BLASTP 2.12.0+
Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics: Alejandro A. Schaffer,
L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri
I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids
Res. 29:2994-3005.
Database: 8b6f7ee67837ce4f613c257e928a18d7.SwissProt.fasta
10 sequences; 3,316 total letters
Query= ACIAD1060
Length=277
Score E
Sequences producing significant alignments: (Bits) Value
P46116 Rhomboid protease AarA [Providencia stuartii] 91.3 4e-25
P54493 Rhomboid protease GluP [Bacillus subtilis (strain 168)] 80.5 1e-20
Q8VZ48 RHOMBOID-like protein 6, mitochondrial [Arabidopsis thaliana] 72.8 2e-18
Q9CAN1 RHOMBOID-like protein 2 [Arabidopsis thaliana] 69.7 3e-17
Q9SSR0 RHOMBOID-like protein 5 [Arabidopsis thaliana] 68.6 7e-17
F4ICF4 RHOMBOID-like protein 10, chloroplastic [Arabidopsis thali... 67.4 2e-16
O82756 RHOMBOID-like protein 7 [Arabidopsis thaliana] 66.2 4e-16
Q9LYP1 RHOMBOID-like protein 3 [Arabidopsis thaliana] 65.9 7e-16
F4JBM4 RHOMBOID-like protein 4 [Arabidopsis thaliana] 65.1 2e-15
P96617 Putative rhomboid protease YdcA [Bacillus subtilis (strain... 57.8 1e-13
>P46116 Rhomboid protease AarA [Providencia stuartii]
Length=281
Score = 91.3 bits (225), Expect = 4e-25
Identities = 70/251 (28%), Positives = 122/251 (49%), Gaps = 17/251 (7%)
Query 12 KLDLHAWWMTAMLIAINVGLFVWQILHGVDASNPAPADAIRWGADFAPLTYLEQPQRLIT 71
+ L A +T L+ +N+ ++ +QI+ + ++ I +GA+ L+ R
Sbjct 15 RFSLGAIALTLTLVLLNIAVYFYQIVFASPLDS-RESNLILFGANIYQLSLTGDWWRYPI 73
Query 72 SMFFHFGFVHLALNMWALYIFGSLAEQILGRFYFIGLYFLAGLMGSLLSGYMSIQDSYEL 131
SM H HLA N AL++ G E+ G+F + +Y ++G+ +L S Y YE+
Sbjct 74 SMMLHSNGTHLAFNCLALFVIGIGCERAYGKFKLLAIYIISGIGAALFSAYWQY---YEI 130
Query 132 LNHFNSIAGSQSSLIPHIAAGASGAVMGLGGALTILAFF--------PPLPLQRFILDKK 183
NS + S++ I GASGA+MG+ A I P+ +R
Sbjct 131 ---SNSDLWTDSTVYITIGVGASGAIMGIAAASVIYLIKVVINKPNPHPVIQRRQKYQLY 187
Query 184 ALLIVMGINLAFGFMTIGINNSAHIGGMIMGAFLVFIWYLFQKMKAQMLGNLIGLVSGSL 243
L+ ++ + L G + G++N+AHIGG I+GA L+ I Y+ K ++ I +++ SL
Sbjct 188 NLIAMIALTLINGLQS-GVDNAAHIGGAIIGA-LISIAYILVPHKLRVANLCITVIAASL 245
Query 244 VCVVFYQYCIS 254
+ ++ Y Y S
Sbjct 246 LTMMIYLYSFS 256
>P54493 Rhomboid protease GluP [Bacillus subtilis (strain 168)]
Length=507
Score = 80.5 bits (197), Expect = 1e-20
Identities = 60/196 (31%), Positives = 91/196 (46%), Gaps = 27/196 (14%)
Query 21 TAMLIAINVGLFVWQILHGVDASNPAPADAIRWGADFAPLTYLEQPQRLITSMFFHFGFV 80
T + IA+ + +F ++G + + +GA L + RL+T + H G
Sbjct 180 TYLFIALQILMFFLLEINGGSTNTET---LVAFGAKENSLIAQGEWWRLLTPIVLHIGIA 236
Query 81 HLALNMWALYIFGSLAEQILGRFYFIGLYFLAGLMGSLLSGYMSIQDSYELLNHFNSIAG 140
HLA N AL+ G+ E++ G F+ +Y AG+ GS+ S S
Sbjct 237 HLAFNTLALWSVGTAVERMYGSGRFLLIYLAAGITGSIASFVFS---------------- 280
Query 141 SQSSLIPHIAAGASGAVMGLGGALTILAFFPPLPLQRFILDKKALLIVMGINLAFGFMTI 200
P+ +AGASGA+ G GAL +A R I +++++ INL FGF
Sbjct 281 ------PYPSAGASGAIFGCLGALLYVALSNRKMFLRTI--GTNIIVIIIINLGFGFAVS 332
Query 201 GINNSAHIGGMIMGAF 216
I+NS HIGG+I G F
Sbjct 333 NIDNSGHIGGLIGGFF 348
>Q8VZ48 RHOMBOID-like protein 6, mitochondrial [Arabidopsis thaliana]
Length=307
Score = 72.8 bits (177), Expect = 2e-18
Identities = 62/191 (32%), Positives = 94/191 (49%), Gaps = 25/191 (13%)
Query 32 FVWQILHGVDASNPAPADAIRWGA-DFAPLTYLEQPQRLITSMFFHFGFVHLALNMWALY 90
F +Q L P+ + + GA D+ + + RLIT+M+ H G +HL +NM+ +
Sbjct 62 FSFQPLRENPFLGPSSSTLEKLGALDWKKVVQGNEKWRLITAMWLHAGIIHLVMNMFDVI 121
Query 91 IFGSLAEQILGRFYFIGL-YFLAGLMGSLLSGYMSIQDSYELLNHFNSIAGSQSSLIPHI 149
IFG EQ G F IGL Y ++G GS+LS + +Q S I
Sbjct 122 IFGIRLEQQFG-FIRIGLIYLISGFGGSILSA-LFLQKS--------------------I 159
Query 150 AAGASGAVMGLGGALTILAFFPPLPLQRFILDKKALLIVMGINLAFGFMTIGINNSAHIG 209
+ GASGA++GL GA+ + + + L ++ INLA G + ++N AHIG
Sbjct 160 SVGASGALLGLMGAMLSELLTNWTIYKSKLCALLSFLFIIAINLAIGLLP-WVDNFAHIG 218
Query 210 GMIMGAFLVFI 220
G++ G L FI
Sbjct 219 GLLTGFCLGFI 229
Score = 16.5 bits (31), Expect = 7.6
Identities = 13/36 (36%), Positives = 16/36 (44%), Gaps = 2/36 (6%)
Query 1 MSELTPPQINRKLDLHAWWMTAMLIAIN--VGLFVW 34
+SEL K L A +IAIN +GL W
Sbjct 175 LSELLTNWTIYKSKLCALLSFLFIIAINLAIGLLPW 210
>Q9CAN1 RHOMBOID-like protein 2 [Arabidopsis thaliana]
Length=317
Score = 69.7 bits (169), Expect = 3e-17
Identities = 66/203 (33%), Positives = 97/203 (48%), Gaps = 35/203 (17%)
Query 25 IAINVGLFVWQILHGVDASNPAPADAIRWGA-DFAPLTYLEQPQRLITSMFFHFGFVHLA 83
+A +G F +Q L P+ + + GA ++ + + Q RL++ M+ H G +HL
Sbjct 68 VARFLGRFSFQPLKENPLFGPSSSTLEKMGALEWRKVVHEHQGWRLLSCMWLHAGIIHLL 127
Query 84 LNMWALYIFGSLAEQILGRFYFIGL-YFLAGLMGSLLSGYMSIQDSYELLNHFNSIAGSQ 142
NM +L G EQ G F +GL Y ++GL GS+LS + +Q+S
Sbjct 128 TNMLSLIFIGIRLEQQFG-FIRVGLIYLISGLGGSILSS-LFLQES-------------- 171
Query 143 SSLIPHIAAGASGAVMGLGGALTILAFFPPLPLQRFILDKKA-----LLIVMGINLAFGF 197
I+ GASGA+ GL GA+ L I KA LL ++ INLA G
Sbjct 172 ------ISVGASGALFGLLGAM-----LSELLTNWTIYANKAAALITLLFIIAINLALG- 219
Query 198 MTIGINNSAHIGGMIMGAFLVFI 220
M ++N AHIGG + G L F+
Sbjct 220 MLPRVDNFAHIGGFLTGFCLGFV 242
Score = 17.7 bits (34), Expect = 3.3
Identities = 13/42 (31%), Positives = 22/42 (52%), Gaps = 3/42 (7%)
Query 217 LVFIWYLFQKMKAQMLGNLIGLVS---GSLVCVVFYQYCISL 255
L+FI ++ + LI L+S GS++ +F Q IS+
Sbjct 133 LIFIGIRLEQQFGFIRVGLIYLISGLGGSILSSLFLQESISV 174
>Q9SSR0 RHOMBOID-like protein 5 [Arabidopsis thaliana]
Length=309
Score = 68.6 bits (166), Expect = 7e-17
Identities = 50/156 (32%), Positives = 75/156 (48%), Gaps = 20/156 (13%)
Query 68 RLITSMFFHFGFVHLALNMWALYIFGSLAEQILGRFYFIGLYFLAGLMGSLLSGYMSIQD 127
RLI+ ++ H GF+HL NM +L G EQ G LY ++GL GSL+S
Sbjct 108 RLISCIWLHGGFLHLMANMISLMCIGMRLEQEFGFMRIGALYVISGLGGSLVS------- 160
Query 128 SYELLNHFNSIAGSQSSLIPHIAAGASGAVMGLGGALTILAFFPPLPLQRFILDKKALLI 187
+ SQ ++ GASGA+ GL GA+ + L++
Sbjct 161 ---------CLTDSQGE---RVSVGASGALFGLLGAMLSELITNWTIYENKCTALMTLIL 208
Query 188 VMGINLAFGFMTIGINNSAHIGGMIMGAFLVFIWYL 223
++ +NL+ GF+ ++NSAH GG + G FL F+ L
Sbjct 209 IIVLNLSVGFLP-RVDNSAHFGGFLAGFFLGFVLLL 243
>F4ICF4 RHOMBOID-like protein 10, chloroplastic [Arabidopsis thaliana]
Length=343
Score = 67.4 bits (163), Expect = 2e-16
Identities = 52/214 (24%), Positives = 96/214 (45%), Gaps = 23/214 (11%)
Query 7 PQINRKLDLHAWWMTAMLIAINVGLFVWQILHGVDASNPAPADAIRWGADFAPLTYLEQP 66
P+ +++ ++ T +L+AINV +++ QI + + WGA L Q
Sbjct 116 PETSKRNTVNGRRWTNVLLAINVIMYIAQIA--------SDGKVLTWGAKINSLIERGQL 167
Query 67 QRLITSMFFHFGFVHLALNMWALYIFGSLAEQILGRFYFIGLYFLAGLMGSLLSGYMSIQ 126
RL T+ H +HL +N ++L G AE + G F+ +Y + + +L S
Sbjct 168 WRLATASVLHANPMHLMINCYSLNSIGPTAESLGGPKRFLAVYLTSAVAKPILRVLGSAM 227
Query 127 DSYELLNHFNSIAGSQSSLIPHIAAGASGAVMGLGGALTILAFFPPLPLQRFILDKKALL 186
+ FN + GASGA+ GL G++ + ++ D +
Sbjct 228 SYW-----FNKAP----------SVGASGAIFGLVGSVAVFVIRHKQMVRGGNEDLMQIA 272
Query 187 IVMGINLAFGFMTIGINNSAHIGGMIMGAFLVFI 220
++ +N+A G M+ I+N HIGG++ G + ++
Sbjct 273 QIIALNMAMGLMSRRIDNWGHIGGLLGGTAMTWL 306
>O82756 RHOMBOID-like protein 7 [Arabidopsis thaliana]
Length=313
Score = 66.2 bits (160), Expect = 4e-16
Identities = 59/213 (28%), Positives = 95/213 (45%), Gaps = 38/213 (18%)
Query 37 LHGVDASNPAPADAIRWGADFAPLTYLEQPQRLITSMFFHFGFVHLALNMWALYIFGSLA 96
L G +S A+ WG + + Q RL+T M+ H G +HL NM + G
Sbjct 79 LLGPSSSTLEKMGALAWGK----IVHKRQVWRLLTCMWLHAGVIHLLANMCCVAYIGVRL 134
Query 97 EQILGRFYFIGLYFLAGLMGSLLSGYMSIQDSYELLNHFNSIAGSQSSLIPHIAAGASGA 156
EQ G +Y ++G GS+LS + ++D+ I+ GAS A
Sbjct 135 EQQFGFVRVGTIYLVSGFCGSILS-CLFLEDA--------------------ISVGASSA 173
Query 157 VMGLGGALTILAFFPPLPLQRFILDKKA-----LLIVMGINLAFGFMTIGINNSAHIGGM 211
+ GL GA+ L + D K LL+++G+NL G + ++N AHIGG
Sbjct 174 LFGLLGAM-----LSELLINWTTYDNKGVAIVMLLVIVGVNLGLGTLP-PVDNFAHIGGF 227
Query 212 IMGAFLVFIWYLFQKMKAQMLGNLIGLVSGSLV 244
G L F+ + + + + N + L+ G++V
Sbjct 228 FGGFLLGFLLLIHPQFEWE--ENQVSLMPGTIV 258
>Q9LYP1 RHOMBOID-like protein 3 [Arabidopsis thaliana]
Length=346
Score = 65.9 bits (159), Expect = 7e-16
Identities = 54/154 (35%), Positives = 76/154 (49%), Gaps = 24/154 (16%)
Query 68 RLITSMFFHFGFVHLALNMWALYIFGSLAEQILGRFYFIG-LYFLAGLMGSLLSGYMSIQ 126
RL+T ++ H G +HL NM +L G EQ G F IG +Y L+G+ GS+LS + I+
Sbjct 136 RLLTCIWLHAGVIHLGANMLSLVFIGIRLEQQFG-FVRIGVIYLLSGIGGSVLSS-LFIR 193
Query 127 DSYELLNHFNSIAGSQSSLIPHIAAGASGAVMGLGGALTILAFFPPLPLQRFILDKKALL 186
+S I+ GASGA+ GL G++ F I LL
Sbjct 194 NS--------------------ISVGASGALFGLLGSMLSELFTNWTIYSNKIAALLTLL 233
Query 187 IVMGINLAFGFMTIGINNSAHIGGMIMGAFLVFI 220
V+ INLA G + ++N AH+GG + G L FI
Sbjct 234 FVILINLAIGILP-HVDNFAHVGGFVTGFLLGFI 266
Score = 16.9 bits (32), Expect = 5.8
Identities = 12/55 (22%), Positives = 29/55 (53%), Gaps = 6/55 (11%)
Query 207 HIGGMIMGAFLVFIWYLFQKMKAQMLGNLIGL------VSGSLVCVVFYQYCISL 255
H G + +GA ++ + ++ +++ Q IG+ + GS++ +F + IS+
Sbjct 144 HAGVIHLGANMLSLVFIGIRLEQQFGFVRIGVIYLLSGIGGSVLSSLFIRNSISV 198
>F4JBM4 RHOMBOID-like protein 4 [Arabidopsis thaliana]
Length=394
Score = 65.1 bits (157), Expect = 2e-15
Identities = 51/168 (30%), Positives = 76/168 (45%), Gaps = 24/168 (14%)
Query 54 GADFAPLTYLEQPQRLITSMFFHFGFVHLALNMWALYIFGSLAEQILGRFYFIG-LYFLA 112
G D + ++ RL++ + H G VHL +NM L G E+ G F IG LY ++
Sbjct 127 GLDVKKVVKGDEGWRLLSCNWLHGGVVHLLMNMLTLLFIGIRMEREFG-FIRIGLLYLIS 185
Query 113 GLMGSLLSGYMSIQDSYELLNHFNSIAGSQSSLIPHIAAGASGAVMGLGGALTILAFFPP 172
G GS+LS L +I+ GASGAV GL G + F
Sbjct 186 GFGGSILSALF---------------------LRSNISVGASGAVFGLLGGMLSEIFINW 224
Query 173 LPLQRFILDKKALLIVMGINLAFGFMTIGINNSAHIGGMIMGAFLVFI 220
++ L++++ +NL G + G++N AHIGG G L F+
Sbjct 225 TIYSNKVVTIVTLVLIVAVNLGLGVLP-GVDNFAHIGGFATGFLLGFV 271
Score = 18.5 bits (36), Expect = 1.9
Identities = 8/19 (42%), Positives = 14/19 (74%), Gaps = 3/19 (16%)
Query 23 MLIAINVGLFVWQILHGVD 41
+++A+N+GL +L GVD
Sbjct 239 LIVAVNLGL---GVLPGVD 254
>P96617 Putative rhomboid protease YdcA [Bacillus subtilis (strain
168)]
Length=199
Score = 57.8 bits (138), Expect = 1e-13
Identities = 38/153 (25%), Positives = 69/153 (45%), Gaps = 22/153 (14%)
Query 68 RLITSMFFHFGFVHLALNMWALYIFGSLAEQILGRFYFIGLYFLAGLMGSLLSGYMSIQD 127
RLIT + H GF HL N ++++F E++LG+ F+ +Y +G++G
Sbjct 60 RLITPILLHAGFTHLLFNSMSIFLFAPALERMLGKARFLLVYAGSGIIG----------- 108
Query 128 SYELLNHFNSIAGSQSSLIPHIAAGASGAVMGLGGALTILAFFPPLPLQRFILDKKALLI 187
+I + + ++ GASGA+ GL G + F + + K ++
Sbjct 109 ---------NIGTYVTEPLDYVHVGASGAIFGLFGVYLFMVLFRNELIGQ--EHSKMIIT 157
Query 188 VMGINLAFGFMTIGINNSAHIGGMIMGAFLVFI 220
++ + F+ IN AH+ G+ G L F+
Sbjct 158 LLAFAVLMSFINSNINMMAHLFGLCGGFLLSFL 190
Lambda K H a alpha
0.332 0.145 0.463 0.792 4.96
Gapped
Lambda K H a alpha sigma
0.267 0.0410 0.140 1.90 42.6 43.6
Effective search space used: 680560
Database: 8b6f7ee67837ce4f613c257e928a18d7.SwissProt.fasta
Posted date: Jun 2, 2024 5:31 AM
Number of letters in database: 3,316
Number of sequences in database: 10
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 11
Window for multiple hits: 40
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Data ...
