BLASTP 2.12.0+
Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics: Alejandro A. Schaffer,
L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri
I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids
Res. 29:2994-3005.
Database: ec9748a2c90475c5ba47ba1c5fe05e3d.SwissProt.fasta
6 sequences; 1,732 total letters
Query= ACIAD2484
Length=318
Score E
Sequences producing significant alignments: (Bits) Value
Q02ML7 CRISPR-associated endonuclease Cas1 [Pseudomonas aeruginos... 239 3e-81
Q6D0X0 CRISPR-associated endonuclease Cas1 [Pectobacterium atrose... 233 7e-79
Q2RY21 CRISPR-associated endonuclease Cas1 1 [Rhodospirillum rubr... 62.0 1e-14
Q2FNT7 CRISPR-associated endonuclease Cas1 3 [Methanospirillum hu... 51.6 3e-11
Q46896 CRISPR-associated endonuclease Cas1 [Escherichia coli (str... 48.9 2e-10
Q20222 Fatty acid-binding protein homolog 3 [Caenorhabditis elegans] 35.4 2e-06
>Q02ML7 CRISPR-associated endonuclease Cas1 [Pseudomonas aeruginosa
(strain UCBPP-PA14)]
Length=324
Score = 239 bits (609), Expect = 3e-81
Identities = 130/327 (40%), Positives = 195/327 (60%), Gaps = 20/327 (6%)
Query 1 MSQLRQERSRPVMLSKRACVFYLEKVRVVLKDDRIVYLTDNSQPVEYFYNIPEKNTAFLL 60
M + + ++ SKRA ++YL+ RV++ R+ Y+TD + Y +NIP NT LL
Sbjct 1 MDDISPSELKTILHSKRANLYYLQHCRVLVNGGRVEYVTDEGRHSHY-WNIPIANTTSLL 59
Query 61 LGKGSSLTDAAARRLAESNVMVGFCGSGGSPLFSS----LDLTFLAPQSEYRPTEYMQIW 116
LG G+S+T AA R LA + V+VGFCG GG+PLFS+ +++++L PQSEYRPTEY+Q W
Sbjct 60 LGTGTSITQAAMRELARAGVLVGFCGGGGTPLFSANEVDVEVSWLTPQSEYRPTEYLQRW 119
Query 117 MKAWLDDDARLLMAKILLLERIVLVSQSWKKNLDLIQHGIYIDENALNTFKQD----IDR 172
+ W D++ RL+ A+ R+ + SW ++ L G +D AL +D +++
Sbjct 120 VGFWFDEEKRLVAARHFQRARLERIRHSWLEDRVLRDAGFAVDATALAVAVEDSARALEQ 179
Query 173 SENQQELLAAEGRWAKALYKELAK----GFNIDFKRDEGKNSHDSLTDIANNYLDHGNYI 228
+ N + LL E R +K L+K A+ G + KR G D AN +LDHGNY+
Sbjct 180 APNHEHLLTEEARLSKRLFKLAAQATRYGEFVRAKRGSGG-------DPANRFLDHGNYL 232
Query 229 AYGYAAVALNGMGISFALPILHGKTRRGGLVFDLADLVKDAFVMPTAFICASKGLNQKEF 288
AYG AA A +GI L +LHGKTRRGGLVFD+ADL+KD+ ++P AF+ A +G +++F
Sbjct 233 AYGLAATATWVLGIPHGLAVLHGKTRRGGLVFDVADLIKDSLILPQAFLSAMRGDEEQDF 292
Query 289 RMQLIEICQDQDLLDYMFSFITDICSK 315
R ++ LD+M + D+ +
Sbjct 293 RQACLDNLSRAQALDFMIDTLKDVAQR 319
>Q6D0X0 CRISPR-associated endonuclease Cas1 [Pectobacterium atrosepticum
(strain SCRI 1043 / ATCC BAA-672)]
Length=326
Score = 233 bits (594), Expect = 7e-79
Identities = 127/316 (40%), Positives = 190/316 (60%), Gaps = 14/316 (4%)
Query 10 RPVMLSKRACVFYLEKVRVVLKDDRIVYLTDNSQPVEYFYNIPEKNTAFLLLGKGSSLTD 69
+ ++ SKRA V+YL+ R+++ R+ Y+T+ Y +NIP NT+ ++LG G+S+T
Sbjct 11 KTILHSKRANVYYLQHCRILVNGGRVEYVTEEGNQSLY-WNIPIANTSVVMLGTGTSVTQ 69
Query 70 AAARRLAESNVMVGFCGSGGSPLFSSLD----LTFLAPQSEYRPTEYMQIWMKAWLDDDA 125
AA R A + VM+GFCG GG+PLF++ + +++L+PQSEYRPTEY+Q W+ W DD+
Sbjct 70 AAMREFARAGVMIGFCGGGGTPLFAANEAEVAVSWLSPQSEYRPTEYLQDWVSFWFDDEK 129
Query 126 RLLMAKILLLERIVLVSQSWK-KNLDLIQHGIYIDENA---LNTFKQDIDRSENQQELLA 181
RL A RI + Q W L + E+ L+ +++ + ++L
Sbjct 130 RLAAAIAFQQVRITQIRQHWLGSRLSRESRFTFKSEHLQALLDRYQKGLTDCRTSNDVLV 189
Query 182 AEGRWAKALYKELAKGFNI-DFKRDEGKNSHDSLTDIANNYLDHGNYIAYGYAAVALNGM 240
E KALY+ A + DF R + TD+AN +LDHGNY+AYG AAV+ +
Sbjct 190 QEAMMTKALYRLAANAVSYGDFTRAKRGGG----TDLANRFLDHGNYLAYGLAAVSTWVL 245
Query 241 GISFALPILHGKTRRGGLVFDLADLVKDAFVMPTAFICASKGLNQKEFRMQLIEICQDQD 300
G+ L +LHGKTRRGGLVFD+ADL+KDA V+P AFI A +G +++EFR + + Q +
Sbjct 246 GLPHGLAVLHGKTRRGGLVFDVADLIKDALVLPQAFIAAMEGEDEQEFRQRCLTAFQQSE 305
Query 301 LLDYMFSFITDICSKI 316
LD M + D+ SK+
Sbjct 306 ALDVMIGSLQDVASKL 321
>Q2RY21 CRISPR-associated endonuclease Cas1 1 [Rhodospirillum
rubrum (strain ATCC 11170 / ATH 1.1.1 / DSM 467 / LMG 4362 /
NCIMB 8255 / S1)]
Length=313
Score = 62.0 bits (149), Expect = 1e-14
Identities = 70/295 (24%), Positives = 118/295 (40%), Gaps = 46/295 (16%)
Query 10 RPVMLSKRACVFYLEKVRVVLKDDRIVYLTDNSQPVEYFYNIPEKNTAFLLLGKGSSLTD 69
RP+ + R+ + +L++ ++ + D V + V+ IP A L+L G+ +T
Sbjct 10 RPIAIKDRSSIVFLQRGQLDVVDGAFVLIDQEGVRVQ----IPVGGLACLMLEPGTRITH 65
Query 70 AAARRLAESNVMVGFCGSGGSPLFSSLDLTFLAPQSEYRPTEYMQIWMKAWLDDDARLLM 129
AA A +V + G G+ L++ A Q + + + LD+ ARL +
Sbjct 66 AAIVLCARVGCLVIWVGERGTRLYA-------AGQPGGARADRLLFQARNALDETARLNV 118
Query 130 AKILLLERIVLVSQSWKKNLDLIQHGIYIDENALNTFKQDIDRSENQQELLAAEGRWAKA 189
+ + R F D + +L EG +
Sbjct 119 VREMYRRR----------------------------FDDDPPARRSVDQLRGMEGVRVRE 150
Query 190 LYKELAKGFNIDFKRDEGKNSHDSLTDIANNYLDHGNYIAYGYAAVALNGMGISFALPIL 249
+Y+ LAK + +D+ ++ DI N L YG A+ G + A+ L
Sbjct 151 IYRLLAKKYAVDWNARRYDHNDWDGADIPNRCLSAATACLYGLCEAAILAAGYAPAIGFL 210
Query 250 HGKTRRGGLVFDLADLVKDAFVMPTAF-----ICASKGLNQKEFRMQLIEICQDQ 299
H + + V+D+ADL K V+PTAF I A KG + R Q+ C+DQ
Sbjct 211 H-RGKPQSFVYDVADLYKVETVVPTAFSIAAKIAAGKGDDSPPER-QVRIACRDQ 263
>Q2FNT7 CRISPR-associated endonuclease Cas1 3 [Methanospirillum
hungatei JF-1 (strain ATCC 27890 / DSM 864 / NBRC 100397 /
JF-1)]
Length=299
Score = 51.6 bits (122), Expect = 3e-11
Identities = 67/290 (23%), Positives = 115/290 (40%), Gaps = 44/290 (15%)
Query 10 RPVMLSKRACVFYLEKVRVVLKDDRIVYLTDNSQPVEYFYNIPEKNTAFLLLGKGSSLTD 69
+P+ + +R+ V +L + + + D V + N ++ IP A L+L GS ++
Sbjct 9 KPIPIKERSSVVFLGRGELDVIDGAFVLVDTNGIRMQ----IPVGGLASLMLEPGSRVSH 64
Query 70 AAARRLAESNVMVGFCGSGGSPLFSSLDLTFLAPQSEYRPTEYMQIWMKAWLDDDARLLM 129
AA ++ ++ F G GG L+S + D L
Sbjct 65 AAVSLASKVGCLLVFVGEGGVRLYS--------------------VGHPGGARSDRLLYQ 104
Query 130 AKILLLERIVLVSQSWKKNLDLIQHGIYIDENALNTFKQDIDRSENQQELLAAEGRWAKA 189
A++ L E VL + KK L F +D + + ++L EG +
Sbjct 105 ARLALDE--VLRLKVVKKMFSL-------------RFGEDFSDAYSVEQLRGLEGVRVRE 149
Query 190 LYKELAKGFNIDFKRDEGKNSHDSLTDIANNYLDHGNYIAYGYAAVALNGMGISFALPIL 249
Y+++A+ + + D+ N L YG A+ G S ++ L
Sbjct 150 GYRKIARDTGVIWNGRRYDPHSWGSADLPNRCLSAATASLYGICEAAVLAAGYSPSIGFL 209
Query 250 H-GKTRRGGLVFDLADLVKDAFVMPTAFICASKGLNQKEFRMQLIEICQD 298
H GK V+D+ADL K V+P AF A+ LN +E ++ C+D
Sbjct 210 HTGKPL--SFVYDIADLFKFETVVPAAFKTAA--LNPREPEREVRYACRD 255
>Q46896 CRISPR-associated endonuclease Cas1 [Escherichia coli
(strain K12)]
Length=305
Score = 48.9 bits (115), Expect = 2e-10
Identities = 62/305 (20%), Positives = 116/305 (38%), Gaps = 44/305 (14%)
Query 11 PVMLSKRACVFYLEKVRVVLKDDRIVYLTDNSQPVEYFYNIPEKNTAFLLLGKGSSLTDA 70
P+ L R + +L+ ++ + D V + +IP + A ++L G+ ++ A
Sbjct 8 PIPLKDRVSMIFLQYGQIDVIDGAFVLIDKTGIRT----HIPVGSVACIMLEPGTRVSHA 63
Query 71 AARRLAESNVMVGFCGSGGSPLFSSLDLTFLAPQSEYRPTEYMQIWMKAWLDDDARLLMA 130
A R A+ ++ + G G +++S Q ++ + K LD+D RL +
Sbjct 64 AVRLAAQVGTLLVWVGEAGVRVYAS-------GQPGGARSDKLLYQAKLALDEDLRLKVV 116
Query 131 KILLLERIVLVSQSWKKNLDLIQHGIYIDENALNTFKQDIDRSENQQELLAAEGRWAKAL 190
+ + R F + + ++L EG +A
Sbjct 117 RKMFELR----------------------------FGEPAPARRSVEQLRGIEGSRVRAT 148
Query 191 YKELAKGFNIDFKRDEGKNSHDSLTDIANNYLDHGNYIAYGYAAVALNGMGISFALPILH 250
Y LAK + + + D N + YG A+ G + A+ +H
Sbjct 149 YALLAKQYGVTWNGRRYDPKDWEKGDTINQCISAATSCLYGVTEAAILAAGYAPAIGFVH 208
Query 251 -GKTRRGGLVFDLADLVKDAFVMPTAFICASK--GLNQKEFRMQLIEICQDQDLLDYMFS 307
GK V+D+AD++K V+P AF A + G +E R+ +I + L +
Sbjct 209 TGKPL--SFVYDIADIIKFDTVVPKAFEIARRNPGEPDREVRLACRDIFRSSKTLAKLIP 266
Query 308 FITDI 312
I D+
Sbjct 267 LIEDV 271
>Q20222 Fatty acid-binding protein homolog 3 [Caenorhabditis elegans]
Length=165
Score = 35.4 bits (80), Expect = 2e-06
Identities = 28/76 (37%), Positives = 40/76 (53%), Gaps = 3/76 (4%)
Query 167 KQDIDRSENQQELLAAEG-RWAKALYKELAKGFNIDFKRD-EGKNSHDSLTDIANNYLDH 224
K D+DRSEN E LAA+G W +LAK + K + GK + ++LT N L H
Sbjct 30 KYDLDRSENFDEFLAAKGVSWFVRQMIKLAKVSKVLAKNETPGKYNMENLTS-KKNTLYH 88
Query 225 GNYIAYGYAAVALNGM 240
G + + A L+G+
Sbjct 89 GWELGKTFEAEGLDGV 104
Lambda K H a alpha
0.324 0.139 0.411 0.792 4.96
Gapped
Lambda K H a alpha sigma
0.267 0.0410 0.140 1.90 42.6 43.6
Effective search space used: 417942
Database: ec9748a2c90475c5ba47ba1c5fe05e3d.SwissProt.fasta
Posted date: May 20, 2024 10:29 PM
Number of letters in database: 1,732
Number of sequences in database: 6
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 11
Window for multiple hits: 40
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Data ...
