BLASTP 2.12.0+
Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics: Alejandro A. Schaffer,
L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri
I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids
Res. 29:2994-3005.
Database: ca96eaa63c35b328804186b38321af07.SwissProt.fasta
19 sequences; 8,680 total letters
Query= ACIAD2888
Length=542
Score E
Sequences producing significant alignments: (Bits) Value
Q9PC24 Glutamine-dependent NAD(+) synthetase [Xylella fastidiosa ... 464 3e-162
Q87D47 Glutamine-dependent NAD(+) synthetase [Xylella fastidiosa ... 461 4e-161
Q9X0Y0 Glutamine-dependent NAD(+) synthetase [Thermotoga maritima... 461 2e-160
P74292 Glutamine-dependent NAD(+) synthetase [Synechocystis sp. (... 449 3e-156
O67091 Glutamine-dependent NAD(+) synthetase [Aquifex aeolicus (s... 424 3e-146
Q03638 Glutamine-dependent NAD(+) synthetase [Rhodobacter capsula... 416 2e-143
Q8KEX2 NH(3)-dependent NAD(+) synthetase [Chlorobaculum tepidum (... 157 4e-47
A5G7Y9 NH(3)-dependent NAD(+) synthetase [Geotalea uraniireducens... 152 3e-45
B3ELE3 NH(3)-dependent NAD(+) synthetase [Chlorobium phaeobactero... 152 3e-45
B3E4K8 NH(3)-dependent NAD(+) synthetase [Trichlorobacter lovleyi... 150 9e-45
P9WJJ3 Glutamine-dependent NAD(+) synthetase [Mycobacterium tuber... 129 9e-35
O25096 NH(3)-dependent NAD(+) synthetase [Helicobacter pylori (st... 104 3e-28
Q75SP7 (R)-stereoselective amidase [Pseudomonas sp] 84.0 4e-21
Q9UYV8 Nitrilase [Pyrococcus abyssi (strain GE5 / Orsay)] 67.8 1e-15
Q81RP3 NH(3)-dependent NAD(+) synthetase [Bacillus anthracis] 66.6 3e-15
P08164 NH(3)-dependent NAD(+) synthetase [Bacillus subtilis (stra... 66.2 4e-15
Q6IA69 Glutamine-dependent NAD(+) synthetase [Homo sapiens] 57.0 1e-11
Q9VYA0 Glutamine-dependent NAD(+) synthetase [Drosophila melanoga... 55.8 3e-11
Q711T7 Glutamine-dependent NAD(+) synthetase [Mus musculus] 55.1 5e-11
>Q9PC24 Glutamine-dependent NAD(+) synthetase [Xylella fastidiosa
(strain 9a5c)]
Length=545
Score = 464 bits (1194), Expect = 3e-162
Identities = 252/540 (47%), Positives = 347/540 (64%), Gaps = 7/540 (1%)
Query 5 KIALAQFSPHIGNIDSNAQRMVEQANEAKKQN-ADLIIFPELSVIGYPAEDLLLRPNLNK 63
+IA+AQF +G + NA+R++ +A+ ++ AD+++FPEL++ GYP EDLLLRP
Sbjct 6 RIAMAQFDFPVGAVAQNAERIIALIEQARDEHGADVVMFPELALSGYPPEDLLLRPGFLA 65
Query 64 RMQKAFQQLKEVKD-IVMVFGFVHQTEEGHRYNSAAVMKDGVVLGVYNKHNLPNYSVFDE 122
Q A +++ IV V G+ Q+ YN A+V+ DG V Y K LPNY+VFDE
Sbjct 66 HCQVAIERIAAATHGIVAVVGWP-QSAGSVVYNVASVLCDGQVEQTYRKRELPNYAVFDE 124
Query 123 KRYFS--PGHQHLVFEYLGHKFGVLICEDIWSINTVKQLSKLNVETVLVLNASPYEVGKP 180
+RYF P VF+ G GVLICED+W + E VLV NASPYE GK
Sbjct 125 RRYFEVDPNGSRCVFKVKGVPVGVLICEDLWFSEPLADTVCGGAELVLVPNASPYERGKH 184
Query 181 QHRVQTLTELSKQLNVHLVYLNQVGGQDDLIFDGSSFIINHDGEVAFQAPSFKEELYYSE 240
R L E +++ + YLN VGGQD L+FDG+S +++ G V A +F ++ +
Sbjct 185 AQRDALLAERARETGAAIAYLNVVGGQDALVFDGASVVVDGHGRVHPAAAAFSDQWLVVD 244
Query 241 FDIEQKRYKKID--PAPALDTIAEIYQSLVMATRDYVQRSGFSGVILGLSGGIDSALTLA 298
+ ++R+ + + A +++++V +DY +++GFS V +GLSGGIDSAL LA
Sbjct 245 YMRSERRFVPLQWVAESEVSINALVWRAVVRGVQDYCRKNGFSKVWVGLSGGIDSALVLA 304
Query 299 IAADAIGADKVQAVMMPYTYTSQISVEDATEQARRMGVTFGIAEIHPIVNSFMQTLYPFF 358
IA DA+GAD+V AV +P YT+++S + A EQ +GV I P+ + L P F
Sbjct 305 IAVDALGADQVTAVRLPSRYTAELSNDLAAEQCHSLGVRLETVAIEPVFEGLLAALGPLF 364
Query 359 GNAPADATEENLQARARGTLLMGLSNKFGNLVLSTGNKSELAVGYCTLYGDMVGGFAVLK 418
DATEENLQ+R+RG +LM L+NKFG L+L+TGNKSE AVGY T+YGDM GG+A LK
Sbjct 365 AGMAPDATEENLQSRSRGVILMALANKFGGLLLTTGNKSEYAVGYATIYGDMCGGYAPLK 424
Query 419 DVYKTIVFELAKYRNTLSETPVIPERVITRPPSAELRPDQKDQDSLPAYDILDAILYAYI 478
D+YK+ VFELA++RNT+S+ IP VI RPPSAELR Q DQDSLP Y++LD IL Y+
Sbjct 425 DIYKSQVFELAQWRNTVSDVLAIPPGVIHRPPSAELRAQQTDQDSLPPYEVLDGILSLYV 484
Query 479 EEDQSQSDIIAKGFDKEVVEKVIRLVDRNEYKRRQGAIGPRISSRAFSRERRYPIVNGWR 538
+++QS+ DIIA G+ VV+ V+ LV NE+KR Q A GP++S RAF RERRYPI N +R
Sbjct 485 DQEQSREDIIAAGYAAGVVDYVLNLVKINEWKRHQAAPGPKVSQRAFGRERRYPISNAYR 544
>Q87D47 Glutamine-dependent NAD(+) synthetase [Xylella fastidiosa
(strain Temecula1 / ATCC 700964)]
Length=545
Score = 461 bits (1186), Expect = 4e-161
Identities = 251/540 (46%), Positives = 346/540 (64%), Gaps = 7/540 (1%)
Query 5 KIALAQFSPHIGNIDSNAQRMVEQANEAKKQN-ADLIIFPELSVIGYPAEDLLLRPNLNK 63
+IA+AQF +G + NA+R++ +A+ ++ AD+++FPEL++ GYP EDLLLRP
Sbjct 6 RIAMAQFDFPVGAVAQNAERIIALIEQARDEHGADVVMFPELALSGYPPEDLLLRPGFLA 65
Query 64 RMQKAFQQLKEVKD-IVMVFGFVHQTEEGHRYNSAAVMKDGVVLGVYNKHNLPNYSVFDE 122
Q A +++ IV V G+ Q+ YN A+V+ DG V Y K LPNY+VFDE
Sbjct 66 HCQVAIERIAAATHGIVAVVGWP-QSAGSVVYNVASVLCDGQVEQTYRKRELPNYAVFDE 124
Query 123 KRYFS--PGHQHLVFEYLGHKFGVLICEDIWSINTVKQLSKLNVETVLVLNASPYEVGKP 180
+RYF P VF+ G GVLICED+W + E VLV NASPYE GK
Sbjct 125 RRYFEVDPNGSRCVFKVKGVPVGVLICEDLWFSEPLADTVCGGAELVLVPNASPYERGKH 184
Query 181 QHRVQTLTELSKQLNVHLVYLNQVGGQDDLIFDGSSFIINHDGEVAFQAPSFKEELYYSE 240
R L E +++ + YLN VGGQD L+FDG+S +++ G V A +F ++ +
Sbjct 185 AQRDALLAERARETGAAIAYLNVVGGQDALVFDGASVVVDGHGRVHPAAAAFSDQWLVVD 244
Query 241 FDIEQKRYKKID--PAPALDTIAEIYQSLVMATRDYVQRSGFSGVILGLSGGIDSALTLA 298
+ ++R+ + + A +++++V +DY +++GFS V +GLSGGIDSAL LA
Sbjct 245 YMRSERRFVPLQWVVESEVSINALVWRAVVRGVQDYCRKNGFSKVWVGLSGGIDSALVLA 304
Query 299 IAADAIGADKVQAVMMPYTYTSQISVEDATEQARRMGVTFGIAEIHPIVNSFMQTLYPFF 358
IA DA+GAD+V AV +P YT+++S + A EQ +GV I P+ + L P F
Sbjct 305 IAVDALGADQVTAVRLPSRYTAELSNDLAAEQCHSLGVRLETVAIEPVFEGLLAALGPLF 364
Query 359 GNAPADATEENLQARARGTLLMGLSNKFGNLVLSTGNKSELAVGYCTLYGDMVGGFAVLK 418
DATEENLQ+R+RG +LM L+NKFG L+L+TGNKSE AVGY T+YGDM GG+A LK
Sbjct 365 AGMAPDATEENLQSRSRGVILMALANKFGGLLLTTGNKSEYAVGYATIYGDMCGGYAPLK 424
Query 419 DVYKTIVFELAKYRNTLSETPVIPERVITRPPSAELRPDQKDQDSLPAYDILDAILYAYI 478
D+YK+ VFELA++RN +S+ IP VI RPPSAELR Q DQDSLP Y++LD IL Y+
Sbjct 425 DIYKSQVFELAQWRNRVSDVLAIPPGVIHRPPSAELRAQQTDQDSLPPYEVLDGILSLYV 484
Query 479 EEDQSQSDIIAKGFDKEVVEKVIRLVDRNEYKRRQGAIGPRISSRAFSRERRYPIVNGWR 538
+++QS+ DIIA G+ VV+ V+ LV NE+KR Q A GP++S RAF RERRYPI N +R
Sbjct 485 DQEQSREDIIAAGYAAGVVDYVLNLVRINEWKRHQAAPGPKVSQRAFGRERRYPISNAYR 544
>Q9X0Y0 Glutamine-dependent NAD(+) synthetase [Thermotoga maritima
(strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826
/ MSB8)]
Length=576
Score = 461 bits (1185), Expect = 2e-160
Identities = 246/579 (42%), Positives = 358/579 (62%), Gaps = 47/579 (8%)
Query 1 MKSFKIALAQFSPHIGNIDSNAQRMVEQANEAKKQNADLIIFPELSVIGYPAEDLLLRPN 60
MK ++ LAQ +P +G+ + N ++ +E A+ + +DL++FPEL + GYP EDL+LR +
Sbjct 1 MKRLRVTLAQLNPTLGDFEGNLKKAIEALRVAEDRGSDLLVFPELFLPGYPPEDLMLRLS 60
Query 61 L---NKRMQKAFQQLKEVKDIVMVFGFVHQTEEGHRYNSAAVMKDGVVLGVYNKHNLPNY 117
N++ + F Q + ++ GF+ E+ YN+AAV+KDG +LGVY K +LPNY
Sbjct 61 FLRENRKYLQKFAQHTRNLGVTVLMGFIDSDEDA--YNAAAVVKDGEILGVYRKISLPNY 118
Query 118 SVFDEKRYFSPGHQHLVFEYLGHKFGVLICEDIWSINTVKQLSKLN----VETVLVLNAS 173
VFDE+RYF PG + LV + K GV ICEDIW N V+ + L+ V + L+AS
Sbjct 119 GVFDERRYFKPGEELLVVKIGNIKVGVTICEDIW--NPVEPSASLSLGEGVHLIANLSAS 176
Query 174 PYEVGKPQHRVQTLTELSKQLNVHLVYLNQVGGQDDLIFDGSSFIINHDGEVAFQAPSFK 233
PY VGKP R L+ + +V + Y N VGGQD+L+FDG S +++ GEV F+
Sbjct 177 PYHVGKPVLRKDYLSMKAYDYHVAMAYCNMVGGQDELVFDGGSMVVDASGEVINYGKLFE 236
Query 234 EELYYSEFDIEQ----------KRYKK------------------------IDPAPALDT 259
EE+ + D+++ +RY K ++P P +
Sbjct 237 EEIITVDLDLDENLRVSLVDPRRRYMKTQNYPVKTVEAGNLREKSGHFEPVVNPLPVREE 296
Query 260 IAEIYQSLVMATRDYVQRSGFSGVILGLSGGIDSALTLAIAADAIGADKVQAVMMPYTYT 319
E++++L+ RDYV+++GF V++GLSGG+DS+L IA +A+G + V+ V+MP YT
Sbjct 297 --EMFRALITGLRDYVRKNGFEKVVIGLSGGMDSSLVAVIATEALGKENVKGVLMPSMYT 354
Query 320 SQISVEDATEQARRMGVTFGIAEIHPIVNSFMQTLYPFFGNAPADATEENLQARARGTLL 379
S+ S+EDA A+ +G+ I I + +S+++TL F D TEENLQAR RG L
Sbjct 355 SKESIEDAQTLAKNLGIETFIIPITDVFHSYLETLKGVFAGREPDITEENLQARIRGNYL 414
Query 380 MGLSNKFGNLVLSTGNKSELAVGYCTLYGDMVGGFAVLKDVYKTIVFELAKYRNTLSETP 439
M LSNKFG LVL+TGNKSE+A GY TLYGDM GGFAV+KDVYKT V+ + ++ N+
Sbjct 415 MALSNKFGWLVLTTGNKSEMATGYATLYGDMAGGFAVIKDVYKTDVYRIGRWYNSWRGKE 474
Query 440 VIPERVITRPPSAELRPDQKDQDSLPAYDILDAILYAYIEEDQSQSDIIAKGFDKEVVEK 499
+IPE + +PP+AELRP Q DQ+ LP Y++LD IL YIEE +I +KGFD++ V
Sbjct 475 IIPENIFVKPPTAELRPGQTDQEKLPPYEVLDEILRLYIEEGLDPEEIASKGFDRKTVLD 534
Query 500 VIRLVDRNEYKRRQGAIGPRISSRAFSRERRYPIVNGWR 538
V ++ +NEYKR+Q AIG +IS+RAF ++ R PI N ++
Sbjct 535 VTEMIRKNEYKRKQAAIGVKISTRAFGKDWRMPITNRFK 573
>P74292 Glutamine-dependent NAD(+) synthetase [Synechocystis sp.
(strain PCC 6803 / Kazusa)]
Length=558
Score = 449 bits (1155), Expect = 3e-156
Identities = 249/557 (45%), Positives = 353/557 (63%), Gaps = 24/557 (4%)
Query 4 FKIALAQFSPHIGNIDSNAQRMVEQANEAKKQNADLIIFPELSVIGYPAEDLLLRPNLNK 63
F IALAQ +P IG I NA+++V A +A+ + ADL++ PEL++ GYP +DLLL P+ +
Sbjct 2 FTIALAQLNPTIGAIAENAEKIVTAALQAQARGADLLLTPELALCGYPPKDLLLNPSFVE 61
Query 64 RMQKAFQQLKEVK--DIVMVFGFV---HQTE-EGHR--YNSAAVMKDGVVLGVYNKHNLP 115
++++ Q L E I ++ G V HQ E +G + +NSA +++ G + ++K LP
Sbjct 62 QLEEELQWLAEKMPPSIAILVGTVTPHHQAERQGQKKLWNSAVLIEQGQIKQWFHKCLLP 121
Query 116 NYSVFDEKRYFSPGHQHLVFEYLGHKFGVLICEDIWS-----------INTVKQLSKLNV 164
Y VFDE RYF+ + F Y K GV ICED+W+ +N + L V
Sbjct 122 TYDVFDEDRYFASAAKSEYFIYKNVKIGVTICEDLWNDEAFWGQKFYQVNPLMDLIDQGV 181
Query 165 ETVLVLNASPYEVGKPQHRVQTLTELSKQLNVHLVYLNQVGGQDDLIFDGSSFIINHDGE 224
V+ L+ASPY GK R ++ +K+ NV L+Y+NQVGG DDLIFDG SF +N G+
Sbjct 182 NLVVNLSASPYSCGKHYLRESLISHSAKRFNVPLIYVNQVGGNDDLIFDGGSFAVNSQGK 241
Query 225 VAFQAPSFKEELYYSEFDIEQKRY--KKIDPAPALDTIAEIYQSLVMATRDYVQRSGFSG 282
+ ++P F+E+L +D+ +K+ P +DT E++Q+LV+ DY+ + GFS
Sbjct 242 IIGRSPLFQEDLALLSYDLSSGELTGQKLASLPMVDT-EELWQALVLGVGDYLHKCGFSK 300
Query 283 VILGLSGGIDSALTLAIAADAIGADKVQAVMMPYTYTSQISVEDATEQARRMGVTFGIAE 342
ILGLSGGIDS+L AIA +A+G + V ++MP Y+S S++DA A+ +G+
Sbjct 301 AILGLSGGIDSSLVAAIAVEALGKENVLGILMPSPYSSDHSIQDALALAKNLGMNTQTIP 360
Query 343 IHPIVNSFMQTLYPFFGNAPADATEENLQARARGTLLMGLSNKFGNLVLSTGNKSELAVG 402
I PI+ ++ Q L P F +AP EENLQ+R RG LLM ++NKFG+L+LSTGNKSELAVG
Sbjct 361 IGPIMATYDQALVPLFQDAPFGLAEENLQSRIRGNLLMAIANKFGHLLLSTGNKSELAVG 420
Query 403 YCTLYGDMVGGFAVLKDVYKTIVFELAKYRNTLSETPVIPERVITRPPSAELRPDQKDQD 462
YCTLYGDM GG A + DV KT VFEL ++ N E +IP V+T+PPSAEL+P Q D D
Sbjct 421 YCTLYGDMNGGLAAIADVPKTQVFELCRWLN--REQTIIPPSVLTKPPSAELKPGQVDTD 478
Query 463 SLPAYDILDAILYAYIEEDQSQSDIIAKGFDKEVVEKVIRLVDRNEYKRRQGAIGPRISS 522
SLP YD+LD IL +E+ QS +II GF++EVV K+ +LV ++E+KRRQ A G +++
Sbjct 479 SLPPYDVLDGILGRLVEKHQSPQEIINAGFEREVVLKICQLVQKSEFKRRQAAPGLKVTD 538
Query 523 RAFSRERRYPIVNGWRP 539
RAF R PI + P
Sbjct 539 RAFGSGWRMPIAQAFHP 555
>O67091 Glutamine-dependent NAD(+) synthetase [Aquifex aeolicus
(strain VF5)]
Length=567
Score = 424 bits (1090), Expect = 3e-146
Identities = 236/567 (42%), Positives = 343/567 (60%), Gaps = 44/567 (8%)
Query 6 IALAQFSPHIGNIDSNAQRMVEQANEAKKQNADLIIFPELSVIGYPAEDLLLRPNLNKRM 65
+ LAQ + +G+++ N +++++ +E +++ +I FPELS+ GYP EDLLL+P+ K
Sbjct 4 LTLAQLNFTVGDVEGNKEKILKVIDEYSEKS-HIIAFPELSLSGYPPEDLLLQPHFLKEC 62
Query 66 QKAFQQL---KEVKDIVMVFGFVHQTEEGHRYNSAAVMKDGVVLGVYNKHNLPNYSVFDE 122
+KAF Q+ D+++ G + E YN+ AV+ G VLG+Y KH LPNYSVFDE
Sbjct 63 EKAFDQIIHHTRNYDVIVAVGLPYY--EFDLYNALAVIHRGEVLGIYKKHFLPNYSVFDE 120
Query 123 KRYFSPGHQHLVFEYLGHKFGVLICEDIWSINTVKQLSKLN-VETVLVLNASPYEVGKPQ 181
RYF G + L+ E GHK ICEDIW + V++ + L+ E ++ +NASPY V K
Sbjct 121 YRYFRKGEEPLMIEVNGHKVSFSICEDIWYPDGVERQTALSGAELIVNVNASPYHVNKYS 180
Query 182 HRVQTLTELSKQLNVHLVYLNQVGGQDDLIFDGSSFIINHDGEVAFQAPSFKEELYYSEF 241
+ L ++ + Y+N VGGQD+L+FDG S +I+ G++ +A +F+E++
Sbjct 181 FKESFLKSRAEDNLCFVAYVNLVGGQDELVFDGRSIVISPFGKLVARAKAFEEDILTVTL 240
Query 242 DIEQKRYKK---------------------------------IDPAPALDTIAEIYQSLV 268
D+ + + K+ I+ P + EIY +L
Sbjct 241 DLGEAKRKRLLDLRWREGSYGREKVNVKRSVSLPDKEFFRGRIEENPKEEE--EIYAALK 298
Query 269 MATRDYVQRSGFSGVILGLSGGIDSALTLAIAADAIGADKVQAVMMPYTYTSQISVEDAT 328
++ RDYV+++GF V+LGLSGGIDS+ +A DA+G + V+ V MP ++S+ S EDA
Sbjct 299 LSLRDYVRKNGFEKVVLGLSGGIDSSFVACLAVDALGRENVKGVYMPSQFSSKESYEDAK 358
Query 329 EQARRMGVTFGIAEIHPIVNSFMQTLYPFFGNAPADATEENLQARARGTLLMGLSNKFGN 388
A+ +G+ F + I I ++ D +EN+QAR R +L SNKF
Sbjct 359 ALAQNLGIEFHVIPIKEIYRAYFNEFEKEICEITFDVADENIQARIRANILFYFSNKFRY 418
Query 389 LVLSTGNKSELAVGYCTLYGDMVGGFAVLKDVYKTIVFELAKYRNTLSETPVIPERVITR 448
LVLST NKSE AVGY T+YGDM GGFA +KDVYKT V++LA+YRN++S P IPERV +
Sbjct 419 LVLSTSNKSETAVGYTTIYGDMAGGFAPIKDVYKTWVYKLARYRNSIS--PDIPERVFKK 476
Query 449 PPSAELRPDQKDQDSLPAYDILDAILYAYIEEDQSQSDIIAKGFDKEVVEKVIRLVDRNE 508
PPSAELRP+Q DQD LP Y+ILD IL YIEE+ S +II KG ++ V K I ++ +NE
Sbjct 477 PPSAELRPNQTDQDVLPPYEILDQILMLYIEENLSPEEIIRKGLPRDAVYKTINMIRKNE 536
Query 509 YKRRQGAIGPRISSRAFSRERRYPIVN 535
YKR+Q IGP+I+SRAF ++ R P+ N
Sbjct 537 YKRKQAPIGPKITSRAFGKDWRMPVTN 563
>Q03638 Glutamine-dependent NAD(+) synthetase [Rhodobacter capsulatus]
Length=552
Score = 416 bits (1069), Expect = 2e-143
Identities = 233/547 (43%), Positives = 328/547 (60%), Gaps = 14/547 (3%)
Query 4 FKIALAQFSPHIGNIDSNAQRMVEQANEAKKQNADLIIFPELSVIGYPAEDLLLRPNLNK 63
F+I LAQ +P +G + +NA++ + + ADL+ PE+ + GY +DL+L+P +
Sbjct 5 FRITLAQLNPTVGALAANAEKAMAAWQAGRAAGADLVALPEMFLTGYQTQDLVLKPAFLR 64
Query 64 RMQKAFQQL--KEVKDIVMVFGFVHQTEEGHRYNSAAVMKDGVVLGVYNKHNLPNYSVFD 121
A L + V + G + E G YN+ V+KDG V+ KH+LP+ VFD
Sbjct 65 DAMAAMAALAAQVVDGPALGIGGPYVDETGS-YNAWWVLKDGRVIARALKHHLPHDDVFD 123
Query 122 EKRYFSPGHQHLVFEYLGHKFGVLICEDIWSINTVKQLSKLNVETVLVLNASPYEVGKPQ 181
E R F G GV +CED W + L+ E ++V N SPY GK
Sbjct 124 EMRLFDQGPVSDPLRLGPVALGVPVCEDAWHPDVAGALAAAGAEVLMVPNGSPYRRGKLD 183
Query 182 HRVQTLTELSKQLNVHLVYLNQVGGQDDLIFDGSSFIINHDGEVAFQAPSFKEELYYSEF 241
R Q + + L+YLN VGGQDD +FDG+SF++N DG VA Q P+F+E + + +
Sbjct 184 LRRQVTGARVAETGLPLLYLNMVGGQDDQLFDGASFVLNPDGSVAVQLPAFEEAVVHVDL 243
Query 242 DIEQKRYKKIDP---APALDTIAEIYQSLVMATRDYVQRSGFSGVILGLSGGIDSALTLA 298
+ ++ + AP D I + Y+++V+ +DY+++SGFS V+LGLSGGIDSAL
Sbjct 244 ERGAADWRAVPADIVAPPGD-IEQDYRAMVLGLQDYLRKSGFSRVVLGLSGGIDSALVAV 302
Query 299 IAADAIGADKVQAVMMPYTYTSQISVEDATEQARRMGVTFGIAEIHPIVNSFMQTLYPFF 358
IAADA+GA V VM+P YTSQ S++DA + ARR+G EI + L
Sbjct 303 IAADALGAGNVHCVMLPSRYTSQGSLDDAADLARRLGARLDTVEIEGPRAAVEGALAHVL 362
Query 359 GNAPADATEENLQARARGTLLMGLSNKFGNLVLSTGNKSELAVGYCTLYGDMVGGFAVLK 418
D TEEN+Q+R RG +LM +SNKFG ++L+TGNKSE+AVGYCT+YGDM GG+ LK
Sbjct 363 AGTAPDVTEENIQSRLRGVILMAISNKFGAMLLTTGNKSEVAVGYCTIYGDMAGGYNPLK 422
Query 419 DVYKTIVFELAKYRNTLSET-------PVIPERVITRPPSAELRPDQKDQDSLPAYDILD 471
D+YKT VFE ++RN +IP +I +PPSAELR +Q DQDSLP Y++LD
Sbjct 423 DLYKTRVFETCRWRNATHRPWMQAPAGEIIPVAIIDKPPSAELRENQTDQDSLPPYEVLD 482
Query 472 AILYAYIEEDQSQSDIIAKGFDKEVVEKVIRLVDRNEYKRRQGAIGPRISSRAFSRERRY 531
AIL +E DQS I+A GFD+ V+++ L+ +E+KR Q A GPR+++RAF +RRY
Sbjct 483 AILERLVEGDQSVDQIVAAGFDRATVKRIEHLLYISEWKRFQSAPGPRLTTRAFWLDRRY 542
Query 532 PIVNGWR 538
P+VN WR
Sbjct 543 PMVNRWR 549
>Q8KEX2 NH(3)-dependent NAD(+) synthetase [Chlorobaculum tepidum
(strain ATCC 49652 / DSM 12025 / NBRC 103806 / TLS)]
Length=277
Score = 157 bits (396), Expect = 4e-47
Identities = 99/269 (37%), Positives = 152/269 (57%), Gaps = 20/269 (7%)
Query 267 LVMATRDYVQRSGFSGVILGLSGGIDSALTLAIAADAIGADKVQAVMMPYTYTSQISVED 326
LV R+ +++ GF V+LGLSGGIDSA+ +A A+G + V A+MMPY +SQ S++
Sbjct 17 LVPFIRNEIRKFGFGSVVLGLSGGIDSAVVCELAVRALGVENVLALMMPYKTSSQESLDH 76
Query 327 ATEQARRMGVTFGIAEIHPIVNSFMQTLYPFFGNAPADAT---EENLQARARGTLLMGLS 383
A R+G+ + I + +V++F T DA+ N+ AR+R L +S
Sbjct 77 AELMVDRLGIRYEIMPVTEVVDAFFAT--------RPDASRLRRGNVMARSRMLCLYDVS 128
Query 384 NKFGNLVLSTGNKSELAVGYCTLYGDMVGGFAVLKDVYKTIVFELAKYRNTLSETPVIPE 443
+ G LVL T NK+EL +GY T++GDM + D+YKT +F LA++ IP
Sbjct 129 ARDGCLVLGTSNKTELMLGYGTMFGDMASAVNPIGDLYKTQIFGLARHLG-------IPA 181
Query 444 RVITRPPSAELRPDQKDQDSLP-AYDILDAILYAYIEEDQSQSDIIAKGFDKEVVEKVIR 502
+I +PPSA+L Q D+ L +Y+ +D +LY +EE + I+A+G D ++V
Sbjct 182 PLIDKPPSADLWEGQSDEADLGFSYEEVDQLLYMMLEERMDRDAILAEGIDSAFYQRVRS 241
Query 503 LVDRNEYKRRQGAIGPRISSRAFSRERRY 531
+V RN+YKR I ++SSR + RY
Sbjct 242 MVVRNQYKRMMPVIA-KLSSRTPGIDFRY 269
>A5G7Y9 NH(3)-dependent NAD(+) synthetase [Geotalea uraniireducens
(strain Rf4)]
Length=273
Score = 152 bits (383), Expect = 3e-45
Identities = 96/266 (36%), Positives = 147/266 (55%), Gaps = 14/266 (5%)
Query 267 LVMATRDYVQRSGFSGVILGLSGGIDSALTLAIAADAIGADKVQAVMMPYTYTSQISVED 326
LV R+ V++ G +LGLSGGIDSAL +AA+A+G + V A MPY ++ S
Sbjct 15 LVGFIREEVRKIGLQKAVLGLSGGIDSALVAYLAAEALGPENVYACTMPYRTSNPESEAH 74
Query 327 ATEQARRMGVTFGIAEIHPIVNSFMQTLYPFFGNAPADATEENLQARARGTLLMGLSNKF 386
A A R+G+ + + EI P+V+++ Q + P N N AR R T+L S +
Sbjct 75 ARLVAERLGINYRVIEITPMVDAYFQ-MVPDADN----MRRGNKMARERMTILYDHSAAY 129
Query 387 GNLVLSTGNKSELAVGYCTLYGDMVGGFAVLKDVYKTIVFELAKYRNTLSETPVIPERVI 446
LVL T NK+EL +GY TL+GDM + D+YKT V++ LSE +P VI
Sbjct 130 SALVLGTSNKTELLLGYGTLFGDMASALNPIGDIYKTQVWQ-------LSEAMGVPREVI 182
Query 447 TRPPSAELRPDQKDQDSLP-AYDILDAILYAYIEEDQSQSDIIAKGFDKEVVEKVIRLVD 505
+ PSA+L Q D+ L Y +D +LY ++ ++ ++IA+GF + ++K+ V
Sbjct 183 EKKPSADLWAGQTDEQELGFTYREVDELLYQMVDLRCNRQELIAQGFSADFIDKIYGKVQ 242
Query 506 RNEYKRRQGAIGPRISSRAFSRERRY 531
+ +KRR I ++S+R R+ RY
Sbjct 243 NSHFKRRLPVIA-KVSTRTIDRDFRY 267
Score = 18.9 bits (37), Expect = 7.7
Identities = 10/31 (32%), Positives = 15/31 (48%), Gaps = 0/31 (0%)
Query 234 EELYYSEFDIEQKRYKKIDPAPALDTIAEIY 264
+EL Y D+ R + I + D I +IY
Sbjct 208 DELLYQMVDLRCNRQELIAQGFSADFIDKIY 238
>B3ELE3 NH(3)-dependent NAD(+) synthetase [Chlorobium phaeobacteroides
(strain BS1)]
Length=277
Score = 152 bits (383), Expect = 3e-45
Identities = 96/266 (36%), Positives = 148/266 (56%), Gaps = 14/266 (5%)
Query 267 LVMATRDYVQRSGFSGVILGLSGGIDSALTLAIAADAIGADKVQAVMMPYTYTSQISVED 326
L+ R+ +++ GF + GLSGGIDSA+ +A A+G + V AV+MPY +S SV D
Sbjct 17 LLAFIRNEIRKFGFRSAVFGLSGGIDSAVVCELAKRALGPENVLAVLMPYRTSSPESVND 76
Query 327 ATEQARRMGVTFGIAEIHPIVNSFMQTLYPFFGNAPADATEENLQARARGTLLMGLSNKF 386
A + GV EI +V++F + + + N+ ARAR L +S +
Sbjct 77 ARLMVEKTGVRSEEVEITGVVDAFFDGV-----SGAGNLRMGNVMARARMLYLYDISARD 131
Query 387 GNLVLSTGNKSELAVGYCTLYGDMVGGFAVLKDVYKTIVFELAKYRNTLSETPVIPERVI 446
G LV+ T NK+EL +GY TL+GDM + D+YKT ++ LA++ IPE ++
Sbjct 132 GRLVIGTSNKTELLLGYGTLFGDMASAVNPIGDLYKTQLWGLARHLQ-------IPEELV 184
Query 447 TRPPSAELRPDQKDQDSLP-AYDILDAILYAYIEEDQSQSDIIAKGFDKEVVEKVIRLVD 505
++ PSA+L Q D+ L Y +D +LY +E+ S+SDII G ++ +KV ++V
Sbjct 185 SKIPSADLWEGQSDEADLGFGYGEVDLLLYMMLEKRMSRSDIIEAGIEESFYDKVRKMVV 244
Query 506 RNEYKRRQGAIGPRISSRAFSRERRY 531
RN+YKR I +IS+R + RY
Sbjct 245 RNQYKRMMPVIA-KISARTPGIDFRY 269
Score = 20.8 bits (42), Expect = 1.9
Identities = 10/20 (50%), Positives = 12/20 (60%), Gaps = 0/20 (0%)
Query 49 GYPAEDLLLRPNLNKRMQKA 68
GY DLLL L KRM ++
Sbjct 205 GYGEVDLLLYMMLEKRMSRS 224
>B3E4K8 NH(3)-dependent NAD(+) synthetase [Trichlorobacter lovleyi
(strain ATCC BAA-1151 / DSM 17278 / SZ)]
Length=273
Score = 150 bits (379), Expect = 9e-45
Identities = 96/270 (36%), Positives = 145/270 (54%), Gaps = 14/270 (5%)
Query 263 IYQSLVMATRDYVQRSGFSGVILGLSGGIDSALTLAIAADAIGADKVQAVMMPYTYTSQI 322
+ + LV +D + + G +LGLSGGIDSAL +AA+A+G + V A+ MPY ++
Sbjct 11 VRRMLVGFLQDEIWKVGAKKAVLGLSGGIDSALVCHLAAEALGPENVHAICMPYRTSNPE 70
Query 323 SVEDATEQARRMGVTFGIAEIHPIVNSFMQTLYPFFGNAPADATEENLQARARGTLLMGL 382
S A A GV F + I P+V+++ +P N N AR R T+L
Sbjct 71 SEAHARLVAEASGVQFSVVGITPMVDAYFDQ-FPDANN----MRRGNKMARERMTVLFDH 125
Query 383 SNKFGNLVLSTGNKSELAVGYCTLYGDMVGGFAVLKDVYKTIVFELAKYRNTLSETPVIP 442
S +G LVL T NK+EL +GY TLYGDM + D+YKT V++ LSE +P
Sbjct 126 SALYGGLVLGTSNKTELLLGYGTLYGDMASALNPIGDLYKTQVWQ-------LSEEVGVP 178
Query 443 ERVITRPPSAELRPDQKDQDSLP-AYDILDAILYAYIEEDQSQSDIIAKGFDKEVVEKVI 501
+ VI + PSA+L Q D++ L Y +D +LY +++ ++++A GF +E V +
Sbjct 179 KPVIEKKPSADLWAGQTDEEELGFTYREVDELLYRMVDQRADTAELVAAGFKQEFVSSIY 238
Query 502 RLVDRNEYKRRQGAIGPRISSRAFSRERRY 531
V + +KRR I ++S R R+ RY
Sbjct 239 SKVQNSHFKRRLPVIA-KVSGRTIDRDFRY 267
>P9WJJ3 Glutamine-dependent NAD(+) synthetase [Mycobacterium tuberculosis
(strain ATCC 25618 / H37Rv)]
Length=679
Score = 129 bits (324), Expect = 9e-35
Identities = 132/508 (26%), Positives = 212/508 (42%), Gaps = 82/508 (16%)
Query 5 KIALAQFSPHIGNIDSNAQRMVEQANEAKKQNADLIIFPELSVIGYPAEDLLLRPNLNKR 64
++A IG+ +NA +++ A A L +FPEL++ GY ED+LL+ +L
Sbjct 13 RVAACTHHTTIGDPAANAASVLDMARACHDDGAALAVFPELTLSGYSIEDVLLQDSLLDA 72
Query 65 MQKAFQQL-KEVKDIVMVFGFVHQTEEGHR-YNSAAVMKDGVVLGVYNKHNLPNYSVFDE 122
++ A L E D++ V HR YN+A V+ G VLGV K LP Y F E
Sbjct 73 VEDALLDLVTESADLLPVLVVGAPLRHRHRIYNTAVVIHRGAVLGVVPKSYLPTYREFYE 132
Query 123 KRYFSPGHQH----------------LVF---EYLGHKFGVLICEDIWSINTVKQLSKLN 163
+R +PG L+F + G V ICED++ + L
Sbjct 133 RRQMAPGDGERGTIRIGGADVAFGTDLLFAASDLPGFVLHVEICEDMFVPMPPSAEAALA 192
Query 164 VETVLV-LNASPYEVGKPQHRVQTLTELSKQLNVHLVYLNQVGGQD--DLIFDGSSFIIN 220
TVL L+ SP +G+ + R S + VY G+ DL +DG + I
Sbjct 193 GATVLANLSGSPITIGRAEDRRLLARSASARCLAAYVYAAAGEGESTTDLAWDGQTMIWE 252
Query 221 HDGEVAFQAPSF-----------------KEELYYSEFDIEQKRYKKI------------ 251
+ G + ++ F E L FD ++ ++++
Sbjct 253 N-GALLAESERFPKGVRRSVADVDTELLRSERLRMGTFDDNRRHHRELTESFRRIDFALD 311
Query 252 --------------------DPAPALDTIAEIYQSLVMATRDYVQRSGFSGVILGLSGGI 291
DP E Y V ++ + V++G+SGG+
Sbjct 312 PPAGDIGLLREVERFPFVPADPQRLQQDCYEAYNIQVSGLEQRLRALDYPKVVIGVSGGL 371
Query 292 DSALTLAIAADAIGAD-----KVQAVMMPYTYTSQISVEDATEQARRMGVTFGIAEIHPI 346
DS L +A A+ + + A +P T + + +A + AR +GVTF +I
Sbjct 372 DSTHALIVATHAMDREGRPRSDILAFALPGFATGEHTKNNAIKLARALGVTFSEIDIGDT 431
Query 347 VNSFMQTL-YPF-FGNAPADATEENLQARARGTLLMGLSNKFGNLVLSTGNKSELAVGYC 404
+ T+ +P+ G D T EN+QA R L ++N+ G +VL TG+ SELA+G+
Sbjct 432 ARLMLHTIGHPYSVGEKVYDVTFENVQAGLRTDYLFRIANQRGGIVLGTGDLSELALGWS 491
Query 405 TL-YGDMVGGFAVLKDVYKTIVFELAKY 431
T GD + + V V KT++ L ++
Sbjct 492 TYGVGDQMSHYNVNAGVPKTLIQHLIRW 519
Score = 18.9 bits (37), Expect = 8.5
Identities = 9/26 (35%), Positives = 12/26 (46%), Gaps = 0/26 (0%)
Query 444 RVITRPPSAELRPDQKDQDSLPAYDI 469
R + R P P + QD AY+I
Sbjct 321 REVERFPFVPADPQRLQQDCYEAYNI 346
>O25096 NH(3)-dependent NAD(+) synthetase [Helicobacter pylori
(strain ATCC 700392 / 26695)]
Length=260
Score = 104 bits (259), Expect = 3e-28
Identities = 77/267 (29%), Positives = 133/267 (50%), Gaps = 22/267 (8%)
Query 264 YQSLVMATRDY----VQRSGFSGVILGLSGGIDSALTLAIAADAIGADKVQAVMMPYTYT 319
YQ L++ D+ VQ+ GF V+ GLSGG+DSA+ + + + + A++MP + +
Sbjct 5 YQKLIVYLCDFLEKEVQKRGFKKVVYGLSGGLDSAV-VGVLCQKVFKENAHALLMPSSVS 63
Query 320 SQISVEDATEQARRMGVTFGIAEIHPIVNSFMQTLYPFFGNAPADATEENLQARARGTLL 379
+ DA + + + I P F F +A + N AR R L
Sbjct 64 MPENKTDALNLCEKFSIPYTEYSIAPYDAIFSS----HFKDASL-TRKGNFCARLRMAFL 118
Query 380 MGLSNKFGNLVLSTGNKSELAVGYCTLYGDMVGGFAVLKDVYKTIVFELAKYRNTLSETP 439
S K +LV+ T NKSE +GY TL+GD+ + +++KT V+ELA+ N
Sbjct 119 YDYSLKSDSLVIGTSNKSERMLGYGTLFGDLACAINPIGELFKTEVYELARRLN------ 172
Query 440 VIPERVITRPPSAELRPDQKDQDSLP-AYDILDAIL---YAYIEEDQSQSDIIAK-GFDK 494
IP++++ +PPSA+L Q D+ L Y ++D +L A + ++ +A+ G+D+
Sbjct 173 -IPKKILNKPPSADLFVGQSDEKDLGYPYSVIDPLLKDIEALFQTKPIDTETLAQLGYDE 231
Query 495 EVVEKVIRLVDRNEYKRRQGAIGPRIS 521
+V+ + + +N +K AI R +
Sbjct 232 ILVKNITSRIQKNAFKLELPAIAKRFN 258
>Q75SP7 (R)-stereoselective amidase [Pseudomonas sp]
Length=274
Score = 84.0 bits (206), Expect = 4e-21
Identities = 73/253 (29%), Positives = 121/253 (48%), Gaps = 16/253 (6%)
Query 5 KIALAQFSPHIGNIDSNAQRMVEQANEAKKQNADLIIFPELSVIGYPAEDLLLR---PNL 61
KI L Q + G+ N R + A + DL++FPE + G+ L + P
Sbjct 2 KIELVQLAGRDGDTAYNLSRTLN-AIATCAGDTDLLVFPETYLSGFVGGAQLAQVAEPLH 60
Query 62 NKRMQKAFQQLKEVKDIVMVFGFVHQTEEGHRYNSAA-VMKDGVVLGVYNKHNLPNYSVF 120
+Q Q +++ +D+ +V GF + +G YNS+ V +G+ L H P+
Sbjct 61 GTTLQTLLQAVRQ-RDVAVVLGFA-EVHQGRFYNSSVLVTPEGIALQYRKTHLWPS---- 114
Query 121 DEKRYFSPGHQHLVFEYLGHKFGVLICEDIWSINTVKQLSKLNVETVLVLNASPYEVGKP 180
E+ FSPG + + G + G+LIC DI T + L++L E V+V N + G P
Sbjct 115 -ERSDFSPGDRFTTVLWRGVRVGLLICYDIELPETSRALAQLGAEVVIVTNGNMDPYG-P 172
Query 181 QHRVQTLTELSKQLNVHLVYLNQVG-GQDDLIFDGSSFIINHDGEVAFQAPSFKEELYYS 239
HR + +++ + V +N+VG G D L+F G S ++ G V F+A E +
Sbjct 173 VHRTAIMAR-AQENQLFAVMVNRVGAGDDGLVFAGGSMAVDPFGRVLFEA-GRDEVRHVV 230
Query 240 EFDIEQKRYKKID 252
E D++Q + + D
Sbjct 231 ELDLDQLKAARRD 243
>Q9UYV8 Nitrilase [Pyrococcus abyssi (strain GE5 / Orsay)]
Length=262
Score = 67.8 bits (164), Expect = 1e-15
Identities = 68/260 (26%), Positives = 113/260 (43%), Gaps = 28/260 (11%)
Query 5 KIALAQFSPHIGNIDSNAQRMVEQANEAKKQNADLIIFPELSVIGYPAEDLLLRPNLNKR 64
K+A Q +P I D N + + EA KQ A L++ PEL GY E +
Sbjct 3 KVAYVQMNPQILEPDKNYSKAEKLIKEASKQGAQLVVLPELFDTGYNFE-------TREE 55
Query 65 MQKAFQQLKEVKDIVMVFGFVHQT----------EEGH-RYNSAAVMKDGVVLGVYNKHN 113
+ + Q++ E + + T ++G YNSA V+ +G Y K +
Sbjct 56 VFEIAQKIPEGETTTFLMDVARDTGVYIVAGTAEKDGDVLYNSAVVVGPRGFIGKYRKIH 115
Query 114 LPNYSVFDEKRYFSPGHQHL-VFEYLGHKFGVLICEDIWSINTVKQLSKLNVETVLVLNA 172
L + EK +F PG VF+ K GV+IC D + + + L+ + + A
Sbjct 116 L----FYREKFFFEPGDLGFRVFDLGFMKVGVMICFDWFFPESARTLALKGADVI----A 167
Query 173 SPYEVGKPQHRVQTLTELSKQLNVHLVYLNQVGGQDDLIFDGSSFIINHDGEVAFQAPSF 232
P + P + + + + + V+ V ++VG + L F G S I + EV A
Sbjct 168 HPANLVMP-YAPRAMPIRALENKVYTVTADRVGEERGLKFIGKSLIASPKAEVLSMASET 226
Query 233 KEELYYSEFDIEQKRYKKID 252
+EE+ +E D+ R K+I+
Sbjct 227 EEEVGVAEIDLYLVRNKRIN 246
>Q81RP3 NH(3)-dependent NAD(+) synthetase [Bacillus anthracis]
Length=272
Score = 66.6 bits (161), Expect = 3e-15
Identities = 75/282 (27%), Positives = 125/282 (44%), Gaps = 35/282 (12%)
Query 243 IEQKRYKKIDPAPALDTIAEIYQSLVMATRDYVQRSGFSGVILGLSGGIDSALTLAIAAD 302
++++ K + P +D AEI + V +DYV+++G G +LG+SGG DS L +A
Sbjct 3 LQEQIMKALHVQPVIDPKAEI-RKRVDFLKDYVKKTGAKGFVLGISGGQDSTLAGRLAQL 61
Query 303 AI-------GADKVQAVMMPYTYTSQISVEDATEQARRMGVTFGIA-EIHPIVNSFMQTL 354
A+ G AV +PY Q +DA + + +A +I V++F
Sbjct 62 AVEEIRNEGGNATFIAVRLPYKV--QKDEDDAQLALQFIQADQSVAFDIASTVDAFSNQY 119
Query 355 YPFFGNAPADATEENLQARARGTLLMGLSNKFGNLVLSTGNKSELAVGYCTLYGDMVGGF 414
+ D + N++AR R + + G LV+ T + +E G+ T +GD GG
Sbjct 120 ENLLDESLTDFNKGNVKARIRMVTQYAIGGQKGLLVIGTDHAAEAVTGFFTKFGD--GGA 177
Query 415 AVLKDVYKTIVFELAKYRNTLSETPVIPERVITRPPSAEL---RPDQKDQDSLP-AYDIL 470
+L T + L + ER+ + P+A+L +P Q D+ L YD L
Sbjct 178 DLLPLTGLT-----KRQGRALLQELGADERLYLKMPTADLLDEKPGQADETELGITYDQL 232
Query 471 DAILYAYIEEDQSQSDIIAKGFDKEVVEKVIRLVDRNEYKRR 512
D Y+E K +V EK+ + +E+KR+
Sbjct 233 D----DYLE---------GKTVPADVAEKIEKRYTVSEHKRQ 261
>P08164 NH(3)-dependent NAD(+) synthetase [Bacillus subtilis (strain
168)]
Length=272
Score = 66.2 bits (160), Expect = 4e-15
Identities = 66/243 (27%), Positives = 113/243 (47%), Gaps = 20/243 (8%)
Query 243 IEQKRYKKIDPAPALDTIAEIYQSLVMATRDYVQRSGFSGVILGLSGGIDSALTLAIAAD 302
+++K +++ P++D EI + V + YV+++G G +LG+SGG DS L +A
Sbjct 3 MQEKIMRELHVKPSIDPKQEI-EDRVNFLKQYVKKTGAKGFVLGISGGQDSTLAGRLAQL 61
Query 303 AI------GADKVQAVMMPYTYTSQISVEDATEQARRMGVTFGIA-EIHPIVNSFMQTLY 355
A+ G D Q + + + +Q +DA + + +I V++F
Sbjct 62 AVESIREEGGD-AQFIAVRLPHGTQQDEDDAQLALKFIKPDKSWKFDIKSTVSAFSDQYQ 120
Query 356 PFFGNAPADATEENLQARARGTLLMGLSNKFGNLVLSTGNKSELAVGYCTLYGDMVGGFA 415
G+ D + N++AR R + + G LVL T + +E G+ T YGD GG
Sbjct 121 QETGDQLTDFNKGNVKARTRMIAQYAIGGQEGLLVLGTDHAAEAVTGFFTKYGD--GGAD 178
Query 416 VLKDVYKTIVFELAKYRNTLSETPVIPERVITRPPSAEL---RPDQKDQDSLP-AYDILD 471
+L T + TL + PER+ + P+A+L +P Q D+ L +YD +D
Sbjct 179 LLPLTGLT-----KRQGRTLLKELGAPERLYLKEPTADLLDEKPQQSDETELGISYDEID 233
Query 472 AIL 474
L
Sbjct 234 DYL 236
>Q6IA69 Glutamine-dependent NAD(+) synthetase [Homo sapiens]
Length=706
Score = 57.0 bits (136), Expect = 1e-11
Identities = 114/564 (20%), Positives = 199/564 (35%), Gaps = 128/564 (23%)
Query 17 NIDSNAQRMVEQANEAKKQNADLIIFPELSVIGYPAEDLLLRPNLNKRMQKAFQQLKE-- 74
+ + N QR+++ AK + A + PEL + GY D + + L E
Sbjct 18 DFEGNLQRILKSIEIAKNRGARYRLGPELEICGYGCWDHYYESDTLLHSFQVLAALVESP 77
Query 75 -VKDIVMVFGF--VHQTEEGHRYNSAAVMKDGVVLGVYNKHNLPNYSVFDEKRYFSPGHQ 131
+DI+ G +H+ RYN + + +L + K L N + E R+F+P +
Sbjct 78 VTQDIICDVGMPVMHRNV---RYNCRVIFLNRKILLIRPKMALANEGNYRELRWFTPWSR 134
Query 132 --HLVFEYLGHKF-----------------------GVLICEDIWSINTVK-QLSKLNVE 165
H +L G ICE++W+ ++ + VE
Sbjct 135 SRHTEEYFLPRMIQDLTKQETVPFGDAVLVTWDTCIGSEICEELWTPHSPHIDMGLDGVE 194
Query 166 TVLVLNASPYEVGKPQHRVQTLTELSKQLNVHLVYLNQVGGQDDLIFDGSSFIINHDGEV 225
+ + S + K RV +T ++ + + NQ G D ++ +I +G V
Sbjct 195 IITNASGSHQVLRKANTRVDLVTMVTSKNGGIYLLANQKGCDGDRLYYDGCAMIAMNGSV 254
Query 226 AFQAPSFK---EELYYSEFDIEQKRYKKID--------------PAPALDTIAEIYQSLV 268
Q F E+ + D+E R + + P +D ++ L+
Sbjct 255 FAQGSQFSLDDVEVLTATLDLEDVRSYRAEISSRNLAASRASPYPRVKVDFALSCHEDLL 314
Query 269 MATR-----------------------DYVQRSGFSGVILGLSGGIDSALTLAI------ 299
D+++RS +G +L LSGG+DSA T +
Sbjct 315 APISEPIEWKYHSPEEEISLGPACWLWDFLRRSQQAGFLLPLSGGVDSAATACLIYSMCC 374
Query 300 -AADAIGADK------VQAVMMPYTYT----------------------SQISVEDATEQ 330
+A+ + V+ ++ +YT SQ + A E
Sbjct 375 QVCEAVRSGNEEVLADVRTIVNQISYTPQDPRDLCGRILTTCYMASKNSSQETCTRAREL 434
Query 331 ARRMGVTFGIAEIHPIVNSFMQTLYPFFGNAPADATE----------ENLQARARGTL-- 378
A+++G I P V + M G +P A +N+QAR R L
Sbjct 435 AQQIGSHHISLNIDPAVKAVMGIFSLVTGKSPLFAAHGGSSRENLALQNVQARIRMVLAY 494
Query 379 ------LMGLSNKFGNLVLSTGNKSELAVGYCTLYGDMVGGFAVLKDVYKTIVFELAKYR 432
L G LVL + N E +GY T Y + + KT + ++
Sbjct 495 LFAQLSLWSRGVHGGLLVLGSANVDESLLGYLTKYDCSSADINPIGGISKTDLRAFVQFC 554
Query 433 NTLSETPVIPERVITRPPSAELRP 456
+ P + + ++ P +AEL P
Sbjct 555 IQRFQLPAL-QSILLAPATAELEP 577
>Q9VYA0 Glutamine-dependent NAD(+) synthetase [Drosophila melanogaster]
Length=787
Score = 55.8 bits (133), Expect = 3e-11
Identities = 109/576 (19%), Positives = 205/576 (36%), Gaps = 122/576 (21%)
Query 6 IALAQFSPHIGNIDSNAQRMVEQANEAKKQNADLIIFPELSVIGYPAEDLLLRPNLNKRM 65
+A++ + + + N R+++ EAK A PEL V GY ED P+
Sbjct 7 VAVSTLNQWALDFEGNMVRILQSILEAKDMGASYRTGPELEVCGYSCEDHFREPDTFLHS 66
Query 66 QKAFQQ--LKEVKDIVMVFGFVHQTEEGHRYNSAAVMKDGVVLGVYNKHNLPNYSVFDEK 123
+ + + + + ++V + YN + +L + K + + + E
Sbjct 67 WEVLLEVMMSPMCENMLVDVGMPVMHRNVAYNCRVAFFNRQILLIRPKMAMCDDGNYRES 126
Query 124 RYFSPGHQHL----------VFEYLGHK---------------FGVLICEDIWSINTVK- 157
R+F+ + L + ++ G + G ICE++W++ +
Sbjct 127 RWFTAWTKALQTEEYVLPRMIAQHTGQQTVPFGDAVIATRDTCLGYEICEELWNVRSKHI 186
Query 158 QLSKLNVETVLVLNASPYEVGKPQHRVQTLTELSKQLNVHLVYLNQVGGQDDLIFDGSSF 217
++S VE ++ + S E+ K + S + ++ N G ++
Sbjct 187 EMSLAGVELIVNSSGSYMELRKAHITSDLIRNASFKAGGAYLFSNLRGCDGQRVYFNGCS 246
Query 218 IINHDGEVAFQAPSFK---EELYYSEFDIEQKR------------------YKKIDPAPA 256
I +GE+ ++ F E+ + D+E+ R Y +I
Sbjct 247 AIALNGEILARSQQFALQDVEVTLATIDLEEIRAYRVSLRSRCTAAASAAEYPRIHCDFE 306
Query 257 LDTIAEIYQSLVMATR-------------------DYVQRSGFSGVILGLSGGIDSALTL 297
+ T ++I+++ DY++RSG G L LSGG+DS+ +
Sbjct 307 MSTHSDIFKTSTPPLNWPMHTPEEEIALGPACWLWDYLRRSGQGGFFLPLSGGVDSSSSA 366
Query 298 AIA-------------ADAIGADKVQAVMMPYTYT------------------SQISVED 326
I DA ++ ++ YT S S ++
Sbjct 367 TIVHSMCRQIVQAVQQGDAQVLHDIRQLLADSDYTPDNAAGLCNRLLVTCYMGSVNSSKE 426
Query 327 ATEQARRMGVTFGIAEIHPIVNSFMQTLYPFF--------------GNAPADATEENLQA 372
+A ++ G I ++S + L F G A + +N+Q+
Sbjct 427 TRRRAAQLANQLGSYHIEISIDSAVNALLSIFNAVTGLTPRFRTQGGCARQNLALQNMQS 486
Query 373 RAR-------GTLLMGLSNKFGNL-VLSTGNKSELAVGYCTLYGDMVGGFAVLKDVYKTI 424
R R L + + N+ G L VL + N E GY T Y + + K
Sbjct 487 RIRMVLAYIFAQLTLWVRNRPGGLLVLGSANVDESLRGYLTKYDCSSADINPIGGISKMD 546
Query 425 VFELAKYRNTLSETPVIPERVITRPPSAELRPDQKD 460
+ Y PV+ E +I PP+AEL P Q++
Sbjct 547 LRRFLTYAKDKFNLPVL-ESIIDAPPTAELEPLQEN 581
>Q711T7 Glutamine-dependent NAD(+) synthetase [Mus musculus]
Length=725
Score = 55.1 bits (131), Expect = 5e-11
Identities = 113/567 (20%), Positives = 208/567 (37%), Gaps = 134/567 (24%)
Query 17 NIDSNAQRMVEQANEAKKQNADLIIFPELSVIGYPA------EDLLLRPNLNKRMQKAFQ 70
+ + N QR+++ AK + A + PEL + GY D LL + ++ A
Sbjct 18 DFEGNFQRILKSIQIAKGKGARYRLGPELEICGYGCWDHYHESDTLLH---SLQVLAALL 74
Query 71 QLKEVKDIVMVFGF--VHQTEEGHRYNSAAVMKDGVVLGVYNKHNLPNYSVFDEKRYFSP 128
+DI+ G +H+ RYN + + +L + K L N + E R+F+P
Sbjct 75 DSPVTQDIICDVGMPIMHRNV---RYNCRVIFLNRKILLIRPKMALANEGNYRELRWFTP 131
Query 129 G-HQHLVFEYLGHKF------------------------GVLICEDIWSINTVK-QLSKL 162
EY+ + G ICE++W+ + +
Sbjct 132 WTRSRQTEEYVLPRMLQDLTKQKTVPFGDVVLATQDTCVGSEICEELWTPRSPHIDMGLD 191
Query 163 NVETVLVLNASPYEVGKPQHRVQTLTELSKQLNVHLVYLNQVGGQDDLIFDGSSFIINHD 222
VE + + S + + K RV +T + + + NQ G D ++ +I +
Sbjct 192 GVEIITNASGSHHVLRKAHTRVDLVTMATSKNGGIYLLANQKGCDGDRLYYDGCAMIAMN 251
Query 223 GEVAFQAPSFK---EELYYSEFDIEQKR-YK-----------KIDPAP--ALDTIAEIYQ 265
G + Q F E+ + D+E R YK ++ P P +D + +
Sbjct 252 GSIFAQGTQFSLDDVEVLTATLDLEDVRSYKAEISSRNLEATRVSPYPRVTVDFALSVSE 311
Query 266 SLVMATR-----------------------DYVQRSGFSGVILGLSGGIDSALTLAI--- 299
L+ D+++RS +G L LSGG+DSA + I
Sbjct 312 DLLEPVSEPMEWTYHRPEEEISLGPACWLWDFLRRSKQAGFFLPLSGGVDSAASACIVYS 371
Query 300 ----AADAIGADK------VQAVMMPYTYT------------------SQISVEDATEQA 331
DA+ + VQ ++ +YT S+ S ++ +A
Sbjct 372 MCCLVCDAVKSGNQQVLTDVQNLVDESSYTPQDPRELCGRLLTTCYMASENSSQETHSRA 431
Query 332 RRMGVTFGIAEIHPIVNSFMQTLYPFF--------------GNAPADATEENLQARARGT 377
++ G I+ +++ ++ + F G++ + +N+QAR R
Sbjct 432 TKLAQLIGSYHINLSIDTAVKAVLGIFSLMTGKLPRFSAHGGSSRENLALQNVQARIRMV 491
Query 378 L--------LMGLSNKFGNLVLSTGNKSELAVGYCTLYGDMVGGFAVLKDVYKTIVFELA 429
L L + LVL + N E +GY T Y + + KT +
Sbjct 492 LAYLFAQLSLWSRGARGSLLVLGSANVDESLLGYLTKYDCSSADINPIGGISKTDLRAFV 551
Query 430 KYRNTLSETPVIPERVITRPPSAELRP 456
++ + PV+ + +++ P +AEL P
Sbjct 552 QFCAERFQLPVL-QTILSAPATAELEP 577
Lambda K H a alpha
0.320 0.137 0.392 0.792 4.96
Gapped
Lambda K H a alpha sigma
0.267 0.0410 0.140 1.90 42.6 43.6
Effective search space used: 3735152
Database: ca96eaa63c35b328804186b38321af07.SwissProt.fasta
Posted date: May 12, 2024 7:19 PM
Number of letters in database: 8,680
Number of sequences in database: 19
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 11
Window for multiple hits: 40
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Data ...
