BLASTP 2.12.0+
Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics: Alejandro A. Schaffer,
L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri
I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids
Res. 29:2994-3005.
Database: a8153455ce498e0a073dedb0b9d2e8aa.SwissProt.fasta
14 sequences; 3,160 total letters
Query= ACIAD0159
Length=228
Score E
Sequences producing significant alignments: (Bits) Value
P40582 Glutathione S-transferase 1 [Saccharomyces cerevisiae (str... 143 6e-46
Q9P6M1 Glutathione S-transferase 3 [Schizosaccharomyces pombe (st... 132 2e-41
Q9Y7Q2 Glutathione S-transferase 1 [Schizosaccharomyces pombe (st... 52.8 5e-12
Q52828 Protein GstA [Rhizobium leguminosarum] 43.1 9e-09
P30347 Protein LigF [Sphingobium sp. (strain NBRC 103272 / SYK-6)] 43.1 1e-08
Q9SR36 Glutathione S-transferase U8 [Arabidopsis thaliana] 42.7 1e-08
Q4WQZ2 Glutathione S-transferase-like protein tpcF [Aspergillus f... 42.7 1e-08
Q9ZW29 Glutathione S-transferase U2 [Arabidopsis thaliana] 41.2 5e-08
B3FWR8 Glutathione S-transferase hmp2 [Hypomyces subiculosus] 38.9 3e-07
Q9CA57 Glutathione S-transferase U10 [Arabidopsis thaliana] 38.5 4e-07
P46417 Glutathione S-transferase 3 [Glycine max] 38.1 5e-07
Q9ZW30 Glutathione S-transferase U1 [Arabidopsis thaliana] 37.7 7e-07
P46430 Glutathione S-transferase 1 [Manduca sexta] 37.4 9e-07
P32111 Probable glutathione S-transferase [Solanum tuberosum] 37.4 9e-07
>P40582 Glutathione S-transferase 1 [Saccharomyces cerevisiae
(strain ATCC 204508 / S288c)]
Length=234
Score = 143 bits (360), Expect = 6e-46
Identities = 79/222 (36%), Positives = 127/222 (57%), Gaps = 12/222 (5%)
Query 15 MLTLHHLEQSRSFRILWALEELQQNYEIKYYKRSPKFAAPPELKQVHPLGKAPILQ---- 70
++ +H L+ SR+FR+LW L+ L YEI YKR F APPELK++HPLG++P+L+
Sbjct 5 IIKVHWLDHSRAFRLLWLLDHLNLEYEIVPYKRDANFRAPPELKKIHPLGRSPLLEVQDR 64
Query 71 --DNQRVIAESAVILEYLQSQYDLDHQFKPQNDEDAFQYLYWMHYAEGSLMPLLVFQLVM 128
++++AES I +Y+ +D H ++ + A Q Y++ Y EGSL P L+ + ++
Sbjct 65 ETGKKKILAESGFIFQYVLQHFDHSHVLMSEDADIADQINYYLFYVEGSLQPPLMIEFIL 124
Query 129 NNV-GKNVPFLIRPVANKITDGIKASFIRPRLKDHIAFLEDYLSKHDYFA--GQFSFADI 185
+ V +PF I +A K+ D I ++ +K+ F+E +SK++ + G+ S ADI
Sbjct 125 SKVKDSGMPFPISYLARKVADKISQAYSSGEVKNQFDFVEGEISKNNGYLVDGKLSGADI 184
Query 186 QMSFPLEAILTR---TTGQYPHIQAFLKRIQVRPAYQQAKQK 224
MSFPL+ R YP I +LK I +Y +K+K
Sbjct 185 LMSFPLQMAFERKFAAPEDYPAISKWLKTITSEESYAASKEK 226
>Q9P6M1 Glutathione S-transferase 3 [Schizosaccharomyces pombe
(strain 972 / ATCC 24843)]
Length=242
Score = 132 bits (331), Expect = 2e-41
Identities = 80/215 (37%), Positives = 125/215 (58%), Gaps = 8/215 (4%)
Query 15 MLTLHHLEQSRSFRILWALEELQQNYEIKYYKRSPKFAAPPELKQVHPLGKAPILQDNQR 74
M+ LHHL+ SRS RI+W LEEL+ YEIK Y R APP ++ PLGK+PI+ D+
Sbjct 1 MIVLHHLKNSRSTRIVWMLEELKVPYEIKVYDRVDG-RAPPAYTKLSPLGKSPIVVDDGV 59
Query 75 VIAESAVILEYLQSQYDLDHQFKPQNDEDAF--QYLYWMHYAEGSLMPLLVFQLVMNNVG 132
ESA ILE+L +Y FKP ++ A +Y WMH++E SLMP + V++
Sbjct 60 TYIESAAILEHLVRKYG--PSFKPSEEDVAELEKYELWMHFSEASLMPFIWASHVLDLSV 117
Query 133 KNVPFLIRPVANKITDGIKASFIRPRLKDHIAFLEDYLSKHDYFAG-QFSFADIQMSFPL 191
P R + + +GIK+ ++ ++ +++++L+ ++YFAG QF+ AD QM FP+
Sbjct 118 NMTPIFFRYIVRQFVNGIKSKYLSKETFLNLDYIDNHLASNEYFAGEQFTAADPQMCFPI 177
Query 192 EAILTRTTGQ--YPHIQAFLKRIQVRPAYQQAKQK 224
A Q Y +I+ +++ + RPA + A +K
Sbjct 178 FAAQRDYLSQKPYKNIKRWMRVVSDRPACRIAAEK 212
>Q9Y7Q2 Glutathione S-transferase 1 [Schizosaccharomyces pombe
(strain 972 / ATCC 24843)]
Length=229
Score = 52.8 bits (125), Expect = 5e-12
Identities = 47/174 (27%), Positives = 72/174 (41%), Gaps = 23/174 (13%)
Query 20 HLEQSRSFRILWALEELQQNYEIKYYKRSPKFAAPPELKQVHPLGKAPILQD---NQRVI 76
H ++++ AL+EL YE +Y S PE ++P G+ P L D N I
Sbjct 9 HAHGPNPWKVVQALKELDLTYETRYVNFSKNEQKSPEHLALNPNGRVPTLIDHHNNDYTI 68
Query 77 AESAVILEYLQSQYDLDHQFKPQNDEDAF----QYLYWMHYAEGSLMPLLVFQLVMNNVG 132
ES IL YL +YD + + D + QYL++ +G ++ G
Sbjct 69 WESDAILIYLADKYDTERKISLPRDHPEYYKVIQYLFFQASGQG---------IIWGQAG 119
Query 133 KNVPFLIRPVANKITDGIKASFIRPRLKDHIAFLEDYLSKHDYF-AGQFSFADI 185
+ V + IT R +K + LED L DY A +F+ AD+
Sbjct 120 WFSVYHQELVISAITR------YRNEIKRVLGVLEDILKDRDYLVANRFTIADL 167
>Q52828 Protein GstA [Rhizobium leguminosarum]
Length=203
Score = 43.1 bits (100), Expect = 9e-09
Identities = 29/104 (28%), Positives = 46/104 (44%), Gaps = 1/104 (1%)
Query 15 MLTLHHLEQSRSFRILWALEELQQNYEIKYYKRSPKFAAPPELKQVHPLGKAPILQDNQR 74
M HH + R L L YE+ + P+ +++P G+ P+L DN
Sbjct 1 MKLYHHPLSGHAHRAHLFLSLLGVPYELVEVDLAAGAHKAPDFLKLNPFGQVPVLDDNGT 60
Query 75 VIAESAVILEYLQSQYDLDHQFKPQNDEDAFQYLYWMHYAEGSL 118
VIA+S+ IL YL +Y + P+ A + W+ A G +
Sbjct 61 VIADSSAILVYLARKYGRT-DWLPEEAVAAARIQKWLSVAAGEI 103
>P30347 Protein LigF [Sphingobium sp. (strain NBRC 103272 / SYK-6)]
Length=257
Score = 43.1 bits (100), Expect = 1e-08
Identities = 45/198 (23%), Positives = 84/198 (42%), Gaps = 32/198 (16%)
Query 58 KQVHPLGKAPILQDNQRVIAESAVILEYLQSQY-DLDHQFKPQNDEDAFQYLYWMHYAEG 116
K+++P G+ P L + +V+ ES VI EYL+ + + + +P + + W + +
Sbjct 46 KKINPRGQVPALWHDGKVVTESTVICEYLEDVFPESGNSLRPADPFKRAEMRVWTKWVDE 105
Query 117 ----------------SLMPLLVFQLVMNNVGKNVPFLIRPVA-NKITDGIKASFIRPRL 159
++ + + ++ KNVP + + + +G +
Sbjct 106 YFCWCVSTIGWAFGIKAIAQKMSDEEFEEHINKNVPIPEQQLKWRRARNGFPQEMLDEEF 165
Query 160 KD---HIAFLEDYLSKHDYFAGQ-FSFADIQMSFPLEAILTRTTGQY---------PHIQ 206
+ +A LE+ LSK DY +S ADI +F + L R G + P +
Sbjct 166 RKVGVSVARLEETLSKQDYLVDTGYSLADI-CNFAIANGLQRPGGFFGDYVNQEKTPGLC 224
Query 207 AFLKRIQVRPAYQQAKQK 224
A+L RI RPA ++ +K
Sbjct 225 AWLDRINARPAIKEMFEK 242
>Q9SR36 Glutathione S-transferase U8 [Arabidopsis thaliana]
Length=224
Score = 42.7 bits (99), Expect = 1e-08
Identities = 22/66 (33%), Positives = 35/66 (53%), Gaps = 1/66 (2%)
Query 55 PELKQVHPLGK-APILQDNQRVIAESAVILEYLQSQYDLDHQFKPQNDEDAFQYLYWMHY 113
P L + +P+ K P+L N R IAES VI+EY++ + H PQ+ + +W Y
Sbjct 45 PMLLKYNPIHKKVPVLIHNGRSIAESLVIVEYIEDTWKTTHTILPQDPYERAMARFWAKY 104
Query 114 AEGSLM 119
+ +M
Sbjct 105 VDEKVM 110
Score = 15.8 bits (29), Expect = 9.9
Identities = 8/24 (33%), Positives = 11/24 (46%), Gaps = 0/24 (0%)
Query 131 VGKNVPFLIRPVANKITDGIKASF 154
VG N + P K+ +KA F
Sbjct 195 VGNNFIKEVLPPKEKLVAVLKAMF 218
>Q4WQZ2 Glutathione S-transferase-like protein tpcF [Aspergillus
fumigatus (strain ATCC MYA-4609 / CBS 101355 / FGSC A1100
/ Af293)]
Length=225
Score = 42.7 bits (99), Expect = 1e-08
Identities = 47/195 (24%), Positives = 76/195 (39%), Gaps = 51/195 (26%)
Query 55 PELKQVHPLGKAPILQD--NQRVIAESAVILEYLQSQYDLDHQFK-PQNDEDAFQYLYWM 111
P+ ++P G+ P + D + ES I+EYL S YD DH+ P A W+
Sbjct 44 PDYVAINPNGRIPAIYDPNTDLTLWESGAIVEYLVSHYDPDHRISFPAGSNLAALATQWL 103
Query 112 ---------HYAEGSLMPLLVFQLVMNNVGKNVPFLIRPVANKITDGIKASFIRPRLKDH 162
+Y + S + V + + + V + N++T
Sbjct 104 FFQASGQGPYYGQASWFKKFHHEKVPSAIERYVKEI-----NRVT--------------- 143
Query 163 IAFLEDYLSKHDYFA---------GQFSFADIQMSFPLEAILTR--------TTGQYPHI 205
LE +LS+ A G+ SFAD+ P + I+T T YPH+
Sbjct 144 -GVLEGHLSRQKVAADGDGPWLVGGKCSFADLAW-IPWQVIVTAIIQPEDGYTVEDYPHV 201
Query 206 QAFLKRIQVRPAYQQ 220
+ +L R+ RP Q+
Sbjct 202 KNWLDRMMARPGVQK 216
>Q9ZW29 Glutathione S-transferase U2 [Arabidopsis thaliana]
Length=225
Score = 41.2 bits (95), Expect = 5e-08
Identities = 28/124 (23%), Positives = 55/124 (44%), Gaps = 22/124 (18%)
Query 56 ELKQVHPLGKAPILQDNQRVIAESAVILEYLQSQYDLDHQFKPQNDEDAFQYLYWMHYAE 115
EL VH K P+L N ++++ES VILEY+ ++ ++ P + + +W + +
Sbjct 49 ELNPVHK--KVPVLVHNDKLLSESHVILEYIDQTWN-NNPILPHDPYEKAMVRFWAKFVD 105
Query 116 GSLMPLLVFQLVMNNVGKNVPFLIRPVANKITDGIKASFIRPRLKDHIAFLEDYLSKHDY 175
++P+ LV G +V +++ + FLE ++ D+
Sbjct 106 EQILPVGFMPLVKAEKGIDVAI-------------------EEIREMLMFLEKEVTGKDF 146
Query 176 FAGQ 179
F G+
Sbjct 147 FGGK 150
Score = 18.5 bits (36), Expect = 1.3
Identities = 10/27 (37%), Positives = 12/27 (44%), Gaps = 0/27 (0%)
Query 113 YAEGSLMPLLVFQLVMNNVGKNVPFLI 139
Y E L L +N V K VP L+
Sbjct 35 YLEEDLPKKSTLLLELNPVHKKVPVLV 61
>B3FWR8 Glutathione S-transferase hmp2 [Hypomyces subiculosus]
Length=212
Score = 38.9 bits (89), Expect = 3e-07
Identities = 49/206 (24%), Positives = 80/206 (39%), Gaps = 24/206 (12%)
Query 28 RILWALEELQQNYEIKYYKRSPKFAAPPELKQVHPLGKAPILQDNQRVIAESAVILEYLQ 87
R+L L E + YE S E ++ P GK P+L DN ++ ES I YL
Sbjct 14 RVLVTLLEKELPYEHILVDISKGDQNKEEYLKLQPFGKVPVLDDNGFIMYESRAICRYLA 73
Query 88 SQYDLDHQFKPQNDEDAFQYLYWMHYAEGSLMPLLVFQLVMNNVG-----KNVPFLIRPV 142
+YD + P D+ + + + +G + F +G K L P
Sbjct 74 RKYDSGTKLIPDVDD----HEAYGRFEQGCSIEYSYFAAAAETLGTELVIKKYKGLGEPD 129
Query 143 ANKITDGIKASFIRPRLKDHIAFLED-YLSKHDYFAG-QFSFADIQMSFPLEAILTR--- 197
++ +A F D + F D L+K Y G + S D+ P + L
Sbjct 130 MTRVAQA-EADF------DKVFFEYDKILAKQKYLTGDEISLVDL-FHLPNASALKAFGY 181
Query 198 --TTGQYPHIQAFLKRIQVRPAYQQA 221
T +YP++ + +Q R + +A
Sbjct 182 QGTFEKYPNVNRWFSGLQARETWIKA 207
>Q9CA57 Glutathione S-transferase U10 [Arabidopsis thaliana]
Length=232
Score = 38.5 bits (88), Expect = 4e-07
Identities = 29/117 (25%), Positives = 53/117 (45%), Gaps = 9/117 (8%)
Query 56 ELKQVHPLGKAPILQDNQRVIAESAVILEYLQSQYDLDHQFKPQNDEDAFQYLYWMHYAE 115
+L VH K P+L + + +AES VILEY+ + +F P++ + Q +W+ Y
Sbjct 49 QLNPVHK--KIPVLVHDGKPVAESLVILEYIDETWTNSPRFFPEDPYERAQVRFWVSYIN 106
Query 116 GSLMPLLVFQLVMNNVGKNVPFLIRPVANK---ITDGIKASFIRPRLK--DHIAFLE 167
+ V VM+ G+ + + + +G+K F ++ D + LE
Sbjct 107 QQVFE--VMGQVMSQEGEAQAKSVEEARKRFKVLDEGLKKHFPNKNIRRNDDVGLLE 161
Score = 15.8 bits (29), Expect = 9.9
Identities = 11/38 (29%), Positives = 15/38 (39%), Gaps = 0/38 (0%)
Query 190 PLEAILTRTTGQYPHIQAFLKRIQVRPAYQQAKQKGIE 227
P E R Y + Q F QV +A+ K +E
Sbjct 92 PYERAQVRFWVSYINQQVFEVMGQVMSQEGEAQAKSVE 129
>P46417 Glutathione S-transferase 3 [Glycine max]
Length=219
Score = 38.1 bits (87), Expect = 5e-07
Identities = 25/85 (29%), Positives = 40/85 (47%), Gaps = 4/85 (5%)
Query 32 ALEELQQNYEIKYYKRSPKFAAPPELKQVHPLGKA-PILQDNQRVIAESAVILEYLQSQY 90
AL E YE YK P L Q++P+ K P+L N + I ESA+I++Y+ +
Sbjct 22 ALAEKGVRYE---YKEENLMNRSPLLLQMNPIHKKIPVLIHNGKPICESAIIVQYIDEVW 78
Query 91 DLDHQFKPQNDEDAFQYLYWMHYAE 115
+ P + Q +W+ Y +
Sbjct 79 NDKSPLMPSDPYKRSQARFWVDYID 103
>Q9ZW30 Glutathione S-transferase U1 [Arabidopsis thaliana]
Length=224
Score = 37.7 bits (86), Expect = 7e-07
Identities = 28/126 (22%), Positives = 54/126 (43%), Gaps = 21/126 (17%)
Query 55 PELKQVHPLGK-APILQDNQRVIAESAVILEYLQSQYDLDHQFKPQNDEDAFQYLYWMHY 113
P L +++PL K P+L N +++ ES +ILEY+ + + PQ+ + +W +
Sbjct 45 PLLLELNPLHKKVPVLVHNDKILLESHLILEYIDQTWK-NSPILPQDPYEKAMARFWAKF 103
Query 114 AEGSLMPLLVFQLVMNNVGKNVPFLIRPVANKITDGIKASFIRPRLKDHIAFLEDYLSKH 173
+ ++ L LV G+ V ++ + FLE ++
Sbjct 104 IDDQILTLGFRSLVKAEKGREVAI-------------------EETRELLMFLEKEVTGK 144
Query 174 DYFAGQ 179
D+F G+
Sbjct 145 DFFGGK 150
Score = 16.5 bits (31), Expect = 5.6
Identities = 9/27 (33%), Positives = 12/27 (44%), Gaps = 0/27 (0%)
Query 113 YAEGSLMPLLVFQLVMNNVGKNVPFLI 139
Y E L L +N + K VP L+
Sbjct 35 YLEEDLPNKTPLLLELNPLHKKVPVLV 61
>P46430 Glutathione S-transferase 1 [Manduca sexta]
Length=217
Score = 37.4 bits (85), Expect = 9e-07
Identities = 44/218 (20%), Positives = 88/218 (40%), Gaps = 20/218 (9%)
Query 14 LMLTLHHLEQSRSFRILWALEELQQNYEIKYYKRSPKFAA--PPELKQVHPLGKAPILQD 71
+++TL+ L+ S R + + E + +++Y + + E +++P P+L+D
Sbjct 1 MVMTLYKLDASPPARAVMMVIEALKIPDVEYIDVNLLEGSHLSEEFTKMNPQHTVPLLKD 60
Query 72 NQRVIAESAVILEYLQSQYDLDHQFKPQNDEDAFQYLYWMHYAEGSLMPLLVFQLVMNNV 131
+ ++ +S I YL S+Y D P + + +H+ G L P L L
Sbjct 61 DDFLVWDSHAIAGYLVSKYGADDSLYPTDPKKRAIVDQRLHFDSGILFPALRGSL----- 115
Query 132 GKNVPFLIRPVANKITDGIKASFIRPRLKDHIAFLEDYLSKHDYFAGQFSFADI----QM 187
PV + + +++ F E +L+ +F+ ADI +
Sbjct 116 --------EPVIFWGETAFRPECLE-KVRKGYDFAEKFLTSTWMAGEEFTVADICCVASI 166
Query 188 SFPLEAILTRTTGQYPHIQAFLKRIQVRPAYQQAKQKG 225
S + I+ YP + A+L+R Y++ G
Sbjct 167 STMNDIIVPIDENTYPKLSAWLERCSQLDVYKKKNAPG 204
>P32111 Probable glutathione S-transferase [Solanum tuberosum]
Length=217
Score = 37.4 bits (85), Expect = 9e-07
Identities = 29/91 (32%), Positives = 41/91 (45%), Gaps = 5/91 (5%)
Query 26 SFRILWALEELQQNYEIKYYKRSPKFAAPPELKQVHPLGKA-PILQDNQRVIAESAVILE 84
S R+ WAL+ YE K P L Q +P+ K P+L N + I ES VILE
Sbjct 15 SHRVEWALKIKGVKYEFIEEDLQNK---SPLLLQSNPIHKKIPVLIHNGKCICESMVILE 71
Query 85 YLQSQYDLDHQFKPQNDEDAFQYLYWMHYAE 115
Y+ ++ P++ D +W Y E
Sbjct 72 YIDEAFE-GPSILPKDPYDRALARFWAKYVE 101
Lambda K H a alpha
0.325 0.139 0.416 0.792 4.96
Gapped
Lambda K H a alpha sigma
0.267 0.0410 0.140 1.90 42.6 43.6
Effective search space used: 488800
Database: a8153455ce498e0a073dedb0b9d2e8aa.SwissProt.fasta
Posted date: Jun 3, 2024 6:31 PM
Number of letters in database: 3,160
Number of sequences in database: 14
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 11
Window for multiple hits: 40
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Data ...
