BLASTP 2.12.0+
Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics: Alejandro A. Schaffer,
L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri
I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids
Res. 29:2994-3005.
Database: c992149ba65935a70d374cff505de920.SwissProt.fasta
15 sequences; 3,311 total letters
Query= ACIAD1548
Length=227
Score E
Sequences producing significant alignments: (Bits) Value
Q9SR36 Glutathione S-transferase U8 [Arabidopsis thaliana] 58.2 7e-14
Q9FUT0 Glutathione S-transferase U9 [Arabidopsis thaliana] 57.8 1e-13
Q9ZW24 Glutathione S-transferase U7 [Arabidopsis thaliana] 57.4 1e-13
Q9CA57 Glutathione S-transferase U10 [Arabidopsis thaliana] 56.6 3e-13
P46421 Glutathione S-transferase U5 [Arabidopsis thaliana] 52.0 1e-11
Q9ZW29 Glutathione S-transferase U2 [Arabidopsis thaliana] 51.2 2e-11
Q03662 Probable glutathione S-transferase [Nicotiana tabacum] 50.8 2e-11
P32110 Probable glutathione S-transferase [Glycine max] 50.8 3e-11
Q8L7C9 Glutathione S-transferase U20 [Arabidopsis thaliana] 48.9 1e-10
P27457 Beta-etherase [Sphingobium sp. (strain NBRC 103272 / SYK-6)] 48.9 2e-10
P30347 Protein LigF [Sphingobium sp. (strain NBRC 103272 / SYK-6)] 47.0 6e-10
P82998 Glutathione S-transferase [Pseudomonas putida (strain ATCC... 45.1 2e-09
P0ACA1 Uncharacterized GST-like protein YibF [Escherichia coli (s... 44.7 3e-09
Q9FQA3 Glutathione transferase GST 23 [Zea mays] 43.9 6e-09
P81124 Probable glutathione transferase [Aplysia californica] 41.2 1e-08
>Q9SR36 Glutathione S-transferase U8 [Arabidopsis thaliana]
Length=224
Score = 58.2 bits (139), Expect = 7e-14
Identities = 33/94 (35%), Positives = 50/94 (53%), Gaps = 5/94 (5%)
Query 12 SPFCKKVARMLQFKGIAFETVNYNGVLGAKVPRLSKVG----KVPVLDINGQRIQDSTKI 67
SPF K+V +L+ KGI +E + + V G + P L K KVPVL NG+ I +S I
Sbjct 15 SPFSKRVEMVLKLKGIPYEYIEED-VYGNRSPMLLKYNPIHKKVPVLIHNGRSIAESLVI 73
Query 68 ARYLDEAYPDLPLLYPVDPLQKAYVELWEDWSDE 101
Y+++ + + P DP ++A W + DE
Sbjct 74 VEYIEDTWKTTHTILPQDPYERAMARFWAKYVDE 107
>Q9FUT0 Glutathione S-transferase U9 [Arabidopsis thaliana]
Length=240
Score = 57.8 bits (138), Expect = 1e-13
Identities = 55/221 (25%), Positives = 106/221 (48%), Gaps = 25/221 (11%)
Query 4 VVLHQWEISPFCKKVARMLQFKGIAFETVNYNGVLGAKVPRLSKVG----KVPVLDINGQ 59
V+LH SP+ K++ L+ K I ++ V + L K L + K+PVL NG+
Sbjct 9 VILHGSFASPYSKRIELALRLKSIPYQFVQED--LQNKSQTLLRYNPVHKKIPVLVHNGK 66
Query 60 RIQDSTKIARYLDEAYPDLPLLYPVDPLQKAYVELWEDWSDELLYFYEVYFRVSDADALN 119
I +S I Y+DE + + P + P DP +++ V W ++ L+ Y++ +V ++
Sbjct 67 PISESLFIIEYIDETWSNGPHILPEDPYRRSKVRFWANYIQ--LHLYDLVIKVVKSEGEE 124
Query 120 RAVAISAEGRPKHEIVLMKPLLKAALNLQVKMQGTGR-MAKADIE-----AEFTRHLERI 173
+ A++ E + K ++ + L + + + T M+ DI + + H E +
Sbjct 125 QKKALT-EVKEKLSVIEKEGLKEIFSDTDGEPTVTNETMSLVDIVMCTLLSPYKAHEEVL 183
Query 174 -------ELVLGQTGWL--VGESKTIADIAVG-SQLLEVVR 204
E+V G GW+ + E+ + D++ Q+LE++R
Sbjct 184 GLKIIDPEIVPGVYGWINAINETSVVKDLSPPYEQILEILR 224
>Q9ZW24 Glutathione S-transferase U7 [Arabidopsis thaliana]
Length=227
Score = 57.4 bits (137), Expect = 1e-13
Identities = 60/228 (26%), Positives = 97/228 (43%), Gaps = 50/228 (22%)
Query 5 VLHQWEISPFCKKVARMLQFKGIAFETVNYNGV-LGAKVPRLSKVGK-VPVLDINGQRIQ 62
+L W SPF +++ L KG+++E + + + + +L+ V K +PVL NG+ I
Sbjct 12 LLGMWA-SPFSRRIEIALTLKGVSYEFLEQDITNKSSLLLQLNPVHKMIPVLVHNGKPIS 70
Query 63 DSTKIARYLDEAYPDLPLLYPVDPLQKAYVELWEDWSDELLYFYEVYFRVSDADALNRAV 122
+S I Y+DE + D P+L P DP ++ W + DE +Y
Sbjct 71 ESLVILEYIDETWRDNPIL-PQDPYERTMARFWSKFVDEQIY------------------ 111
Query 123 AISAEGRPKHEIVLMKPLLKAALNLQVKMQGTGRMAKADIEAEFTRHLER-IELVLGQTG 181
+ MK + K TG+ A +EA TR L +E L
Sbjct 112 -----------VTAMKVVGK-----------TGKERDAVVEA--TRDLLMFLEKELVGKD 147
Query 182 WLVGESKTIADIAVGSQLLEVVRTSKIWGPK---INSYPHIQHWIQQL 226
+L G+S DI ++RT +I G K + +P I W++ L
Sbjct 148 FLGGKSLGFVDIVATLVAFWLMRTEEIVGVKVVPVEKFPEIHRWVKNL 195
>Q9CA57 Glutathione S-transferase U10 [Arabidopsis thaliana]
Length=232
Score = 56.6 bits (135), Expect = 3e-13
Identities = 45/153 (29%), Positives = 76/153 (50%), Gaps = 10/153 (7%)
Query 2 STVVLHQWEISPFCKKVARMLQFKGIAFETVNYNGVLGAKVPRLSKVG----KVPVLDIN 57
S V+LH IS + K+V L+ KG+ +E + + L K L ++ K+PVL +
Sbjct 6 SKVILHGTWISTYSKRVEIALKLKGVLYEYLEED--LQNKSESLIQLNPVHKKIPVLVHD 63
Query 58 GQRIQDSTKIARYLDEAYPDLPLLYPVDPLQKAYVELWEDWSDELLYFYEVYFRVSDADA 117
G+ + +S I Y+DE + + P +P DP ++A V W + ++ + +EV +V +
Sbjct 64 GKPVAESLVILEYIDETWTNSPRFFPEDPYERAQVRFWVSYINQQV--FEVMGQVMSQE- 120
Query 118 LNRAVAISAEGRPKHEIVLMKPLLKAALNLQVK 150
A A S E K VL + L K N ++
Sbjct 121 -GEAQAKSVEEARKRFKVLDEGLKKHFPNKNIR 152
>P46421 Glutathione S-transferase U5 [Arabidopsis thaliana]
Length=224
Score = 52.0 bits (123), Expect = 1e-11
Identities = 35/94 (37%), Positives = 50/94 (53%), Gaps = 7/94 (7%)
Query 12 SPFCKKVARMLQFKGIAFETVNYNGVLGAKVPRLSKVG----KVPVLDINGQRIQDSTKI 67
SPF ++V L+ KGI +E V +L K P L + KVPVL NG+ I +S I
Sbjct 15 SPFSRRVEMALKLKGIPYEYVEE--ILENKSPLLLALNPIHKKVPVLVHNGKTILESHVI 72
Query 68 ARYLDEAYPDLPLLYPVDPLQKAYVELWEDWSDE 101
Y+DE +P P+L P DP +++ + DE
Sbjct 73 LEYIDETWPQNPIL-PQDPYERSKARFFAKLVDE 105
>Q9ZW29 Glutathione S-transferase U2 [Arabidopsis thaliana]
Length=225
Score = 51.2 bits (121), Expect = 2e-11
Identities = 36/104 (35%), Positives = 56/104 (54%), Gaps = 8/104 (8%)
Query 2 STVVLHQWEISPFCKKVARMLQFKGIAFETVNYNGVLGAKVPRLSKVG----KVPVLDIN 57
S +L W ISPF ++V L+ KG+ +E + + L K L ++ KVPVL N
Sbjct 7 SVKLLGFW-ISPFSRRVEMALKLKGVPYEYLEED--LPKKSTLLLELNPVHKKVPVLVHN 63
Query 58 GQRIQDSTKIARYLDEAYPDLPLLYPVDPLQKAYVELWEDWSDE 101
+ + +S I Y+D+ + + P+L P DP +KA V W + DE
Sbjct 64 DKLLSESHVILEYIDQTWNNNPIL-PHDPYEKAMVRFWAKFVDE 106
>Q03662 Probable glutathione S-transferase [Nicotiana tabacum]
Length=223
Score = 50.8 bits (120), Expect = 2e-11
Identities = 36/106 (34%), Positives = 56/106 (53%), Gaps = 9/106 (8%)
Query 1 MSTVVLHQWEISPFCKKVARMLQFKGIAFETV-----NYNGVLGAKVPRLSKVGKVPVLD 55
M+ V L + SPF ++V L+ KG+ +E + N + +L P + K KVPVL
Sbjct 1 MAEVKLLGFWYSPFSRRVEWALKIKGVKYEYIEEDRDNKSSLLLQSNP-IHK--KVPVLI 57
Query 56 INGQRIQDSTKIARYLDEAYPDLPLLYPVDPLQKAYVELWEDWSDE 101
NG+RI +S I Y+DE + + P + P DP +A W + D+
Sbjct 58 HNGKRIVESMVILEYIDETF-EGPSILPKDPYDRALARFWAKFLDD 102
Score = 16.9 bits (32), Expect = 4.4
Identities = 8/18 (44%), Positives = 10/18 (56%), Gaps = 0/18 (0%)
Query 100 DELLYFYEVYFRVSDADA 117
DELL FY + + A A
Sbjct 202 DELLAFYRSRCQAAAASA 219
>P32110 Probable glutathione S-transferase [Glycine max]
Length=225
Score = 50.8 bits (120), Expect = 3e-11
Identities = 34/94 (36%), Positives = 48/94 (51%), Gaps = 7/94 (7%)
Query 12 SPFCKKVARMLQFKGIAFETVNYNGVLGAKVPRLSKVG----KVPVLDINGQRIQDSTKI 67
SPF +V L+ KG+ ++ + N LG K L K KVPV N Q I +S I
Sbjct 16 SPFVCRVQIALKLKGVEYKFLEEN--LGNKSDLLLKYNPVHKKVPVFVHNEQPIAESLVI 73
Query 68 ARYLDEAYPDLPLLYPVDPLQKAYVELWEDWSDE 101
Y+DE + + P+L P DP Q+A W + D+
Sbjct 74 VEYIDETWKNNPIL-PSDPYQRALARFWSKFIDD 106
>Q8L7C9 Glutathione S-transferase U20 [Arabidopsis thaliana]
Length=217
Score = 48.9 bits (115), Expect = 1e-10
Identities = 34/113 (30%), Positives = 56/113 (50%), Gaps = 9/113 (8%)
Query 4 VVLHQWEISPFCKKVARMLQFKGIAFETVNYNGVLGAKVPRLSKVG----KVPVLDINGQ 59
++L W S F + L+ KG+ FE + K P L + K+PVL NG+
Sbjct 6 ILLDYWP-SMFGMRARVALREKGVEFEYREED--FSNKSPLLLQSNPIHKKIPVLVHNGK 62
Query 60 RIQDSTKIARYLDEAYPDLPLLYPVDPLQKAYVELWEDWSDELLYFYEVYFRV 112
+ +S + +Y+DEA+P+ +P DP +A W D+ D+ F + F+V
Sbjct 63 PVCESLNVVQYVDEAWPEKNPFFPSDPYGRAQARFWADFVDK--KFTDAQFKV 113
>P27457 Beta-etherase [Sphingobium sp. (strain NBRC 103272 / SYK-6)]
Length=281
Score = 48.9 bits (115), Expect = 2e-10
Identities = 31/90 (34%), Positives = 48/90 (53%), Gaps = 6/90 (7%)
Query 11 ISPFCKKVARMLQFKGIAFETV--NYNGVLGAKVPRLSKVGKVPVLDINGQRIQDSTKIA 68
ISP+ + L+ KG + V + G+L R +V PV+ +G+ + DS IA
Sbjct 20 ISPYVWRTKYALKHKGFDIDIVPGGFTGILERTGGRSERV---PVIVDDGEWVLDSWVIA 76
Query 69 RYLDEAYPDLPLLYPVDPLQKAYVELWEDW 98
YLDE YPD P+L+ P QK ++ ++W
Sbjct 77 EYLDEKYPDRPMLFE-GPTQKNLMKFLDNW 105
>P30347 Protein LigF [Sphingobium sp. (strain NBRC 103272 / SYK-6)]
Length=257
Score = 47.0 bits (110), Expect = 6e-10
Identities = 42/189 (22%), Positives = 84/189 (44%), Gaps = 11/189 (6%)
Query 44 RLSKVGKVPVLDINGQRIQDSTKIARYLDEAYPDL-PLLYPVDPLQKAYVELWEDWSDEL 102
+++ G+VP L +G+ + +ST I YL++ +P+ L P DP ++A + +W W DE
Sbjct 47 KINPRGQVPALWHDGKVVTESTVICEYLEDVFPESGNSLRPADPFKRAEMRVWTKWVDE- 105
Query 103 LYFYEVYFRVSDADALNRAVAISAEGRPKHEIVLMKPLLKAALNLQVKMQGTGRMAKADI 162
YF + A + ++ + I P+ + L + G + +
Sbjct 106 -YFCWCVSTIGWAFGIKAIAQKMSDEEFEEHINKNVPIPEQQLKWRRARNG---FPQEML 161
Query 163 EAEFTR---HLERIELVLGQTGWLVGESKTIADIAVGSQLLEVVRTSKIWGPKIN--SYP 217
+ EF + + R+E L + +LV ++ADI + + R +G +N P
Sbjct 162 DEEFRKVGVSVARLEETLSKQDYLVDTGYSLADICNFAIANGLQRPGGFFGDYVNQEKTP 221
Query 218 HIQHWIQQL 226
+ W+ ++
Sbjct 222 GLCAWLDRI 230
>P82998 Glutathione S-transferase [Pseudomonas putida (strain
ATCC 700007 / DSM 6899 / BCRC 17059 / F1)]
Length=220
Score = 45.1 bits (105), Expect = 2e-09
Identities = 32/107 (30%), Positives = 51/107 (48%), Gaps = 8/107 (7%)
Query 6 LHQWEISPFCKKVARMLQFKGIAFETVNYNGVLGAKVPRLSKVGKVPVLDINGQRIQDST 65
LH + +S + V L KG+ FE V + G + +S GKVPVL+ + +++
Sbjct 4 LHGFSVSNYYNMVKLALLEKGLPFEEVTFYGGQAPQALEVSPRGKVPVLETEHGFLSETS 63
Query 66 KIARYLDEAYPDLPLLYPVDPLQKAYVELWEDWSDELLYFYEVYFRV 112
I Y+++ LL P DP ++A V ELL E+Y +
Sbjct 64 VILDYIEQTQSGKALL-PADPFEQAKVR-------ELLKEIELYIEL 102
>P0ACA1 Uncharacterized GST-like protein YibF [Escherichia coli
(strain K12)]
Length=202
Score = 44.7 bits (104), Expect = 3e-09
Identities = 36/96 (38%), Positives = 51/96 (53%), Gaps = 7/96 (7%)
Query 12 SPFCKKVARMLQFKGIAFETVN---YNGVLGAKVPRLSKVGKVPVL-DINGQRIQDSTKI 67
SPF +K++ +L KGI FE +N YN G V + + +GKVPVL G+ DS I
Sbjct 9 SPFVRKLSILLLEKGITFEFINELPYNADNG--VAQFNPLGKVPVLVTEEGECWFDSPII 66
Query 68 ARYLDEAYPDLPLLYPVDPLQKAYVELWEDWSDELL 103
A Y+ E P + P DPL+ V E +D ++
Sbjct 67 AEYI-ELMNVAPAMLPRDPLESLRVRKIEALADGIM 101
>Q9FQA3 Glutathione transferase GST 23 [Zea mays]
Length=222
Score = 43.9 bits (102), Expect = 6e-09
Identities = 31/101 (31%), Positives = 51/101 (50%), Gaps = 7/101 (7%)
Query 5 VLHQWEISPFCKKVARMLQFKGIAFETVNYNGVLGAKVPRLSK----VGKVPVLDINGQR 60
VL W SP +V L+ KG+ +E V+ + L K L + KVPVL +G+
Sbjct 8 VLGMWA-SPMVIRVEWALRLKGVEYEYVDED--LANKSADLLRHNPVTKKVPVLVHDGKP 64
Query 61 IQDSTKIARYLDEAYPDLPLLYPVDPLQKAYVELWEDWSDE 101
+ +ST I Y+DE + + P DP ++A W ++++
Sbjct 65 VAESTIIVEYIDEVWKGGYPIMPGDPYERAQARFWARFAED 105
>P81124 Probable glutathione transferase [Aplysia californica]
Length=92
Score = 41.2 bits (95), Expect = 1e-08
Identities = 25/71 (35%), Positives = 40/71 (56%), Gaps = 5/71 (7%)
Query 13 PFCKKVARMLQFKGIAFETVNYNGVLGAKVPR---LSKVGKVPVLDINGQRIQDSTKIAR 69
P+ ++ ++ KGI+ + VN + L K L+ G+VPV+ NG + +S A
Sbjct 4 PYAQRARLIIAAKGISADLVNVD--LNKKPDHFFDLNPYGEVPVVLHNGGHVYESLIAAE 61
Query 70 YLDEAYPDLPL 80
YL+EA+PD PL
Sbjct 62 YLEEAFPDPPL 72
Lambda K H a alpha
0.323 0.138 0.420 0.792 4.96
Gapped
Lambda K H a alpha sigma
0.267 0.0410 0.140 1.90 42.6 43.6
Effective search space used: 506957
Database: c992149ba65935a70d374cff505de920.SwissProt.fasta
Posted date: May 10, 2024 4:37 AM
Number of letters in database: 3,311
Number of sequences in database: 15
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 11
Window for multiple hits: 40
Loading
Data ...
