BLASTP 2.12.0+
Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics: Alejandro A. Schaffer,
L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri
I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids
Res. 29:2994-3005.
Database: 2495f605fc4f663f9fd3af8618158786.SwissProt.fasta
3 sequences; 746 total letters
Query= ACIAD2266
Length=153
Score E
Sequences producing significant alignments: (Bits) Value
P25338 2-hydroxy-palmitic acid dioxygenase MPO1 [Saccharomyces ce... 64.3 9e-18
O13737 2-hydroxy-palmitic acid dioxygenase mpo1 [Schizosaccharomy... 55.5 3e-14
Q08DE2 Transmembrane protein 185B [Bos taurus] 33.1 3e-06
>P25338 2-hydroxy-palmitic acid dioxygenase MPO1 [Saccharomyces
cerevisiae (strain ATCC 204508 / S288c)]
Length=174
Score = 64.3 bits (155), Expect = 9e-18
Identities = 47/146 (32%), Positives = 76/146 (52%), Gaps = 8/146 (5%)
Query 14 YSQSHQNPTNKKIHWVCVPAILFSIIGLIY----HFSALLTALLVVLSLVFYARLDLVLA 69
Y H NP N IH + VP ILFS +++ + S LTA+L VL +FY L L
Sbjct 15 YKFYHHNPKNVLIHSIFVPTILFSGSCMLHRVKIYQSISLTAVLSVLFSIFYCLLYLPTG 74
Query 70 VAMSALIVV--MAWLIHVLPVGVGFYIGVFVVAWIGQFYGHKI-EGKKPSFFKDL-QFLL 125
+ L+++ +A + H + + +G+F + WI QF GH + E ++P+ +L Q L+
Sbjct 75 LLAGVLLLLLNLALIDHRVDLTFKQELGLFTIGWIFQFVGHGVFEKRRPALIDNLVQSLV 134
Query 126 IGPIWCMDAYLEKILPKWRSKQKINH 151
+ P + M +L K+ R K + H
Sbjct 135 LAPYFIMFEFLFKLGFMPRLKATLEH 160
>O13737 2-hydroxy-palmitic acid dioxygenase mpo1 [Schizosaccharomyces
pombe (strain 972 / ATCC 24843)]
Length=222
Score = 55.5 bits (132), Expect = 3e-14
Identities = 48/176 (27%), Positives = 81/176 (46%), Gaps = 30/176 (17%)
Query 4 MKTITEWFDEYSQSHQNPTNKKIHWVCVPAILFSIIGLIYHFSALL--------TALLVV 55
M ++ + Y+ H NP N KIH VC+P +L + + L+++F L A LV
Sbjct 1 MTYLSRSYSFYAAYHSNPVNIKIHQVCIPLLLLTALVLLHNFVITLINSKLQINVAHLVG 60
Query 56 LSL-VFYARLDLVLAVAMSALIVVMAWLIHVLPVGVGFYIG----------VFVVAWIGQ 104
L+ +FY LD + + S ++ + + ++LP + V V+ WI Q
Sbjct 61 LAYQIFYVTLDPLDGLLYSPVLYLFS---YILPSKLFTIFSRSLVNRSAAVVHVICWILQ 117
Query 105 FYGHKI-EGKKPSFFKDL-QFLLIGPIWC------MDAYLEKILPKWRSKQKINHV 152
F GH + E +KP+ +L Q L I P++ Y ++ K R+ K+ V
Sbjct 118 FIGHGVFEKRKPALLDNLIQSLFIAPLFAFLETGPFVGYYPSVVSKIRANIKLKDV 173
>Q08DE2 Transmembrane protein 185B [Bos taurus]
Length=350
Score = 33.1 bits (74), Expect = 3e-06
Identities = 21/91 (23%), Positives = 41/91 (45%), Gaps = 16/91 (18%)
Query 26 IHWVCVPAILFSIIGLIYHFSALLTALLVVLSLVFYARLDLVLAVAMSALIVVMAWLIHV 85
IHW + ++F + ++ F L+ +V SL+F LD+V + + + + W+ V
Sbjct 164 IHWPWL--VVFVPLWILMSFLCLVVLYYIVWSLLFLRSLDVVAEQRRTHVTMAICWITIV 221
Query 86 LPV--------------GVGFYIGVFVVAWI 102
+P+ + YI +FV W+
Sbjct 222 VPLLIFEVLLVHRLDGHNMFSYISIFVPLWL 252
Score = 16.5 bits (31), Expect = 0.86
Identities = 12/41 (29%), Positives = 21/41 (51%), Gaps = 1/41 (2%)
Query 55 VLSLVFYA-RLDLVLAVAMSALIVVMAWLIHVLPVGVGFYI 94
+L +F A RLD ++ + V + L+ L + V +YI
Sbjct 150 ILQFIFIALRLDRIIHWPWLVVFVPLWILMSFLCLVVLYYI 190
Lambda K H a alpha
0.334 0.145 0.486 0.792 4.96
Gapped
Lambda K H a alpha sigma
0.267 0.0410 0.140 1.90 42.6 43.6
Effective search space used: 83790
Database: 2495f605fc4f663f9fd3af8618158786.SwissProt.fasta
Posted date: Jun 2, 2024 11:46 AM
Number of letters in database: 746
Number of sequences in database: 3
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 11
Window for multiple hits: 40
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Data ...
