BLASTP 2.12.0+
Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics: Alejandro A. Schaffer,
L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri
I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids
Res. 29:2994-3005.
Database: 513dda843eebac519746ed6fa1e47f7a.SwissProt.fasta
11 sequences; 6,776 total letters
Query= ACIAD1654
Length=675
Score E
Sequences producing significant alignments: (Bits) Value
P42593 2,4-dienoyl-CoA reductase [(2E)-enoyl-CoA-producing] [Esch... 835 0.0
A4J778 Metal reductase [Desulforamulus reducens (strain ATCC BAA-... 374 5e-124
P32382 NADH oxidase [Thermoanaerobacter brockii] 301 2e-96
G9F1Y9 Cinnamate reductase [Clostridium sporogenes (strain ATCC 7... 263 9e-82
P19410 3-oxocholoyl-CoA 4-desaturase [Clostridium scindens (strai... 255 5e-79
P32370 7-beta-hydroxy-3-oxochol-24-oyl-CoA 4-desaturase [Clostrid... 254 1e-78
O87278 Probable N-methylproline demethylase [Rhizobium meliloti (... 211 2e-62
P16099 Trimethylamine dehydrogenase [Methylophilus methylotrophus] 165 3e-46
P54550 NADPH dehydrogenase [Bacillus subtilis (strain 168)] 149 4e-43
A7Z6E7 NADPH dehydrogenase [Bacillus velezensis (strain DSM 23117... 147 3e-42
Q48303 Dimethylamine dehydrogenase [Hyphomicrobium sp. (strain x)] 142 2e-38
>P42593 2,4-dienoyl-CoA reductase [(2E)-enoyl-CoA-producing] [Escherichia
coli (strain K12)]
Length=672
Score = 835 bits (2157), Expect = 0.0
Identities = 420/670 (63%), Positives = 502/670 (75%), Gaps = 5/670 (1%)
Query 4 YPHLLAPLDLGFTTLKNRIMMGSMHIGLEEAPGGYERMAAFYAERAKGEVGLIVTGGIAP 63
YP L APLDLGFTTLKNR++MGSMH GLEE P G ER+AAFYAERA+ V LIV+GGIAP
Sbjct 3 YPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFYAERARHGVALIVSGGIAP 62
Query 64 NDAGRTFAHASKLDTREEAVKHKIITQAVHDAGGKIAMQILHTGRYSYQQNIVAPSAIQA 123
+ G + L+ + H+ IT+AVH GGKIA+QILHTGRYSYQ ++VAPSA+QA
Sbjct 63 DLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQILHTGRYSYQPHLVAPSALQA 122
Query 124 PINPIKPQELSSAEVWQTVSDFVNCAKLAQYAGYDGVEIMGSEGYLINEFIAARTNHRED 183
PIN P ELS E+ Q + +F CA+LA+ AGYDGVE+MGSEGYLINEF+ RTN R D
Sbjct 123 PINRFVPHELSHEEILQLIDNFARCAQLAREAGYDGVEVMGSEGYLINEFLTLRTNQRSD 182
Query 184 EWGGHYENRIRFPIEIVKRTRAEVGENFIIIYRLSMLDLVEGGSTLDEVIHLAKEIEKAG 243
+WGG Y NR+RF +E+V+ R VG +FIIIYRLSMLDLVE G T E + LA+ IE AG
Sbjct 183 QWGGDYRNRMRFAVEVVRAVRERVGNDFIIIYRLSMLDLVEDGGTFAETVELAQAIEAAG 242
Query 244 ATIINTGIGWHEARIPTIATKVPRAAFTWVTEKIRPHVKTPLITSNRINTPEMAEHVLAN 303
ATIINTGIGWHEARIPTIAT VPR AF+WVT K++ HV PL+T+NRIN P++A+ +L+
Sbjct 243 ATIINTGIGWHEARIPTIATPVPRGAFSWVTRKLKGHVSLPLVTTNRINDPQVADDILSR 302
Query 304 GQADLISMARPMLADPEFALKAGEGRSDEINTCIGCNQACLDHIFTGTVASCLVNPRACH 363
G AD++SMARP LAD E KA GR+DEINTCIGCNQACLD IF G V SCLVNPRACH
Sbjct 303 GDADMVSMARPFLADAELLSKAQSGRADEINTCIGCNQACLDQIFVGKVTSCLVNPRACH 362
Query 364 ETELKFPQADKVKQIAVIGAGPAGLSFATYAANRGHQVKIFEASEQIGGQFNIAKTIPGK 423
ET++ A + K +AV+GAGPAGL+FA AA RGHQV +F+A +IGGQFNIAK IPGK
Sbjct 363 ETKMPILPAVQKKNLAVVGAGPAGLAFAINAAARGHQVTLFDAHSEIGGQFNIAKQIPGK 422
Query 424 EEFYETLRYFKRQIELQPNIELILNHKATLDELMQASFDEIVIATGVIPRHLNIPGIDHP 483
EEFYETLRY++R IE+ + L LNH T D+L +FDE ++A+G++PR I GIDHP
Sbjct 423 EEFYETLRYYRRMIEV-TGVTLKLNHTVTADQLQ--AFDETILASGIVPRTPPIDGIDHP 479
Query 484 KVLSYLDVLKNRKPVGKRIAIIGAGGIGFDTAEFLTHEGESASLNPKKFYDEWGIDTSYA 543
KVLSYLDVL+++ PVG ++AIIG GGIGFDTA +L+ GES S N F +EWGID+S
Sbjct 480 KVLSYLDVLRDKAPVGNKVAIIGCGGIGFDTAMYLSQPGESTSQNIAGFCNEWGIDSSLQ 539
Query 544 HVGGLKQP--VIEASEREIYLLQRKASSVGANLGKTTGWIHRTGLKHRNVKMIAGASYEK 601
GGL I S R+I +LQRKAS G LGKTTGWIHRT L R VKMI G SY+K
Sbjct 540 QAGGLSPQGMQIPRSPRQIVMLQRKASKPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQK 599
Query 602 IDDQGLHIMIGDKATVLEVDNVVICAGQESYTAMYEALRESGKNVHLIGGAKEAGELDAK 661
IDD GLH++I + VL VDNVVICAGQE A+ + L +SGK VHLIGG A ELDA+
Sbjct 600 IDDDGLHVVINGETQVLAVDNVVICAGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDAR 659
Query 662 RAIRQGAELA 671
RAI QG LA
Sbjct 660 RAIAQGTRLA 669
Score = 18.9 bits (37), Expect = 8.4
Identities = 6/9 (67%), Positives = 8/9 (89%), Gaps = 0/9 (0%)
Query 2 SFYPHLLAP 10
S+ PHL+AP
Sbjct 109 SYQPHLVAP 117
>A4J778 Metal reductase [Desulforamulus reducens (strain ATCC
BAA-1160 / DSM 100696 / MI-1)]
Length=668
Score = 374 bits (961), Expect = 5e-124
Identities = 230/664 (35%), Positives = 339/664 (51%), Gaps = 37/664 (6%)
Query 7 LLAPLDLGFTTLKNRIMMGSMHIGLEEAPGGYERMAAFYAERAKGEVGLIVTGGIAPNDA 66
L +P +G L+NRI+M MH+G +++ FY RA+G GLIV GG +
Sbjct 4 LFSPAQIGTLQLRNRIIMTPMHLGYTPNGEVTDQLIEFYRVRARGGAGLIVVGGCGIDRI 63
Query 67 GRTFAHASKLDTREEAVKHKIITQAVHDAGGKIAMQILHTGRYSYQ----QNIVAPSAIQ 122
G + ++LD + + AV G KI Q+ GRY++ Q VAPS I
Sbjct 64 GNAYG-MTQLDDDRFIPGLRRLADAVQAEGAKIVAQLYQAGRYAHSALTGQPAVAPSPIP 122
Query 123 APINPIKPQELSSAEVWQTVSDFVNCAKLAQYAGYDGVEIMGSEGYLINEFIAARTNHRE 182
+ + P EL+ ++ + V+ F AK A+ AG+DGVEI+ S GYLI++F++ TN R
Sbjct 123 SKLTGETPVELTEEKIAEIVASFAKAAKRAKTAGFDGVEIIASAGYLISQFLSPLTNKRT 182
Query 183 DEWGGHYENRIRFPIEIVKRTRAEVGENFIIIYRLSMLDLVEGGSTLDEVIHLAKEIEKA 242
D +GG + R+ F +E+V R VG ++ II R++ D + G T E + +EK+
Sbjct 183 DRYGGDLQARMTFGLEVVAAVREAVGSDYPIIVRVAGNDFMPGSHTNTEAQVFCQAMEKS 242
Query 243 GATIINTGIGWHEARIPTIATKVPRAAFTWVTEKIRPHVKTPLITSNRINTPEMAEHVLA 302
G IN GWHE ++P I VP A+ ++ I+ V P+I NRINTP++AE +L
Sbjct 243 GVNAINVTGGWHETQVPQITMNVPPGAYAYLAYGIKQAVSIPVIACNRINTPDLAEAILQ 302
Query 303 NGQADLISMARPMLADPEFALKAGEGRSDEINTCIGCNQACLDHIFTGTVASCLVNPRAC 362
G+AD I MAR ++ADPE KA G ++I CIGCNQ CLDH+F SCLVN A
Sbjct 303 EGKADFIGMARSLMADPELPNKAMSGHPEQIRPCIGCNQGCLDHVFRMKPVSCLVNAEAG 362
Query 363 HETELKFPQADKVKQIAVIGAGPAGLSFATYAANRGHQVKIFEASEQIGGQFNIAKTIPG 422
E EL + +I VIGAG AGL FA AA RGH+V I+E S+Q GGQ +A PG
Sbjct 363 REAELSLTPTSQPGKILVIGAGAAGLEFARVAALRGHKVTIWEESDQAGGQLILAAAPPG 422
Query 423 KEEFYETLRYFKRQIELQPNIELILNHKATLDELMQA----SFDEIVIATGVIPRHLNIP 478
+++F Y +E+ + KAT + ++ A F+ +VIATG P IP
Sbjct 423 RKDFLHLRTYLVNACR-DLGVEIQYHTKATPENILSAVQEGKFNRVVIATGAHPITPPIP 481
Query 479 GIDHPKVLSYLDVLKNRKPVGKRIAIIGAGGIGFDTAEFLTHEGESASLNPKKFYDEWGI 538
+ KV+ DVL R G+ I I+G G +G +TA L G +
Sbjct 482 IEEGVKVIQAWDVLAGRSKAGQNIIIVGGGAVGVETALLLAESGT--------------L 527
Query 539 DTSYAHVGGLKQPVIEASEREIYLL----------QRKASSVGANLGKTTGWIHRTGLKH 588
D L+Q +E+E+Y L A+ +G ++G +T W LK
Sbjct 528 DNETLRFLMLQQ---AETEKELYRLLIQGTKKITVLEMANGIGRDIGPSTRWSMLADLKR 584
Query 589 RNVKMIAGASYEKIDDQGLHIMIGDKATVLEVDNVVICAGQESYTAMYEALRESGKNVHL 648
V + + +I +G+ + +L D V++ G S +Y+AL+ + + +
Sbjct 585 HQVNCLDETTVLEIRREGVLVKNAGTQKILPADTVILAVGSRSQNELYQALQGKVEYLSI 644
Query 649 IGGA 652
IG A
Sbjct 645 IGDA 648
>P32382 NADH oxidase [Thermoanaerobacter brockii]
Length=651
Score = 301 bits (772), Expect = 2e-96
Identities = 187/547 (34%), Positives = 302/547 (55%), Gaps = 27/547 (5%)
Query 1 MSFYPHLLAPLDLGFTTLKNRIMMGSMHIGLEEAPGGY-ERMAAFYAERAKGEVGLIVTG 59
M+ +P+L + +G ++NRI+M M L G +R+ +Y RA+G VGLI+
Sbjct 1 MTHFPNLFSEGRIGNLVIRNRIVMPPMATNLANEDGSVSQRLIDYYVARARGGVGLIILE 60
Query 60 GIAPN-DAGRTFAHASKLDTREEAVKHKIITQAVHDAGGKIAMQILHTGRYSYQQ----- 113
+ + G+ A +LD + + +AVH G KI MQI H GR +
Sbjct 61 NVQVDYPQGKNVACQLRLDDDKYMAGFFELAEAVHSYGAKIFMQIHHAGRQTTPGITEGL 120
Query 114 NIVAPSAIQAPINPIKPQELSSAEVWQTVSDFVNCAKLAQYAGYDGVEIMGSEGYLINEF 173
VAPS + +P+EL+ E+ + + FV+ A A+ A +DG+E+ G+ GYLI +F
Sbjct 121 QPVAPSPVPCSFLGTQPRELTINEIEEIIQKFVDAAVRAKGAMFDGIELHGAHGYLIGQF 180
Query 174 IAARTNHREDEWGGHYENRIRFPIEIVKRTRAEVGENFIIIYRLSMLDLVEGGSTLDEVI 233
++ RTN R D++GG +E R+RFP+EI++R + VGE++ I +R S + VEGG+TL+E
Sbjct 181 MSPRTNRRVDKYGGSFERRMRFPLEIIRRIKEAVGEDYPISFRFSADEFVEGGNTLEEGK 240
Query 234 HLAKEIEKAGATIINTGIGWHEARIPTI--ATKVPRAAFTWVTEKIRPHVKTPLITSNRI 291
+AK +E+AG +++ G +E+ +PT+ ++ + ++ E+I+ V P+IT I
Sbjct 241 QIAKMLEEAGVHVLHVSAGIYES-MPTLLEPSRFEQGWRVYLAEEIKKVVNIPVITVGVI 299
Query 292 NTPEMAEHVLANGQADLISMARPMLADPEFALKAGEGRSDEINTCIGCNQACL-DHIFTG 350
PE AE ++A G+AD +++ R ++ADPE+ KA EGR +EI CI CN C+ +F
Sbjct 300 REPEFAEKIIAEGRADFVAVGRGLIADPEWPKKAKEGRQNEIRKCISCNIGCIGGRVFQN 359
Query 351 TVASCLVNPRACHE---TELKFPQADKVKQIAVIGAGPAGLSFATYAANRGHQVKIFEAS 407
C VNP A E +E+K QA K++ V+G GPAG+ A AA RGHQV ++E
Sbjct 360 LRLRCTVNPVAGREGVYSEIK--QAPVKKKVVVVGGGPAGMQAAITAAKRGHQVILYEKK 417
Query 408 EQIGGQFNIAKTIPGKEEFYETLRYFKRQIELQ---PNIELILNHKATLDELMQASFDEI 464
+ +GGQ IA PGK + +++F+ +E + +E+ A + + S D +
Sbjct 418 QHLGGQLEIASASPGKAK----IKWFRDWLEAELSRAGVEVRSGVTADAETIAALSPDYV 473
Query 465 VIATGVIPRHLNIPGIDHPK--VLSYLDVLKNRKPVGK--RIAIIGAGGIGFDTAEFLTH 520
++ATG P I G + V DVL + K + +IG G +G +TA +L
Sbjct 474 ILATGSEPVTPRIKGAEKENTFVFQAWDVLAGKVSFDKDEEVVVIGGGLVGCETAHYLAE 533
Query 521 EGESASL 527
+G ++
Sbjct 534 KGAKVTI 540
Score = 18.9 bits (37), Expect = 8.4
Identities = 9/38 (24%), Positives = 21/38 (55%), Gaps = 3/38 (8%)
Query 616 TVLEVDNVV---ICAGQESYTAMYEALRESGKNVHLIG 650
T+ E++ ++ + A + AM++ + G + +LIG
Sbjct 141 TINEIEEIIQKFVDAAVRAKGAMFDGIELHGAHGYLIG 178
>G9F1Y9 Cinnamate reductase [Clostridium sporogenes (strain ATCC
7955 / DSM 767 / NBRC 16411 / NCIMB 8053 / NCTC 8594 / PA
3679)]
Length=665
Score = 263 bits (672), Expect = 9e-82
Identities = 198/682 (29%), Positives = 323/682 (47%), Gaps = 70/682 (10%)
Query 4 YPHLLAPLDLGFTTLKNRIMMGSMHIG-LEEAPGGY-ERMAAFYAERAKGEVGLIVTGGI 61
Y L P+ +G +KNR ++ M G + A G + +R FY ERAKG GLI+TG
Sbjct 5 YKVLYDPIKIGKLEIKNRYVLAPMGPGGMCNADGSFNKRGIEFYVERAKGGTGLIMTGVT 64
Query 62 APNDAGRTFAHASK----LDTREEAVKHKIITQAVHDAGGKIAMQI-LHTGRYSYQQNI- 115
+ A S ++ +T+ VH G KI +Q+ GR S +
Sbjct 65 MVENNIEKCALPSMPCPTINPLNFITTGNEMTERVHAYGAKIFLQLSAGFGRVSIPSIVG 124
Query 116 ---VAPSAIQAPINP-IKPQELSSAEVWQTVSDFVNCAKLAQYAGYDGVEIMG-SEGYLI 170
VAPS I P + +EL++ EV + V F A++A+ AG+DGVEI EGYL+
Sbjct 125 KVAVAPSKIPHRFLPGVTCRELTTEEVKEYVKAFGESAEIAKKAGFDGVEIHAVHEGYLL 184
Query 171 NEFIAARTNHREDEWGGHYENRIRFPIEIVKRTRAEVGENFIIIYRLSMLDLV------- 223
++F + NHR DE+GG ENR+RF E+V+ + G++F + R S+ +
Sbjct 185 DQFAISFFNHRTDEYGGSLENRLRFACEVVQEIKKRCGQDFPVSLRYSIKSFIKDWCKGG 244
Query 224 -------EGGSTLDEVIHLAKEIEKAGATIINTGIG----WHEARIPTIATKVPRAAFTW 272
E G + E I AK + AG +N +G W+ + P K +
Sbjct 245 LPDEEFEEKGRDIPEGIEAAKILVAAGYDALNGDVGSYDSWYWSHPPMYQKKGLYLPYNE 304
Query 273 VTEKIRPHVKTPLITSNRINTPEMAEHVLANGQADLISMARPMLADPEFALKAGEGRSDE 332
+ +K+ V P+IT+ R+ PE++ + +G+ D+I++ RP+LAD E K E + D+
Sbjct 305 ILKKV---VDVPIITAGRMEDPELSSDAILSGKTDMIALGRPLLADAEIPNKIFEDKYDK 361
Query 333 INTCIGCNQACLDHIFTGTVASCLVNPRACHETELKFPQADKVKQIAVIGAGPAGLSFAT 392
+ C+ C + C+ + SC VNP E E +A+++K++ ++G G AG+ A
Sbjct 362 VRPCLSCQEGCMGRLQNFATVSCAVNPACGREKEYGLKKAEQIKKVLIVGGGVAGMEAAR 421
Query 393 YAANRGHQVKIFEASEQIGGQFNIAKTIPGKEEFYETLRYFKRQIELQPNIELILNHKAT 452
AA RGH+V + E + +GG + +P ++ L + I +E+ LN A+
Sbjct 422 VAAIRGHKVTLIEKNGYLGGNI-VPGGVPDFKDDDRALVKWYEGILKDLGVEIKLNVAAS 480
Query 453 LDELMQASFDEIVIATGVIPRHLNIPGIDHPKVLSYLDVLKNRKPVGKRIAIIGAGGIGF 512
+ + + DE+++ATG PR L I G D KV S DVL RK VG+++ IIG G +G
Sbjct 481 KENIKEFGADEVLLATGSSPRTLTIEGAD--KVYSAEDVLMERKNVGEKVIIIGGGLVGC 538
Query 513 DTAEFLTHEGESASLNPKKFYDEWGIDTSYAHVGGLKQPVIEASEREIYLLQRKASSVGA 572
+TA +L +G+ ++ + VGG P+ A+ + L
Sbjct 539 ETALWLKQQGKEVTIVE--------MQNDILQVGG---PLCHANHDMLIDL--------- 578
Query 573 NLGKTTGWIHRTGLKHRNVKMIAGASYEKIDDQGLHIMIGDKATVLEVDNVVICAGQESY 632
+K + + A + K D+G + + +++ D+ V+ G S
Sbjct 579 -------------IKFNKIDVKASSYISKKTDEGFVLNTNGEESIINADSAVVAIGYLSE 625
Query 633 TAMYEALRESGKNVHLIGGAKE 654
+Y +R N LIG A +
Sbjct 626 KDLYSEVRFDIPNARLIGDANK 647
>P19410 3-oxocholoyl-CoA 4-desaturase [Clostridium scindens (strain
JCM 10418 / VPI 12708)]
Length=639
Score = 255 bits (651), Expect = 5e-79
Identities = 194/680 (29%), Positives = 311/680 (46%), Gaps = 66/680 (10%)
Query 4 YPHLLAPLDLGFTTLKNRIMMGSMHIGLEEAPGGY-ERMAAFYAERAKGEVGLIVTGGIA 62
Y L +P + LKNRI++ M+ + + E M A++ RAK L + +A
Sbjct 3 YEALFSPFKVRGLELKNRIVLPGMNTKMAKNKHDIGEDMIAYHVARAKAGCALNIFECVA 62
Query 63 PNDAGRTFAHASKLDTREEAVKHKIITQAVHDAGGKIAMQILHTGRYSYQQNIVAPSAIQ 122
A + + L T + K +T AVH+AGGK+ +Q+ H G +S Q + ++
Sbjct 63 LCPAPHAYMYMG-LYTDHHVEQLKKLTDAVHEAGGKMGIQLWHGG-FSPQMFFDETNTLE 120
Query 123 APINPIKPQELSSAEVWQTVSDFVNCAKLAQYAGYDGVEIMGSEGYLINEFIAARTNHRE 182
P L+ + + V +F A++A AG+D VE + YL +EF++ N R
Sbjct 121 TP------DTLTVERIHEIVEEFGRGARMAVQAGFDAVEFHAAHSYLPHEFLSPGMNKRT 174
Query 183 DEWGGHYENRIRFPIEIVKRTRAEVGENFIIIYRLSMLD-LVEGGSTLDEVIHLAKEIEK 241
DE+GG +ENR RF E+V+ R+ + ++ R +D L+E T +E++ + +
Sbjct 175 DEYGGSFENRCRFCYEVVQAIRSNIPDDMPFFMRADCIDELMEQTMTEEEIVTFINKCAE 234
Query 242 AGATIINTGIGWHEARIPTIATKVP-RAAFTWVTEKI---RPHVKTPLITSNRINTPEMA 297
G + + G + T+ P A + E I + + P++ RINT EMA
Sbjct 235 LGVDVADLSRG-NATSFATVYEVPPFNLAHGFNIENIYNIKKQINIPVMGVGRINTGEMA 293
Query 298 EHVLANGQADLISMARPMLADPEFALKAGEGRSDEINTCIGCNQACLDHIFTGTV--ASC 355
V+ G+ DL+ + R LADP + K EG+ D I CIGC+Q C D + + +C
Sbjct 294 NKVIEEGKFDLVGIGRAQLADPNWITKVREGKEDLIRHCIGCDQGCYDAVINPKMKHITC 353
Query 356 LVNPRACHETELKFPQADKVKQIAVIGAGPAGLSFATYAANRGHQVKIFEASEQIGGQFN 415
NP C E + P+ D K++ ++G G AG+ A RGH IFEAS+++ GQF
Sbjct 354 THNPGLCLEYQ-GMPKTDAPKKVMIVGGGMAGMIAAEVLKTRGHNPVIFEASDKLAGQFR 412
Query 416 IAKTIPGKEEFYETLRYFKRQIELQPNIELILNHKATLDELMQASFDEIVIATGVIPRHL 475
+A P K+++ + + +++E + IE+ LN + T + + + + D ++IA G
Sbjct 413 LAGVAPMKQDWADVAEWEAKEVE-RLGIEVRLNTEVTAETIKEFNPDNVIIAVGSTYALP 471
Query 476 NIPGIDHPKVLSYLDVLKNRKPVGKRIAIIGAGGIGFDTAEFLTHEGESASLNPKKFYDE 535
IPGID P V S VLK R+A+IG G +G + AE L G
Sbjct 472 EIPGIDSPSVYSQYQVLKGEVNPTGRVAVIGCGLVGTEVAELLASRG------------- 518
Query 536 WGIDTSYAHVGGLKQPVIEASEREIYLLQRKASSVGANLGKTTGWIHRTGLKHRNVKMIA 595
++ ++RK VG L K+ + ++
Sbjct 519 ----------------------AQVIAIERK--GVGTGLSMLRRMFMNPEFKYYKIAKMS 554
Query 596 GASYEKIDDQGLHIMIGDKAT------VLEVDNVVICAG--QESYTAMYEALRESGKNVH 647
G + ++ +H ++ DK T VLE D VIC G + E G V
Sbjct 555 GTNVTALEQGKVHYIMTDKKTKEVTQGVLECDATVICTGITARPSDGLKARCEELGIPVE 614
Query 648 LIGGAKEAGELDAKRAIRQG 667
+IG A AG D A R+G
Sbjct 615 VIGDA--AGARDCTIATREG 632
>P32370 7-beta-hydroxy-3-oxochol-24-oyl-CoA 4-desaturase [Clostridium
scindens (strain JCM 10418 / VPI 12708)]
Length=661
Score = 254 bits (650), Expect = 1e-78
Identities = 189/682 (28%), Positives = 317/682 (46%), Gaps = 36/682 (5%)
Query 4 YPHLLAPLDLGFTTLKNRIMMGSMHIGLEEAPGGY-ERMAAFYAERAKGEVGLIVTGGIA 62
+ L +PL +G TL NR+ M M + E A G +R+A + RA+G G ++ +
Sbjct 5 HSRLFSPLQIGSLTLSNRVGMAPMSMDYEAADGTVPKRLADVFVRRAEGGTGYVMIDAVT 64
Query 63 PNDAGRTFAHASKLDTREEAVKHKIITQAVHDAGGKIAMQILHTGRYS---YQQNIVAPS 119
+ + + LD E + K V +AG + QI+H G S Y+
Sbjct 65 IDSKYPYMGNTTALDRDELVPQFKEFADRVKEAGSTLVPQIIHPGPESVCGYRHIAPLGP 124
Query 120 AIQAPINPIKPQELSSAEVWQTVSDFVNCAKLAQYAGYDGVEIMGSEGYLI-NEFIAART 178
+ N + +S E+ + F A+ A+ AG + + + Y++ F++
Sbjct 125 SANTNANCHVSRSISIDEIHDIIKQFGQAARRAEEAGCGAISLHCAHAYMLPGSFLSPLR 184
Query 179 NHREDEWGGHYENRIRFPIEIVKRTRAEVGENFIIIYRLSMLDLVEGGSTLDEVIHLAKE 238
N R DE+GG +NR RF IE+++ R V +F I R+S + + GG++L+++++LA +
Sbjct 185 NKRMDEYGGSLDNRARFVIEMIEEARRNVSPDFPIFLRISGDERMVGGNSLEDMLYLAPK 244
Query 239 IEKAGATIINTGIGWHEARIPTI--ATKVPRAAFTWVTEKIRPHVKTPLITSNRINTPEM 296
E AG +++ G + I R + +I+ V P+ +IN
Sbjct 245 FEAAGVSMLEVSGGTQYEGLEHIIPCQNKSRGVNVYEASEIKKVVGIPVYAVGKINDIRY 304
Query 297 AEHVLANGQADLISMARPMLADPEFALKAGEGRSDEINTCIGCNQACLDHIFTGTVASCL 356
A ++ G D ++M RP+LADP+ KA EG+ DEI C C +C+ C
Sbjct 305 AAEIVERGLVDGVAMGRPLLADPDLCKKAVEGQFDEITPCASCGGSCISRSEAAPECHCH 364
Query 357 VNPRACHETELKFPQADKVKQIAVIGAGPAGLSFATYAANRGHQVKIFEASEQIGGQFNI 416
+NPR E E A+K K++ VIGAGP G+ A AA RGH V ++EA ++IGGQ N+
Sbjct 365 INPRLGREYEFPDVPAEKSKKVLVIGAGPGGMMAAVTAAERGHDVTVWEADDKIGGQLNL 424
Query 417 AKTIPGKEEFYETLRYFKRQIELQPNIELILNHKATLDELMQASFDEIVIATGVIPRHLN 476
A PGK+E + + + + + + ++ N +AT +++ + + +++ATG P
Sbjct 425 AVVAPGKQEMTQWMVHLNYRAK-KAGVKFEFNKEATAEDVKALAPEAVIVATGAKPLVPP 483
Query 477 IPGIDHPKVLSYLDVLKNRKPVGK-RIAIIGAGGIGFDTAEFLTHEGESASLNPKKFYDE 535
I G VL+ D L+ + + K R+ ++G G + +TAE S + YD
Sbjct 484 IKGTQDYPVLTAHDFLRGKFVIPKGRVCVLGGGAVACETAETALENARPNSYT--RGYDA 541
Query 536 WGIDTSYAHVGGLKQPV--IEASEREIYLLQRKASSVGANLGKTTGWIHRTGLKHRNVKM 593
D V L Q + + A RE + + K+ V N+ N K+
Sbjct 542 SIGDIDVTLVEMLPQLLTGVCAPNREPLIRKLKSKGVHINV---------------NTKI 586
Query 594 IAGASYE-KIDDQGLHIMIGDKATVLEVDNVVICAGQESYTAMYEALRESGKNVHLIGGA 652
+ +E K+ Q G + + D V+ G +Y + E L+E VH+IG A
Sbjct 587 MEVTDHEVKVQRQD-----GTQEWLEGFDYVLFGLGSRNYDPLSETLKEFVPEVHVIGDA 641
Query 653 KEAGELDAKRAIRQGAELAATL 674
A + A A+ +G E A +L
Sbjct 642 VRARQ--ASYAMWEGFEKAYSL 661
>O87278 Probable N-methylproline demethylase [Rhizobium meliloti
(strain 1021)]
Length=678
Score = 211 bits (536), Expect = 2e-62
Identities = 151/531 (28%), Positives = 249/531 (47%), Gaps = 19/531 (4%)
Query 7 LLAPLDLGFTTLKNRIMMGSMHIGLEEAPGGYERMAAFYAERAKGEVGLIVTGGIAPNDA 66
LL P L TL+NRI++ + E ER A+ ERA+G V + +T G A
Sbjct 6 LLQPYQLKHLTLRNRIIVTAHEPAYPEDGMPKERYRAYTVERARGGVAMTMTAGSAAVSK 65
Query 67 GRTFAHASKLDTREEAVKH-KIITQAVHDAGGKIAMQILHTGRYSYQQN-----IVAPSA 120
+ L R+E V + +T AVH+ G I +Q+ H GR + +VAPS
Sbjct 66 DSPPVFNNLLAYRDEIVPWIREMTDAVHEEGAVIMIQLTHLGRRTRWDKGDWLPVVAPSH 125
Query 121 IQAPINPIKPQELSSAEVWQTVSDFVNCAKLAQYAGYDGVEIMGSEGYLINEFIAARTNH 180
+ + P+++ ++ + + DF + A+ + G DGVE+ + G+LI++F + TN
Sbjct 126 HREAAHRAFPKKIEDWDIDRIIKDFADAAERMKAGGMDGVELE-AYGHLIDQFASPLTNE 184
Query 181 REDEWGGHYENRIRFPIEIVKRTRAEVGENFIIIYRLSMLDLVEGGSTLDEVIHLAKEIE 240
+ +GG +NR+RF +++K RA VG+ FI+ R + + + GG+ E + ++K ++
Sbjct 185 LDGPYGGSLDNRMRFCFDVLKAIRARVGDEFILGVRYTADECLPGGTDKAEGLEISKRLK 244
Query 241 KAGATIINTGIGWHEARIPTIATKVP-----RAAFTWVTEKIRPHVKTPLITSNRINTPE 295
++G I H P + +P + +IR P + +I
Sbjct 245 ESGLIDYLNIIRGHIDTDPGLTDVIPIQGMANSPHLDFAGEIRAATNFPTFHAAKIPDVA 304
Query 296 MAEHVLANGQADLISMARPMLADPEFALKAGEGRSDEINTCIGCNQACLDHIFTGTVASC 355
A H +A+G+ D++ M R + DP K E R ++I C+G N CLD I+ G A C
Sbjct 305 TARHAIASGKVDMVGMTRAHMTDPHIVRKIIEKREEDIRPCVGANY-CLDRIYQGGAAYC 363
Query 356 LVNPRACHETEL--KFPQADKVKQIAVIGAGPAGLSFATYAANRGHQVKIFEASEQIGGQ 413
+ N E + +A +++ V+G GPAGL A A RGH+V +FEA+ GGQ
Sbjct 364 IHNAATGRELTMPHSIAKAHCRRKVVVVGTGPAGLEAARVAGERGHEVIVFEAASDPGGQ 423
Query 414 FNIAKTIPGKEEFYETLRYFKRQIELQPNIELILNHKATLDELMQASFDEIVIATGVIPR 473
+ P + E + + Q E + + N A + + S D ++IATG +P
Sbjct 424 VRLTAQSPRRREMISIIDWRMSQCE-KLGVTFHFNTWAEAEAIQAESPDVVIIATGGLP- 481
Query 474 HLNIPGIDHPKVLSYLDVLKNRKPVGKRIAIIGAGG--IGFDTAEFLTHEG 522
H + + V+S D++ G + I G AEFL G
Sbjct 482 HTEVLSRGNELVVSAWDIISGDAKPGTNVLIFDDAGDHAALQAAEFLATAG 532
Score = 23.1 bits (48), Expect = 0.42
Identities = 22/93 (24%), Positives = 38/93 (41%), Gaps = 7/93 (8%)
Query 386 AGLSFATYAANRGHQVKIFEASEQIGGQFNIAKTIP-----GKEEFYETLRYFKRQIELQ 440
A L A + A G +V+I + +P K + T+ Y +E
Sbjct 520 AALQAAEFLATAGARVEIMTPDRSFAPEVMAMNLVPYMRCLQKLDVTFTVTYRLEAVEKS 579
Query 441 PNIELILNHKATLDELM-QASFDEIVIATGVIP 472
N EL+ + + + Q +FD++V+ G IP
Sbjct 580 GN-ELVAHVGSDYGGISKQRTFDQVVVNHGTIP 611
>P16099 Trimethylamine dehydrogenase [Methylophilus methylotrophus]
Length=730
Score = 165 bits (418), Expect = 3e-46
Identities = 145/533 (27%), Positives = 239/533 (45%), Gaps = 37/533 (7%)
Query 7 LLAPLDLGFTTLKNRIMMGSMHIGLEEAPGGYERMAAFYAERAKGEVGLIVTGGIAPN-D 65
L P+ +G TL+NR IG G++ +A + +A+G + T + N +
Sbjct 10 LFEPIQIGPKTLRNRFYQVPHCIGAGSDKPGFQ--SAHRSVKAEGGWAALNTEYCSINPE 67
Query 66 AGRTFAHASKLDTREEAVKHKIITQAVHDAGGKIAMQILHTGRYSYQQNIVA----PSAI 121
+ T ++++ + K +T VH G +++ + G ++ A PS
Sbjct 68 SDDTHRLSARIWDEGDVRNLKAMTDEVHKYGALAGVELWYGGAHAPNMESRATPRGPSQY 127
Query 122 QAPINPIKP-QELSSAEVWQTVSDFVNCAKLAQYAGYDGVEIMGSEGYLINEFIAARTNH 180
+ + +E+ +++ Q +V+ AK ++ AG+D V + G+ YL +F+ N
Sbjct 128 ASEFETLSYCKEMDLSDIAQVQQFYVDAAKRSRDAGFDIVYVYGAHSYLPLQFLNPYYNK 187
Query 181 REDEWGGHYENRIRFPIEIVKRTRAEVGENFIIIYRLSMLDLVEGGSTLDEVIHLAKEIE 240
R D++GG ENR RF +E +++ + VG + I R + D V G ++ + K +E
Sbjct 188 RTDKYGGSLENRARFWLETLEKVKHAVGSDCAIATRFGV-DTVYGPGQIEAEVDGQKFVE 246
Query 241 KAGATI------INTGIGWHEARIPTIATKVPRAAFTWVTEKIRPHVKTPLITSNRINTP 294
A + + I W E P+ + WV + ++ K P++ R P
Sbjct 247 MADSLVDMWDITIGDIAEWGEDAGPSRFYQQGHT-IPWV-KLVKQVSKKPVLGVGRYTDP 304
Query 295 EMAEHVLANGQADLISMARPMLADPEFALKAGEGRSDEINTCIGCNQACLDHIFTGTVAS 354
E ++ G AD+I ARP +ADP K +GR D+I CIGCN G
Sbjct 305 EKMIEIVTKGYADIIGCARPSIADPFLPQKVEQGRYDDIRVCIGCNVCISRWEIGGPPMI 364
Query 355 CLVNPRACHETEL-----KFPQADKVKQIAVIGAGPAGLSFATYAANRGHQVKIFEASEQ 409
C N A E KF Q + ++GAGP+G A G+ V + + +E+
Sbjct 365 CTQNATAGEEYRRGWHPEKFRQTKNKDSVLIVGAGPSGSEAARVLMESGYTVHLTDTAEK 424
Query 410 IGGQFNIAKTIPGKEEFYETLRYFKRQIE--LQPNIE--LILNHK-ATLDELMQASFDEI 464
IGG N +PG E+ Y + QI L+ N E L L K T D+++Q D++
Sbjct 425 IGGHLNQVAALPGLGEWSYHRDYRETQITKLLKKNKESQLALGQKPMTADDVLQYGADKV 484
Query 465 VIATGV--------IPRHLNIPGIDH--PKVLSYLDVLKNRKPVGKRIAIIGA 507
+IATG H IPG D P L+ V+ +K +GKR+ I+ A
Sbjct 485 IIATGARWNTDGTNCLTHDPIPGADASLPDQLTPEQVMDGKKKIGKRVVILNA 537
Score = 23.5 bits (49), Expect = 0.32
Identities = 13/36 (36%), Positives = 18/36 (50%), Gaps = 0/36 (0%)
Query 621 DNVVICAGQESYTAMYEALRESGKNVHLIGGAKEAG 656
D+V+I S + L ESG VHL A++ G
Sbjct 391 DSVLIVGAGPSGSEAARVLMESGYTVHLTDTAEKIG 426
>P54550 NADPH dehydrogenase [Bacillus subtilis (strain 168)]
Length=338
Score = 149 bits (376), Expect = 4e-43
Identities = 111/330 (34%), Positives = 165/330 (50%), Gaps = 9/330 (3%)
Query 7 LLAPLDLGFTTLKNRIMMGSMHIGLEEAPGGYERM--AAFYAERAKGEVGLIVTGGIAPN 64
L P+ + TLKNRI+M M + G A Y RA G+VGLI+ A N
Sbjct 5 LFTPITIKDMTLKNRIVMSPMCMYSSHEKDGKLTPFHMAHYISRAIGQVGLIIVEASAVN 64
Query 65 DAGRTFAHASKLDTREEAVKHKIITQAVHDAGGKIAMQILHTGRYS-YQQNIVAPSAIQA 123
GR + + E +T+ V + G KI +Q+ H GR + + +I APSAI
Sbjct 65 PQGRITDQDLGIWSDEHIEGFAKLTEQVKEQGSKIGIQLAHAGRKAELEGDIFAPSAIAF 124
Query 124 PINPIKPQELSSAEVWQTVSDFVNCAKLAQYAGYDGVEIMGSEGYLINEFIAARTNHRED 183
P E+S+ +V +TV +F A A+ AG+D +EI + GYLI+EF++ +NHR D
Sbjct 125 DEQSATPVEMSAEKVKETVQEFKQAAARAKEAGFDVIEIHAAHGYLIHEFLSPLSNHRTD 184
Query 184 EWGGHYENRIRFPIEIVKRTRAEVGENFIIIYRLSMLDLVEGGSTLDEVIHLAKEIEKAG 243
E+GG ENR RF EI+ + +V + + + R+S D + G + + I AK +++ G
Sbjct 185 EYGGSPENRYRFLREIIDEVK-QVWDGPLFV-RVSASDYTDKGLDIADHIGFAKWMKEQG 242
Query 244 ATIINTGIGWHEARIPTIATKVPRAAFTWVTEKIRPHVKTPLITSNRINTPEMAEHVLAN 303
+I+ G A + P ++ EKIR I MAE +L N
Sbjct 243 VDLIDCSSG---ALVHADINVFPGYQVSF-AEKIREQADMATGAVGMITDGSMAEEILQN 298
Query 304 GQADLISMARPMLADPEFALKAGEGRSDEI 333
G+ADLI + R +L DP FA A + + EI
Sbjct 299 GRADLIFIGRELLRDPFFARTAAKQLNTEI 328
>A7Z6E7 NADPH dehydrogenase [Bacillus velezensis (strain DSM 23117
/ BGSC 10A6 / LMG 26770 / FZB42)]
Length=338
Score = 147 bits (370), Expect = 3e-42
Identities = 107/339 (32%), Positives = 173/339 (51%), Gaps = 9/339 (3%)
Query 7 LLAPLDLGFTTLKNRIMMGSMHI-GLEEAPGGYERM-AAFYAERAKGEVGLIVTGGIAPN 64
L P + T+KNRI+M M + E G + A Y RA G+VGLI+ A N
Sbjct 5 LFTPWTVKDVTIKNRIVMAPMCMYSSHEKDGKLQPFHMAHYISRAIGQVGLIIVEATAVN 64
Query 65 DAGRTFAHASKLDTREEAVKHKIITQAVHDAGGKIAMQILHTGRYS-YQQNIVAPSAIQA 123
GR + + + +T+ V G KI +Q+ H GR + + +I APSAI
Sbjct 65 PQGRISDQDLGIWSDDHIEGFAKLTEQVKAQGSKIGIQLAHAGRKAELEGDIYAPSAIPF 124
Query 124 PINPIKPQELSSAEVWQTVSDFVNCAKLAQYAGYDGVEIMGSEGYLINEFIAARTNHRED 183
P E+++ ++ +T+ +F A A+ AG+D +E+ + GYL++EF++ +NHR D
Sbjct 125 DEQSKTPAEMTTEQIKETIQEFKQAAARAKEAGFDIIELHAAHGYLMHEFLSPLSNHRTD 184
Query 184 EWGGHYENRIRFPIEIVKRTRAEVGENFIIIYRLSMLDLVEGGSTLDEVIHLAKEIEKAG 243
E+GG +ENR RF E ++ + EV + + + R+S D + G + + I AK +++ G
Sbjct 185 EYGGSHENRYRFLGETIEAVK-EVWDGPLFV-RISASDYTDKGLDIADHIGFAKWMKEQG 242
Query 244 ATIINTGIGWHEARIPTIATKVPRAAFTWVTEKIRPHVKTPLITSNRINTPEMAEHVLAN 303
+I+ G A + P ++ EKIR + I T MAE +L N
Sbjct 243 VDLIDCSSG---ALVQADINVFPGYQVSF-AEKIREQAEIATGAVGLITTGTMAEEILQN 298
Query 304 GQADLISMARPMLADPEFALKAGEGRSDEINTCIGCNQA 342
+ADLI +AR +L DP FA A + + EI + + ++A
Sbjct 299 NRADLIFVARELLRDPHFARSAAKQLNTEIPSPVQYDRA 337
>Q48303 Dimethylamine dehydrogenase [Hyphomicrobium sp. (strain
x)]
Length=736
Score = 142 bits (357), Expect = 2e-38
Identities = 116/395 (29%), Positives = 176/395 (45%), Gaps = 29/395 (7%)
Query 137 EVWQTVSDFVNCAKLAQYAGYDGVEIMGSEGYLINEFIAARTNHREDEWGGHYENRIRFP 196
E+ + +V+ A A+ G+D V + G+ Y +++ N R D++GG ++NR RF
Sbjct 150 ELERLQQQYVDAALRARDTGFDLVNVYGAHAYGPMQWLNPYYNRRTDKYGGSFDNRARFW 209
Query 197 IEIVKRTRAEVGENFIIIYRLSMLDLVEGGSTLDEVIHLAKEIEKAGATI----INTG-- 250
IE +++ R V ++ ++ R + D + G ++ + IE A + +N G
Sbjct 210 IETLEKIRRAVNDDVALVTRCAT-DTLYGTKGVELTEDGLRFIELASPYLDLWDVNIGDI 268
Query 251 IGWHEARIPTIATKVPRAAFTWVTEKIRPHVKTPLITSNRINTPEMAEHVLANGQADLIS 310
W E P+ + W+ I+ P++ R PE V+ G D+I
Sbjct 269 AEWGEDAGPSRFYPIAHEN-DWIRH-IKQATNKPVVGVGRYYDPEKMLQVIKAGIIDIIG 326
Query 311 MARPMLADPEFALKAGEGRSDEINTCIGCNQACLDHIFTGTVASCLVNPRACHETEL--- 367
ARP +ADP K EGR D+I TCIGCN G C N A E
Sbjct 327 AARPSIADPWLPRKIDEGRVDDIRTCIGCNVCISRWEMGGVPFICTQNATAGEEYRRGWH 386
Query 368 --KFPQADKVKQIAVIGAGPAGLSFATYAANRGHQVKIFEASEQIGGQFNIAKTIPGKEE 425
KF + ++GAGPAG A RG+ V + + E+ GG N T+PG E
Sbjct 387 PEKFEPKKSDHDVLIVGAGPAGSECARVLMERGYTVHLVDTREKTGGYVNDVATLPGLGE 446
Query 426 FYETLRYFKRQIE----LQPNIELILNHK-ATLDELMQASFDEIVIATGV--------IP 472
+ Y + Q+E P ++ L K T D+++Q +VIATG
Sbjct 447 WSFHRDYRQTQLEKLLKKNPECQIALKQKPMTADDILQYGASRVVIATGAKWSTTGVNHR 506
Query 473 RHLNIPGIDH--PKVLSYLDVLKNRKPVGKRIAII 505
H IPG D P VL+ V + +K VGKR+ II
Sbjct 507 THEPIPGADASLPHVLTPEQVYEGKKAVGKRVMII 541
Lambda K H a alpha
0.319 0.136 0.398 0.792 4.96
Gapped
Lambda K H a alpha sigma
0.267 0.0410 0.140 1.90 42.6 43.6
Effective search space used: 3826020
Database: 513dda843eebac519746ed6fa1e47f7a.SwissProt.fasta
Posted date: May 13, 2024 9:02 AM
Number of letters in database: 6,776
Number of sequences in database: 11
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 11
Window for multiple hits: 40
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Data ...
