BLASTP 2.12.0+
Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics: Alejandro A. Schaffer,
L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri
I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids
Res. 29:2994-3005.
Database: e6c0932e827f2e1d8c613e2639573f8e.SwissProt.fasta
2 sequences; 1,122 total letters
Query= ACIAD1235
Length=90
Score E
Sequences producing significant alignments: (Bits) Value
Q3M8C8 Lysine--tRNA ligase [Trichormus variabilis (strain ATCC 29... 37.4 3e-08
Q8YPW9 Lysine--tRNA ligase [Nostoc sp. (strain PCC 7120 / SAG 25.... 37.4 3e-08
>Q3M8C8 Lysine--tRNA ligase [Trichormus variabilis (strain ATCC
29413 / PCC 7937)]
Length=561
Score = 37.4 bits (85), Expect = 3e-08
Identities = 16/60 (27%), Positives = 35/60 (58%), Gaps = 0/60 (0%)
Query 28 VKSWKYDANTRHLKIFYENGTGDLYHPVPLFIYDNLLRAHDKAAFVHKYIEFDLHFNRVS 87
+K ++Y++ T+ L + +++G+ Y VPL + + L A K + HK+I+ + ++S
Sbjct 502 IKEYRYESTTQTLTMEFDSGSVYEYFKVPLTVKEELDNAPSKGQYFHKFIKGKFKYEQLS 561
Score = 17.3 bits (33), Expect = 0.35
Identities = 7/16 (44%), Positives = 10/16 (63%), Gaps = 1/16 (6%)
Query 19 GAYMHKLVSVKSWKYD 34
G Y HK + K +KY+
Sbjct 544 GQYFHKFIKGK-FKYE 558
Score = 13.5 bits (23), Expect = 7.1
Identities = 5/8 (63%), Positives = 5/8 (63%), Gaps = 0/8 (0%)
Query 45 ENGTGDLY 52
E GT LY
Sbjct 78 ETGTIQLY 85
>Q8YPW9 Lysine--tRNA ligase [Nostoc sp. (strain PCC 7120 / SAG
25.82 / UTEX 2576)]
Length=561
Score = 37.4 bits (85), Expect = 3e-08
Identities = 16/60 (27%), Positives = 35/60 (58%), Gaps = 0/60 (0%)
Query 28 VKSWKYDANTRHLKIFYENGTGDLYHPVPLFIYDNLLRAHDKAAFVHKYIEFDLHFNRVS 87
+K+++Y+ T+ L + +++G+ Y VPL + + L A K + HK+I+ + ++S
Sbjct 502 IKAYRYEPTTQTLTLEFDSGSIYEYFKVPLTVKEELDNAPSKGQYFHKFIKGQFKYEQLS 561
Score = 15.8 bits (29), Expect = 1.2
Identities = 6/16 (38%), Positives = 9/16 (56%), Gaps = 1/16 (6%)
Query 19 GAYMHKLVSVKSWKYD 34
G Y HK + +KY+
Sbjct 544 GQYFHKFIK-GQFKYE 558
Score = 13.5 bits (23), Expect = 7.1
Identities = 5/8 (63%), Positives = 5/8 (63%), Gaps = 0/8 (0%)
Query 45 ENGTGDLY 52
E GT LY
Sbjct 78 ETGTIQLY 85
Lambda K H a alpha
0.332 0.144 0.457 0.792 4.96
Gapped
Lambda K H a alpha sigma
0.267 0.0410 0.140 1.90 42.6 43.6
Effective search space used: 68480
Database: e6c0932e827f2e1d8c613e2639573f8e.SwissProt.fasta
Posted date: May 20, 2024 6:36 PM
Number of letters in database: 1,122
Number of sequences in database: 2
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 11
Window for multiple hits: 40