HW H++ ++
Sbjct 162 IASVSFGACRDFIFRHKDSRGKRPRRTVEVVRLQLAHGSLLMMNPPTNTHWYHSLPIRKR 221
Query 188 VIHPRINLTFRQFL 201
V+ PR+NLTFR+ L
Sbjct 222 VLAPRVNLTFRKIL 235
>Q58DM4 DNA oxidative demethylase ALKBH2 [Bos taurus]
Length=278
Score = 115 bits (289), Expect = 1e-35
Identities = 68/194 (35%), Positives = 97/194 (50%), Gaps = 19/194 (10%)
Query 23 DYGCFISTAEAEQYFQ-------YFLKHLAWRHDEARLYGKHFITPRQVAWYGDDHYRYA 75
DY AEA++ FQ YF LA +++GK PR+ A YGD Y
Sbjct 67 DYTILFGKAEADEIFQELEKEVEYFTGALA----RVQVFGKWHSVPRKQATYGDTGLTYT 122
Query 76 YSGIMRDSLPWDPQLLILKQQIEQTLAERFNSCLANLYQEGTQGMAWHSDGDMSLAKQTT 135
+SG+ PW P L ++ ++ + FN L N Y++G + H D + LA +
Sbjct 123 FSGLTLSPKPWIPVLERVRDRVSLVTGQTFNFVLINRYKDGQDHIGEHRDDERELALGSP 182
Query 136 IASLSLGATRKFCFRH--------LRTKEKIEMQLQSGQLIVMRGETQQHWQHAIMKSQK 187
IAS+S GA R F FRH R E + +QL G L++M T HW H++ +K
Sbjct 183 IASVSFGACRDFVFRHKDSRGKHPSRRLEVVRLQLAHGSLLMMNHPTNTHWYHSLPVRKK 242
Query 188 VIHPRINLTFRQFL 201
V+ PR+NLTFR+ L
Sbjct 243 VLAPRVNLTFRKIL 256
>Q6NS38 DNA oxidative demethylase ALKBH2 [Homo sapiens]
Length=261
Score = 112 bits (280), Expect = 2e-34
Identities = 66/194 (34%), Positives = 96/194 (49%), Gaps = 19/194 (10%)
Query 24 YGCFISTAEAEQYFQ-------YFLKHLAWRHDEARLYGKHFITPRQVAWYGDDHYRYAY 76
Y AEA++ FQ YF LA +++GK PR+ A YGD Y +
Sbjct 69 YTVLFGKAEADEIFQELEKEVEYFTGALA----RVQVFGKWHSVPRKQATYGDAGLTYTF 124
Query 77 SGIMRDSLPWDPQLLILKQQIEQTLAERFNSCLANLYQEGTQGMAWHSDGDMSLAKQTTI 136
SG+ PW P L ++ + + FN L N Y++G + H D + LA + I
Sbjct 125 SGLTLSPKPWIPVLERIRDHVSGVTGQTFNFVLINRYKDGCDHIGEHRDDERELAPGSPI 184
Query 137 ASLSLGATRKFCFRHLRTKEK--------IEMQLQSGQLIVMRGETQQHWQHAIMKSQKV 188
AS+S GA R F FRH ++ K + + L G L++M T HW H++ +KV
Sbjct 185 ASVSFGACRDFVFRHKDSRGKSPSRRVAVVRLPLAHGSLLMMNHPTNTHWYHSLPVRKKV 244
Query 189 IHPRINLTFRQFLL 202
+ PR+NLTFR+ LL
Sbjct 245 LAPRVNLTFRKILL 258
>Q9SIE0 DNA oxidative demethylase ALKBH2 [Arabidopsis thaliana]
Length=314
Score = 94.4 bits (233), Expect = 3e-27
Identities = 66/222 (30%), Positives = 96/222 (43%), Gaps = 27/222 (12%)
Query 7 APQPKTNLLPYDGDVEDYGCFISTAEAEQYFQYFLKHLAWRHDEARLYGKHFITPRQVAW 66
AP KT L + D+ F+ ++ +F Y KH+ W R++G+ + PR +
Sbjct 93 APSRKTIDLGHGSDLIYIQRFLPFQQSWTFFDYLDKHIPWTRPTIRVFGRSCLQPRDTCY 152
Query 67 YGDDHYR-YAYSGIMRDSLPWD--PQLLILKQQIEQTL-AERFNSCLANLYQEGTQGMAW 122
YSG S WD P L + I + L RFNS L N Y+ + +AW
Sbjct 153 VASSGLTALVYSGYRPTSYSWDDFPPLKEILDAIYKVLPGSRFNSLLLNRYKGASDYVAW 212
Query 123 HSDGDMSLAKQTTIASLSLGATRKFCFRHLRTKEKIE----------------------- 159
H+D + IAS+S G R F + + +E +
Sbjct 213 HADDEKIYGPTPEIASVSFGCERDFVLKKKKDEESSQGKTGDSGPAKKRLKRSSREDQQS 272
Query 160 MQLQSGQLIVMRGETQQHWQHAIMKSQKVIHPRINLTFRQFL 201
+ L+ G L+VMRG TQ+ W H++ K K RINLTFR L
Sbjct 273 LTLKHGSLLVMRGYTQRDWIHSVPKRAKAEGTRINLTFRLVL 314
Lambda K H a alpha
0.327 0.138 0.440 0.792 4.96
Gapped
Lambda K H a alpha sigma
0.267 0.0410 0.140 1.90 42.6 43.6
Effective search space used: 204152
Database: aadeec86424a8bdfdfbc5d02f7bb10ab.SwissProt.fasta
Posted date: May 13, 2024 9:41 AM
Number of letters in database: 1,378
Number of sequences in database: 5
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 11
Window for multiple hits: 40
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Data ...
