BLASTP 2.12.0+
Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics: Alejandro A. Schaffer,
L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri
I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids
Res. 29:2994-3005.
Database: 6b6ff438bc29b81bb5a3d72ffe8be40d.SwissProt.fasta
6 sequences; 2,521 total letters
Query= ACIAD2737
Length=232
Score E
Sequences producing significant alignments: (Bits) Value
P0AFM1 Intermembrane transport protein PqiA [Shigella flexneri] 83.2 3e-22
P0AFM0 Intermembrane transport protein PqiA [Escherichia coli O6:... 83.2 3e-22
P0AFL9 Intermembrane transport protein PqiA [Escherichia coli (st... 83.2 3e-22
P0AD04 Intermembrane transport protein YebS [Escherichia coli O15... 80.5 3e-21
P0AD03 Intermembrane transport protein YebS [Escherichia coli (st... 80.5 3e-21
P44287 Uncharacterized protein HI_1671 [Haemophilus influenzae (s... 53.1 6e-12
>P0AFM1 Intermembrane transport protein PqiA [Shigella flexneri]
Length=417
Score = 83.2 bits (204), Expect = 3e-22
Identities = 60/204 (29%), Positives = 99/204 (49%), Gaps = 11/204 (5%)
Query 28 EHIPVSELAGCEECDTVYRKVKLAAGERAYCVCCGAEL---YRKTRSLTSLLALVVTALI 84
EH ++ C +CD + +L G++A C CG L + R + AL AL
Sbjct 3 EHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAWDAPRQRPTAYALA--ALF 60
Query 85 IFAIANSFPIVKVELQGNTSQTTLLGAAWTMFQIDRALVGILILFTTFIVPLLDLLLLTY 144
+ ++N FP V + + G TS+ TLL +F D A +G L +VP L+ +
Sbjct 61 MLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTFFLLFVQLVPAFCLITILL 120
Query 145 ILSSVGIFKQRPPYLRLAL-RILFLFRVWGMVEVFLIGVLVTLVKLVGMVVVIPGVALWA 203
+++ + P L+ L R+LF + WGM E+FL GVLV+ VKL+ + G +
Sbjct 121 LVNRA----ELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFVKLMAYGSIGVGSSFLP 176
Query 204 FAILSLL-LVYIASVKIKDIWDEV 226
+ + +L L V + +WD++
Sbjct 177 WCLFCVLQLRAFQCVDRRWLWDDI 200
Score = 69.7 bits (169), Expect = 2e-17
Identities = 41/160 (26%), Positives = 84/160 (53%), Gaps = 2/160 (1%)
Query 58 CVCCGAELY-RKTRSLTSLLALVVTALIIFAIANSFPIVKVELQGNTSQTTLLGAAWTMF 116
C C + Y R+ SL LAL+VT+++++ AN PI+ +L G+ +T+L ++
Sbjct 238 CPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIMVTDLLGSKMPSTILAGVILLW 297
Query 117 QIDRALVGILILFTTFIVPLLDLLLLTYIL-SSVGIFKQRPPYLRLALRILFLFRVWGMV 175
V +I + +VP L ++ + ++ + G K+ + L ++ W M+
Sbjct 298 SEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHGKRDSERMHLIYEVVEFVGRWSMI 357
Query 176 EVFLIGVLVTLVKLVGMVVVIPGVALWAFAILSLLLVYIA 215
+VF+I VL LV++ G++ + P + FA++ ++ ++ A
Sbjct 358 DVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIMTMFSA 397
Score = 16.2 bits (30), Expect = 6.4
Identities = 6/14 (43%), Positives = 10/14 (71%), Gaps = 0/14 (0%)
Query 24 NILGEHIPVSELAG 37
++LG +P + LAG
Sbjct 279 DLLGSKMPSTILAG 292
>P0AFM0 Intermembrane transport protein PqiA [Escherichia coli
O6:H1 (strain CFT073 / ATCC 700928 / UPEC)]
Length=417
Score = 83.2 bits (204), Expect = 3e-22
Identities = 60/204 (29%), Positives = 99/204 (49%), Gaps = 11/204 (5%)
Query 28 EHIPVSELAGCEECDTVYRKVKLAAGERAYCVCCGAEL---YRKTRSLTSLLALVVTALI 84
EH ++ C +CD + +L G++A C CG L + R + AL AL
Sbjct 3 EHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAWDAPRQRPTAYALA--ALF 60
Query 85 IFAIANSFPIVKVELQGNTSQTTLLGAAWTMFQIDRALVGILILFTTFIVPLLDLLLLTY 144
+ ++N FP V + + G TS+ TLL +F D A +G L +VP L+ +
Sbjct 61 MLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTFFLLFVQLVPAFCLITILL 120
Query 145 ILSSVGIFKQRPPYLRLAL-RILFLFRVWGMVEVFLIGVLVTLVKLVGMVVVIPGVALWA 203
+++ + P L+ L R+LF + WGM E+FL GVLV+ VKL+ + G +
Sbjct 121 LVNRA----ELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFVKLMAYGSIGVGSSFLP 176
Query 204 FAILSLL-LVYIASVKIKDIWDEV 226
+ + +L L V + +WD++
Sbjct 177 WCLFCVLQLRAFQCVDRRWLWDDI 200
Score = 69.7 bits (169), Expect = 2e-17
Identities = 41/160 (26%), Positives = 84/160 (53%), Gaps = 2/160 (1%)
Query 58 CVCCGAELY-RKTRSLTSLLALVVTALIIFAIANSFPIVKVELQGNTSQTTLLGAAWTMF 116
C C + Y R+ SL LAL+VT+++++ AN PI+ +L G+ +T+L ++
Sbjct 238 CPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIMVTDLLGSKMPSTILAGVILLW 297
Query 117 QIDRALVGILILFTTFIVPLLDLLLLTYIL-SSVGIFKQRPPYLRLALRILFLFRVWGMV 175
V +I + +VP L ++ + ++ + G K+ + L ++ W M+
Sbjct 298 SEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHGKRDSERMHLIYEVVEFVGRWSMI 357
Query 176 EVFLIGVLVTLVKLVGMVVVIPGVALWAFAILSLLLVYIA 215
+VF+I VL LV++ G++ + P + FA++ ++ ++ A
Sbjct 358 DVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIMTMFSA 397
Score = 16.2 bits (30), Expect = 6.4
Identities = 6/14 (43%), Positives = 10/14 (71%), Gaps = 0/14 (0%)
Query 24 NILGEHIPVSELAG 37
++LG +P + LAG
Sbjct 279 DLLGSKMPSTILAG 292
>P0AFL9 Intermembrane transport protein PqiA [Escherichia coli
(strain K12)]
Length=417
Score = 83.2 bits (204), Expect = 3e-22
Identities = 60/204 (29%), Positives = 99/204 (49%), Gaps = 11/204 (5%)
Query 28 EHIPVSELAGCEECDTVYRKVKLAAGERAYCVCCGAEL---YRKTRSLTSLLALVVTALI 84
EH ++ C +CD + +L G++A C CG L + R + AL AL
Sbjct 3 EHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAWDAPRQRPTAYALA--ALF 60
Query 85 IFAIANSFPIVKVELQGNTSQTTLLGAAWTMFQIDRALVGILILFTTFIVPLLDLLLLTY 144
+ ++N FP V + + G TS+ TLL +F D A +G L +VP L+ +
Sbjct 61 MLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTFFLLFVQLVPAFCLITILL 120
Query 145 ILSSVGIFKQRPPYLRLAL-RILFLFRVWGMVEVFLIGVLVTLVKLVGMVVVIPGVALWA 203
+++ + P L+ L R+LF + WGM E+FL GVLV+ VKL+ + G +
Sbjct 121 LVNRA----ELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFVKLMAYGSIGVGSSFLP 176
Query 204 FAILSLL-LVYIASVKIKDIWDEV 226
+ + +L L V + +WD++
Sbjct 177 WCLFCVLQLRAFQCVDRRWLWDDI 200
Score = 69.7 bits (169), Expect = 2e-17
Identities = 41/160 (26%), Positives = 84/160 (53%), Gaps = 2/160 (1%)
Query 58 CVCCGAELY-RKTRSLTSLLALVVTALIIFAIANSFPIVKVELQGNTSQTTLLGAAWTMF 116
C C + Y R+ SL LAL+VT+++++ AN PI+ +L G+ +T+L ++
Sbjct 238 CPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIMVTDLLGSKMPSTILAGVILLW 297
Query 117 QIDRALVGILILFTTFIVPLLDLLLLTYIL-SSVGIFKQRPPYLRLALRILFLFRVWGMV 175
V +I + +VP L ++ + ++ + G K+ + L ++ W M+
Sbjct 298 SEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHGKRDSERMHLIYEVVEFVGRWSMI 357
Query 176 EVFLIGVLVTLVKLVGMVVVIPGVALWAFAILSLLLVYIA 215
+VF+I VL LV++ G++ + P + FA++ ++ ++ A
Sbjct 358 DVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIMTMFSA 397
Score = 16.2 bits (30), Expect = 6.4
Identities = 6/14 (43%), Positives = 10/14 (71%), Gaps = 0/14 (0%)
Query 24 NILGEHIPVSELAG 37
++LG +P + LAG
Sbjct 279 DLLGSKMPSTILAG 292
>P0AD04 Intermembrane transport protein YebS [Escherichia coli
O157:H7]
Length=427
Score = 80.5 bits (197), Expect = 3e-21
Identities = 47/205 (23%), Positives = 103/205 (50%), Gaps = 6/205 (3%)
Query 22 SLNILGEHIPVSELAGCEECDTVYRKVKLAAGERAYCVCCGAELYR-KTRSLTSLLALVV 80
++ +GE +P + C +CD ++ ++ + + AYC C A++ + SLT L A+
Sbjct 15 TVRAIGEELPRGDYQRCPQCDMLFSLPEINSHQSAYCPRCQAKIRDGRDWSLTRLAAMAF 74
Query 81 TALIIFAIANSFPIVKVELQGNTSQTTLLGAAWTMFQIDRALVGILILFTTFIVPLLDLL 140
T L++ A P++ + L G ++ W M + A+ G ++ F PL+ +
Sbjct 75 TMLLLMPFAWGEPLLHIWLLGIRIDANVMQGIWQMTKQGDAITGSMVFFCVIGAPLILVT 134
Query 141 LLTYILSSVGIFKQRPPYLRLALRILFLFRVWGMVEVFLIGVLVTLVKLVGMVVVIPGVA 200
+ Y+ + P L + R+ + W M++++L+G+ V +K+ + GV
Sbjct 135 SIAYLWFGNRLGMNLRPVLLMLERL----KEWVMLDIYLVGIGVASIKVQDYAHIQAGVG 190
Query 201 LWAF-AILSLLLVYIASVKIKDIWD 224
L++F A++ L V ++ + ++++W+
Sbjct 191 LFSFVALVILTTVTLSHLNVEELWE 215
Score = 52.8 bits (125), Expect = 9e-12
Identities = 40/174 (23%), Positives = 85/174 (49%), Gaps = 4/174 (2%)
Query 54 ERAYCVCCGAEL-YRKTRSLTSLLALVVTALIIFAIANSFPIVKVELQGNTSQTTLLGAA 112
+R C C L R+ SL A ++ ++++ AN PI + L G + T+L
Sbjct 244 QRGRCPRCHIPLRLRRRHSLQKCWAALLASIVLLLPANLLPISIIYLNGGRQEDTILSGI 303
Query 113 WTMFQIDRALVGILILFTTFIVPLLDLLLLTYILSSVGIFKQRPPYLR-LALRILFLFRV 171
++ + A+ GI + + +VP ++++ +L S+ Q+ R L LR++
Sbjct 304 MSLASSNIAVAGI-VFIASILVPFTKVIVMFTLLLSIHFKCQQGLRTRILLLRMVTWIGR 362
Query 172 WGMVEVFLIGVLVTLVKLVGMVVVIPGVALWAF-AILSLLLVYIASVKIKDIWD 224
W M+++F+I + ++L+ ++ G A + F A + L ++ + + + +WD
Sbjct 363 WSMLDLFVISLTMSLINRDQILAFTMGPAAFYFGAAVILTILAVEWLDSRLLWD 416
>P0AD03 Intermembrane transport protein YebS [Escherichia coli
(strain K12)]
Length=427
Score = 80.5 bits (197), Expect = 3e-21
Identities = 47/205 (23%), Positives = 103/205 (50%), Gaps = 6/205 (3%)
Query 22 SLNILGEHIPVSELAGCEECDTVYRKVKLAAGERAYCVCCGAELYR-KTRSLTSLLALVV 80
++ +GE +P + C +CD ++ ++ + + AYC C A++ + SLT L A+
Sbjct 15 TVRAIGEELPRGDYQRCPQCDMLFSLPEINSHQSAYCPRCQAKIRDGRDWSLTRLAAMAF 74
Query 81 TALIIFAIANSFPIVKVELQGNTSQTTLLGAAWTMFQIDRALVGILILFTTFIVPLLDLL 140
T L++ A P++ + L G ++ W M + A+ G ++ F PL+ +
Sbjct 75 TMLLLMPFAWGEPLLHIWLLGIRIDANVMQGIWQMTKQGDAITGSMVFFCVIGAPLILVT 134
Query 141 LLTYILSSVGIFKQRPPYLRLALRILFLFRVWGMVEVFLIGVLVTLVKLVGMVVVIPGVA 200
+ Y+ + P L + R+ + W M++++L+G+ V +K+ + GV
Sbjct 135 SIAYLWFGNRLGMNLRPVLLMLERL----KEWVMLDIYLVGIGVASIKVQDYAHIQAGVG 190
Query 201 LWAF-AILSLLLVYIASVKIKDIWD 224
L++F A++ L V ++ + ++++W+
Sbjct 191 LFSFVALVILTTVTLSHLNVEELWE 215
Score = 52.8 bits (125), Expect = 9e-12
Identities = 40/174 (23%), Positives = 85/174 (49%), Gaps = 4/174 (2%)
Query 54 ERAYCVCCGAEL-YRKTRSLTSLLALVVTALIIFAIANSFPIVKVELQGNTSQTTLLGAA 112
+R C C L R+ SL A ++ ++++ AN PI + L G + T+L
Sbjct 244 QRGRCPRCHIPLRLRRRHSLQKCWAALLASIVLLLPANLLPISIIYLNGGRQEDTILSGI 303
Query 113 WTMFQIDRALVGILILFTTFIVPLLDLLLLTYILSSVGIFKQRPPYLR-LALRILFLFRV 171
++ + A+ GI + + +VP ++++ +L S+ Q+ R L LR++
Sbjct 304 MSLASSNIAVAGI-VFIASILVPFTKVIVMFTLLLSIHFKCQQGLRTRILLLRMVTWIGR 362
Query 172 WGMVEVFLIGVLVTLVKLVGMVVVIPGVALWAF-AILSLLLVYIASVKIKDIWD 224
W M+++F+I + ++L+ ++ G A + F A + L ++ + + + +WD
Sbjct 363 WSMLDLFVISLTMSLINRDQILAFTMGPAAFYFGAAVILTILAVEWLDSRLLWD 416
>P44287 Uncharacterized protein HI_1671 [Haemophilus influenzae
(strain ATCC 51907 / DSM 11121 / KW20 / Rd)]
Length=416
Score = 53.1 bits (126), Expect = 6e-12
Identities = 46/198 (23%), Positives = 83/198 (42%), Gaps = 7/198 (4%)
Query 31 PVSELAGCEECDTVYRKVK-LAAGERAYCVCCGAELYRKTR-SLTSLLALVVTALIIFAI 88
P ++ C ECD L + E+A C C L TR SL + ++ LI+
Sbjct 9 PPFKILKCTECDACINVPSILQSNEQAECPRCHHLLASGTRWSLHRCAMIALSILILMPF 68
Query 89 ANSFPIVKVELQGNTSQTTLLGAAWTMFQIDRALVGILILFTTFIVPLLDLLLLTYILSS 148
+ ++P++ + L G ++ W M +I ++P + LL +L
Sbjct 69 SLNYPLLSLHLLGIKIDASIWDGIWKMAVGGYEYTAFMIFICAVVMP-ITFALLVIMLWL 127
Query 149 VGIFKQRPPYLRLALRILFLFRVWGMVEVFLIGVLVTLVKLVGMVVVIPGVALWAFAILS 208
IF+ +P R L L + W M +V+L+ + VT+ K+ + + L F +
Sbjct 128 AKIFQIKP---RSVLLFLGYIKAWVMFDVYLVALGVTIFKVREYATLEINIYLIPFIFTA 184
Query 209 LLLVYI-ASVKIKDIWDE 225
LL + + + +W E
Sbjct 185 LLTTLLFIKLNLSALWQE 202
Score = 51.6 bits (122), Expect = 2e-11
Identities = 41/181 (23%), Positives = 83/181 (46%), Gaps = 9/181 (5%)
Query 54 ERAYCVCCGAELYRKTR-SLTSLLALVVTALIIFAIANSFPIVKVELQGNTSQTTLLGAA 112
++ C C + L + L + A ++ +I+ AN PI + L G S+ TL+
Sbjct 237 QKICCPRCQSPLNTSDKLKLQATWATLIAGIIMLFPANLLPISGIYLTGALSEDTLISGV 296
Query 113 WTMFQIDRALVGILILFTTFIVPLLDLLLLTYILSSVGIFKQRPPYLRLALRILFLFRV- 171
+ + V ++ F + VP+ + ++ Y+L+ V Q ++ +R+L L
Sbjct 297 ISFVKSGSYFVAFVVFFASIFVPISKIFIMLYLLACVHFKWQHS--IKWQMRLLHLVHFV 354
Query 172 --WGMVEVFLIGVLVTLVKLVGMV--VVIPGVALWAFAILSLLLVYIASVKIKDIWDEVD 227
W M+++F++ ++++LV ++ V PG + A+ +L + K IW D
Sbjct 355 GRWSMLDLFVLALMMSLVTRGEIINFTVGPGAFYFGAAVFCTML-STSQFDSKLIWKIYD 413
Query 228 R 228
R
Sbjct 414 R 414
Lambda K H a alpha
0.331 0.143 0.432 0.792 4.96
Gapped
Lambda K H a alpha sigma
0.267 0.0410 0.140 1.90 42.6 43.6
Effective search space used: 441391
Database: 6b6ff438bc29b81bb5a3d72ffe8be40d.SwissProt.fasta
Posted date: May 12, 2024 9:00 PM
Number of letters in database: 2,521
Number of sequences in database: 6
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 11
Window for multiple hits: 40
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Data ...
