BLASTP 2.12.0+
Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics: Alejandro A. Schaffer,
L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri
I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids
Res. 29:2994-3005.
Database: d95dce22a6b07c9e4317008fa7aa2158.SwissProt.fasta
12 sequences; 7,412 total letters
Query= ACIAD1724
Length=625
Score E
Sequences producing significant alignments: (Bits) Value
Q9S725 4-coumarate--CoA ligase 2 [Arabidopsis thaliana] 78.6 2e-18
A1L1K7 Long-chain-fatty-acid--CoA ligase ACSBG2 [Rattus norvegicus] 74.3 5e-17
P08659 Luciferin 4-monooxygenase [Photinus pyralis] 72.4 2e-16
P14913 4-coumarate--CoA ligase 2 [Petroselinum crispum] 71.2 4e-16
Q96GR2 Long-chain-fatty-acid--CoA ligase ACSBG1 [Homo sapiens] 70.9 7e-16
Q5FVE4 Long-chain-fatty-acid--CoA ligase ACSBG2 [Homo sapiens] 70.1 1e-15
Q9S777 4-coumarate--CoA ligase 3 [Arabidopsis thaliana] 69.7 1e-15
M4IS90 Probable CoA ligase CCL8 [Humulus lupulus] 69.7 1e-15
Q4R4P9 Long-chain-fatty-acid--CoA ligase ACSBG1 [Macaca fascicula... 69.7 2e-15
P14912 4-coumarate--CoA ligase 1 [Petroselinum crispum] 69.3 2e-15
A0A2H5AIX5 4-coumarate-CoA ligase 1 [Narcissus pseudonarcissus] 68.9 2e-15
Q9LK39 Probable acyl-activating enzyme 16, chloroplastic [Arabido... 67.8 6e-15
>Q9S725 4-coumarate--CoA ligase 2 [Arabidopsis thaliana]
Length=556
Score = 78.6 bits (192), Expect = 2e-18
Identities = 106/413 (26%), Positives = 170/413 (41%), Gaps = 63/413 (15%)
Query 74 SYAETLQRAWHIAQALHQRNLSQERPLVILSGNDLEHLTLSMGAMLAGVPFSAISPAYSL 133
+YA+ + +A LH + Q ++IL N E + + A G ++ +P +
Sbjct 65 TYADVHVTSRKLAAGLHNLGVKQHDVVMILLPNSPEVVLTFLAASFIGAITTSANPFF-- 122
Query 134 VSQDFGKLKHVFDVLTPGMVYANDGQAFAKAI--QSCTQDHIEIVTNKGII-----GDQV 186
TP + + AK I QS D I+ + N G++ D +
Sbjct 123 ---------------TPAEISKQAKASAAKLIVTQSRYVDKIKNLQNDGVLIVTTDSDAI 167
Query 187 ---CTSFQSLLDTPVTDVQEHYETLDEHQIAKFLFTSGSTKLPKAVPTTH--LMLCINQQ 241
C F L + V E + + F+SG+T LPK V TH L+ + QQ
Sbjct 168 PENCLRFSELTQSEEPRVDSIPEKISPEDVVALPFSSGTTGLPKGVMLTHKGLVTSVAQQ 227
Query 242 MLLQTFPEFEDTPPVLLDWLSWHHTFGGSHNVGIALYNGGTIYIDDGKPVPGKFDET--I 299
+ + + + V+L L H + + + +L G TI I +P KF+ T +
Sbjct 228 VDGENPNLYFNRDDVILCVLPMFHIYALNSIMLCSLRVGATILI-----MP-KFEITLLL 281
Query 300 RNLKEISPTVYLNVPKGWEELTDALEKDAELRDRFFANVKILFFAGAALSEAGWNRLD-K 358
++ TV + VP + A+ K E ++V+++ A L + + + K
Sbjct 282 EQIQRCKVTVAMVVP----PIVLAIAKSPETEKYDLSSVRMVKSGAAPLGKELEDAISAK 337
Query 359 IAQAHCGEKIRIMSGLGMTETAPSCAFTTGPR-----VMAGFIGYPAPGCEIKLV--PYG 411
A G+ G GMTE P A + G V +G G E+K++ G
Sbjct 338 FPNAKLGQ------GYGMTEAGPVLAMSLGFAKEPFPVKSGACGTVVRNAEMKILDPDTG 391
Query 412 DKL------EFCVRGKHVMKGYWRLKADQQSTIFDEDGFFHTGDAVRLVDPND 458
D L E C+RG +MKGY STI D+DG+ HTGD V +D +D
Sbjct 392 DSLPRNKPGEICIRGNQIMKGYLNDPLATASTI-DKDGWLHTGD-VGFIDDDD 442
Score = 21.6 bits (44), Expect = 1.2
Identities = 9/41 (22%), Positives = 21/41 (51%), Gaps = 0/41 (0%)
Query 576 PQLDAGEVTDKGNLNQSNILKRRAALVEELYNKQTDNPLIV 616
P E++ + + + ++ ++ V+++ N Q D LIV
Sbjct 120 PFFTPAEISKQAKASAAKLIVTQSRYVDKIKNLQNDGVLIV 160
>A1L1K7 Long-chain-fatty-acid--CoA ligase ACSBG2 [Rattus norvegicus]
Length=667
Score = 74.3 bits (181), Expect = 5e-17
Identities = 135/627 (22%), Positives = 244/627 (39%), Gaps = 99/627 (16%)
Query 57 YHTFAAKRNTQDEWIRLSYAETLQRAWHIAQALHQRNLSQERPLVILSGNDLEHLTLSMG 116
Y A+K + W L++++ A++L + L + + IL N +E + ++G
Sbjct 63 YPALASKNGKK--WDTLTFSQYYDVCRKAARSLIKLGLQRFHGVGILGFNSVEWVVAALG 120
Query 117 AMLAGVPFSAISPAYSLVSQDFGKLKHVFDVLTPGMVYANDGQAFAKAIQSCTQDHIEIV 176
A+LAG I S + + + +VL + ND Q + I S D +E V
Sbjct 121 AILAGGLCVGIYATNSAEACQYVIKQANVNVL----IVENDQQL--QKILSIPPDKMETV 174
Query 177 TNKGIIGDQV--------CTSFQSLLD--TPVTDVQEHYETLDE--HQIAKFLFTSGSTK 224
K I+ ++ S+Q ++ + ++Q L + +Q A ++TSG+T
Sbjct 175 --KAIVQYRLPLMENSTNLYSWQDFMELGNAIPNIQLDRVILSQKANQCAVIIYTSGTTG 232
Query 225 LPKAVPTTHLML-----CINQQMLLQTFPEFEDTPPVLLDWLSWHHTFGGSHNVGIALYN 279
PK V +H + + +++ L +DT ++ +L H ++ I +
Sbjct 233 SPKGVMLSHDNITWTAGAMAREIELIHVSGKQDT---IVSYLPLSHIAAQLMDIWIPIKV 289
Query 280 GGTIYIDDGKPVPGKFDETIRNLKEISPTVYLNVPKGWEELTDALE----KDAELRDRFF 335
G + + G + L+E+ PT +L VP+ WE++ D ++ K + LR + F
Sbjct 290 GVLTFFAQPDALRGTL---VYTLQEVKPTYFLGVPRVWEKMQDTIKENVAKSSNLRKKAF 346
Query 336 ANVKILFFAGAALSEAGWNRLD---KIAQAHCGEKIRIMSGLGMTETAPSCAFTTGPRVM 392
A K+L G + ++A+A K+R GL T S A V
Sbjct 347 AWAKMLGLKVNTKKMLGKRDIPMNYRMAKALVFTKVRTSLGLDNCHTFFSGASPLSQDVS 406
Query 393 AGFIGYPAPGCEI------------------KLVPYGDKL----------------EFCV 418
F+ P EI +++ G L E C+
Sbjct 407 EFFLSLDIPIGEIYGMTECSGPHTVSCKSIYRVLSCGKVLNGCKNMLYKQNKDGVGEVCM 466
Query 419 RGKHVMKGYWRLKADQQSTIFDEDGFFHTGDAVRLVDPNDPAQGLMYDGRIAEDFKLNTG 478
G+HV GY K D + DEDG+ H+GD RL D L GRI E L T
Sbjct 467 WGRHVFMGYLG-KEDATLEVLDEDGWLHSGDIGRL----DSHDFLYITGRIKE--VLITA 519
Query 479 TFVNVGTLRNKALIQGNL-LIQDVCITGSNLNAIGFLVFPKLDA------------CADF 525
N+ + + L++ + +I + G + L+ K +
Sbjct 520 GGENIWPIPIETLVKEKIPIISHAMLVGDKAKFLSMLLTLKCETDQMSGEPLDKLNLEAI 579
Query 526 AGLNMKNHSAIDILQHPKVQQWFRQFLIQY-----NKDATGSSNRVSMLYLMTEAPQLDA 580
+ M A+ + K++ IQY N+ A S+R+ ++ + +
Sbjct 580 SFCQMLGSQAVTVSDILKIRDPVVYTAIQYGIDIVNQQAVSDSHRIRKWIILEKDFSIQG 639
Query 581 GEVTDKGNLNQSNILKRRAALVEELYN 607
GE+ L + I ++ A ++ +Y+
Sbjct 640 GELGPTSKLKRDLITQKYKAQIDNMYS 666
>P08659 Luciferin 4-monooxygenase [Photinus pyralis]
Length=550
Score = 72.4 bits (176), Expect = 2e-16
Identities = 94/402 (23%), Positives = 166/402 (41%), Gaps = 51/402 (13%)
Query 71 IRLSYAETLQRAWHIAQALHQRNLSQERPLVILSGNDLEHLTLSMGAMLAGVPFSAISPA 130
+ ++YAE + + +A+A+ + L+ +V+ S N L+ +GA+ GV A++PA
Sbjct 49 VNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGV---AVAPA 105
Query 131 YSLVSQDFGKLKHVFDVLTPGMVYANDGQAFAKAIQSCTQDHIEI-VTNKGIIGDQVCT- 188
+ ++ +L + ++ P +V+ + K +Q ++ + K II D
Sbjct 106 NDIYNER--ELLNSMNISQPTVVFVSK-----KGLQKILNVQKKLPIIQKIIIMDSKTDY 158
Query 189 -SFQSLLDTPVTDVQEHYETLD--------EHQIAKFLFTSGSTKLPKAVPTTHLMLCIN 239
FQS+ + + + D + IA + +SGST LPK V H C+
Sbjct 159 QGFQSMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVR 218
Query 240 QQMLLQTFPEFEDTP-PVLLDWLSWHHTFGGSHNVGIALYNGGTIYIDDGKPVPGKFDET 298
+ P +L + +HH FG +G + + + +F+E
Sbjct 219 FSHARDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLM-------YRFEEE 271
Query 299 --IRNLKEISPTVYLNVPKGWEELTDALEKDAELRDRFFANVKILFFAGAALS-EAGWNR 355
+R+L++ L VP L K + +N+ + GA LS E G
Sbjct 272 LFLRSLQDYKIQSALLVP----TLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVG--- 324
Query 356 LDKIAQAHCGEKIRIMSGLGMTETAPSCAFTTGPRVMAGFIGYPAPGCEIKLVP------ 409
+ +A+ IR G G+TET + T G +G P E K+V
Sbjct 325 -EAVAKRFHLPGIR--QGYGLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKT 381
Query 410 --YGDKLEFCVRGKHVMKGYWRLKADQQSTIFDEDGFFHTGD 449
+ E CVRG +M GY + + + D+DG+ H+GD
Sbjct 382 LGVNQRGELCVRGPMIMSGYVN-NPEATNALIDKDGWLHSGD 422
Score = 19.6 bits (39), Expect = 4.8
Identities = 10/27 (37%), Positives = 17/27 (63%), Gaps = 3/27 (11%)
Query 280 GGTIYIDD-GKPVPGKFDETIRNLKEI 305
GG +++D+ K + GK D R ++EI
Sbjct 514 GGVVFVDEVPKGLTGKLD--ARKIREI 538
>P14913 4-coumarate--CoA ligase 2 [Petroselinum crispum]
Length=544
Score = 71.2 bits (173), Expect = 4e-16
Identities = 112/466 (24%), Positives = 186/466 (40%), Gaps = 79/466 (17%)
Query 31 IQPKEQLKAYPQKLTDRLIHFAKVKPYHTFAAKRNTQ------------DEWIRLSYAET 78
+ PKE L + KL D I+ K P HT+ + ++ E S E
Sbjct 5 VAPKEDL-IFRSKLPD--IYIPKHLPLHTYCFENISKVGDKSCLINGATGETFTYSQVEL 61
Query 79 LQRAWHIAQALHQRNLSQERPLVILSGNDLEHLTLSMGAMLAGVPFSAISPAYSLVSQDF 138
L R +A L++ + Q +++L N E+ +GA G + +P +
Sbjct 62 LSRK--VASGLNKLGIQQGDTIMLLLPNSPEYFFAFLGASYRGAISTMANPFF------- 112
Query 139 GKLKHVFDVLTPGMVYANDGQAFAKAI--QSCTQDHI-EIVTNKGI----IGD--QVCTS 189
T V + AK I Q+C D + + K I I D Q C
Sbjct 113 ----------TSAEVIKQLKASLAKLIITQACYVDKVKDYAAEKNIQIICIDDAPQDCLH 162
Query 190 FQSLLDTPVTDVQEHYETLDEHQIAKFLFTSGSTKLPKAVPTTH--LMLCINQQMLLQTF 247
F L++ +++ E +D + ++SG+T LPK V TH L+ + QQ+
Sbjct 163 FSKLMEADESEMPE--VVIDSDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGDNP 220
Query 248 PEFEDTPPVLLDWLSWHHTFGGSHNVGIALYNGGTIYIDDGKPVPGKFDET--IRNLKEI 305
+ + V++ L H + + + L G TI I KFD + +++
Sbjct 221 NLYMHSEDVMICILPLFHIYSLNAVLCCGLRAGVTILIMQ------KFDIVPFLELIQKY 274
Query 306 SPTVYLNVPKGWEELTDALEKDAELRDRFFANVKILFFAGAALSEAGWNRLDKIAQAHCG 365
T+ VP + A+ K + ++V+ + A L + L+ +A
Sbjct 275 KVTIGPFVPP----IVLAIAKSPVVDKYDLSSVRTVMSGAAPLGK----ELEDAVRAKF- 325
Query 366 EKIRIMSGLGMTETAP----SCAFTTGP-RVMAGFIGYPAPGCEIKLV--------PYGD 412
++ G GMTE P AF P + +G G E+K+V P
Sbjct 326 PNAKLGQGYGMTEAGPVLAMCLAFAKEPYEIKSGACGTVVRNAEMKIVDPETNASLPRNQ 385
Query 413 KLEFCVRGKHVMKGYWRLKADQQSTIFDEDGFFHTGDAVRLVDPND 458
+ E C+RG +MKGY ++TI DE+G+ HTGD + +D +D
Sbjct 386 RGEICIRGDQIMKGYLNDPESTRTTI-DEEGWLHTGD-IGFIDDDD 429
>Q96GR2 Long-chain-fatty-acid--CoA ligase ACSBG1 [Homo sapiens]
Length=724
Score = 70.9 bits (172), Expect = 7e-16
Identities = 102/466 (22%), Positives = 182/466 (39%), Gaps = 85/466 (18%)
Query 67 QDEWIRLSYAETLQRAWHIAQALHQRNLSQERPLVILSGNDLEHLTLSMGAMLAGVPFSA 126
QD+W +SY++ A A+ + L Q + IL N E ++G + AG
Sbjct 127 QDKWEHISYSQYYLLARRAAKGFLKLGLKQAHSVAILGFNSPEWFFSAVGTVFAG---GI 183
Query 127 ISPAYSLVSQDFGKLKHVFDVLTPGMVYANDGQAFAKAIQSCTQ-DHIEIVT-------N 178
++ Y+ S + +++ ++ + + K ++ Q H++ V N
Sbjct 184 VTGIYTTSSPE--ACQYIAYDCCANVIMVDTQKQLEKILKIWKQLPHLKAVVIYKEPPPN 241
Query 179 KGIIGDQVCTSFQSL-LDTPVTDVQEHYETLDEHQIAKFLFTSGSTKLPKAVPTTHLMLC 237
K + F L + P + +T +Q ++TSG+T PK V + +
Sbjct 242 K-MANVYTMEEFMELGNEVPEEALDAIIDTQQPNQCCVLVYTSGTTGNPKGVMLSQDNIT 300
Query 238 INQQMLLQT--FPEFEDTPPVLLDWLSWHHTFGGSHNVGIALYNGGTIYIDDGKPVPGKF 295
+ Q E V++ +L H +++ + G + + + G
Sbjct 301 WTARYGSQAGDIRPAEVQQEVVVSYLPLSHIAAQIYDLWTGIQWGAQVCFAEPDALKGSL 360
Query 296 DETIRNLKEISPTVYLNVPKGWEELTDALEKDAELRDRFFANVKILFFAGAALSE----- 350
T+R E+ PT ++ VP+ WE++ + +++ A F K+L +A + E
Sbjct 361 VNTLR---EVEPTSHMGVPRVWEKIMERIQEVAAQSG--FIRRKMLLWAMSVTLEQNLTC 415
Query 351 ------------AGWNRLDKIAQA-----------------------HCGEKIRIMSGLG 375
A + L K+ QA G IR+ +G G
Sbjct 416 PGSDLKPFTTRLADYLVLAKVRQALGFAKCQKNFYGAAPMMAETQHFFLGLNIRLYAGYG 475
Query 376 MTETAPSCAFTTGPRVMAGFIGYP-------APGCEIKLVPYGDKL--EFCVRGKHVMKG 426
++ET+ GP M+ Y PGC +KLV + E C+ G+ + G
Sbjct 476 LSETS-------GPHFMSSPYNYRLYSSGKLVPGCRVKLVNQDAEGIGEICLWGRTIFMG 528
Query 427 YWRLKADQQSTIFDEDGFFHTGDAVRLVDPNDPAQGLMY-DGRIAE 471
Y ++ D+ DE+G+ HTGDA RL A G +Y GR+ E
Sbjct 529 YLNME-DKTCEAIDEEGWLHTGDAGRL-----DADGFLYITGRLKE 568
>Q5FVE4 Long-chain-fatty-acid--CoA ligase ACSBG2 [Homo sapiens]
Length=666
Score = 70.1 bits (170), Expect = 1e-15
Identities = 128/627 (20%), Positives = 251/627 (40%), Gaps = 101/627 (16%)
Query 57 YHTFAAKRNTQDEWIRLSYAETLQRAWHIAQALHQRNLSQERPLVILSGNDLEHLTLSMG 116
Y A+K + W L++ + + A++L + L + + IL N E ++G
Sbjct 67 YPALASKNGKK--WEILNFNQYYEACRKAAKSLIKLGLERFHGVGILGFNSAEWFITAVG 124
Query 117 AMLAGVPFSAISPAYSLVSQDFGKLKHVFDVLTPGMVYANDGQAFAKAIQSCTQDHIEIV 176
A+LAG I Y+ S + ++V ++ + Q K I S Q +E +
Sbjct 125 AILAGGLCVGI---YATNSAEV--CQYVITHAKVNILLVENDQQLQK-ILSIPQSSLEPL 178
Query 177 TNKGIIGDQVCTSFQSLL-----------DTPVTDVQEHYETLDEHQIAKFLFTSGSTKL 225
K II ++ + L P T +++ E+ +Q A ++TSG+T +
Sbjct 179 --KAIIQYRLPMKKNNNLYSWDDFMELGRSIPDTQLEQVIESQKANQCAVLIYTSGTTGI 236
Query 226 PKAVPTTHLMLCINQQMLLQTFPEFEDTPPVLLDWLSWHHTFGGSHNVGIALYNGGTIYI 285
PK V +H + + + F + D ++ +L H ++ + + G Y
Sbjct 237 PKGVMLSHDNITWIAGAVTKDF-KLTDKHETVVSYLPLSHIAAQMMDIWVPIKIGALTYF 295
Query 286 DDGKPVPGKFDETIRNLKEISPTVYLNVPKGWEELTDALEKDAE----LRDRFFA---NV 338
+ G T LKE+ PTV++ VP+ WE++ + ++K++ L+ + F N+
Sbjct 296 AQADALKGTLVST---LKEVKPTVFIGVPQIWEKIHEMVKKNSAKSMGLKKKAFVWARNI 352
Query 339 KILFFAGAALSEAGWNRLDKIAQAHCGEKIRIMSGLG-----MTETAP------------ 381
+ L + ++A+ K++ GL ++ TAP
Sbjct 353 GFKVNSKKMLGKYNTPVSYRMAKTLVFSKVKTSLGLDHCHSFISGTAPLNQETAEFFLSL 412
Query 382 --------SCAFTTGPRVMAGFIGYPAPGCEIKLVPYGDKL---------EFCVRGKHVM 424
+ ++GP ++ Y C L + L E C+ G+H+
Sbjct 413 DIPIGELYGLSESSGPHTISNQNNYRLLSCGKILTGCKNMLFQQNKDGIGEICLWGRHIF 472
Query 425 KGYWRLKADQQST-IFDEDGFFHTGDAVRLVDPNDPAQGLMYDGRIAEDFKLNTGTFVNV 483
GY L+++ ++T D++G+ H+GD +L D L G I E L T NV
Sbjct 473 MGY--LESETETTEAIDDEGWLHSGDLGQL----DGLGFLYVTGHIKE--ILITAGGENV 524
Query 484 GTLRNKALIQGNL-LIQDVCITGSNLNAIGFLVFPK---------------LDACADFAG 527
+ + L++ + +I + + G L + L+ K +A G
Sbjct 525 PPIPVETLVKKKIPIISNAMLVGDKLKFLSMLLTLKCEMNQMSGEPLDKLNFEAINFCRG 584
Query 528 LNMKNHSAIDIL--QHPKVQQWFRQFLIQYNKDATGSSNRVSMLYLMTEAPQLDAGEVTD 585
L + + +I+ Q P V + +Q + N++A ++ R+ ++ + + GE+
Sbjct 585 LGSQASTVTEIVKQQDPLVYKAIQQGINAVNQEAMNNAQRIEKWVILEKDFSIYGGEL-- 642
Query 586 KGNLNQSNILKRRAALVEELYNKQTDN 612
++K + V + Y KQ D+
Sbjct 643 ------GPMMKLKRHFVAQKYKKQIDH 663
>Q9S777 4-coumarate--CoA ligase 3 [Arabidopsis thaliana]
Length=561
Score = 69.7 bits (169), Expect = 1e-15
Identities = 94/401 (23%), Positives = 164/401 (41%), Gaps = 41/401 (10%)
Query 74 SYAETLQRAWHIAQALHQRNLSQERPLVILSGNDLEHLTLSMGAMLAGVPFSAISPAYSL 133
+Y ET +A L++ + + ++IL N E + MGA + G + +P Y+
Sbjct 77 TYGETHLICRRVASGLYKLGIRKGDVIMILLQNSAEFVFSFMGASMIGAVSTTANPFYT- 135
Query 134 VSQDFGKLKHVFDVLTPGMVYANDGQAFAKAIQSCTQDHIEIVTNKGIIGDQVCTSFQSL 193
SQ+ K + + G + +++ ++ I T++ + C F +L
Sbjct 136 -SQELYK-----QLKSSGAKLIITHSQYVDKLKNLGENLTLITTDEPT--PENCLPFSTL 187
Query 194 LDTPVTDVQEHYETLDEHQIAKFLFTSGSTKLPKAVPTTH--LMLCINQQMLLQTFPEFE 251
+ T+ + + A F+SG+T LPK V TH L+ + QQ+ +
Sbjct 188 ITDDETNPFQETVDIGGDDAAALPFSSGTTGLPKGVVLTHKSLITSVAQQVDGDNPNLYL 247
Query 252 DTPPVLLDWLSWHHTFGGSHNVGIALYNGGTIYIDDGKPVPGKFDETIRNLKEISPTVYL 311
+ V+L L H + + + +L +G T+ + + D R+ I+ V
Sbjct 248 KSNDVILCVLPLFHIYSLNSVLLNSLRSGATVLLMHKFEIGALLDLIQRHRVTIAALV-- 305
Query 312 NVPKGWEELTDALEKDAELRDRFFANVKILFFAGAALSEAGWNRL-DKIAQAHCGEKIRI 370
L AL K+ + ++V+ + A L + + L ++ QA G+
Sbjct 306 ------PPLVIALAKNPTVNSYDLSSVRFVLSGAAPLGKELQDSLRRRLPQAILGQ---- 355
Query 371 MSGLGMTETAP----SCAFTTGP-RVMAGFIGYPAPGCEIKLV--------PYGDKLEFC 417
G GMTE P S F P +G G E+K+V Y E C
Sbjct 356 --GYGMTEAGPVLSMSLGFAKEPIPTKSGSCGTVVRNAELKVVHLETRLSLGYNQPGEIC 413
Query 418 VRGKHVMKGYWRLKADQQSTIFDEDGFFHTGDAVRLVDPND 458
+RG+ +MK Y + S DE+G+ HTGD + VD +D
Sbjct 414 IRGQQIMKEYLN-DPEATSATIDEEGWLHTGD-IGYVDEDD 452
Score = 19.6 bits (39), Expect = 4.8
Identities = 17/77 (22%), Positives = 33/77 (43%), Gaps = 2/77 (3%)
Query 85 IAQALHQRNLSQERPLVILSGNDLEHLTLSMGAMLAGVPFSAISPAYSLVSQDFGKLKHV 144
I ALH+ + + ++S + L H + + +P I L + F KL V
Sbjct 2 ITAALHEPQIHKPTDTSVVSDDVLPHSPPTPRIFRSKLPDIDIPNHLPLHTYCFEKLSSV 61
Query 145 FDVLTPGMVYANDGQAF 161
D P ++ + G+++
Sbjct 62 SD--KPCLIVGSTGKSY 76
>M4IS90 Probable CoA ligase CCL8 [Humulus lupulus]
Length=607
Score = 69.7 bits (169), Expect = 1e-15
Identities = 81/329 (25%), Positives = 137/329 (42%), Gaps = 42/329 (13%)
Query 199 TDVQEHYETLDEHQIAKFLFTSGSTKLPKAVPTTHLMLCINQQMLLQTFPEFEDTPP-VL 257
TD H + A ++TSG+T PK V TH + Q L + + E TP
Sbjct 216 TDSILHNTEISNENPALIVYTSGTTGKPKGVVHTHKSINAQVQTLAKAW---EYTPADQF 272
Query 258 LDWLSWHHTFGGSHNVGIALYNGGTIYIDDGKPVPG--------------KFDETIRNLK 303
L L HH G + + LY T+ V G K D+ I
Sbjct 273 LHCLPLHHVHGLFNALFAPLYARSTVEFMPKFSVRGIWQRWRESYPTCETKVDDCITVFT 332
Query 304 EISPTVYLNVPKGWEELTDALEKDAELRDRFFANVKILFFAGAALSEAGWNRLDKIAQAH 363
+ PT+Y + +G+E + L++ + + ++++ +AL + I H
Sbjct 333 GV-PTMYTRLIQGYEAMDPELKEASASAAK---QLRLMMCGSSALPIPVMQQWQTIT-GH 387
Query 364 CGEKIRIMSGLGMTETAPSCAFTTGPRVMAGFIGYPAPGCEIKLVPYGDKL-------EF 416
R++ GMTE + + AG +G P PG E+++V + E
Sbjct 388 -----RLLERYGMTEFVMAISNPLKGERKAGTVGKPFPGVEVRIVAEDENGSDTTGVGEL 442
Query 417 CVRGKHVMKGYWRLKADQQSTIFDEDGFFHTGDAVRLVDPNDPAQGLMYDGRIAEDFKLN 476
CVR + K YWRL +S+ F +DGFF TGDA ++ D + GR + D +
Sbjct 443 CVRSPSLFKEYWRLPEVTKSS-FTDDGFFKTGDAGKV----DEDGYYVILGRTSADI-MK 496
Query 477 TGTFVNVGTLRNKALIQGNLLIQDVCITG 505
G + + L ++++ + +++ C+ G
Sbjct 497 VGGY-KLSALEIESVLLEHPTVEECCVLG 524
Score = 18.9 bits (37), Expect = 8.4
Identities = 10/21 (48%), Positives = 13/21 (62%), Gaps = 1/21 (5%)
Query 255 PVLLDWLSWHHTFGGSHNVGI 275
P L++ LS H GG+ VGI
Sbjct 104 PALINGLSGHGHLGGA-RVGI 123
>Q4R4P9 Long-chain-fatty-acid--CoA ligase ACSBG1 [Macaca fascicularis]
Length=724
Score = 69.7 bits (169), Expect = 2e-15
Identities = 101/466 (22%), Positives = 182/466 (39%), Gaps = 85/466 (18%)
Query 67 QDEWIRLSYAETLQRAWHIAQALHQRNLSQERPLVILSGNDLEHLTLSMGAMLAGVPFSA 126
QD+W +SY++ A A+ + L + + IL N E ++G + AG
Sbjct 127 QDKWEHISYSQYYLLARRAAKGFLKLGLERAHSVAILGFNSPEWFFSAVGTVFAG---GI 183
Query 127 ISPAYSLVSQDFGKLKHVFDVLTPGMVYANDGQAFAKAIQSCTQ-DHIEIVT-------N 178
++ Y+ S + +++ ++ + + K ++ Q H++ V N
Sbjct 184 VTGIYTTSSPE--ACQYIAYDCCANVIMVDTQKQLEKILKVWKQLPHLKAVVIYKEPPPN 241
Query 179 KGIIGDQVCTSFQSL-LDTPVTDVQEHYETLDEHQIAKFLFTSGSTKLPKAVPTTHLMLC 237
K + F L + P + +T +Q ++TSG+T PK V + +
Sbjct 242 K-MANVYTMEEFMELGNEVPEEALDAIIDTQQPNQCCVLVYTSGTTGNPKGVMLSQDNIT 300
Query 238 INQQMLLQT--FPEFEDTPPVLLDWLSWHHTFGGSHNVGIALYNGGTIYIDDGKPVPGKF 295
+ Q E V++ +L H +++ + G + + + G
Sbjct 301 WTARYGSQAGDIRPAEVQQEVVVSYLPLSHIAAQIYDLWTGIQWGAQVCFAEPDALKGSL 360
Query 296 DETIRNLKEISPTVYLNVPKGWEELTDALEKDAELRDRFFANVKILFFAGAALSE----- 350
T+R E+ PT ++ VP+ WE++ + +++ A F K+L +A + E
Sbjct 361 VNTLR---EVEPTSHMGVPRVWEKIMERIQEVAAQSG--FIRRKMLLWAMSVTLEQNLTC 415
Query 351 ------------AGWNRLDKIAQA-----------------------HCGEKIRIMSGLG 375
A + L K+ QA G IR+ +G G
Sbjct 416 PGSDLKPFTTRLADYLVLAKVRQALGFAKCQKNFYGAAPMTAETQHFFLGLNIRLYAGYG 475
Query 376 MTETAPSCAFTTGPRVMAGFIGYP-------APGCEIKLVPYGDKL--EFCVRGKHVMKG 426
++ET+ GP M+ Y PGC +KLV + E C+ G+ + G
Sbjct 476 LSETS-------GPHFMSSPCNYRLYSSGKLVPGCRVKLVNQDTEGIGEICLWGRTIFMG 528
Query 427 YWRLKADQQSTIFDEDGFFHTGDAVRLVDPNDPAQGLMY-DGRIAE 471
Y ++ D+ DE+G+ HTGDA RL A G +Y GR+ E
Sbjct 529 YLNME-DKTCEAIDEEGWLHTGDAGRL-----DADGFLYITGRLKE 568
>P14912 4-coumarate--CoA ligase 1 [Petroselinum crispum]
Length=544
Score = 69.3 bits (168), Expect = 2e-15
Identities = 112/464 (24%), Positives = 185/464 (40%), Gaps = 75/464 (16%)
Query 31 IQPKEQLKAYPQKLTDRLIHFAKVKPYHTFAAKRNTQ------------DEWIRLSYAET 78
+ PKE L + KL D I+ K P HT+ + ++ E S E
Sbjct 5 VAPKEDL-IFRSKLPD--IYIPKHLPLHTYCFENISKVGDKSCLINGATGETFTYSQVEL 61
Query 79 LQRAWHIAQALHQRNLSQERPLVILSGNDLEHLTLSMGAMLAGVPFSAISPAYSLVSQDF 138
L R +A L++ + Q +++L N E+ +GA G A S ++ F
Sbjct 62 LSRK--VASGLNKLGIQQGDTIMLLLPNSPEYFFAFLGASYRG--------AISTMANPF 111
Query 139 GKLKHVFDVLTPGMVYANDGQAFAKAIQSCTQDHI-EIVTNKGI----IGD--QVCTSFQ 191
V L QA Q+C D + + K I I D Q C F
Sbjct 112 FTSAEVIKQLKAS-------QAKLIITQACYVDKVKDYAAEKNIQIICIDDAPQDCLHFS 164
Query 192 SLLDTPVTDVQEHYETLDEHQIAKFLFTSGSTKLPKAVPTTH--LMLCINQQMLLQTFPE 249
L++ +++ E ++ + ++SG+T LPK V TH L+ + QQ+
Sbjct 165 KLMEADESEMPE--VVINSDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGDNPNL 222
Query 250 FEDTPPVLLDWLSWHHTFGGSHNVGIALYNGGTIYIDDGKPVPGKFDET--IRNLKEISP 307
+ + V++ L H + + + L G TI I KFD + +++
Sbjct 223 YMHSEDVMICILPLFHIYSLNAVLCCGLRAGVTILIMQ------KFDIVPFLELIQKYKV 276
Query 308 TVYLNVPKGWEELTDALEKDAELRDRFFANVKILFFAGAALSEAGWNRLDKIAQAHCGEK 367
T+ VP + A+ K + ++V+ + A L + L+ +A
Sbjct 277 TIGPFVPP----IVLAIAKSPVVDKYDLSSVRTVMSGAAPLGK----ELEDAVRAKF-PN 327
Query 368 IRIMSGLGMTETAP----SCAFTTGP-RVMAGFIGYPAPGCEIKLV--------PYGDKL 414
++ G GMTE P AF P + +G G E+K+V P +
Sbjct 328 AKLGQGYGMTEAGPVLAMCLAFAKEPYEIKSGACGTVVRNAEMKIVDPETNASLPRNQRG 387
Query 415 EFCVRGKHVMKGYWRLKADQQSTIFDEDGFFHTGDAVRLVDPND 458
E C+RG +MKGY ++TI DE+G+ HTGD + +D +D
Sbjct 388 EICIRGDQIMKGYLNDPESTRTTI-DEEGWLHTGD-IGFIDDDD 429
Score = 21.2 bits (43), Expect = 1.6
Identities = 15/60 (25%), Positives = 23/60 (38%), Gaps = 8/60 (13%)
Query 448 GDAVRLVDPNDPAQ-----GLMYDGRIAEDFKLNTGTFVNVGTLRNKALIQGNLLIQDVC 502
GD + L+ PN P G Y G I+ + F + ++ Q L+I C
Sbjct 78 GDTIMLLLPNSPEYFFAFLGASYRGAIS---TMANPFFTSAEVIKQLKASQAKLIITQAC 134
>A0A2H5AIX5 4-coumarate-CoA ligase 1 [Narcissus pseudonarcissus]
Length=547
Score = 68.9 bits (167), Expect = 2e-15
Identities = 104/412 (25%), Positives = 170/412 (41%), Gaps = 61/412 (15%)
Query 73 LSYAETLQRAWHIAQALHQRNLSQERPLVILSGNDLEHLTLSMGAMLAGVPFSAISPAYS 132
L+YAE + + LH+ L Q +++L N +E + +GA L G + +P Y
Sbjct 53 LTYAEVELASKRVGAGLHRLGLRQGHVVMLLLPNSIEFVLSFIGASLLGAMSTTANPFY- 111
Query 133 LVSQDFGKLKHVFDVLTPGMVYANDGQAFAKAI--QSC---TQDHIEIVTNKGIIGD--Q 185
T ++ A AK I +SC +E ++ +I D +
Sbjct 112 ----------------TSAEIHKQAAAAGAKIIVTESCHVSKLQGLEGISRIVVIDDAVR 155
Query 186 VCTSFQSLLDTPVTDVQEHYETLDEH--QIAKFLFTSGSTKLPKAVPTTH--LMLCINQQ 241
V + + TD E +D H + ++SG+T LPK V TH L+ + QQ
Sbjct 156 VPENVMHFSELESTDEAE-LPRIDVHPDDVVALPYSSGTTGLPKGVMLTHNGLVTSVAQQ 214
Query 242 MLLQTFPEFEDTPPVLLDWLSWHHTFGGSHNVGIALYNGGTIYIDDGKPVPGKFDETIRN 301
+ + VLL L H + + + L G I + KF+ I
Sbjct 215 VDGENPNLHFSEDDVLLCVLPLFHIYSLNSVLLCGLRAGAAIVL------MRKFE--IVR 266
Query 302 LKEISPTVYLNVPKGWEELTDALEKDAELRDRF-FANVKILFFAGAALSEAGWNRL-DKI 359
L E+ + + + + K+ E DR+ ++++++ A + + N+L +K+
Sbjct 267 LMELVEKYRVTIAPFVPPIVVEMVKN-EAVDRYDLSSIRVVMSGAAPMGKELENKLREKL 325
Query 360 AQAHCGEKIRIMSGLGMTETAP----SCAFTTGP-RVMAGFIGYPAPGCEIKLV--PYGD 412
A G+ G GMTE P AF P V +G G E+K++ G
Sbjct 326 PNAKLGQ------GYGMTEAGPVLSMCLAFAKEPFEVKSGSCGTVVRNAELKIIDPETGF 379
Query 413 KL------EFCVRGKHVMKGYWRLKADQQSTIFDEDGFFHTGDAVRLVDPND 458
L E C+RG +MKGY + TI DE+G+ HTGD + VD +D
Sbjct 380 SLSRNQPGEICIRGNQIMKGYLNNPEATKQTI-DEEGWLHTGD-IGFVDDDD 429
>Q9LK39 Probable acyl-activating enzyme 16, chloroplastic [Arabidopsis
thaliana]
Length=722
Score = 67.8 bits (164), Expect = 6e-15
Identities = 61/262 (23%), Positives = 113/262 (43%), Gaps = 37/262 (14%)
Query 368 IRIMSGLGMTETAPSCAFTTGPRVMAGFIGYPAPGCEIKLV--------PYGDKLEFCVR 419
+ + +G G+TET+P + + G +G+P E K+V P G K VR
Sbjct 474 VNVQNGYGLTETSPVVSARRLRCNVLGSVGHPIKDTEFKIVDHETGTVLPPGSKGIVKVR 533
Query 420 GKHVMKGYWRLKADQQSTIFDEDGFFHTGDAVRLVDPNDPAQG------LMYDGRIAEDF 473
G VMKGY++ + I D+DG+F+TGD + + + ++ +GR +
Sbjct 534 GPPVMKGYYKNPLATKQVI-DDDGWFNTGDMGWITPQHSTGRSRSCGGVIVLEGRAKDTI 592
Query 474 KLNTGTFVNVGTLRNKALIQGNLLIQDVCITGSNLNAIGFLVFPKLDACADFA------- 526
L+TG NV L + + LIQ + + G + +G +V P +A A
Sbjct 593 VLSTGE--NVEPLEIEEAAMRSNLIQQIVVIGQDQRRLGAIVIPNKEAAEGAAKQKISPV 650
Query 527 --GLNMKNHSAIDILQHPKVQQWFRQFLIQYNKDATGSSNRVSMLYLMTEAPQLDAGEVT 584
+N + I + + ++++W Q S +V + ++ E +D G +T
Sbjct 651 DSEVNELSKETITSMVYEELRKWTSQ-----------CSFQVGPVLIVDEPFTIDNGLMT 699
Query 585 DKGNLNQSNILKRRAALVEELY 606
+ + ++ + +E LY
Sbjct 700 PTMKIRRDKVVDQYKNEIERLY 721
Score = 34.3 bits (77), Expect = 2e-04
Identities = 23/60 (38%), Positives = 29/60 (48%), Gaps = 1/60 (2%)
Query 205 YETLDEHQIAKFLFTSGSTKLPKAVPTTHLMLCINQQMLLQTFPEFEDTPPVLLDWLSWH 264
YE +D IA ++TSG+T PK V TH L ++Q L F E L SWH
Sbjct 257 YEYIDPDDIATIMYTSGTTGNPKGVMLTHQNL-LHQIRNLSDFVPAEAGERFLSMLPSWH 315
Score = 18.9 bits (37), Expect = 8.5
Identities = 9/19 (47%), Positives = 11/19 (58%), Gaps = 1/19 (5%)
Query 448 GDAVRLVDP-NDPAQGLMY 465
GD V +VDP +DP Y
Sbjct 95 GDRVAVVDPYHDPPSTFTY 113
Lambda K H a alpha
0.322 0.137 0.418 0.792 4.96
Gapped
Lambda K H a alpha sigma
0.267 0.0410 0.140 1.90 42.6 43.6
Effective search space used: 3848640
Database: d95dce22a6b07c9e4317008fa7aa2158.SwissProt.fasta
Posted date: Jun 2, 2024 10:33 AM
Number of letters in database: 7,412
Number of sequences in database: 12
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 11
Window for multiple hits: 40
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Data ...
