BLASTP 2.12.0+
Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics: Alejandro A. Schaffer,
L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri
I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids
Res. 29:2994-3005.
Database: 0a37056b8181789e8e102f53da6c5468.SwissProt.fasta
13 sequences; 2,866 total letters
Query= ACIAD2674
Length=182
Score E
Sequences producing significant alignments: (Bits) Value
Q6Q271 p-hydroxyphenylacetate 3-hydroxylase, reductase component ... 135 8e-43
P54990 FMN reductase (NADH) NtaB [Aminobacter aminovorans] 135 1e-42
Q6F9F5 Flavin-dependent trigonelline monooxygenase, reductase com... 120 3e-37
Q92ZM6 Probable flavin reductase [Rhizobium meliloti (strain 1021)] 113 2e-34
Q0S808 Flavin-dependent monooxygenase, reductase subunit HsaB [Rh... 105 1e-32
P9WND8 Flavin-dependent monooxygenase, reductase subunit HsaB [My... 101 7e-31
P9WND9 Flavin-dependent monooxygenase, reductase subunit HsaB [My... 101 7e-31
Q8RQP9 NADH-dependent flavin reductase [Rhodococcus sp] 100 1e-30
M4YFG7 Flavin reductase [Pseudomonas putida] 98.6 5e-30
Q6F4M9 4-nitrophenol 4-monooxygenase/4-nitrocatechol 2-monooxygen... 97.1 3e-29
Q02058 Actinorhodin polyketide dimerase [Streptomyces coelicolor ... 94.7 2e-28
Q8GME2 NADH-dependent FAD reductase [Streptomyces globisporus] 89.0 4e-26
Q5SJP7 4-hydroxyphenylacetate 3-monooxygenase, reductase componen... 77.4 4e-22
>Q6Q271 p-hydroxyphenylacetate 3-hydroxylase, reductase component
[Acinetobacter baumannii]
Length=315
Score = 135 bits (340), Expect = 8e-43
Identities = 67/160 (42%), Positives = 95/160 (59%), Gaps = 0/160 (0%)
Query 21 NPREIRNLLGQFATGVTVITTRGRDGRKIGMTANSFSSLSLDPPLILWSLSKTAPSLPDF 80
+P R LG FATGVT++T + G ++G+TANSF+S+SLDP L+LWS+ K + S F
Sbjct 15 DPMAFRRALGNFATGVTIMTAQTSSGERVGVTANSFNSVSLDPALVLWSIDKKSSSYRIF 74
Query 81 TEAEYFAIHMLAQEHHSLSGHFARGSEDKFASIAHRECERGLPLLEDVLATLVCKNINQY 140
EA +F +++L+ LS FAR SEDKFA+I +PL ++ A C+ N
Sbjct 75 EEATHFGVNILSAAQIELSNRFARRSEDKFANIEFDLGVGNIPLFKNCSAAFECERYNIV 134
Query 141 EGGDHLIFIGQIEHYQQRIGEPLVFHAGKYRIAAEHPELS 180
EGGDH I IG++ + PL++H G Y HP L+
Sbjct 135 EGGDHWIIIGRVVKFHDHGRSPLLYHQGAYSAVLPHPSLN 174
Score = 15.4 bits (28), Expect = 9.5
Identities = 5/9 (56%), Positives = 6/9 (67%), Gaps = 0/9 (0%)
Query 170 YRIAAEHPE 178
Y+IA H E
Sbjct 283 YKIAESHQE 291
>P54990 FMN reductase (NADH) NtaB [Aminobacter aminovorans]
Length=322
Score = 135 bits (339), Expect = 1e-42
Identities = 69/160 (43%), Positives = 99/160 (62%), Gaps = 1/160 (1%)
Query 19 TENPREIRNLLGQFATGVTVITTRGRDGRKIGMTANSFSSLSLDPPLILWSLSKTAPSLP 78
T +P+ R LG F TGVT++T G DG G+TANSF+S+SLDPPL+LWS+ T+ S
Sbjct 15 TSDPKGFRRALGTFPTGVTIVTAPGVDG-PAGVTANSFASVSLDPPLVLWSIGHTSRSHS 73
Query 79 DFTEAEYFAIHMLAQEHHSLSGHFARGSEDKFASIAHRECERGLPLLEDVLATLVCKNIN 138
F ++ FAI++LA + +S FA GS DKF+ + G PL+++ LA C
Sbjct 74 KFQQSATFAINILADDQVGVSQVFAGGSADKFSLVDWHTGRTGAPLIDNALAYFDCVCEA 133
Query 139 QYEGGDHLIFIGQIEHYQQRIGEPLVFHAGKYRIAAEHPE 178
++EGGDH I IG++ + + G PL F G+Y + +HPE
Sbjct 134 RHEGGDHTIMIGRVVDFGRAEGSPLAFSQGRYGVTLDHPE 173
>Q6F9F5 Flavin-dependent trigonelline monooxygenase, reductase
component [Acinetobacter baylyi (strain ATCC 33305 / BD413
/ ADP1)]
Length=310
Score = 120 bits (302), Expect = 3e-37
Identities = 62/148 (42%), Positives = 93/148 (63%), Gaps = 0/148 (0%)
Query 23 REIRNLLGQFATGVTVITTRGRDGRKIGMTANSFSSLSLDPPLILWSLSKTAPSLPDFTE 82
RE+R+ G F TGVTV+TT DG +G TANSF+S+SLDP L+L S++KT+ + +F +
Sbjct 11 RELRDAFGSFMTGVTVVTTCKDDGTPLGFTANSFASVSLDPALLLVSIAKTSSNYHNFAD 70
Query 83 AEYFAIHMLAQEHHSLSGHFARGSEDKFASIAHRECERGLPLLEDVLATLVCKNINQYEG 142
A +FAI++LA+E +S FAR S+D+FA + + E PL++ V A C +
Sbjct 71 ASHFAINILAEEQKDVSNIFARPSDDRFAQLVWAKSEYQNPLIDGVSAWFDCTTYQVVDA 130
Query 143 GDHLIFIGQIEHYQQRIGEPLVFHAGKY 170
GDH I IG++E++ L ++ G Y
Sbjct 131 GDHAILIGKVENFTSAGFAGLGYYRGAY 158
>Q92ZM6 Probable flavin reductase [Rhizobium meliloti (strain
1021)]
Length=311
Score = 113 bits (283), Expect = 2e-34
Identities = 61/150 (41%), Positives = 89/150 (59%), Gaps = 0/150 (0%)
Query 21 NPREIRNLLGQFATGVTVITTRGRDGRKIGMTANSFSSLSLDPPLILWSLSKTAPSLPDF 80
+PR +R+ G FATGVTV+T G+ IG TANSF+S+SLDPPL+L L+K++ +
Sbjct 7 DPRALRDAFGAFATGVTVVTASDAAGKPIGFTANSFTSVSLDPPLLLVCLAKSSRNYESM 66
Query 81 TEAEYFAIHMLAQEHHSLSGHFARGSEDKFASIAHRECERGLPLLEDVLATLVCKNINQY 140
T A FAI++L++ +S FAR ED+FA++ R G P+ DV A C +
Sbjct 67 TSAGRFAINVLSETQKDVSNTFARPVEDRFAAVDWRLGRDGCPIFSDVAAWFECSMQDII 126
Query 141 EGGDHLIFIGQIEHYQQRIGEPLVFHAGKY 170
E GDH+I IG++ ++ L + G Y
Sbjct 127 EAGDHVIIIGRVTAFENSGLNGLGYARGGY 156
Score = 16.9 bits (32), Expect = 3.0
Identities = 11/29 (38%), Positives = 14/29 (48%), Gaps = 3/29 (10%)
Query 89 HMLAQEHHSLSGHFARGSE---DKFASIA 114
H LA + G F R +E KF+S A
Sbjct 244 HALASDGAPRQGRFLRPAELAAAKFSSSA 272
>Q0S808 Flavin-dependent monooxygenase, reductase subunit HsaB
[Rhodococcus jostii (strain RHA1)]
Length=195
Score = 105 bits (263), Expect = 1e-32
Identities = 58/151 (38%), Positives = 86/151 (57%), Gaps = 2/151 (1%)
Query 21 NPREIRNLLGQFATGVTVITTRGRDGRKIGMTANSFSSLSLDPPLILWSLSKTAPSLPDF 80
+PR R +LGQF TGVT+ITT DG +G SF++LSL+PPL+L+ +KT+ S
Sbjct 16 DPRRFRTVLGQFCTGVTIITTID-DGVPVGFACQSFAALSLEPPLVLFCPTKTSRSWAAI 74
Query 81 TEAEYFAIHMLAQEHHSLSGHFARGSEDKFASIAHRECERGLPLLEDVLATLVCKNINQY 140
+ F +++LA+E S F DKFA I E G P+L LA + C + +
Sbjct 75 ERSGIFCVNVLAEEQQSTCARFGSRDPDKFAGIDWTESPLGSPILTGSLAHIDCSLESVH 134
Query 141 EGGDHLIFIGQIEHYQQ-RIGEPLVFHAGKY 170
+GGDH + G++ + R PL+F+ G+Y
Sbjct 135 DGGDHWVAFGRVSSLSEIREERPLLFYRGQY 165
>P9WND8 Flavin-dependent monooxygenase, reductase subunit HsaB
[Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh)]
Length=187
Score = 101 bits (251), Expect = 7e-31
Identities = 55/153 (36%), Positives = 85/153 (56%), Gaps = 4/153 (3%)
Query 21 NPREIRNLLGQFATGVTVITTRGRDGRKIGMTANSFSSLSLDPPLILWSLSKTAPSLPDF 80
+PR R++LGQF TG+TVITT D +G SF++LSL+PPL+L+ +K + S
Sbjct 6 DPRTFRSVLGQFCTGITVITTV-HDDVPVGFACQSFAALSLEPPLVLFCPTKVSRSWQAI 64
Query 81 TEAEYFAIHMLAQEHHSLSGHFARGSEDKFASIAHRECERGLPLLEDVLATLVCKNINQY 140
+ F +++L ++ +S F DKFA I R E G P++E LA + C + +
Sbjct 65 EASGRFCVNVLTEKQKDVSARFGSKEPDKFAGIDWRPSELGSPIIEGSLAYIDCTVASVH 124
Query 141 EGGDHLIFIGQIEHYQQRIG---EPLVFHAGKY 170
+GGDH + G +E + PL+F+ G Y
Sbjct 125 DGGDHFVVFGAVESLSEVPAVKPRPLLFYRGDY 157
>P9WND9 Flavin-dependent monooxygenase, reductase subunit HsaB
[Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)]
Length=187
Score = 101 bits (251), Expect = 7e-31
Identities = 55/153 (36%), Positives = 85/153 (56%), Gaps = 4/153 (3%)
Query 21 NPREIRNLLGQFATGVTVITTRGRDGRKIGMTANSFSSLSLDPPLILWSLSKTAPSLPDF 80
+PR R++LGQF TG+TVITT D +G SF++LSL+PPL+L+ +K + S
Sbjct 6 DPRTFRSVLGQFCTGITVITTV-HDDVPVGFACQSFAALSLEPPLVLFCPTKVSRSWQAI 64
Query 81 TEAEYFAIHMLAQEHHSLSGHFARGSEDKFASIAHRECERGLPLLEDVLATLVCKNINQY 140
+ F +++L ++ +S F DKFA I R E G P++E LA + C + +
Sbjct 65 EASGRFCVNVLTEKQKDVSARFGSKEPDKFAGIDWRPSELGSPIIEGSLAYIDCTVASVH 124
Query 141 EGGDHLIFIGQIEHYQQRIG---EPLVFHAGKY 170
+GGDH + G +E + PL+F+ G Y
Sbjct 125 DGGDHFVVFGAVESLSEVPAVKPRPLLFYRGDY 157
>Q8RQP9 NADH-dependent flavin reductase [Rhodococcus sp]
Length=176
Score = 100 bits (248), Expect = 1e-30
Identities = 57/152 (38%), Positives = 83/152 (55%), Gaps = 4/152 (3%)
Query 22 PREIRNLLGQFATGVTVITTRGRDGRKI--GMTANSFSSLSLDPPLILWSLSKTAPSLPD 79
P +R LG+FA+GVT++TT + GMTAN+F+S+SLDPPL+L S+S A
Sbjct 14 PLHLRKALGRFASGVTIVTTAECEDEDSVHGMTANAFTSVSLDPPLVLVSISTRAKMDTK 73
Query 80 FTEAEYFAIHMLAQEHHSLSGHFARGSEDKFASIAHRECERGLPLLEDVLATLVCKNINQ 139
E + I +LA + +S HFA + + RG+PLLE L L C +
Sbjct 74 IRETGTYGISILAGDQEPVSLHFAGAAHEP--DRVRFVWRRGVPLLEGALVHLACTVVAS 131
Query 140 YEGGDHLIFIGQIEHYQQRIGEPLVFHAGKYR 171
+ GDH + +G++E G PLVF+ G +R
Sbjct 132 HPAGDHTLHVGRVEQLWYDDGHPLVFYTGSFR 163
>M4YFG7 Flavin reductase [Pseudomonas putida]
Length=170
Score = 98.6 bits (244), Expect = 5e-30
Identities = 51/151 (34%), Positives = 84/151 (56%), Gaps = 1/151 (1%)
Query 21 NPREIRNLLGQFATGVTVITTRGRDGRKIGMTANSFSSLSLDPPLILWSLSKTAPSLPDF 80
+P+ R +LGQ+ TGV +T DG+ GM SF+S+SL+PPL+ + +++ S P
Sbjct 14 DPQWFRAVLGQYPTGVCAVTAMDPDGKMSGMAVGSFTSVSLNPPLVAFLPDRSSTSWPKI 73
Query 81 TEAEYFAIHMLAQEHHSLSGHFARGSEDKFASIAHRECERGLPLLEDVLATLVCKNINQY 140
A F +++L+ + + FA EDKF+ + +R + G P++E V+A + C +
Sbjct 74 ERAGKFCVNVLSDQQLGVCKRFASKDEDKFSGLVYRLSDNGSPIIEGVVAWIDCDLHSVQ 133
Query 141 EGGDHLIFIGQIEHYQ-QRIGEPLVFHAGKY 170
E GDH I IG + Q + L+F+ G Y
Sbjct 134 EAGDHYIVIGSVRELQVESEDSALLFYRGGY 164
>Q6F4M9 4-nitrophenol 4-monooxygenase/4-nitrocatechol 2-monooxygenase,
reductase component [Rhodococcus opacus]
Length=185
Score = 97.1 bits (240), Expect = 3e-29
Identities = 54/157 (34%), Positives = 89/157 (57%), Gaps = 5/157 (3%)
Query 14 LKPIDTENPREIRNLLGQFATGVTVITTRGRDGRKIGMTANSFSSLSLDPPLILWSLSKT 73
L+P+D E RN++G FA+GVT++T DG G T ++ +SL PP++L L++
Sbjct 11 LQPLDKA---EFRNVVGHFASGVTIVTA-AHDGVPYGATISAVTSLCDTPPMVLVCLNQK 66
Query 74 APSLPDFTEAEYFAIHMLAQEHHSLSGHFARGSEDKFASIAHRECERGLPLLEDVLATLV 133
+ +A +F I++L ++ SL+ FA DKFA +A + G P L + LA L
Sbjct 67 LGTHAAIRKARHFTINILGEDQASLAHTFATPGADKFADVAVHHRQHG-PRLAEALAYLT 125
Query 134 CKNINQYEGGDHLIFIGQIEHYQQRIGEPLVFHAGKY 170
C+ ++ EGG H IF+ ++ Q G PL ++ G++
Sbjct 126 CRVVDDLEGGTHRIFVAEVVEAQAGTGNPLSYYRGRF 162
>Q02058 Actinorhodin polyketide dimerase [Streptomyces coelicolor
(strain ATCC BAA-471 / A3(2) / M145)]
Length=177
Score = 94.7 bits (234), Expect = 2e-28
Identities = 51/143 (36%), Positives = 82/143 (57%), Gaps = 3/143 (2%)
Query 25 IRNLLGQFATGVTVITTRGRDGRKIGMTANSFSSLSLDPPLILWSLSKTAPSLPDFTEAE 84
+R+ + + GV ++T R G G TA+SF S+S++PPL L L++TA S P F
Sbjct 8 LRDAMARVPAGVALVTAHDRGGVPHGFTASSFVSVSMEPPLALVCLARTANSFPVFDSCG 67
Query 85 YFAIHMLAQEHHSLSGHFARGSEDKFASIAHRECERGLPLLEDVLATLVCKNINQYEGGD 144
FA+ +L ++H L+ FAR S DKFA RG +L+ +A + C +Y GD
Sbjct 68 EFAVSVLREDHTDLAMRFARKSADKFAGGEFVRTARGATVLDGAVAVVECTVHERYPAGD 127
Query 145 HLIFIGQIE--HYQQRIGEPLVF 165
H+I +G+++ H +++ G P V+
Sbjct 128 HIILLGEVQSVHVEEK-GVPAVY 149
Score = 16.2 bits (30), Expect = 5.0
Identities = 9/21 (43%), Positives = 10/21 (48%), Gaps = 0/21 (0%)
Query 23 REIRNLLGQFATGVTVITTRG 43
R R +FA G V T RG
Sbjct 84 RFARKSADKFAGGEFVRTARG 104
>Q8GME2 NADH-dependent FAD reductase [Streptomyces globisporus]
Length=182
Score = 89.0 bits (219), Expect = 4e-26
Identities = 59/161 (37%), Positives = 86/161 (53%), Gaps = 8/161 (5%)
Query 17 IDTENPREIRNLLGQFATGVTVITTRGRDGRKIGMTANSFSSLSLDPPLILWSLSKTAPS 76
+D ++ ++R + G F TGVTV+T G + R GMTANSF+S+SL PPL+L + K A
Sbjct 12 VDPKDRVQLRRVFGDFPTGVTVVTVGGSEPR--GMTANSFTSVSLSPPLVLICVGKDAVM 69
Query 77 LPDFTEAEYFAIHMLAQEHHSLSGHFARGSE----DKFASIAH-RECERGLPLLEDVLAT 131
T FA+ +L + HFA S D+F ++ E G PL+ +A
Sbjct 70 HQRLTALPTFAVSVLEAGQEKAARHFADHSRPPGVDQFDTVDWVLGEESGAPLIAGAVAH 129
Query 132 LVCKNINQYEGGDHLIFIGQ-IEHYQQRIGEPLVFHAGKYR 171
L C YEGGDH IF+G+ I + E ++F G++R
Sbjct 130 LECAIHRLYEGGDHTIFLGEVITATRWPAREGMLFSGGRFR 170
>Q5SJP7 4-hydroxyphenylacetate 3-monooxygenase, reductase component
[Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8)]
Length=149
Score = 77.4 bits (189), Expect = 4e-22
Identities = 54/148 (36%), Positives = 79/148 (53%), Gaps = 12/148 (8%)
Query 26 RNLLGQFATGVTVITTR-GRDGRKIGMTANSFSSLSLDPPLILWSLSKTAPSLPDFTEAE 84
+ L +FA+GVTV+ R G + R GMTA +F SLSL+PPL+ ++S+ A LP A
Sbjct 6 KEALARFASGVTVVAARLGEEER--GMTATAFMSLSLEPPLVALAVSERAKLLPVLEGAG 63
Query 85 YFAIHMLAQEHHSLSGHFARGSEDKFASIAHRECERGLPLLEDVLATLVCKNINQYEGGD 144
F + +L + ++S HFA ++ A E G ++ LA L C+ Y GGD
Sbjct 64 AFTVSLLREGQEAVSEHFAGRPKEGIA------LEEG--RVKGALAVLRCRLHALYPGGD 115
Query 145 HLIFIGQIEHYQ-QRIGEPLVFHAGKYR 171
H I +G +E + G PLV+ YR
Sbjct 116 HRIVVGLVEEVELGEEGPPLVYFQRGYR 143
Lambda K H a alpha
0.322 0.138 0.414 0.792 4.96
Gapped
Lambda K H a alpha sigma
0.267 0.0410 0.140 1.90 42.6 43.6
Effective search space used: 345825
Database: 0a37056b8181789e8e102f53da6c5468.SwissProt.fasta
Posted date: May 20, 2024 5:48 PM
Number of letters in database: 2,866
Number of sequences in database: 13
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 11
Window for multiple hits: 40
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Data ...
