BLASTP 2.12.0+
Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics: Alejandro A. Schaffer,
L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri
I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids
Res. 29:2994-3005.
Database: b9f31ddf269067f4d0daab37281bd5f0.SwissProt.fasta
2 sequences; 806 total letters
Query= ACIAD2137
Length=242
Score E
Sequences producing significant alignments: (Bits) Value
Q5KW02 S-adenosylmethionine synthase [Geobacillus kaustophilus (s... 37.7 3e-07
C5D6E9 S-adenosylmethionine synthase [Geobacillus sp. (strain WCH... 35.4 2e-06
>Q5KW02 S-adenosylmethionine synthase [Geobacillus kaustophilus
(strain HTA426)]
Length=403
Score = 37.7 bits (86), Expect = 3e-07
Identities = 30/98 (31%), Positives = 45/98 (46%), Gaps = 7/98 (7%)
Query 16 EISELVQSRPDKVKQSIERLAEKGVITLPPMVETSFLNSIGRKQNTTVYKFTGEQGKRDS 75
E EL+ + RLAE + P +L G+ Q T Y G+ + D+
Sbjct 143 ETEELMPMPISLAHRLARRLAEVRKTDVLP-----YLRPDGKTQVTIEYDENGKPVRVDT 197
Query 76 IIVVAQLCPEFTARLVDRWQELEAQVAKPVDPMQALSD 113
I+V AQ PE T + R +++ QV KPV P + L +
Sbjct 198 IVVSAQHHPEITQEQIQR--DIKEQVIKPVVPAELLDE 233
Score = 26.9 bits (58), Expect = 8e-04
Identities = 17/69 (25%), Positives = 30/69 (43%), Gaps = 2/69 (3%)
Query 129 VELEHKVEVMQPTIDAFDRIATANGS--LCLTDAAKALQMRPKDFISFLSQKQWIYKRTG 186
V+L + + V +P + D T S + + +RP I L ++ IYK+T
Sbjct 311 VQLAYAIGVARPVSISIDTFGTGKVSEDILIEVVRNNFDLRPAGIIKMLDLRRPIYKQTA 370
Query 187 SAHYVGYQD 195
+ + G D
Sbjct 371 AYGHFGRTD 379
>C5D6E9 S-adenosylmethionine synthase [Geobacillus sp. (strain
WCH70)]
Length=403
Score = 35.4 bits (80), Expect = 2e-06
Identities = 19/63 (30%), Positives = 34/63 (54%), Gaps = 2/63 (3%)
Query 51 FLNSIGRKQNTTVYKFTGEQGKRDSIIVVAQLCPEFTARLVDRWQELEAQVAKPVDPMQA 110
+L G+ Q T Y G+ + D+I+V AQ PE T ++R +++ V KP+ P +
Sbjct 173 YLRPDGKTQVTVEYDENGKPVRIDTIVVSAQHHPEITQEQIER--DIKEHVIKPIVPAEL 230
Query 111 LSD 113
+ +
Sbjct 231 IDE 233
Score = 26.9 bits (58), Expect = 8e-04
Identities = 17/69 (25%), Positives = 30/69 (43%), Gaps = 2/69 (3%)
Query 129 VELEHKVEVMQPTIDAFDRIATANGS--LCLTDAAKALQMRPKDFISFLSQKQWIYKRTG 186
V+L + + V +P + D T S + + +RP I L ++ IYK+T
Sbjct 311 VQLAYAIGVARPVSISIDTFGTGKVSEDILIEVVRNNFDLRPAGIIKMLDLRRPIYKQTA 370
Query 187 SAHYVGYQD 195
+ + G D
Sbjct 371 AYGHFGRTD 379
Lambda K H a alpha
0.319 0.133 0.378 0.792 4.96
Gapped
Lambda K H a alpha sigma
0.267 0.0410 0.140 1.90 42.6 43.6
Effective search space used: 155820
Database: b9f31ddf269067f4d0daab37281bd5f0.SwissProt.fasta
Posted date: Jun 1, 2024 11:16 PM
Number of letters in database: 806
Number of sequences in database: 2
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 11
Window for multiple hits: 40