BLASTP 2.12.0+
Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics: Alejandro A. Schaffer,
L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri
I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids
Res. 29:2994-3005.
Database: c43a4be951bcfb14269d246c478e6cdb.SwissProt.fasta
13 sequences; 3,459 total letters
Query= ACIAD2723
Length=202
Score E
Sequences producing significant alignments: (Bits) Value
P21367 Probable hydrolase YcaC [Escherichia coli (strain K12)] 129 5e-41
G4YEI5 Secreted isochorismatase effector Isc1 [Phytophthora sojae... 64.3 2e-16
Q5U3Z3 Isochorismatase domain-containing protein 2 [Rattus norveg... 62.8 9e-16
Q54RC7 Isochorismatase family protein 2B [Dictyostelium discoideum] 58.5 3e-14
P85094 Isochorismatase domain-containing protein 2A [Mus musculus] 57.8 5e-14
Q9DCC7 Isochorismatase domain-containing protein 2B [Mus musculus] 57.8 6e-14
Q5PQ71 Isochorismatase domain-containing protein 2 [Xenopus laevis] 54.7 7e-13
A0JME6 Isochorismatase domain-containing protein 2 [Danio rerio] 53.5 2e-12
Q32KX0 Isochorismatase domain-containing protein 2 [Bos taurus] 52.8 3e-12
Q54RF0 Isochorismatase family protein 2A [Dictyostelium discoideum] 52.4 4e-12
Q96AB3 Isochorismatase domain-containing protein 2 [Homo sapiens] 49.7 4e-11
F4JLP5 Dihydrolipoyl dehydrogenase 2, chloroplastic [Arabidopsis ... 38.9 4e-07
A8MS68 Dihydrolipoyl dehydrogenase 1, chloroplastic [Arabidopsis ... 37.4 1e-06
>P21367 Probable hydrolase YcaC [Escherichia coli (strain K12)]
Length=208
Score = 129 bits (323), Expect = 5e-41
Identities = 63/172 (37%), Positives = 104/172 (60%), Gaps = 2/172 (1%)
Query 5 RFTPENSALLLIDHQVGTMQLIKNIDKELAAKQSIALAKMAKILGIPVVITSSQEDNAQG 64
R ++A+LL+DHQ G + L+++I+ + +AL +AK +P ++T+S E G
Sbjct 7 RLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALGDLAKYFNLPTILTTSFETGPNG 66
Query 65 PILPEIAETLPEAYEARVKRLGVVNAWADQEFHDAVVATGRKNLIMAGVTTDVCLIFPAI 124
P++PE+ P+ + R G +NAW +++F AV ATG+K LI+AGV T+VC+ FPA+
Sbjct 67 PLVPELKAQFPDT--PYIARPGNINAWDNEDFVKAVKATGKKQLIIAGVVTEVCVAFPAL 124
Query 125 DAVAEGFAVQAVIDASGSPSDLSEEFSRQRMHDAGVVLTATNTLMAEIAQDW 176
A+ EGF V V DASG+ ++++ + R+ AG L + E+ +DW
Sbjct 125 SAIEEGFDVFVVTDASGTFNEITRHSAWDRLSQAGAQLMTWFGVACELHRDW 176
>G4YEI5 Secreted isochorismatase effector Isc1 [Phytophthora sojae
(strain P6497)]
Length=210
Score = 64.3 bits (155), Expect = 2e-16
Identities = 44/185 (24%), Positives = 91/185 (49%), Gaps = 4/185 (2%)
Query 5 RFTPENSALLLIDHQVGTMQLIKNIDKELAAKQSIALAKMAKILGIPVVITSSQEDNAQG 64
R P +S L + D Q L + + ++ A AK+L +PVV+T+ Q + G
Sbjct 13 RLIPHSSVLFVCDVQEVFRGLTFQLPTVIHGTNTMVSA--AKLLNVPVVVTT-QYGSRLG 69
Query 65 PILPEIAETLPEAYEARV-KRLGVVNAWADQEFHDAVVATGRKNLIMAGVTTDVCLIFPA 123
+ EI++ L +A + +V ++ + E H RK++++ G+ T VC++
Sbjct 70 STVSEISKNLEDAPDVKVFDKMKFSMLVPEVERHLTTNMPQRKSVLLCGIETHVCVLQTC 129
Query 124 IDAVAEGFAVQAVIDASGSPSDLSEEFSRQRMHDAGVVLTATNTLMAEIAQDWSSPTGQQ 183
+D + +G+ V V DA S + + + +R+ +G +T+ + + ++A D +P +
Sbjct 130 LDLLDKGYDVHVVSDAVSSSTSYNRSMALERIRQSGAYITSVESAIFQLANDAGNPEFKS 189
Query 184 LIGLL 188
+ L+
Sbjct 190 ISKLI 194
>Q5U3Z3 Isochorismatase domain-containing protein 2 [Rattus norvegicus]
Length=210
Score = 62.8 bits (151), Expect = 9e-16
Identities = 54/201 (27%), Positives = 97/201 (48%), Gaps = 26/201 (13%)
Query 5 RFTPENSALLLIDHQVGTMQLIKNIDKELAAKQSIALA----KMAKILGIPVVITSSQED 60
R PE+S L L D Q K D+ L Q +++A K+A+IL +PV++T
Sbjct 10 RIFPESSILFLCDMQE------KLRDRVLYFPQIVSMAARMLKVARILDVPVLLTEHYPQ 63
Query 61 NAQGPILPEIA----ETLPEAYEARVKRLGVVNAWADQEFHDAVVATGRKNLIMAGVTTD 116
GP +PE+ T+ + + V L Q+ D + K++++ G+ T
Sbjct 64 GL-GPTVPELGAQGVRTMSKTSFSMVPPL--------QQELDKLPQL--KSVLLCGIETQ 112
Query 117 VCLIFPAIDAVAEGFAVQAVIDASGSPSDLSEEFSRQRMHDAGVVLTATNTLMAEIAQDW 176
VC++ A+D + G V +DA S S+++ + RM +GV L+ + L ++ +D
Sbjct 113 VCILNTALDLLDRGLQVHVAVDACSSQSEMNRLVALARMQHSGVFLSTSEALTLQLIKDA 172
Query 177 SSPTGQQLIGLLFTDVFPALG 197
+ P +++ +L V P +G
Sbjct 173 AHPQFKEIQKILKEPV-PEIG 192
>Q54RC7 Isochorismatase family protein 2B [Dictyostelium discoideum]
Length=214
Score = 58.5 bits (140), Expect = 3e-14
Identities = 27/83 (33%), Positives = 49/83 (59%), Gaps = 0/83 (0%)
Query 106 KNLIMAGVTTDVCLIFPAIDAVAEGFAVQAVIDASGSPSDLSEEFSRQRMHDAGVVLTAT 165
+ +I+AG T VC+I A+D + EG+ V + DA+ S L S +R+ +GV LT T
Sbjct 106 RTVILAGFETHVCIIQTALDLIREGYTVHVITDATASIDSLEYTASLKRLKQSGVYLTTT 165
Query 166 NTLMAEIAQDWSSPTGQQLIGLL 188
++ ++ +D ++P ++I L+
Sbjct 166 EAVLFQLLRDDANPKSSKIIDLI 188
>P85094 Isochorismatase domain-containing protein 2A [Mus musculus]
Length=206
Score = 57.8 bits (138), Expect = 5e-14
Identities = 41/181 (23%), Positives = 85/181 (47%), Gaps = 11/181 (6%)
Query 5 RFTPENSALLLIDHQVGTMQLIKNIDKELAAKQSIALAKMAKILGIPVVITSSQEDNAQG 64
R PE+S L L D Q I + ++ + + K+A++L +P+++T Q G
Sbjct 10 RILPESSILFLCDLQEKFRPSIAYFPQIVSV--AARMLKVARLLDVPILLTE-QYPEGLG 66
Query 65 PILPEI-AETLPEAYEARVKRLGVVNAWADQEFHDAVVATGRKNLIMAGVTTDVCLIFPA 123
P +PE+ A+ + + + + D + +++++ G+ T C++ A
Sbjct 67 PTVPELGAQGIRPVSKTCFSMVPALQKELDGR-------SQLQSVLLCGIETQACILNTA 119
Query 124 IDAVAEGFAVQAVIDASGSPSDLSEEFSRQRMHDAGVVLTATNTLMAEIAQDWSSPTGQQ 183
+D + G V V+DA S S + + RM +G L + +L+ ++ +D S P ++
Sbjct 120 LDLLHRGLQVHVVVDACSSRSQVDRLVALARMRQSGAFLATSESLILQLVRDASHPQFKE 179
Query 184 L 184
+
Sbjct 180 I 180
>Q9DCC7 Isochorismatase domain-containing protein 2B [Mus musculus]
Length=210
Score = 57.8 bits (138), Expect = 6e-14
Identities = 43/185 (23%), Positives = 89/185 (48%), Gaps = 11/185 (6%)
Query 5 RFTPENSALLLIDHQVGTMQLIKNIDKELAAKQSIALAKMAKILGIPVVITSSQEDNAQG 64
R PE+S L L D Q I + ++ ++ + K+A++L IPV++T G
Sbjct 10 RIFPESSILFLCDMQEKLRDRILYFPQIVS--KAARMLKVAQMLEIPVLLTEHYPQGL-G 66
Query 65 PILPEI-AETLPEAYEARVKRLGVVNAWADQEFHDAVVATGRKNLIMAGVTTDVCLIFPA 123
P +PE+ A+ L + + + D+ +++++ G+ T C++ A
Sbjct 67 PTVPELGAQGLRTMSKTSFSMVPPLQQELDK-------LPQLQSVLLCGIETQGCILHTA 119
Query 124 IDAVAEGFAVQAVIDASGSPSDLSEEFSRQRMHDAGVVLTATNTLMAEIAQDWSSPTGQQ 183
+D + G V +DA S S+++ + RM +GV L+ + L+ ++ +D + P ++
Sbjct 120 LDLLDRGLQVHVAVDACSSQSEMNRLVALARMQQSGVFLSTSEVLILQLVKDAAHPQFKE 179
Query 184 LIGLL 188
+ +L
Sbjct 180 IQKIL 184
>Q5PQ71 Isochorismatase domain-containing protein 2 [Xenopus laevis]
Length=205
Score = 54.7 bits (130), Expect = 7e-13
Identities = 34/139 (24%), Positives = 65/139 (47%), Gaps = 6/139 (4%)
Query 41 LAKMAKILGIPVVITSSQEDNAQGPILPEIAETLPEAYEARVKRLGVVNAWADQEFHDAV 100
+ + AK L +PV+IT Q GP +PE+ + Y ++ ++E
Sbjct 43 MLQAAKKLEMPVIITE-QHPKGLGPTVPELGADDLKKYTKT--SFSMLTPEVEEELQSI- 98
Query 101 VATGRKNLIMAGVTTDVCLIFPAIDAVAEGFAVQAVIDASGSPSDLSEEFSRQRMHDAGV 160
+++++ G+ T C++ A+D + +G+ V V DA S S + F+ RM +G
Sbjct 99 --PDLRSIVLCGIETQACIMSTALDLLDKGYDVHVVADACSSRSQVDRLFALSRMRQSGA 156
Query 161 VLTATNTLMAEIAQDWSSP 179
LT + ++ ++ D P
Sbjct 157 FLTTSEGVLLQLLGDAKHP 175
>A0JME6 Isochorismatase domain-containing protein 2 [Danio rerio]
Length=197
Score = 53.5 bits (127), Expect = 2e-12
Identities = 46/186 (25%), Positives = 79/186 (42%), Gaps = 12/186 (6%)
Query 5 RFTPENSALLLIDHQVGTMQLIKNIDKELAAKQSIALAKMAKILGIPVVITSSQEDNAQG 64
R + + S LLL D Q I ++ + L + +IL IP ++T Q G
Sbjct 6 RLSTKGSVLLLCDMQEKFRPTIFQFTNVVS--NAARLLQACRILNIPQILTE-QYPKGLG 62
Query 65 PILPEI-AETLPEAYEARVKRLGVVNAWADQEFHDAVVATGR-KNLIMAGVTTDVCLIFP 122
P +PE+ AE L + + + ++ + G + +I+ G+ C+
Sbjct 63 PTVPELGAEGLKPHTKTSFSMM-------TESVQSSLKSMGDPQQVILCGIEAQACIACT 115
Query 123 AIDAVAEGFAVQAVIDASGSPSDLSEEFSRQRMHDAGVVLTATNTLMAEIAQDWSSPTGQ 182
A D + G V V DA S S F+ R+ +GV L T ++ ++ QD P +
Sbjct 116 AYDLLERGMEVHIVADAVSSRSQTDRLFALSRLRQSGVFLNTTEGVLLQLVQDAKHPNFK 175
Query 183 QLIGLL 188
++ LL
Sbjct 176 EIQKLL 181
>Q32KX0 Isochorismatase domain-containing protein 2 [Bos taurus]
Length=204
Score = 52.8 bits (125), Expect = 3e-12
Identities = 46/186 (25%), Positives = 85/186 (46%), Gaps = 22/186 (12%)
Query 5 RFTPENSALLLID------HQVGTMQLIKNIDKELAAKQSIALAKMAKILGIPVVITSSQ 58
R P +S L L D H V Q++ +AA+ + K+A++L +P V+T Q
Sbjct 10 RVLPGSSMLFLCDMQEKFRHVVYFRQIVS-----MAAR----MLKVARLLSVPTVLTE-Q 59
Query 59 EDNAQGPILPEIAETLPEAYEARVKRLGVVNAWADQEFHDAVVATGRKNLIMAGVTTDVC 118
GP +PE+ + Y + A Q+ DA +++++ GV T C
Sbjct 60 YPQGLGPTVPELGAQGLQPYSKTCFSM----VPAVQQELDA--RPQLRSVLLCGVETQAC 113
Query 119 LIFPAIDAVAEGFAVQAVIDASGSPSDLSEEFSRQRMHDAGVVLTATNTLMAEIAQDWSS 178
++ A+D + G V V+DA S S + + R+ +G L+ + L+ ++ D +
Sbjct 114 ILQTALDLLDRGLQVHVVVDACTSRSQVDRLVALSRLRQSGAFLSTSEGLIFQLVGDATH 173
Query 179 PTGQQL 184
P +++
Sbjct 174 PQFKEI 179
>Q54RF0 Isochorismatase family protein 2A [Dictyostelium discoideum]
Length=200
Score = 52.4 bits (124), Expect = 4e-12
Identities = 43/197 (22%), Positives = 95/197 (48%), Gaps = 15/197 (8%)
Query 5 RFTPENSALLLIDHQVGTMQLIKNIDKELAAKQSIALAKMAKILGIPVVITSSQEDNAQG 64
R + + +AL + D Q ++ + I++ + +S+ + + IP+++T ++
Sbjct 4 RISSKTTALFICDIQTSFLKTVTQIEELIINNKSLMDSCLE--FKIPIIMTQHDKN---- 57
Query 65 PILPEIAETL--PEAYEARVKRLGVVNAWADQEFHDAVVATGRKNL------IMAGVTTD 116
+ EI E L + + + + V + A + + ++NL I+ G+ +
Sbjct 58 -LFGEIVEPLRYSSSSSSSLSNVMVFDKIAYSMYTKELAQYVKENLPNLKSVILTGLESH 116
Query 117 VCLIFPAIDAVAEGFAVQAVIDASGSPSDLSEEFSRQRMHDAGVVLTATNTLMAEIAQDW 176
VC++ A+D + G+ V + DA+ S + S +RM +GV LT+T T++ +I Q
Sbjct 117 VCVLQTALDLLENGYEVHVIDDATTSVQPKEYKSSLKRMKQSGVFLTSTETVIYQILQSD 176
Query 177 SSPTGQQLIGLLFTDVF 193
+ P +++ L+ +F
Sbjct 177 NHPKFKKIDELIVKRLF 193
>Q96AB3 Isochorismatase domain-containing protein 2 [Homo sapiens]
Length=205
Score = 49.7 bits (117), Expect = 4e-11
Identities = 41/184 (22%), Positives = 79/184 (43%), Gaps = 9/184 (5%)
Query 5 RFTPENSALLLIDHQVGTMQLIKNIDKELAAKQSIALAKMAKILGIPVVITSSQEDNAQG 64
R P +S L L D Q I + ++ + + K+A++L +PV++T Q G
Sbjct 10 RVLPGSSVLFLCDMQEKFRHNIAYFPQIVSV--AARMLKVARLLEVPVMLTE-QYPQGLG 66
Query 65 PILPEIAETLPEAYEARVKRLGVVNAWADQEFHDAVVATGRKNLIMAGVTTDVCLIFPAI 124
P +PE+ E K + QE +++++ G+ C++ +
Sbjct 67 PTVPELGT---EGLRPLAKTCFSMVPALQQELDSRPQL---RSVLLCGIEAQACILNTTL 120
Query 125 DAVAEGFAVQAVIDASGSPSDLSEEFSRQRMHDAGVVLTATNTLMAEIAQDWSSPTGQQL 184
D + G V V+DA S S + + RM +G L+ + L+ ++ D P +++
Sbjct 121 DLLDRGLQVHVVVDACSSRSQVDRLVALARMRQSGAFLSTSEGLILQLVGDAVHPQFKEI 180
Query 185 IGLL 188
L+
Sbjct 181 QKLI 184
>F4JLP5 Dihydrolipoyl dehydrogenase 2, chloroplastic [Arabidopsis
thaliana]
Length=567
Score = 38.9 bits (89), Expect = 4e-07
Identities = 18/44 (41%), Positives = 26/44 (59%), Gaps = 0/44 (0%)
Query 157 DAGVVLTATNTLMAEIAQDWSSPTGQQLIGLLFTDVFPALGAEI 200
D V+T+ + L E DW + G IGL F+DV+ ALG+E+
Sbjct 234 DGKTVITSDHALKLESVPDWIAIVGSGYIGLEFSDVYTALGSEV 277
Score = 20.0 bits (40), Expect = 0.46
Identities = 20/76 (26%), Positives = 33/76 (43%), Gaps = 4/76 (5%)
Query 2 TNQRFTPENSALLLIDHQVGTMQLIKNIDKELAAKQSIALAKMAKILGIPVVITSSQEDN 61
T + F P + + +ID G +L+ ++ A + LA A GI VV + D+
Sbjct 371 TQRGFIPVDERMRVID---GNGKLVPHLYCIGDANGKLMLAHAASAQGISVVEQVTGRDH 427
Query 62 AQGPI-LPEIAETLPE 76
+ +P T PE
Sbjct 428 VLNHLSIPAACFTHPE 443
Score = 18.1 bits (35), Expect = 1.9
Identities = 13/49 (27%), Positives = 25/49 (51%), Gaps = 3/49 (6%)
Query 119 LIFPAIDAVAEGFAVQAV---IDASGSPSDLSEEFSRQRMHDAGVVLTA 164
LI A +A+A G +Q + + A + S++ +E + D+ +TA
Sbjct 512 LIHEASNAIALGTRIQDIKLAVHAHPTLSEVVDELFKAAKVDSPASVTA 560
>A8MS68 Dihydrolipoyl dehydrogenase 1, chloroplastic [Arabidopsis
thaliana]
Length=623
Score = 37.4 bits (85), Expect = 1e-06
Identities = 17/44 (39%), Positives = 26/44 (59%), Gaps = 0/44 (0%)
Query 157 DAGVVLTATNTLMAEIAQDWSSPTGQQLIGLLFTDVFPALGAEI 200
D V+T+ + L E +W + G IGL F+DV+ ALG+E+
Sbjct 237 DGKTVITSDHALKLESVPEWIAIVGSGYIGLEFSDVYTALGSEV 280
Score = 22.3 bits (46), Expect = 0.085
Identities = 22/77 (29%), Positives = 33/77 (43%), Gaps = 4/77 (5%)
Query 1 MTNQRFTPENSALLLIDHQVGTMQLIKNIDKELAAKQSIALAKMAKILGIPVVITSSQED 60
+T + F P + + +ID G L+ N+ A + LA A GI VV S D
Sbjct 373 VTQRGFIPVDERMRVID---GKGTLVPNLYCIGDANGKLMLAHAASAQGISVVEQVSGRD 429
Query 61 NAQGPI-LPEIAETLPE 76
+ + +P T PE
Sbjct 430 HVLNHLSIPAACFTHPE 446
Score = 16.9 bits (32), Expect = 4.4
Identities = 11/30 (37%), Positives = 16/30 (53%), Gaps = 0/30 (0%)
Query 119 LIFPAIDAVAEGFAVQAVIDASGSPSDLSE 148
LI A +A+A G +Q + A + LSE
Sbjct 515 LIHEASNAIALGTRIQDIKLAVHAHPTLSE 544
Lambda K H a alpha
0.320 0.133 0.376 0.792 4.96
Gapped
Lambda K H a alpha sigma
0.267 0.0410 0.140 1.90 42.6 43.6
Effective search space used: 476118
Database: c43a4be951bcfb14269d246c478e6cdb.SwissProt.fasta
Posted date: Jun 2, 2024 6:42 PM
Number of letters in database: 3,459
Number of sequences in database: 13
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 11
Window for multiple hits: 40
Loading
Data ...
