BLASTP 2.12.0+
Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics: Alejandro A. Schaffer,
L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri
I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids
Res. 29:2994-3005.
Database: c76581917da3a8e1413f1123763298c8.SwissProt.fasta
1 sequences; 3,114 total letters
Query= ACIAD0489
Length=335
Score E
Sequences producing significant alignments: (Bits) Value
P49454 Centromere protein F [Homo sapiens] 37.4 3e-06
>P49454 Centromere protein F [Homo sapiens]
Length=3114
Score = 37.4 bits (85), Expect = 3e-06
Identities = 38/169 (22%), Positives = 82/169 (49%), Gaps = 11/169 (7%)
Query 35 ENKLECALDELTELSSWISSGQVIILDVPKIKDRASAYALIAGQLLAL-IEPEANIEII- 92
+ KLE +E++ L + I + ++ + +++ + +L L +E E I+++
Sbjct 2514 KQKLEKKDEEISRLKNQIQDQEQLVSKLSQVEGEHQLWKEQNLELRNLTVELEQKIQVLQ 2573
Query 93 SKNTAVQQLLEILQASGKVVEQ--VLPQINQKALCHLPSMQSFNEQPMLREIKKYCDAVG 150
SKN ++Q LE+LQ+S K +E L ++++ + + + E + RE+ +
Sbjct 2574 SKNASLQDTLEVLQSSYKNLENELELTKMDKMSFVEKVNKMTAKETELQREMHEMAQKTA 2633
Query 151 KTMKGIPLTVEGLKNSIANILQLSPAQVPQIMSMLINLKIIKRDQTQIK 199
+ + + G KN +A LQL + +I S LK + + +++K
Sbjct 2634 ELQE----ELSGEKNRLAGELQL---LLEEIKSSKDQLKELTLENSELK 2675
Score = 21.9 bits (45), Expect = 0.21
Identities = 36/168 (21%), Positives = 72/168 (43%), Gaps = 14/168 (8%)
Query 78 QLLALIEPEANIEIISKNTAVQQLLEILQASGKVVEQVLPQI---NQKALCHLPSMQSFN 134
Q L +E + + I KN +Q+L ++L + + ++ + Q N++ L S+
Sbjct 1561 QSLEKLEEKMESQGIMKNKEIQELEQLLSSERQELDCLRKQYLSENEQWQQKLTSVTLEM 1620
Query 135 EQPMLREIKKYCDAVGKTMKGIPLTVEGLKNSIANILQLSPAQVPQIMSMLINLKIIKRD 194
E + E KK + + ++ L ++GL S ++L + Q + ++
Sbjct 1621 ESKLAAE-KKQTEQLSLELEVARLQLQGLDLSSRSLLGIDTEDAIQGRNESCDISKEHTS 1679
Query 195 QTQIKFRK--------KILKQWLNLEL--IEQEIAIQPAQVQSVTQQP 232
+T + K K +Q LNL++ I + A++P S Q P
Sbjct 1680 ETTERTPKHDVHQICDKDAQQDLNLDIEKITETGAVKPTGECSGEQSP 1727
Score = 20.4 bits (41), Expect = 0.63
Identities = 8/13 (62%), Positives = 10/13 (77%), Gaps = 0/13 (0%)
Query 36 NKLECALDELTEL 48
+KLEC L+E T L
Sbjct 1051 SKLECLLNECTSL 1063
Score = 20.4 bits (41), Expect = 0.63
Identities = 13/39 (33%), Positives = 21/39 (54%), Gaps = 2/39 (5%)
Query 2 KSIILIDLEKNPIQANSLRLYIENEQLIYLFHPENKLEC 40
+ I+ + E+N +Q L ENEQL+ + + K EC
Sbjct 1130 QEIMTLKEEQNKMQKEVNDLLQENEQLMKVM--KTKHEC 1166
Score = 19.6 bits (39), Expect = 1.1
Identities = 19/95 (20%), Positives = 44/95 (46%), Gaps = 1/95 (1%)
Query 161 EGLKNSIANILQLSPAQVPQIMSMLINLKIIKRDQTQIKFRKKIL-KQWLNLELIEQEIA 219
E L NS+ Q+ M L + +K++ + + + K+L K LE ++ +
Sbjct 2754 EELNNSLKATTQILEELKKTKMDNLKYVNQLKKENERAQGKMKLLIKSCKQLEEEKEILQ 2813
Query 220 IQPAQVQSVTQQPAIAGLPHVVVGDVDSIMQFLKE 254
+ +Q+Q+ ++ + V ++ + ++ LKE
Sbjct 2814 KELSQLQAAQEKQKTGTVMDTKVDELTTEIKELKE 2848
Score = 19.2 bits (38), Expect = 1.4
Identities = 23/100 (23%), Positives = 48/100 (48%), Gaps = 9/100 (9%)
Query 38 LECALDELTELSSWISSGQVIILDVPK----IKDRASAYALIAGQLLALIEPEANIEIIS 93
LE EL L+ + + Q I ++ K ++D L++ L +E + IIS
Sbjct 906 LENKEKELQLLNDKVETEQAEIQELKKSNHLLEDSLKELQLLSETLS--LEKKEMSSIIS 963
Query 94 KNTAVQQLLEILQASGKVVEQVLPQINQKALCHLPSMQSF 133
N +++ E+ Q +G ++++ +NQ+ + + +SF
Sbjct 964 LNK--REIEELTQENG-TLKEINASLNQEKMNLIQKSESF 1000
Score = 18.1 bits (35), Expect = 3.3
Identities = 8/15 (53%), Positives = 12/15 (80%), Gaps = 0/15 (0%)
Query 266 SLDELQWQALQKLDE 280
SL E+ Q+L+KL+E
Sbjct 1554 SLCEVYRQSLEKLEE 1568
Score = 17.7 bits (34), Expect = 4.3
Identities = 13/46 (28%), Positives = 23/46 (50%), Gaps = 8/46 (17%)
Query 271 QWQALQKLDELKLGRPKDIFSLRDMLQDWFPQA-NIQNLLKALMDK 315
Q ++ Q LD+ + ++ L QA N+ N+L+A +DK
Sbjct 403 QQRSFQTLDQ-------ECIQMKARLTQELQQAKNMHNVLQAELDK 441
Score = 16.5 bits (31), Expect = 9.7
Identities = 48/238 (20%), Positives = 95/238 (40%), Gaps = 32/238 (13%)
Query 7 IDLEKNPIQANSLRLYIENEQLIYLFHPENKLECALDELTELSSWISSGQVIILDVPKIK 66
+D+E + S + IE+E L YL E LE E L + Q +I+ ++
Sbjct 1898 LDVENELSRIRSEKASIEHEAL-YL---EADLEVVQTEKLCLEKDNENKQKVIV---CLE 1950
Query 67 DRASAYALIAGQLLALIEPEANIEIISKNTAVQQLLEILQASGKVVEQ---------VLP 117
+ S QL ++ + K TA+ QL E ++ + +E +
Sbjct 1951 EELSVVTSERNQLRGELD-----TMSKKTTALDQLSEKMKEKTQELESHQSECLHCIQVA 2005
Query 118 QINQKALCHLPSMQSFNEQPMLRE---IKKYCDAVGKTMKGIPLTVEGLKNSIANILQLS 174
+ K L S + +L++ +++ ++ K + + LT L+N IA + +
Sbjct 2006 EAEVKEKTELLQTLSSDVSELLKDKTHLQEKLQSLEKDSQALSLTKCELENQIAQLNKEK 2065
Query 175 PAQVPQIMSMLINLKIIKRDQTQIKFRKKILKQWLNLELIEQ-EIAIQPAQVQSVTQQ 231
V + S+ L ++ + K + + L L+E+ E A++ + Q Q
Sbjct 2066 ELLVKESESLQARL-------SESDYEKLNVSKALEAALVEKGEFALRLSSTQEEVHQ 2116
Lambda K H a alpha
0.321 0.137 0.391 0.792 4.96
Gapped
Lambda K H a alpha sigma
0.267 0.0410 0.140 1.90 42.6 43.6
Effective search space used: 893370
Database: c76581917da3a8e1413f1123763298c8.SwissProt.fasta
Posted date: May 20, 2024 8:11 AM
Number of letters in database: 3,114
Number of sequences in database: 1
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 11
Window for multiple hits: 40
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Data ...
