BLASTP 2.12.0+


Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.


Reference for composition-based statistics: Alejandro A. Schaffer,
L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri
I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids
Res. 29:2994-3005.



Database: 88dcb832f87366e7c67d3ac78f7c0a8a.SwissProt.fasta
           1 sequences; 2,875 total letters



Query= ACIAD2031

Length=282
                                                                      Score     E
Sequences producing significant alignments:                          (Bits)  Value

P28976 cap-snatching endonuclease [Tomato spotted wilt virus (str...  28.9    0.001


>P28976 cap-snatching endonuclease [Tomato spotted wilt virus 
(strain Brazilian Br-01)]
Length=2875

 Score = 28.9 bits (63),  Expect = 0.001
 Identities = 30/104 (29%), Positives = 46/104 (44%), Gaps = 22/104 (21%)

Query  75    ILANLNQPVIYST----------PKSKPIINILGRYYDVNQQVNIKIQSILESLKLYLDL  124
             +L N  +P I+ST          P  K    +   Y +VN  +N     I +SL+ YL L
Sbjct  2705  LLRNSFRPDIFSTDRLGRLSSSVPALKVYATVYMEYKNVNCPLN----EIADSLEGYLKL  2760

Query  125   TESNDDIDFLFKALIQALCQTDLDTLFLITDIRFDEKKLQLLQD  168
             T+S     FL   + +AL Q        + D +   KKL++ +D
Sbjct  2761  TKSRSKEHFLSGRVKKALIQ--------LRDEQSRTKKLEVYKD  2796


 Score = 22.3 bits (46),  Expect = 0.12
 Identities = 13/50 (26%), Positives = 24/50 (48%), Gaps = 1/50 (2%)

Query  210   KSFSEMNANLVVSTDLFTHQQATHLVEDMFYSE-HIFEKLSIYAEYIQTH  258
             K FS++  NL  + DL+   +   + ED    E  +++K++   E    H
Sbjct  1771  KDFSKLYPNLKKNEDLYKSTKNLKIDEDAVLEEDELYKKIASSLEMESVH  1820


 Score = 18.5 bits (36),  Expect = 1.9
 Identities = 11/40 (28%), Positives = 20/40 (50%), Gaps = 3/40 (8%)

Query  242  EHIFEKLSIYAEYIQTHIQ---NDQALGLSLIQKSHLRRL  278
            E I E++S   EY  +  Q   ND    + +   +H+++L
Sbjct  616  EEIKERISKGLEYDTSERQADPNDDYSSIDMSSLTHMKKL  655


 Score = 18.5 bits (36),  Expect = 1.9
 Identities = 13/40 (33%), Positives = 19/40 (48%), Gaps = 3/40 (8%)

Query  166  LQDFLDIKIYVISTSKHTALLKDHPL---SLKKLLFKSKD  202
            L D+ +IK Y+   S          L    +KKLLF+ +D
Sbjct  910  LSDYSNIKEYIRDKSDPDITNCGRYLFRNGIKKLLFRMED  949


 Score = 18.1 bits (35),  Expect = 2.5
 Identities = 5/14 (36%), Positives = 10/14 (71%), Gaps = 0/14 (0%)

Query  205  HRNVCKSFSEMNAN  218
            ++N+ KSF ++  N
Sbjct  203  YKNIWKSFKDIKVN  216


 Score = 18.1 bits (35),  Expect = 2.5
 Identities = 8/27 (30%), Positives = 15/27 (56%), Gaps = 0/27 (0%)

Query  103   VNQQVNIKIQSILESLKLYLDLTESND  129
             +N+    K+ S  +  KLY +L ++ D
Sbjct  1759  INENALNKMSSYKDFSKLYPNLKKNED  1785


 Score = 17.3 bits (33),  Expect = 4.3
 Identities = 7/17 (41%), Positives = 9/17 (53%), Gaps = 0/17 (0%)

Query  188   DHPLSLKKLLFKSKDDF  204
             DH L L+  L +   DF
Sbjct  2587  DHDLDLQNNLMEKSYDF  2603


 Score = 16.9 bits (32),  Expect = 5.6
 Identities = 9/26 (35%), Positives = 15/26 (58%), Gaps = 1/26 (4%)

Query  115   LESLKLYLDLTESNDDIDFLFKALIQ  140
             +E    YLD    ND ++ LF+ L++
Sbjct  2446  VEEYDSYLDENGFNDTVE-LFENLLR  2470


 Score = 16.5 bits (31),  Expect = 7.4
 Identities = 12/42 (29%), Positives = 20/42 (48%), Gaps = 15/42 (36%)

Query  12    QIFLDREPSKLMQ--NQALQ-------------TYVNLEATL  38
             Q+F+   PSK  Q  NQ+ +             T+VN+ +T+
Sbjct  1844  QLFMYTSPSKRNQLSNQSTEKLALDRVLRSKARTFVNISSTV  1885


 Score = 16.2 bits (30),  Expect = 9.8
 Identities = 8/26 (31%), Positives = 15/26 (58%), Gaps = 0/26 (0%)

Query  93   IINILGRYYDVNQQVNIKIQSILESL  118
            IIN   + Y+  +++ +KI +  E L
Sbjct  47   IINNNAKNYETMRELIVKITADGEGL  72


 Score = 16.2 bits (30),  Expect = 9.8
 Identities = 7/27 (26%), Positives = 13/27 (48%), Gaps = 0/27 (0%)

Query  101   YDVNQQVNIKIQSILESLKLYLDLTES  127
             Y   + + I   ++LE  +LY  +  S
Sbjct  1787  YKSTKNLKIDEDAVLEEDELYKKIASS  1813


 Score = 16.2 bits (30),  Expect = 9.8
 Identities = 6/19 (32%), Positives = 10/19 (53%), Gaps = 0/19 (0%)

Query  197   LFKSKDDFHRNVCKSFSEM  215
             L ++ D+F       FSE+
Sbjct  2468  LLRTHDNFENEYSPLFSEI  2486



Lambda      K        H        a         alpha
   0.325    0.139    0.383    0.792     4.96 

Gapped
Lambda      K        H        a         alpha    sigma
   0.267   0.0410    0.140     1.90     42.6     43.6 

Effective search space used: 679920


  Database: 88dcb832f87366e7c67d3ac78f7c0a8a.SwissProt.fasta
    Posted date:  Jun 2, 2024  5:30 AM
  Number of letters in database: 2,875
  Number of sequences in database:  1



Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 11
Window for multiple hits: 40