BLASTP 2.12.0+
Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics: Alejandro A. Schaffer,
L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri
I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids
Res. 29:2994-3005.
Database: 47a4e1224d602a0b9f95b476187b97cd.SwissProt.fasta
17 sequences; 18,643 total letters
Query= ACIAD0421
Length=402
Score E
Sequences producing significant alignments: (Bits) Value
Q795R8 Uncharacterized protein YtfP [Bacillus subtilis (strain 168)] 117 7e-32
Q18164 Dihydropyrimidine dehydrogenase [NADP(+)] [Caenorhabditis ... 45.8 6e-08
A8XKG6 Probable dihydropyrimidine dehydrogenase [NADP(+)] [Caenor... 45.4 8e-08
Q8U195 Sulfide dehydrogenase subunit alpha [Pyrococcus furiosus (... 43.9 2e-07
P37127 Putative oxidoreductase AegA [Escherichia coli (strain K12)] 43.9 2e-07
Q74FU5 NADPH-Fe(3+) oxidoreductase subunit beta [Geobacter sulfur... 43.1 4e-07
D7AF64 NADPH-Fe(3+) oxidoreductase subunit beta [Geobacter sulfur... 43.1 4e-07
Q8ZNL8 NAD-dependent dihydropyrimidine dehydrogenase subunit PreT... 42.7 4e-07
Q8Z5A6 NAD-dependent dihydropyrimidine dehydrogenase subunit PreT... 42.7 4e-07
Q0JKD0 Glutamate synthase 1 [NADH], chloroplastic [Oryza sativa s... 43.1 4e-07
Q9LV03 Glutamate synthase 1 [NADH], chloroplastic [Arabidopsis th... 43.1 4e-07
Q12680 Glutamate synthase [NADH] [Saccharomyces cerevisiae (strai... 42.7 6e-07
Q0DG35 Glutamate synthase 2 [NADH], chloroplastic [Oryza sativa s... 42.7 6e-07
Q28943 Dihydropyrimidine dehydrogenase [NADP(+)] [Sus scrofa] 42.4 7e-07
Q28007 Dihydropyrimidine dehydrogenase [NADP(+)] [Bos taurus] 42.4 7e-07
O89000 Dihydropyrimidine dehydrogenase [NADP(+)] [Rattus norvegicus] 41.6 1e-06
Q12882 Dihydropyrimidine dehydrogenase [NADP(+)] [Homo sapiens] 41.6 1e-06
>Q795R8 Uncharacterized protein YtfP [Bacillus subtilis (strain
168)]
Length=420
Score = 117 bits (292), Expect = 7e-32
Identities = 98/410 (24%), Positives = 183/410 (45%), Gaps = 22/410 (5%)
Query 1 MPNKQVAIIGAGPAGLACAEMLSELGHYDITVYEQKPSAARKFLMAGKTGLNISHAEDLE 60
M V +IG GP+GL A E G + + ++ RK ++G N+++ +E
Sbjct 1 MKQYDVIVIGGGPSGLMAAIAAGEQGA-GVLLIDKGNKLGRKLAISGGGRCNVTNRLPVE 59
Query 61 IFIARYD-HADWLRPMIQQFSALQIQAWMHGLGIESYIGSSGRIFPNEMKAAPLLRAWLK 119
I + +L +F+ I + LGI+ GR+FP KA ++ A L
Sbjct 60 EIIKHIPGNGRFLYSAFSEFNNEDIIKFFENLGIQLKEEDHGRMFPVTDKAQSVVDALLN 119
Query 120 RLQDRGVKFRYRHQVVNVKDDQLTVLDMVQQQGHTLKVDAIVLACGALSWPQLGSDGKWQ 179
RL+ V R ++ +V + +V G + A+++A G S P GS G
Sbjct 120 RLKQLRVTIRTNEKIKSVLYEDGQAAGIVTNNGEMIHSQAVIIAVGGKSVPHTGSTGDGY 179
Query 180 SWLDAS--VMQPFQPSNVGVLVNWSDYLQ-AIFGQPLKRIQAWVQGK-----VPQFGEMV 231
W +A+ + P+ V V Q + G L+ + V K + +M+
Sbjct 180 EWAEAAGHTITELFPTEVPVTSGEPFIKQKTLQGLSLRDVAVSVLNKKGKPIITHKMDML 239
Query 232 ISHYGLEGGLVYRCNRDLREAMQNQNSTVLYLDLLPDQRVEQILQK----LQQGKKQSLN 287
+H+GL G + RC++ + + ++ Q + +DL PD E + QK L++ K+++
Sbjct 240 FTHFGLSGPAILRCSQFVVKELKKQPQVPIRIDLYPDINEETLFQKMYKELKEAPKKTIK 299
Query 288 TLWQKIGLDKTKIALV--RDVVDKADWNDAKK-----MAQHIKQLAINIHGFRPIEEAIS 340
+ + ++ + L+ + +++ K + KQ + +G +++A
Sbjct 300 NVLKPWMQERYLLFLLEKNGISPNVSFSELPKDPFRQFVRDCKQFTVLANGTLSLDKAFV 359
Query 341 CAGGISLDALD-QHLKLKHTSAIFACGEMLDWDAPTGGYLLTACLATGRI 389
GG+S+ +D + + K ++ CGE+LD TGGY +T+ L TGR+
Sbjct 360 TGGGVSVKEIDPKKMASKKMEGLYFCGEILDIHGYTGGYNITSALVTGRL 409
>Q18164 Dihydropyrimidine dehydrogenase [NADP(+)] [Caenorhabditis
elegans]
Length=1059
Score = 45.8 bits (107), Expect = 6e-08
Identities = 18/33 (55%), Positives = 27/33 (82%), Gaps = 0/33 (0%)
Query 4 KQVAIIGAGPAGLACAEMLSELGHYDITVYEQK 36
+QVA+IG GPA ++CA L+ LG+ DIT+YE++
Sbjct 201 EQVALIGCGPASISCASFLARLGYTDITIYEKR 233
Score = 22.3 bits (46), Expect = 1.1
Identities = 28/120 (23%), Positives = 46/120 (38%), Gaps = 25/120 (21%)
Query 123 DRGVKFRYRHQVVNVKDDQLTVLDMVQQQGHTLKVDAIVLA---CGALSWPQLGSDGKWQ 179
D G + Q+V +K D + + G TLK DA++ A C W + D Q
Sbjct 429 DNGKWYEDEEQIVILKCDYV-----ISAFGSTLKEDAVLSALQPCQLNKWGGIEVDSTTQ 483
Query 180 ----SWLDAS------VMQPFQPSNVGVLVNWS--DYLQAIFGQPLKRIQAWVQGKVPQF 227
W+ A + N G + W+ Y+Q++ G + ++PQF
Sbjct 484 QTSEKWVFAGGDVAGVAETTVESVNDGKIAAWNMHRYIQSLHGNQVSETP-----ELPQF 538
Score = 21.6 bits (44), Expect = 2.0
Identities = 9/32 (28%), Positives = 16/32 (50%), Gaps = 0/32 (0%)
Query 5 QVAIIGAGPAGLACAEMLSELGHYDITVYEQK 36
+V ++GAG + CA G +T+ +K
Sbjct 348 RVVVLGAGDTAMDCATSALRCGASRVTIAFRK 379
>A8XKG6 Probable dihydropyrimidine dehydrogenase [NADP(+)] [Caenorhabditis
briggsae]
Length=1053
Score = 45.4 bits (106), Expect = 8e-08
Identities = 17/33 (52%), Positives = 27/33 (82%), Gaps = 0/33 (0%)
Query 4 KQVAIIGAGPAGLACAEMLSELGHYDITVYEQK 36
+Q+A+IG GPA ++CA L+ LG+ DIT+YE++
Sbjct 201 EQIALIGCGPASISCASFLARLGYTDITIYEKR 233
Score = 21.6 bits (44), Expect = 2.0
Identities = 9/32 (28%), Positives = 16/32 (50%), Gaps = 0/32 (0%)
Query 5 QVAIIGAGPAGLACAEMLSELGHYDITVYEQK 36
+V ++GAG + CA G +T+ +K
Sbjct 348 RVVVLGAGDTAMDCATSALRCGASRVTIAFRK 379
>Q8U195 Sulfide dehydrogenase subunit alpha [Pyrococcus furiosus
(strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)]
Length=474
Score = 43.9 bits (102), Expect = 2e-07
Identities = 20/31 (65%), Positives = 27/31 (87%), Gaps = 1/31 (3%)
Query 4 KQVAIIGAGPAGLACAEMLSELGHYDITVYE 34
K+VAIIGAGPAGL CA L+++G Y++T+YE
Sbjct 155 KKVAIIGAGPAGLTCAADLAKMG-YEVTIYE 184
Score = 21.6 bits (44), Expect = 1.8
Identities = 17/55 (31%), Positives = 25/55 (45%), Gaps = 6/55 (11%)
Query 4 KQVAIIGAGPAGLACAEMLSELGHYDITVYEQKPSAARKFLMAGKTGLNISHAED 58
K+VA+IG G + A LG +Y + RK + A + I HAE+
Sbjct 294 KRVAVIGGGNTAMDAARSALRLGAEVWILYRR----TRKEMTAREE--EIKHAEE 342
>P37127 Putative oxidoreductase AegA [Escherichia coli (strain
K12)]
Length=659
Score = 43.9 bits (102), Expect = 2e-07
Identities = 21/35 (60%), Positives = 29/35 (83%), Gaps = 1/35 (3%)
Query 3 NKQVAIIGAGPAGLACAEMLSELGHYDITVYEQKP 37
+K+VAIIGAGPAGLACA++L+ G +TVY++ P
Sbjct 327 DKRVAIIGAGPAGLACADVLTRNG-VGVTVYDRHP 360
>Q74FU5 NADPH-Fe(3+) oxidoreductase subunit beta [Geobacter sulfurreducens
(strain ATCC 51573 / DSM 12127 / PCA)]
Length=672
Score = 43.1 bits (100), Expect = 4e-07
Identities = 22/35 (63%), Positives = 26/35 (74%), Gaps = 1/35 (3%)
Query 3 NKQVAIIGAGPAGLACAEMLSELGHYDITVYEQKP 37
NK+VAI+GAGPAGLACA L+ L Y T+YE P
Sbjct 253 NKKVAIVGAGPAGLACAYYLA-LEGYPCTIYEALP 286
>D7AF64 NADPH-Fe(3+) oxidoreductase subunit beta [Geobacter sulfurreducens
(strain DL-1 / KN400)]
Length=672
Score = 43.1 bits (100), Expect = 4e-07
Identities = 22/35 (63%), Positives = 26/35 (74%), Gaps = 1/35 (3%)
Query 3 NKQVAIIGAGPAGLACAEMLSELGHYDITVYEQKP 37
NK+VAI+GAGPAGLACA L+ L Y T+YE P
Sbjct 253 NKKVAIVGAGPAGLACAYYLA-LEGYPCTIYEALP 286
>Q8ZNL8 NAD-dependent dihydropyrimidine dehydrogenase subunit
PreT [Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)]
Length=413
Score = 42.7 bits (99), Expect = 4e-07
Identities = 20/33 (61%), Positives = 27/33 (82%), Gaps = 1/33 (3%)
Query 5 QVAIIGAGPAGLACAEMLSELGHYDITVYEQKP 37
+VAIIGAGPAGL + L+ LG YD+T+YE++P
Sbjct 126 KVAIIGAGPAGLQASVTLTHLG-YDVTIYEKQP 157
Score = 20.8 bits (42), Expect = 3.2
Identities = 13/49 (27%), Positives = 21/49 (43%), Gaps = 5/49 (10%)
Query 350 LDQHLKLKHTSAIFACGEMLDWDAPTGGYLLTACLATGRITAHGVHAQL 398
+D H AIFA G+++ G + + TG+ A +H L
Sbjct 364 IDTHNYQTDDPAIFAAGDIV-----KGDKTVVYAVKTGKEAAQAIHHYL 407
>Q8Z5A6 NAD-dependent dihydropyrimidine dehydrogenase subunit
PreT homolog [Salmonella typhi]
Length=413
Score = 42.7 bits (99), Expect = 4e-07
Identities = 20/33 (61%), Positives = 27/33 (82%), Gaps = 1/33 (3%)
Query 5 QVAIIGAGPAGLACAEMLSELGHYDITVYEQKP 37
+VAIIGAGPAGL + L+ LG YD+T+YE++P
Sbjct 126 KVAIIGAGPAGLQASVTLTHLG-YDVTIYEKQP 157
Score = 20.8 bits (42), Expect = 3.2
Identities = 13/49 (27%), Positives = 21/49 (43%), Gaps = 5/49 (10%)
Query 350 LDQHLKLKHTSAIFACGEMLDWDAPTGGYLLTACLATGRITAHGVHAQL 398
+D H AIFA G+++ G + + TG+ A +H L
Sbjct 364 IDTHNYQTDDPAIFAAGDIV-----KGDKTVVYAVKTGKEAAQAIHHYL 407
>Q0JKD0 Glutamate synthase 1 [NADH], chloroplastic [Oryza sativa
subsp. japonica]
Length=2167
Score = 43.1 bits (100), Expect = 4e-07
Identities = 19/32 (59%), Positives = 29/32 (91%), Gaps = 1/32 (3%)
Query 4 KQVAIIGAGPAGLACAEMLSELGHYDITVYEQ 35
K+VAIIG+GPAGLA A+ L+++GH+ +TV+E+
Sbjct 1807 KKVAIIGSGPAGLAAADQLNKMGHF-VTVFER 1837
>Q9LV03 Glutamate synthase 1 [NADH], chloroplastic [Arabidopsis
thaliana]
Length=2208
Score = 43.1 bits (100), Expect = 4e-07
Identities = 20/32 (63%), Positives = 28/32 (88%), Gaps = 1/32 (3%)
Query 4 KQVAIIGAGPAGLACAEMLSELGHYDITVYEQ 35
K+VAIIG+GPAGLA A+ L+++GH +TVYE+
Sbjct 1846 KKVAIIGSGPAGLAAADQLNKMGHL-VTVYER 1876
>Q12680 Glutamate synthase [NADH] [Saccharomyces cerevisiae (strain
ATCC 204508 / S288c)]
Length=2145
Score = 42.7 bits (99), Expect = 6e-07
Identities = 19/30 (63%), Positives = 25/30 (83%), Gaps = 1/30 (3%)
Query 6 VAIIGAGPAGLACAEMLSELGHYDITVYEQ 35
V +IG+GPAGLACA+ML+ GH +TVYE+
Sbjct 1785 VGVIGSGPAGLACADMLNRAGH-TVTVYER 1813
Score = 23.9 bits (50), Expect = 0.39
Identities = 17/57 (30%), Positives = 24/57 (42%), Gaps = 4/57 (7%)
Query 345 ISLDALDQHLKLKHTSAIFACGEMLDWDAPTGGYLLTACLATGRITAHGVHAQLNAD 401
IS+D LK KH + ++A G + D P G L ++ A LN D
Sbjct 1859 ISMD----ELKNKHNAVVYAIGSTIPRDLPIKGRELKNIDFAMQLLESNTKALLNKD 1911
Score = 20.8 bits (42), Expect = 3.5
Identities = 10/26 (38%), Positives = 13/26 (50%), Gaps = 0/26 (0%)
Query 142 LTVLDMVQQQGHTLKVDAIVLACGAL 167
L DM+ + GHT+ V CG L
Sbjct 1795 LACADMLNRAGHTVTVYERSDRCGGL 1820
>Q0DG35 Glutamate synthase 2 [NADH], chloroplastic [Oryza sativa
subsp. japonica]
Length=2188
Score = 42.7 bits (99), Expect = 6e-07
Identities = 18/32 (56%), Positives = 29/32 (91%), Gaps = 1/32 (3%)
Query 4 KQVAIIGAGPAGLACAEMLSELGHYDITVYEQ 35
K+VAI+G+GPAGLA A+ L+++GH+ +TV+E+
Sbjct 1825 KRVAIVGSGPAGLAAADQLNKMGHF-VTVFER 1855
>Q28943 Dihydropyrimidine dehydrogenase [NADP(+)] [Sus scrofa]
Length=1025
Score = 42.4 bits (98), Expect = 7e-07
Identities = 15/32 (47%), Positives = 27/32 (84%), Gaps = 0/32 (0%)
Query 5 QVAIIGAGPAGLACAEMLSELGHYDITVYEQK 36
++A++GAGPA ++CA L+ LG+ DIT++E++
Sbjct 189 KIALLGAGPASISCASFLARLGYSDITIFEKQ 220
Score = 20.4 bits (41), Expect = 4.5
Identities = 8/19 (42%), Positives = 12/19 (63%), Gaps = 0/19 (0%)
Query 210 GQPLKRIQAWVQGKVPQFG 228
G+P+ RI + K+P FG
Sbjct 862 GKPVPRIAELMGKKLPNFG 880
Score = 20.0 bits (40), Expect = 5.9
Identities = 18/67 (27%), Positives = 28/67 (42%), Gaps = 13/67 (19%)
Query 6 VAIIGAGPAGLACAEMLSELGHYDITVYEQKPSAARKFLMAGKTGLNISHAEDLEIFIAR 65
V ++GAG CA G A R FL+ K +NI A E+ +A+
Sbjct 335 VIVLGAGDTAFDCATSALRCG------------ARRVFLVFRKGFVNI-RAVPEEVELAK 381
Query 66 YDHADWL 72
+ ++L
Sbjct 382 EEKCEFL 388
>Q28007 Dihydropyrimidine dehydrogenase [NADP(+)] [Bos taurus]
Length=1025
Score = 42.4 bits (98), Expect = 7e-07
Identities = 15/32 (47%), Positives = 27/32 (84%), Gaps = 0/32 (0%)
Query 5 QVAIIGAGPAGLACAEMLSELGHYDITVYEQK 36
++A++GAGPA ++CA L+ LG+ DIT++E++
Sbjct 189 KIALLGAGPASISCASFLARLGYNDITIFEKQ 220
Score = 20.4 bits (41), Expect = 4.5
Identities = 18/67 (27%), Positives = 28/67 (42%), Gaps = 13/67 (19%)
Query 6 VAIIGAGPAGLACAEMLSELGHYDITVYEQKPSAARKFLMAGKTGLNISHAEDLEIFIAR 65
V ++GAG CA G A R F++ K +NI A E+ +AR
Sbjct 335 VIVLGAGDTAFDCATSALRCG------------ARRVFIVFRKGFVNI-RAVPEEVELAR 381
Query 66 YDHADWL 72
+ ++L
Sbjct 382 EEKCEFL 388
>O89000 Dihydropyrimidine dehydrogenase [NADP(+)] [Rattus norvegicus]
Length=1025
Score = 41.6 bits (96), Expect = 1e-06
Identities = 15/32 (47%), Positives = 26/32 (81%), Gaps = 0/32 (0%)
Query 5 QVAIIGAGPAGLACAEMLSELGHYDITVYEQK 36
++A+ GAGPA ++CA L+ LG+ DIT++E++
Sbjct 189 KIALFGAGPASISCASFLARLGYSDITIFEKQ 220
Score = 20.8 bits (42), Expect = 3.4
Identities = 14/50 (28%), Positives = 21/50 (42%), Gaps = 8/50 (16%)
Query 296 DKTKIALVRDVVDKADWNDAKKMAQHIKQLAINIHGFRPIEEAISCAGGI 345
D IA + +K DW + KMA+ G +E +SC G+
Sbjct 634 DNILIASIMCSYNKNDWMELSKMAE--------ASGADALELNLSCPHGM 675
>Q12882 Dihydropyrimidine dehydrogenase [NADP(+)] [Homo sapiens]
Length=1025
Score = 41.6 bits (96), Expect = 1e-06
Identities = 15/32 (47%), Positives = 26/32 (81%), Gaps = 0/32 (0%)
Query 5 QVAIIGAGPAGLACAEMLSELGHYDITVYEQK 36
++A+ GAGPA ++CA L+ LG+ DIT++E++
Sbjct 189 KIALFGAGPASISCASFLARLGYSDITIFEKQ 220
Score = 21.2 bits (43), Expect = 2.6
Identities = 8/19 (42%), Positives = 12/19 (63%), Gaps = 0/19 (0%)
Query 210 GQPLKRIQAWVQGKVPQFG 228
G+P+ RI + K+P FG
Sbjct 862 GKPVPRIAELMDKKLPSFG 880
Lambda K H a alpha
0.323 0.137 0.424 0.792 4.96
Gapped
Lambda K H a alpha sigma
0.267 0.0410 0.140 1.90 42.6 43.6
Effective search space used: 6074008
Database: 47a4e1224d602a0b9f95b476187b97cd.SwissProt.fasta
Posted date: May 10, 2024 9:22 PM
Number of letters in database: 18,643
Number of sequences in database: 17
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 11
Window for multiple hits: 40
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Data ...
