BLASTP 2.12.0+


Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.


Reference for composition-based statistics: Alejandro A. Schaffer,
L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri
I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids
Res. 29:2994-3005.



Database: 464b597d11387c910ce144a513523f91.SwissProt.fasta
           1 sequences; 886 total letters



Query= ACIAD2183

Length=301
                                                                      Score     E
Sequences producing significant alignments:                          (Bits)  Value

O33600 DNA double-strand break repair Rad50 ATPase [Sulfolobus ac...  40.0    1e-07


>O33600 DNA double-strand break repair Rad50 ATPase [Sulfolobus 
acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 
15157 / NCIMB 11770)]
Length=886

 Score = 40.0 bits (92),  Expect = 1e-07
 Identities = 49/203 (24%), Positives = 92/203 (45%), Gaps = 50/203 (25%)

Query  104  EQYQALKEIFDKLPELQQQFEEVLEQTGRFTFID------------IKMGIKALQNDAFD  151
            EQ Q L E+  KL EL++ + +      ++ F+             I+  I  L+    +
Sbjct  566  EQEQRLNELNSKLSELEKSYNDY---KAKYQFLPADLKSLVSLEERIRRRISELEKLKIE  622

Query  152  YWMNQMIKAKITS-KGLITE-EQFKTEQEKEIDEEFSQGWGKKQYVRDTKTVTLAVIDEY  209
            Y   + +K +IT  KGL  E E+ K E++  ++     G+ +K+Y +  +     +ID+ 
Sbjct  623  Y---ERLKEEITRMKGLKEEYEKLKEEEDALLNRISELGYSEKRYKQLEE-----IIDKL  674

Query  210  DLVAQWHEAEKLKVYFERIHREIRNPAGMPNDLFKKLIKDLKEYEHNLKELKNFCIRGNR  269
              +    EA+K K+                    ++ IK+++E E N++EL+N       
Sbjct  675  SKILSGIEADKGKI----------------KGSLEEKIKNIEEKERNIEELRN-------  711

Query  270  LLTQENLMKLGILFKKPDELRKV  292
             + +E+ + LGI   K  +LR+V
Sbjct  712  KMNEESKLNLGI--SKLQKLREV  732


 Score = 32.7 bits (73),  Expect = 2e-05
 Identities = 39/187 (21%), Positives = 74/187 (40%), Gaps = 25/187 (13%)

Query  82   RNNFGVDFTNKEREFNTQRIHAEQYQALKEIFDKLPE-------LQQQFEEVLEQTGRFT  134
            RN   ++   + +E        ++ Q L+E  +KL           Q+FE + E+     
Sbjct  488  RNKLQLELQKRSKEKGIYEAKLKELQRLEEEKNKLQNEILSLLSYHQEFENIAEK-----  542

Query  135  FIDIKMGIKALQNDAFDYWMNQMIKAKITSKGLITEEQFKTEQEKEIDE-EFSQGWGKKQ  193
                       + +  DY    +  + I  + +  +EQ   E   ++ E E S    K +
Sbjct  543  -----------EKELIDYHEEYLKNSDILEEDIQEQEQRLNELNSKLSELEKSYNDYKAK  591

Query  194  YVRDTKTVTLAVIDEYDLVAQWHEAEKLKVYFERIHREIRNPAGMPNDLFKKLIKDLKEY  253
            Y      +   V  E  +  +  E EKLK+ +ER+  EI    G+  + ++KL ++    
Sbjct  592  YQFLPADLKSLVSLEERIRRRISELEKLKIEYERLKEEITRMKGLKEE-YEKLKEEEDAL  650

Query  254  EHNLKEL  260
             + + EL
Sbjct  651  LNRISEL  657


 Score = 25.8 bits (55),  Expect = 0.003
 Identities = 35/164 (21%), Positives = 72/164 (44%), Gaps = 29/164 (18%)

Query  88   DFTNKEREFNTQRIHAEQYQALKEIFDKLPELQQQFEEVLEQTGRFTFIDIKMGIKALQN  147
            DF  KE+ +N      ++    +E  + +      F+ +L Q        +K  I  ++N
Sbjct  266  DFEEKEKRYNKIETEVKELDENREKINTI----SSFKSILVQID-----SLKSQINVVEN  316

Query  148  DAFDYWMNQMIKAKITSKGLITEEQFKTEQ----EKEIDEEFSQGWGKKQYVRDTKTVTL  203
            D       +  K K+  K  + E++ + E+    +KE++E+      +KQY    K +T 
Sbjct  317  DL------KRKKEKLKRKKELEEKEKQYEEIEKRKKELEEK------EKQYEEIEKRLTY  364

Query  204  AVIDEYDLVAQWHEAEKLK-VYFERIHREIRNPAGMPNDLFKKL  246
             +    ++  Q +E EKL  V  + +  +I++ +   N +  +L
Sbjct  365  VL---KNIERQKNEIEKLNYVDTQDLENKIKDVSDRINQIDNEL  405


 Score = 22.3 bits (46),  Expect = 0.038
 Identities = 40/199 (20%), Positives = 81/199 (41%), Gaps = 44/199 (22%)

Query  104  EQYQALKEIFDKLPELQQQFEEVLEQTGRFTFIDIKMGIKALQNDAFDYWMNQMIKAKIT  163
            +QY+ +++   +L E ++Q+EE+ +   R T++   +  +  + +  +Y   Q ++ KI 
Sbjct  336  KQYEEIEKRKKELEEKEKQYEEIEK---RLTYVLKNIERQKNEIEKLNYVDTQDLENKIK  392

Query  164  SKGLITEEQFKTEQEKEIDEEFSQGWGKKQYVRDTKTVTLAVIDEYDLVA----------  213
                       +++  +ID E      ++  +   K  TL + +  + +           
Sbjct  393  D---------VSDRINQIDNELKGLLDRRGDLNGRKEQTLKIYNNLNSIEDDRCPICGRP  443

Query  214  --QWHEA---EKLKVYFERIHREIRNPAGMPNDLFK--------------KLIKDLKE--  252
                H+A   E++KV    ++++I       N L K              +L K  KE  
Sbjct  444  LDSEHKAKIREEIKVQLLELNKQITALQARINSLIKEREELEATRNKLQLELQKRSKEKG  503

Query  253  -YEHNLKELKNFCIRGNRL  270
             YE  LKEL+      N+L
Sbjct  504  IYEAKLKELQRLEEEKNKL  522


 Score = 19.2 bits (38),  Expect = 0.35
 Identities = 33/175 (19%), Positives = 65/175 (37%), Gaps = 7/175 (4%)

Query  87   VDFTNKEREFNTQRIHAEQYQALKEIFDKLPELQQQFEEVLEQTGRFTFIDIKMGIKALQ  146
            V   N ERE   +     QY      F+++ E++ Q++ +   +G  + ++ K+   AL+
Sbjct  210  VKLENIEREAKEKEDELNQYNT---EFNRIKEIKVQYDIL---SGELSVVNKKIEEIALR  263

Query  147  NDAFDYWMNQMIKAKITSKGLITEEQFKTEQEKEIDEEFSQGWGKKQYVRDTKTVTLAVI  206
               F+    +  K +   K L  E + K            Q    K  +   +       
Sbjct  264  LKDFEEKEKRYNKIETEVKEL-DENREKINTISSFKSILVQIDSLKSQINVVENDLKRKK  322

Query  207  DEYDLVAQWHEAEKLKVYFERIHREIRNPAGMPNDLFKKLIKDLKEYEHNLKELK  261
            ++     +  E EK     E+  +E+        ++ K+L   LK  E    E++
Sbjct  323  EKLKRKKELEEKEKQYEEIEKRKKELEEKEKQYEEIEKRLTYVLKNIERQKNEIE  377


 Score = 19.2 bits (38),  Expect = 0.35
 Identities = 24/159 (15%), Positives = 67/159 (42%), Gaps = 30/159 (19%)

Query  109  LKEIFDKLPELQQQFEEVLEQTGRFT-----FIDIKMGIKALQNDAFDYWMNQMIKAKIT  163
            +K++ D++ ++  + + +L++ G         + I   + ++++D          +  I 
Sbjct  391  IKDVSDRINQIDNELKGLLDRRGDLNGRKEQTLKIYNNLNSIEDD----------RCPIC  440

Query  164  SKGLITEEQFKTEQEKEIDEEFSQGWGKKQYVRDTKTVTLAVIDEYDLVAQWHEAEKLKV  223
             + L +E + K  +E ++           Q +   K +T        L+    E E+L+ 
Sbjct  441  GRPLDSEHKAKIREEIKV-----------QLLELNKQITALQARINSLIK---EREELEA  486

Query  224  YFERIHREIRNPAGMPNDLFKKLIKDLKEYEHNLKELKN  262
               ++  E++  +     +++  +K+L+  E    +L+N
Sbjct  487  TRNKLQLELQKRS-KEKGIYEAKLKELQRLEEEKNKLQN  524


 Score = 18.5 bits (36),  Expect = 0.60
 Identities = 36/178 (20%), Positives = 68/178 (38%), Gaps = 46/178 (26%)

Query  13   EEITSRPNFIDSVHISQNENGVRIKDLVGHYQFKERIKCGIAQCGTKHLKGYIVKLTNGF  72
            E+I  +   ++ ++   +E      D    YQF               L   +  L +  
Sbjct  562  EDIQEQEQRLNELNSKLSELEKSYNDYKAKYQF---------------LPADLKSLVSLE  606

Query  73   EIVIGHVCGRNNFGVDFTNKEREFNTQRIHAEQYQALKE----IFDKLPEL------QQQ  122
            E +   +       +++   + E    +   E+Y+ LKE    + +++ EL       +Q
Sbjct  607  ERIRRRISELEKLKIEYERLKEEITRMKGLKEEYEKLKEEEDALLNRISELGYSEKRYKQ  666

Query  123  FEEVLEQTGRFTFIDIKMGIKALQNDAFDYWMNQMIKAKITSKGLITEEQFKTEQEKE  180
             EE++++  +     I  GI+A              K KI  KG + EE+ K  +EKE
Sbjct  667  LEEIIDKLSK-----ILSGIEA-------------DKGKI--KGSL-EEKIKNIEEKE  703


 Score = 15.0 bits (27),  Expect = 7.2
 Identities = 8/21 (38%), Positives = 12/21 (57%), Gaps = 4/21 (19%)

Query  225  FERIHREIRNPAGMPNDLFKK  245
            F+R +R+I    G   +L KK
Sbjct  48   FKRTNRDI----GKNEELIKK  64



Lambda      K        H        a         alpha
   0.323    0.139    0.410    0.792     4.96 

Gapped
Lambda      K        H        a         alpha    sigma
   0.267   0.0410    0.140     1.90     42.6     43.6 

Effective search space used: 226366


  Database: 464b597d11387c910ce144a513523f91.SwissProt.fasta
    Posted date:  May 20, 2024  11:58 AM
  Number of letters in database: 886
  Number of sequences in database:  1



Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 11
Window for multiple hits: 40